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31.
以 1 7β-雌二醇 -黄素腺嘌呤二核苷酸结合物为标记物 ,以葡萄糖氧化酶为全酶 ,建立了 1 7β-雌二醇脱辅基酶再激活免疫测定系统 (1 7β- E2 - ARIS) ,完成了两条标准曲线的制作 ,通过 logit代换法对两条标准曲线进行了直线回归。两条标准曲线的拟合度 (r)分别为0 .996 7* * 和 0 .9991 * * ,灵敏度分别为 1 1 .5 4pg· m L- 1 (2 . 31 pg·孔 - 1 )和 1 4.88pg·m L- 1 (2 . 98pg·孔 - 1 ) ,平均变异系数(CV% )分别为 4.6 4%和 9.34% ,测定范围为 31 .2 5~ 1 0 0 0 pg·m L- 1 。 相似文献
32.
R J Slappendel I van der Gaag J J van Nes T S van den Ingh R P Happé 《The Veterinary quarterly》1991,13(1):30-40
A newly recognised disease, which we have given the provisional name of familial stomatocytosis-hypertrophic gastritis (FSHG), is described in two families of dogs of the Drentse partrijshond breed. The affected dogs consisted of 3 females and 5 males, 3 to 19 (mean 9.5) months of age at admission. The main clinical problems were diarrhoea, icterus, and ataxia and paresis of the pelvic limbs. Laboratory evaluation revealed abnormal red cell shape (stomatocytosis), increased osmotic fragility, haemolytic anaemia, and increased liver enzymes and serum bilirubin. Gastroscopic and histopathologic examination of the gastric mucosa revealed hypertrophic gastritis resembling Ménétrier's disease in man. Histologic findings in the liver were suggestive of progressive liver disease. Cysts were found in the kidneys of the five oldest patients. Electroneurography in 2 dogs revealed polyneuropathy. In the parents of 2 patients (sister and brother), there were no clinical or laboratory abnormalities. An autosomal recessive hereditary defect of lipid metabolism is suspected. 相似文献
33.
Total creatine kinase (CK) and CK-MM isoforms were determined in plasma and longissimus dorsi muscle extracts from normal pigs. Based on their total CK activity, the pigs were divided into two groups. Pigs of group 1 (n=16) had a mean plasma total CK of 298±16 U/L and the distribution of the CK-MM isoforms was 65.7±2.5% CK-MM3, 18.9±1.6% CK-MM2 and 15.3±1.5% CK-MM. In group 2 (n=18; 826±75 U/L total CK) four isoforms were observed: 3.1±0.9% CK-MM, 67.9±3.0% CK-MM3, 21.5±2.3% CK-MM2 and 7.5±1.3% CK-MM1. The differences between the two groups of pigs were significant (p<0.001) for CK-MM1 and the presence of CK-MM. Four CK-MM isoforms were also detected in longissimus dorsi muscle homogenates: 45.6±8.1% CK-MM, 32.6±11.7% CK-MM3, 16.6±2.3% CK-MM2 and 5.1±2.8% CK-MM1. The release of CK-MM isoforms from muscle into plasma seems to be unrelated to the concentration of these isoforms in striated muscle. 相似文献
34.
Comparative infectivity for axenic and specific-pathogen-free chickens of O2 Escherichia coli strains with or without virulence factors 总被引:1,自引:0,他引:1
Adhesion to epithelial respiratory cells, iron acquisition, and production of K1 polysaccharide capsules have been proposed as potential virulence factors of avian Escherichia coli. These factors were studied by inoculating groups of axenic or specific-pathogen-free (SPF) chickens intratracheally with O2 E. coli strains after previous challenge with a wild strain of infectious bronchitis virus (IBV). In all experiments, the association between IBV and an E. coli strain endowed with the three virulence factors previously mentioned resulted in the most severe pathological effects, as measured by mortality, weight gains, lesions, and reisolation of E. coli from internal organs. An E. coli strain devoid of virulence factors was able only to induce mild pathological effects restricted to the respiratory tract when combined with IBV. Both E. coli strains were more invasive in axenic chickens than in SPF chickens. These results confirm the probable involvement of the three factors studied in the pathogenic properties of avian E. coli. This model can be used to assess the role of virulence factors, by comparing pairs of positive and negative isogenic strains. 相似文献
35.
Fr. 《Journal of pest science》1942,18(10):120
Ohne Zusammenfassung 相似文献
36.
Fr. 《Journal of pest science》1942,18(12):141-142
Ohne Zusammenfassung 相似文献
37.
38.
Fr. 《Journal of pest science》1948,21(4):64
Ohne Zusammenfassung 相似文献
39.
Fr. 《Journal of pest science》1948,21(4):64-64
40.
Fr. 《Journal of pest science》1948,21(2):29-29
Ohne Zusammenfassung 相似文献