Effects of nucleo-cytoplasmic interactions on leaf volatile compounds from citrus somatic diploid hybrids     

Fanciullino AL  Gancel AL  Froelicher Y  Luro F  Ollitrault P  Brillouet JM 《Journal of agricultural and food chemistry》2005,53(11):4517-4523

Three diploid citrus somatic hybrids (cybrids) were produced by fusions combining nucellar callus-derived protoplasts of Willow Leaf mandarin (Citrus deliciosa Ten.) and Commune clementine (Citrus clementina Hort. ex Tan.) with, respectively, leaf protoplasts of Eureka lemon [Citrus limon (L.) Burm.] and Marumi kumquat [Fortunella japonica (Thunb.) Swing.] and leaf protoplasts of Marumi kumquat. Ploidy and origins of the nuclear, chloroplastic, and mitochondrial genomes were investigated by flow cytometry and nuclear and cytoplasmic simple sequence repeat analyses. Volatile compounds were extracted from the leaves of the three cybrids by a pentane/ether (1:1) mixture, analyzed by GC-MS, and compared to those of their parents. The cybrids were found to be very close to their nucleus-giving parent, suggesting that the main information for volatile compounds biosynthesis is contained in the nucleus. However, nucleo-cytoplasmic interactions occurred: the (mandarin + lemon) cybrid, possessing nucleus and chloroplasts of lemon and mitochondria from mandarin, synthesizes more monoterpene alcohols and esters than its nucleus-giving parent; the (clementine + kumquat) cybrid, possessing nucleus from kumquat and organelles from mandarin, synthesizes more monoterpene and sesquiterpene hydrocarbons and sesquiterpene alcohols than its nucleus-giving parent.  相似文献   

番茄形态结构模型参数的多目标拟合估算方法研究   总被引：1，自引：1，他引：1       下载免费PDF全文

董乔雪  王一鸣  Jean Francois Barczi  贺超兴 《农业工程学报》2006,22(2):1-5

对番茄生长模型参数进行准确估算是模型得以验证和面向应用的前提条件。基于番茄拓扑结构，采用非线性最小二乘优化方法，通过对植株形态数据的多目标拟合，实现模型参数的最优估计和校准。非线性最小二乘法的权值根据目标数据自动调整，使得不同量纲和不同数值范围的观测数据可以在同一水平上进行，提高了计算的精度和稳定性；另一方面用差商近似代替导数加快了算法的收敛速度。通过应用拟合优度检验，得出模型输出对观测值的拟合程度较好，说明模型合理，参数可靠。实践表明该估算方法是对模型进行验证的一个行之有效的方法。  相似文献   

Long range transport and deposition of mineral matter as a source for base cations     

A. Semb  J. E. Hanssen  F. Francois  W. Maenhaut  J. M. Pacyna 《Water, air, and soil pollution》1995,85(4):1933-1940

Mineral dust in the atmosphere is generally alkaline, and is a source of base cations in precipitation. Annual emissions of particles from large combustion plants and industrial processes in Europe is of the order of 24 million tonnes, and the calcium content may be as high as 1.4 million tonnes. Emissions from diffuse sources such as agricultural activities, construction and quarrying are much less well known. Emissions of dust from the Sahara have been estimated to more than 200 tonnes, with a calcium content of 3–5%. In northern Europe, airborne concentrations of calcium and the concentration of calcium in precipitation are generally consistent with the anthropogenic emissions and their regional distribution. Transport of dust from Sahara is a major source of base cations in precipitation around the Mediterranean Sea, but the influence diminishes further north. The concentration of calcium in precipitation decreases from south to north and from east to west in Europe. Dry deposition of alkaline particles is not well documented, but may be at least as high as the input by precipitation in regions where there are large emissions. There are still large uncertainty gaps with respect to emissions, transport and deposition of calcium-containing particles.  相似文献   

The Israeli-Palestinian Science Organization     

Wiesel T  Agre P  Arrow KJ  Atiyah M  Brézin E  Charfi FF  Cohen-Tanoudji C  Daar A  Jacob F  Kahneman D  Lee YT  Nicolaisen I  Nusseibeh S  Reuter H  Shoham Y  Sulston J  Walzer M  Yaari M 《Science (New York, N.Y.)》2007,315(5808):39

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An investigation into human pandemic influenza virus (H1N1) 2009 on an Alberta swine farm     

Krista J. Howden  Egan J. Brockhoff  Francois D. Caya  Laura J. McLeod  Martin Lavoie  Joan D. Ing  Janet M. Bystrom  Soren Alexandersen  John M. Pasick  Yohannes Berhane  Margaret E. Morrison  Julia M. Keenliside  Sonja Laurendeau  Elizabeth B. Rohonczy 《The Canadian veterinary journal. La revue veterinaire canadienne》2009,50(11):1153-1161

On May 2, 2009 the Canadian Food Inspection Agency notified the World Organization for Animal Health that an emerging novel influenza A virus (pandemic H1N1 2009) had been confirmed on a swine farm in Alberta. Over a 4-week period pigs in this farrow-to-finish operation were clinically affected by respiratory disease consistent with an influenza A virus infection and the presence of active viral infection was confirmed in all production areas by real-time polymerase chain reaction (RT-PCR). Despite clinical recovery of animals, there was reluctance by purchasers to receive animals from this operation due to concerns about the effect on both domestic and international markets. The owner decided to depopulate the entire herd due to impending welfare issues associated with overcrowding and economic concerns resulting from the inability to market these animals. Carcasses were rendered or composted and did not enter the human food or animal feed chain. The source of virus in this herd was determined to be an infected human. Zoonotic transmission to 2 individuals responding to the outbreak was suspected and recommendations to prevent occupational exposure are discussed.  相似文献   

Sensory threshold of 1,1,6-trimethyl-1,2-dihydronaphthalene (TDN) and concentrations in young Riesling and non-Riesling wines     

Sacks GL  Gates MJ  Ferry FX  Lavin EH  Kurtz AJ  Acree TE 《Journal of agricultural and food chemistry》2012,60(12):2998-3004

1,1,6-Trimethyl-1,2-dihydronaphthalene (TDN) is well-known to contribute "petrol" aromas to aged Riesling wines, but its prevalence and contribution to young Riesling or non-Riesling wines is not well understood. TDN concentrations were measured in 1-3-year-old varietal wines produced from Cabernet franc (n = 14 wines), Chardonnay (17), Cabernet Sauvignon (4), Gewurztraminer (4), Merlot (9), Pinot gris (6), Pinot noir (9), Riesling (28), or Sauvignon blanc (6). TDN concentrations in the Riesling wines, 6.4 ± 3.8 μg/L, were significantly higher than in all other varietals, 1.3 ± 0.8 μg/L. The odor detection thresholds for TDN were then determined in both model wine and a neutral white wine. Group sensory thresholds were found to be the same in both matrices, 2 μg/L, indicating little masking of TDN due to the odorants in the neutral white. The TDN sensory threshold was a factor of 10 below the previously reported odor threshold. On the basis of this revised threshold, 27 of 28 Riesling wines had suprathreshold TDN, whereas only 7 of 69 non-Riesling wines had suprathreshold TDN. The monoterpenes linalool and geraniol were also measured in the Riesling wines, and odor activity values (OAVs) were calculated for the monoterpenes and TDN. The OAV for TDN was higher than for the monoterpenes in 25 of 28 Riesling wines.  相似文献   

Visual markers for tomato derived from the anthocyanin biosynthetic pathway     

John I. Yoder  Francois Belzile  Yusen Tong  Andrew Goldsbrough 《Euphytica》1994,79(3):163-167

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Round Table Conclusions     

André Francois 《Plant foods for human nutrition (Dordrecht, Netherlands)》1983,32(3-4):469-471

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Detailed genetic analysis of feeding behaviour in Romane lambs and links with residual feed intake     

Christel Marie‐Etancelin  Dominique Francois  Jean‐Louis Weisbecker  Didier Marcon  Carole Moreno‐Romieux  Frdric Bouvier  Flavie Tortereau 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2019,136(3):174-182

Breeding strategies based on feed efficiency are now implemented in most animal species using residual feed intake (RFI) criteria. Although relevant, the correlated responses of feeding behaviour traits resulting from such selection on RFI are poorly documented. We report the estimated feeding behaviour at three time levels (visit, meal and day) and genetic parameters between the feeding behaviour traits and their links with RFI and its components. Feed intake, feeding duration at three time levels (per visit, meal and day), feeding rate, number of visits and time‐between‐visits were estimated for 951 Romane lambs fed via automatic concentrate feeders. Heritability estimates of feeding behaviour traits ranged from 0.19 to 0.54 with higher estimates for the day level than the visit level. Daily feed intake was not genetically linked to feed intake at the visit level, whereas feeding duration between visit and day levels was moderately correlated (R_g = +0.41 ± 0.12). RFI was not significantly correlated with feeding rate, but was positively linked to feed intake and feeding duration at the day level (+0.73 ± 0.09 and +0.41 ± 0.13, respectively) and negatively at the visit level (?0.33 ± 0.14 and ?0.22 ± 0.17, respectively). Selecting animals with lower RFI values might modify their feeding behaviour by increasing feed intake and feeding duration at the visit level but decrease the number of visits per day (+0.51 ± 0.14).  相似文献   

Antimicrobial Susceptibility of 41 Burkholderia mallei Isolates From Spontaneous Outbreaks of Equine Glanders in Punjab,Pakistan     

Abeera Naureen  Muhammad Saqib  Faqeer Muhammad  Rehan Ahmad  Ghulam Muhammad  Muhammad Nadeem Asi  Muhammad Hammad Hussain  Laeeq Akbar Lodhi  Muhammad Sarwar Khan  Francois M. Thibault 《Journal of Equine Veterinary Science》2010

Minimum inhibitory concentrations (MICs) of 20 antimicrobial agents for 41 isolates of Burkholderia mallei from natural outbreaks of equine glanders were determined by agar dilution. All isolates were intrinsically resistant to ampicillin (MIC₉₀ ≥128). Resistance to other antimicrobials was as follows: 95.1% to amoxicillin and cephradine, 85.4% to cefuroxime and norfloxacin, 68.3% to ceftizoxime and ceftriaxone, 61.1% to ceftiofur, 58.5% to oxytetracycline, 41.5% to ciprofloxacin, 58.5% to roxithromycin, 17.1% cefotaxime, and 12.2% clarithromycin. Overall resistance patterns revealed that 17% of isolates were simultaneously resistant to amoxicillin, cephradine, cefuroxime, ceftizoxime, ceftriaxone and norfloxacin. None of the isolates were resistant to amoxicillin-clavulanic acid, doxycycline, chloramphenicol, gentamicin or trimethoprim-sulphadiazine. Mode MICs for these antimicrobials were 2, 1, 8, 4 and 1 μg/ml, respectively. A majority of the isolates (∼ 94%) were susceptible to both enrofloxacin and ofloxacin. These data provide an updated perspective on susceptibility profiles of current strains of B. mallei in an endemic area.  相似文献