日本百脉根LjbHLH34基因克隆及耐旱功能鉴定     

刘福  陈诚  张凯旋  周美亮  张新全 《草业学报》2023,32(1):178-191

干旱是影响植物生长发育的重要环境因素。本研究分析了日本百脉根抗旱相关基因LjbHLH34的耐旱功能，初步解析其响应干旱胁迫的分子机制，以期为百脉根抗旱分子育种提供理论基础。本研究克隆得到的LjbHLH34基因大小为711 bp、编码236个氨基酸，属bHLH转录因子家族成员。系统进化树分析显示，LjbHLH34蛋白与拟南芥bHLHⅣ亚家族中AtbHLH34和AtbHLH104亲缘关系较近。实时荧光定量分析表明LjbHLH34在日本百脉根的根中表达量最高，叶中次之，茎中最少，暗示其在日本百脉根多个组织中发挥作用；同时LjbHLH34基因也受聚乙二醇(PEG)和脱落酸(ABA)诱导表达。在酵母中检测发现LjbHLH34具有转录激活活性；亚细胞定位试验表明LjbHLH34蛋白定位于细胞核中。将LjbHLH34基因转入拟南芥获得过表达株系。在200 mmol·L^-1甘露醇胁迫下，LjbHLH34转基因拟南芥的根长明显长于野生型。干旱处理后，野生型拟南芥比转基因拟南芥萎蔫程度更加明显，而转基因株系的相对含水量和超氧化物歧化酶(SOD)活性显著高于野生型，丙二醛(MDA)积累...  相似文献   

安徽省极端降水事件的时空分布特征及其对农业生产的动态影响分析     

付伟  司红君  程智  刘蕾 《中国农学通报》2017,33(15):117-123

为了减少极端降水事件给农业生产带来的损失,使农业生产能够主动应对极端降水事件变化。基于安徽省17个城市逐日降水数据,使用百分位法计算了1965—2014年安徽省年极端降水事件,在此基础上使用线性趋势和EOF方法对其时空分布特征进行分析,并以计量经济学的结构向量自回归模型为基础,将1989—2013年的极端降水事件作为1个因子与农业总产值、国内生产总值、农业机械总动力构建了新的气候-经济模型,使用脉冲响应函数和方差分解定量分析了极端降水事件对安徽省农业生产的动态影响。结果表明:近50年,安徽省极端降水事件平均11.59次/a,无显著年际变化趋势。区域分布基本呈南多北少的特征,各城市也均无显著的年际变化趋势。EOF分析显示,安徽省极端降水事件有2种典型分布特征:第1种为全省变化一致,变化程度江淮之间多,南北少,变化中心位于江淮之间东部;第2种为南部和北部变化相反,变化的中心分别位于皖南山区和沿淮淮北中部。2种分布在时间上均以震荡变化为主,近5年全省分别表现出一致偏多和南多北少的分布特征。极端降水事件对农业生产总体为正向的影响,影响程度约为0.01%,但贡献比例比较低。  相似文献   

“中国四库学高层论坛”会议综述     

刘艳伟  拓夫 《湖南农业大学学报(自然科学版)》2016,(6):38-40

2016年6月5-6日，由湖南大学岳麓书院主办的“中国四库学高层论坛”在岳麓书院举行，会议共收到论文45篇，与会专家学者围绕四库学研究的历史、《四库全书》的编纂、《四库总目》与学术、《四库总目》的考证与纠谬补缺及四库学的问题作了深入讨论。  相似文献   

四家动物园观赏肉食动物消化道寄生虫感染情况调查     

王宇  邹希明  张丽冰  付雪  宿欣  高远  王春仁 《黑龙江八一农垦大学学报》2017,29(1)

为了解哈尔滨北方森林动物园、佳木斯水源山公园、齐齐哈尔龙沙动植物园和鸡西动物园观赏肉食动物寄生蠕虫的感染情况,于2015年3~6月采用饱和盐水漂浮法和水洗沉淀法对四家动物园的5科94只观赏肉食动物的粪便进行了虫卵检查,结果有18只动物检出虫卵,阳性率为19.2%(18/94),蛔虫卵为常见虫种。本调查为四家动物园肉食动物消化道寄生虫病防治提供了科学依据。  相似文献   

Sulfadiazine pharmacokinetics in grass carp (Ctenopharyngodon idellus) receiving oral and intravenous administrations     

Ning Xu  Yu Fu  Fang Chen  Yongtao Liu  Jing Dong  Yibin Yang  Shun Zhou  Qiuhong Yang  Xiaohui Ai 《Journal of veterinary pharmacology and therapeutics》2021,44(1):86-92

This study aimed to examine the bioavailability (BA) and pharmacokinetic (PK) characteristics of sulfadiazine (SDZ) in grass carp (Ctenopharyngodon idellus) after oral and intravenous administrations. Blood samples were collected at predetermined time points of 0.083, 0.17, 0.5, 1, 2, 4, 8, 16, 24, 48, 72, and 96 hr (n = 6). The samples were extracted and purified by organic reagents and determined by the ultra‐performance liquid chromatography. The software named 3P97 was used to calculate relevant PK parameters. The results demonstrated that the concentration–time profile of SDZ was best described by a one‐compartmental open model with first‐order absorption after a single oral dose. The main PK parameters of the absorption rate constant (K_α), the absorption half‐life (t_{1/2 Kα}), the elimination rate constant (K_e), the elimination half‐life (t_1/2Ke), and the area under concentration–time profile (AUC_0‐∞) were 0.3 1/h, 2.29 hr, 0.039 1/h, 17.64 hr, and 855.78 mg.h/L, respectively. Following intravenous administration, the concentration–time curve fitted to a two‐compartmental open model without absorption. The primary PK parameters of the distribution rate constant (α), the elimination rate constant (β), the distribution half‐life (t_1/2α), the elimination half‐life (t_1/2β), the apparent distribution volume (V_SS), the total clearance (CL), and AUC_0‐∞ were 9.62 1/hr, 0.039 1/hr, 0.072 hr, 17.71 hr, 0.33 L/kg, 0.013 L h^?1 kg^?1, and 386.23 mg.h/L, respectively. Finally, the BA was calculated to be 22.16%. Overall, this study will provide some fundamental information on PK properties in the development of a new formulation SDZ in the future and is partially beneficial for the appropriate usage of SDZ in aquaculture.  相似文献   

Study of microRNA Expression Profile in Different Regions of Ram Epididymis     

Cuiling Wu  Chunxin Wang  Bo Zhai  Yunhui Zhao  Zhuo Zhao  Zhiyu Yuan  Mingxin Zhang  Kechuan Tian  Xuefeng Fu 《Reproduction in domestic animals》2021,56(9):1209-1219

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Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance,immune response and intestinal barrier function in weaned piglets     

Runqi Fu  Chan Liang  Daiwen Chen  Hui Yan  Gang Tian  Ping Zheng  Jun He  Jie Yu  Xiangbing Mao  Zhiqing Huang  Yuheng Luo  Junqiu Luo  Bing Yu 《Journal of animal physiology and animal nutrition》2021,105(5):898-907

The present study investigated the effects of Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function of weaned piglets. Twenty-four weaned piglets with an average body weight (BW) of 6.89 ± 0.15 kg were divided into four diets for 28 days. The treatments were basal diet (control), basal diet supplemented with antibiotic (20 mg/kg colistin sulphate and 40 mg/kg bacitracin zinc, AT), probiotics (400 mg/kg Bacillus coagulans ≥5 × 109 CFU/g, BC) or yeast hydrolysate (5000 mg/kg yeast hydrolysate, YH). Average daily gain (ADG) and average daily feed intake (ADFI) were improved by AT and YH diets (p < 0.05), while BC diet only increased ADG (p < 0.05). The complement 3 (C3), lysozyme (LZM) and tumour necrosis factor-α (TNF-α) concentrations in serum were increased in BC diet (p < 0.05). Feeding AT and YH caused the increase of jejunal villus height (p < 0.05), and a higher ratio of villus height/crypt depth was observed in AT, BC and YH groups (p < 0.05). The mRNA expression of zonula occludens-1 (ZO-1) in jejunal mucosa was up-regulated by AT, BC and YH diets (p < 0.05). Dietary AT, BC or YH inclusion decreased the interleukin-1β (IL-1β) concentration and TNF-α mRNA expression (p < 0.05), and YH supplementation even down-regulated toll-like receptor 4 (TLR4) and CD14 expressions (p < 0.05). In summary, the dietary administration of BC or YH both improves growth performance through promoting the intestinal barrier function, indicating both of them can serve as potential alternatives to antibiotics growth promoters for the piglet production.  相似文献   

青藏高原典型高寒荒漠生长季蒸散及水分消耗特征研究     

孙树娇  周秉荣  周华坤  王秀英  权晨  李甫  陈奇 《草地学报》2021,29(z1):137-145

为探究青藏高原典型高寒荒漠下垫面水分消耗规律,以2019年青藏高原典型高寒荒漠地区沱沱河站监测的涡动数据和微气象数据为基础,分析不同时间尺度蒸散值的变化特征和下垫面水分消耗特征,研究结果表明：小时平均蒸散发量最大值出现在14—15时,达0.46 mm·h^-1;日蒸散值最大值出现在7月7日,为8.58 mm·d^-1,最小值出现在4月2日,为0.30 mm·d^-1;整个生长季蒸散值呈先增加后减小趋势,其中7月份最大,为120.68 mm,4月份最小,为64.80 mm;生长季总蒸散发量为581.15 mm,平均每天蒸散4.01 mm。整个生长季累计降水量为235.70 mm,蒸散发量与降水量（Precipitation,Pr）的差值（The difference between ET and Precipitation,IETP）显示,2019年青藏高原典型高寒荒漠植被生长季（4—8月）水汽交换以下垫面水分消耗为主。  相似文献   

射频识别技术在畜禽养殖中的应用与展望     

廖超  程金花  任守文  李碧侠  付言峰  王学敏  徐小波 《猪业科学》2021,38(11):92-95

随着畜禽规模养殖的发展,高度集约化养殖模式将影响畜禽的健康状况、疫病防控和福利水平。一种无接触、无应激的射频识别（Radio Frequency Identifi cation,RFID）技术应运而生,该技术可以在无损状态下采集畜禽的行为、生理生化和养殖环境指标,从而为评估集约化养殖的效果和影响提供基础数据。文章讨论了三种常见类型的RFID系统在畜禽养殖中的应用,系统地比较了各类系统的优缺点及适宜应用场景,进一步提出RFID在畜禽养殖中的未来应用方向,以期为深化畜禽信息采集的研究提供参考。  相似文献   

Effects of Monoglycerides of Varying Fatty Acid Chain Length and Mixtures Thereof on Sponge‐and‐Dough Breadmaking Quality     

Kazuki Hattori  Brigitte Dupuis  Bin Xiao Fu  Nancy M. Edwards 《Cereal Chemistry》2015,92(5):481-486

Monoglycerides are widely used in the baking industry because of their antistaling effects, mainly suppressing crumb firming. Commercial monoglycerides are normally prepared from hydrogenated fats, with stearate being the most common fatty acid. In a previous study, monoglycerides such as monopalmitate (C16) and monostearate (C18) had positive effects on Canadian short process bread but no improvements on sponge‐and‐dough process (SDP) bread. The objective of this study was to investigate the effects of saturated monoglycerides of varying fatty acid chain length (C14–C22) on SDP breadmaking quality by using volume judgment, crumb image analysis, and texture measurements. Higher levels (1.00–1.50%) of all monoglycerides (C14, C16, and C18) significantly (P < 0.05) increased loaf volume and cell diameter. The larger cell diameter with increasing levels of these monoglycerides may have resulted from softer, more extensible dough handling properties and greater gas cell stability during baking. Addition of C16 and C18 caused the largest increase in crumb softness with increasing monoglyceride levels but showed relatively low resilience, which might be related to larger loaf volume (i.e., lower density of bread). However, addition of blended monoglycerides C14+C16 increased crumb softness and loaf volume while partially retaining resilience. Each monoglyceride had a different function in breadmaking quality and somewhat positive effects on SDP.  相似文献