Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil     

Macieira Dde B  Messick JB  Cerqueira Ade M  Freire IM  Linhares GF  Almeida NK  Almosny NR 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(1):44-48

BACKGROUND: Infection with Ehrlichia canis causes a highly variable, multisystemic disease in dogs. Nevertheless, many clinicians in Rio de Janeiro, Brazil, use the presence of only thrombocytopenia to make a presumptive diagnosis of E canis infection. OBJECTIVE: The objective of this study was to determine the prevalence of E canis in thrombocytopenic dogs from Rio de Janeiro, Brazil, using polymerase chain reaction (PCR). METHODS: Following DNA extraction of whole blood samples from 226 dogs, PCR assays were done using primers for rickettsial DNA (including Ehrlichia spp, Anaplasma platys and A phagocytophilum) and using E canis-specific primers (16S rRNA gene). Dogs were grouped as thrombocytopenic and nonthrombocytopenic based on platelet counts. The null hypothesis that there was no difference in the prevalence of E canis in these groups was rejected at P<.05. RESULTS: Thirty-six (32.1%) of the thrombocytopenic dogs and 4 (3.5%) of the nonthrombocytopenic dogs were positive for rickettsial gene sequences (P<.0001). Further, 30 (26.8%) of thrombocytopenic dogs and 4 (3.5%) nonthrombocytopenic dogs were positive for E canis-specific gene sequences (P<.0001). CONCLUSIONS: Although the prevalence of E canis infection was higher in thrombocytopenic dogs, less than one third of these dogs had demonstrable E canis infection. Thus, thrombocytopenia is not specific for the detection of E canis infection and should not be used solely to establish a diagnosis of canine ehrlichiosis, even in a geographic area with relatively high disease prevalence.  相似文献   

Direct and correlated responses to selection for yearling weight on reproductive performance of Nelore cows     

Mercadante ME  Packer IU  Razook AG  Cyrillo JN  Figueiredo LA 《Journal of animal science》2003,81(2):376-384

Data from a selection experiment for growth carried out in Brazil were analyzed in order to evaluate the direct responses on yearling weight (YW) and the correlated responses on the size and reproduction traits of cows. The experiment was started in 1976, and in 1980 three lines of Nelore cattle were established: selection (NeS), traditional (NeT), both selected for higher YW, and control (NeC), selected for mean YW. The NeT was an open line that eventually received bulls from other herds. Yearling weight records for animals born from 1978 to 1998 and yearling hip height (H550) offemales born from 1985 to 1998 were analyzed by fitting an animal model in order to obtain the genetic trends. The means for weight, height, and body condition score at the start of the breeding season, days to calving, and calving success of cows born from 1993 to 1996 (pertaining to the third to fourth generations of selection) were compared between the selected (NeS and NeT) and control lines. The genetic trends obtained after 16 yr for YW were 1.7 +/- 0.2, 2.3 +/- 0.2, and -0.1 +/- 0.1 kg/yr for males and 1.9 +/- 0.2, 2.4 +/- 0.2, and -0.1 +/- 0.1 kg/yr for females, for the NeS, NeT, and NeC lines, respectively. Corresponding values for H550 were 0.25 +/- 0.03, 0.24 +/- 0.04 and -0.04 +/- 0.03 cm/yr for females. Heifers and cows from NeS and NeT were 19% and 15% heavier and 4% taller at the start of the breeding season than those from NeC. No significant differences between selected (NeS and NeT) and control females were detected for body condition scores and for reproductive performance. The results indicate that selection for body weight promoted high and consistent weight and height responses both at the yearling and later ages, without compromising the reproductive performance of the cows with respect to days to calving and calving success.  相似文献   

Preliminary study of short-term preservation of ovine ovarian tissue containing preantral follicles in saline solution of TCM199     

Andrade ER  Amorim CA  Costa SH  Ferreira MA  Rodrigues AP  Dode MA  Figueiredo JR 《The Veterinary record》2002,151(15):452-453

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Morphological aspects of the capybara stomach (Hydrochaeris hydrochaeris): gross and microscopic structure     

Moraes PT  Pacheco MR  de Souza WM  da Silva RA  Neto PB  Barreto CS  Ribeiro AA 《Anatomia, histologia, embryologia》2002,31(6):362-366

The digestive system of the capybara has been investigated because of its coprofagia habits, important for their absorptive activity. These species present differences in terms of gastrointestinal morphological characters when compared with other rodents. Macroscopiclly, the stomach of the capybara is constituted of the following parts: cardiac, pyloric, body, fundic and gastric diverticulum. It presents two curvatures, one big and another small. Externally, the presence of gastric bands (tenias) is observed. With regards to the volumetric view, the gastric capacity varies from 850 to 2010 ml, with an average of 1498.57 ml. So, the stomach of this animal can be classified as a simple stomach, in the format of a curved sack and similar to an inverted letter 'J'. The gastric mucous membrane presents a surface filled by numerous tortuous gastric folds and longitudinally distributed along all its extension. The mucous tunic also possesses recesses located among the successive gastric folds, which were denoted as gastric parts with numerous openings described as gastric pits. In the cardiac part, a glandular epithelium with cardiac glands is noticed containing a lot of parietal and mucous neck cells. The fundic part, body and gastric diverticulum contain proper gastric glands with main, parietal and mucous neck cells. Finally, the pyloric part has pyloric glands with two cellular types, mucous neck and parietal cells.  相似文献   

CLINICAL USE OF A COMBINATION OF NEUROLEPTANALGESIA IN DOGS WITH DISSOCIATIVE ANAESTHESIA: TILETAMINE-ZOLAZEPAM     

M.P. Holzchuh  A.C.C. Figueiredo  S.R.G. Cortopassi 《Veterinary anaesthesia and analgesia》1991,18(Z1):183-184

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Sedative, analgesic and cardiorespiratory effects of romifidine in cats     

Selmi AL  Barbudo-Selmi GR  Mendes GM  Figueiredo JP  Lins BT 《Veterinary anaesthesia and analgesia》2004,31(3):195-206

OBJECTIVE: To evaluate the sedative, analgesic, and cardiorespiratory effects of intramascular (IM) romifidine in cats. STUDY DESIGN: Prospective, randomized experimental trial. ANIMALS: Ten healthy adult cats. METHODS: Romifidine (100, 200, and 400 microg kg(-1)) or xylazine (1 mg kg(-1)) was given IM in a cross-over study design. Heart rate (HR), respiratory rate (RR), rectal temperature (RT), hemoglobin saturation, oscillometric arterial pressure, and scores for sedation, muscle relaxation, position, auditory response, and analgesia were determined before and after drug administration. Time to recumbency, duration of recumbency, and time to recover from sedation were determined. Subjective evaluation and cardiorespiratory variables were recorded before and at regular intervals for 60 minutes after drug administration. RESULTS: Bradycardia developed in all cats that were given romifidine or xylazine. No other significant differences in physiologic parameters were observed from baseline values or between treatments. Increasing the dose of romifidine did not result in increased sedation or muscle relaxation. Cats given xylazine showed higher sedation and muscle relaxation scores over time. Analgesia scores were significantly higher after administration of romifidine (400 microg kg(-1)) and xylazine (1 mg kg(-1)) than after romifidine at 100 or 200 microg kg(-1). Duration of lateral recumbency was not significantly different between treatments; however, cats took longer to recover after administration of 400 micro g kg(-1) romifidine. CONCLUSIONS AND CLINICAL RELEVANCE: Bradycardia is the most important adverse effect after IM administration of romifidine at doses ranging from 100 to 400 microg kg(-1) or 1 mg kg(-1) of xylazine in cats. The sedative effects of romifidine at 200 microg kg(-1) are comparable to those of 1 mg kg(-1) of xylazine, although muscle relaxation and analgesia were significantly less with romifidine than with xylazine.  相似文献   

Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells     

Flávio de Oliveira Silva  Priscila das Neves Santos  Evellyne de Oliveira Figueirôa  Cristiane Moutinho Lagos de Melo  Juliana Kelle de Andrade Lemoine Neves  Francisco Vassiliepe Sousa Arruda  João Batista Cajazeiras  Kyria Santiago do Nascimento  Edson Holanda Teixeira  Benildo Sousa Cavada  Ana Lúcia Figueiredo Porto  Valéria Rêgo Alves Pereira 《Research in veterinary science》2014

Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.  相似文献   

Notes on the gross anatomy of the heart of the broad-snouted caiman,Caiman latirostris (Daudin, 1802)     

Mariana de Oliveira Lima  Yhuri Cardoso Nóbrega  Marcelo Renan de Deus Santos  Louisiane de Carvalho Nunes  Rodrigo Giesta Figueiredo  Maria Aparecida da Silva 《Anatomia, histologia, embryologia》2021,50(2):350-359

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Application of mixed models for evaluating stability and adaptability of maize using unbalanced data     

André Gradowski de Figueiredo  Renzo Garcia Von Pinho  Heyder Diniz Silva  Marcio Balestre 《Euphytica》2015,202(3):393-409

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Virulence of different isolates of Aeromonas hydrophila in channel catfish     

J. De Figueiredo  J.A. Plumb 《Aquaculture (Amsterdam, Netherlands)》1977,11(4):349-354

The virulence of nine Aeromonas hydrophila isolates from diseased fish, diseased Macrobrachium (freshwater shrimp) and from pond water was determined in channel catfish (Ictalurus punctatus) fingerlings. According to the lethal dose ? 50% end point (LD₅₀) of each isolate, the water organisms were significantly (P < 0.05) less virulent than the isolates from diseased fish. All isolates were biochemically similar.  相似文献