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61.
The aim of this study was to determine whether a single dose of an inactivated bluetongue virus serotype 8 (BTV-8) vaccine altered semen quality in rams. Twenty sexually mature rams were assigned to three experimental groups: two groups of four animals were vaccinated and a third group of four animals was unvaccinated. The first group included rams with a history of natural BTV-8 infection in 2007 and the second and third groups included BTV-8 na?ve rams. Semen was collected prior to vaccination and for 4months post-vaccination. There were no significant differences in semen quality traits, including motility and concentration of spermatozoa, and percentages of living, normal dead and abnormal dead spermatozoa, between vaccinated and unvaccinated groups, or over time (P>0.05). The BTV-8 vaccine tested in this study did not appear to have any adverse effect on semen quality in rams.  相似文献   
62.
The coffee leaf-miner (CLM) (Leucoptera coffeella Guérin-Mèneville; Lepidoptera: Lyonetiidae), the main pest of coffee plants, occurs widely throughout the Neotropics where it has a significant, negative economic and quantitative impact on coffee production. This study was conducted in a rubber tree/coffee plant interface that was influenced by the trees to a varying degrees depending on the location of the coffee plants, i.e. from beneath the rubber trees, extending through a range of distances from the edge of the tree plantation to end in a coffee monocrop field. The most severe damage inflicted on coffee plants by the CLM (number of mined leaves) from April, which marks the start of the water deficit period, until September 2003 was in the zone close to the rubber trees, whereas the damage inflicted on plants in the monocropped field was comparable to that on coffee plants grown directly beneath the rubber trees, which received about 25–40 % of the available irradiance (Ir—available irradiation at a certain position divided by the irradiation received in full sunlight, i.e. in the monocrop). From May until July damage caused by the CLM nearly doubled in each month. In midwinter (July), the damage decreased perceptibly from the tree edge toward the open field. From September onward, with the rising air temperatures CLM damage in the coffee monocrop started to increase. Based on these results, we conclude that coffee plants grown in the full sun incurred the most damage only at the end of winter, with warming air temperatures. Coffee plants grown in shadier locations (25–40 % Ir) were less damaged by the CLM, although a higher proportion of their leaves were mined. The rubber trees probably acted as a shelter during the cold autumn and winter seasons, leading to greater CLM damage over a distance outside the rubber tree plantation that was about equal to the height of the trees. Future studies should attempt to relate leaf hydric potential to pest attack in field conditions. More rigorous measurements of shade conditions could improve our understanding of the relationship of this factor to CLM attack.  相似文献   
63.
64.
This study used multivariate statistics to identify clusters of animals with similar expected progeny difference (EPD) and also identify leading traits that discriminate between bulls. Various linear selection indices based on specific selection criteria were proposed. Records were collected from 880 young Nelore bulls submitted to performance testing in central Brazil between 2001 and 2012. Pre-weaning average daily gain and weights at 210 days with direct and maternal effects were used in the analysis, in addition to post-weaning weight, scrotal circumference at 365 and 450 days, carcass finish and rib eye area. EPDs were classified into three groups, and the EPD means of two of these groups stood out and were considered important based on principal component analysis that associated higher values of direct EPD of weights, average daily weight gain and scrotal circumference. The EPDs for weight at 210, 365 and 450 days, pre- and post-weaning daily gain and scrotal circumference at 365 and 450 days were major causes of variation. Each linear selection index proposed (SI1, SI2, SI3, SI4 and SI5) defined a specific approach meaning that a different selection index should be used depending on breeding goals and selection criteria.  相似文献   
65.
The aim of this study was to determine the effects of ultraviolet radiation (UVR) on bioaccumulation of UV‐absorbing compounds acquired through the diet, in larvae and postlarvae of Pleoticus muelleri, and to assess tissue antioxidant activity, survival, and development. Mysis stage I were exposed to two artificial radiation treatments: M‐PAR (photosynthetically active radiation, range = 400–700 nm) and M‐PAR + UVR (280–700 nm). The experimental larvae received a mixed dietary treatment of Artemia persimilis and the microalga Pavlova lutheri, reared under two radiation regimes: PAR (D‐PAR) and PAR + UVR (D‐PAR + UVR). Shrimp from all treatments reached 8 d postlarval stage (PL8), except those under M‐PAR + UVR treatment fed the D‐PAR‐cultured algae, which had 0% survival. Larvae in M‐PAR + UVR and M‐PAR treatments fed with D‐PAR + UVR diet presented the highest survival rates (70 and 75%, respectively), with 37 and 41% increase in PL size. UV‐absorbing compounds were detected in microalgae and PL subject to PAR + UVR treatments. Antioxidant activity, quantified by measuring the free‐radical 1,1‐diphenyl‐2‐picrylhydrazyl in homogenates of PL8, decayed drastically under radiation treatment M‐PAR + UVR fed with algae of the D‐PAR + UVR treatment. It is concluded that the bioaccumulation of UV‐absorbing compounds and the highest antioxidant activity in PL could improve the biochemical and photophysiological responses of shrimp under UVR stress.  相似文献   
66.
Tropical Animal Health and Production - To reduce costs, lactating cows are often fed a higher proportion of roughage and alternative feeds; however, such protocols may not lead to optimal milk...  相似文献   
67.
When first described in 1946, bovine viral diarrhea (BVD) was characterized as an acute transmissible disease associated with severe leucopenia, high fever, depression, diarrhea, gastrointestinal erosions, and hemorrhages. Recently the severe acute form has been related only to some hypervirulent BVDV-2 strains. This article reports the detection of BVDV-1b associated with an acute and fatal outbreak of BVD in a Brazilian beef cattle herd. Depression, anorexia, watery diarrhea, sialorrhea, and weakness were observed in six steers. One of these animals was evaluated for laboratorial, clinical, and pathological alterations. Laboratory findings were non-specific; clinically, the animal was weak, with dehydration and erosive oral lesions. Pathological alterations were predominant at the tongue, esophagus, and rumen. A RT-PCR assay using primers to partially amplify the 5′ untranslated region (5′UTR) of the BVDV genome was performed and identified BVDV in all clinical samples analyzed. Phylogenetic analysis of BVDV derived from lymph node revealed that this strain was clustered within the BVDV subtype 1b. This differentiating was only possible to be performed by molecular characterization since both clinical presentation and pathologic findings were similar to BVDV-2 infection.  相似文献   
68.
A bioeconomic simulation model for Lates calcarifer predicted that a strategy involving crossing current generation males with previous generation females would be a practical, effective and profitable way of dealing with protandry when batch rearing for selective breeding to improve the growth rate. The strategy allowed earlier initialization and more frequent ongoing rounds of selection, and resulted in a 16–19% higher overall response, than an alternative where each generation's males were crossed with the same generation's females. The strategy also yielded the highest short‐term benefit–cost ratio (13:1 versus 7:1 after 8 years of selective breeding) and the highest short‐ and long‐term value for participants in a breeding cooperative (a net present value of AU$28 million and an internal rate of return of 144% over 10 years), due to higher yields per fixed costs of production per unit area and due to savings in feed costs per kilogram of production. Breeding facilities of scale producing 50 full‐sibling families per generation were found to be more profitable than those producing 100 families.  相似文献   
69.
By‐catch in marine fisheries, particularly those using pelagic and demersal longlines, is a major driver of declines in abundance of sharks and rays around the world. A wide variety of by‐catch reduction devices (BRDs), that is, modified gears designed to reduce incidental captures of a variety of marine species while maintaining target catch rates, have been proposed, but the extent to which BRDs actually reduce the risk of catching sharks and rays remains unclear. We performed a meta‐analysis of 27 publications that reported the capture of sharks and rays and, in some cases, of targeted teleosts in longline gear deployed with and without BRDs. The risk of shark and ray capture differed between types of BRDs, but only one BRD type, longlines raised off the bottom, reduced by‐catch significantly. Circle hooks did not reduce the risk of capturing sharks and rays but might improve discard survival and are inexpensive, which might make them effective in reducing the detrimental effects of longlining on these species. In addition to being generally ineffective, some devices, such as electropositive and magnetic repellents, are expensive and have inherent construction drawbacks that are likely to make them unsuitable for commercial use. Overall, most BRDs did not affect the likelihood of catching targeted teleosts, but a substantial number of studies did not adequately assess target catch. We identified two poorly studied classes of BRD gear (i.e. raised demersal longlines, and monofilament nylon leaders), which represent promising directions for future research.  相似文献   
70.
This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml.  相似文献   
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