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81.
Sajid Ali Marc Leconte Hidayatur Rahman Muhammad Shahab Saqib Pierre Gladieux Jérôme Enjalbert Claude de Vallavieille-Pope 《European journal of plant pathology / European Foundation for Plant Pathology》2014,140(2):275-290
Information on the pathogen virulence profile and diversity across locations is crucial for host germplasm improvement and deployment. The rapid acquisition of virulence to host resistance by the wheat yellow/stripe rust pathogen (Puccinia striiformis f.sp. tritici: PST), makes it crucial to know about its virulence and pathotype diversity. Recent studies have shown the plausible centre of origin of the pathogen in the Himalayan region, with Pakistan being the most ancestral to all other worldwide populations. To assess the status of virulence and pathotype diversity in the Himalayan region of Pakistan, a set of 127 PST infected wheat samples from eight locations were collected, multiplied and pathotyped using a set of 36 differential lines from the world set, European and Chinese sets, and 9 Avocet Yr isolines. Virulence (Vr) was recorded to 18 out of 24 tested yellow rust resistance (Yr) genes, while a total of 53 pathotypes were detected out of 127 isolates tested. Virulence was found to the resistance genes rarely deployed in Pakistan (Vr8) or even worldwide level (Vr5), while virulence to Vilmorin 23 (Yr3+) was absent in Pakistan, which is common in Europe. None of the pathotypes was dominant across all locations, however, no clear spatial structuring was observed for the studied locations. Our results suggested a high virulence and pathotype diversity in line with the previously proposed potential role of sexual recombination in the temporal maintenance of PST in the Himalayan region of Pakistan. This information should be useful in host resistance gene improvement and deployment. 相似文献
82.
GT Bleck MB Wheeler LB Hansen H Chester-Jones DJ Miller 《Reproduction in domestic animals》2009,44(2):241-247
It is believed that milk production is determined by the number and activity of mammary secretory cells. Secretory activity, as assessed by milk volume, depends on secretion of the major osmole in milk, lactose, which is produced by lactose synthase. The amount of either of the two proteins in lactose synthase may regulate milk production. The objective of this study was to determine whether the concentrations in milk of the two components of lactose synthase, α‐lactalbumin (α‐LA) and β1,4‐galactosyltransferase (B4GALT), were related to genetic background, stage of lactation, breed or parity of dairy cows. α‐Lactalbumin and B4GALT concentrations were measured by ELISA and by enzyme assays, respectively, from single milk samples. Two herds with a total of 279 cows were used in the analysis. One herd contained Ayrshire, Brown Swiss, Holstein and Jersey cows; the second herd contained two groups of cows; Holsteins selected for high milk production and Holsteins with 1960s genetics. The α‐LA concentration in milk was greater in Jerseys and Ayrshires than in Holsteins and Brown Swiss. However, no difference in α‐LA concentration was observed in milk from high and low genetic merit cows in the Minnesota herd or among different genetic backgrounds in the Illinois herd. β1,4‐Galactosyltransferase concentrations were similar for all groups that were analyzed. α‐Lactalbumin concentrations were positively correlated with milk protein concentration, milk fat concentration and lactose concentration. β1,4‐Galactosyltransferase concentration in milk exhibited a strong positive correlation with number of days in milk. Although the concentration of B4GALT increased as lactation progressed, the values did not show any correlation with persistency of lactation or late lactation milk production. In conclusion, this survey shows that the two components of lactose synthase are each correlated to protein concentration and individually correlated to the concentration of other milk components and stage of lactation. 相似文献
83.
84.
Eduardo Antônio Sanches Giovano Neumann César Pereira Rebechi de Toledo Robie Allan Bombardelli Pitágoras Augusto Piana Elizabeth Romagosa 《Aquaculture Research》2013,44(4):534-541
The aim of this work was to evaluate the spermatic parameters of jundia, Rhamdia quelen, semen stored for short periods under different temperatures. Fifteen males were used in a time factorial experimental design (13 × 3 × 3 × 3). The sperm was stored at: 15; 25 and 35°C and activated in water at: 15; 25 and 35°C each, respectively, within storage periods of: 0; 2; 4; 6; 8; 10; 12; 16; 20; 24; 32; 40 and 48 h after collection. The treatments were performed in triplicates and in sequential protocols every 50 h. The motility parameters were evaluated using Pearson's correlation analysis and the significant parameters (P < 0.05) were summarized using principal component analysis forming two predicted groups. Group 01 composed the following parameters: curvilinear velocity, wobble and linearity; group 02 comprised average path velocity, straight line velocity, beating cross frequency and motility rate. Effects (P < 0.05) of treatments were observed only in group 02, with interaction between time and temperature of exposure and between exposure time and water temperature. Superior results of spermatic parameters of group 02 were observed right after collection. However, the exposure and spermatic activation at 15°C assured significant results 48 h after collection when compared with 25 and 35°C. 相似文献
85.
This study evaluated the effect of dietary supplementation with l-tryptophan (L-TRP), a serotonin precursor, on the aggressiveness of juvenile matrinxã Brycon amazonicus. Fish were kept in individual aquaria for 7 days receiving the diets: D1 (control: 0.47% of TRP), D2 (0.94% of TRP), D3 (1.88% of TRP), and D4 (3.76% of TRP). After this, they were grouped with an intruder fish to establish a resident–intruder relationship during periods of 20 min. Blood cortisol, glucose, chloride, sodium and calcium; hemoglobin, hematocrit, red blood cell count and volume; liver glycogen and lipids were measured. Territoriality had significant effect on the aggressiveness of matrinxã (the residents were more aggressive than intruders, P < 0.001) and tryptophan significantly affected their behavior. Fish fed with the D2 diet presented a longer latency until the first attack (P = 0.0069) and bit the intruder fewer times (P = 0.0136) during the period of observation, compared to the control group. The frequency of bites and chases after the first attack was not affected by the dietary supplementation of TRP. Physiological variables were not significantly affected by the diet, except for a moderate increase in cortisol level in fish fed with D2 diet after the fight, indicating slight activation of the hypothalamus–pituitary–interrenal axis. The results show that juvenile matrinxã have aggressive and territorial behavior and that a diet containing 9.4 g TRP kg?1 alter their aggressiveness, without affecting the stress-related physiological parameters. 相似文献
86.
Fabieli Pelissari Anderson Cleiton José Marco Aurélio Leite Fontes Antônio César Batista Matos Wilson Vicente Souza Pereira José Marcio Rocha Faria 《New Forests》2018,49(1):143-158
Knowledge of seed desiccation tolerance is fundamental for conservation and use of forest species. The protocol used for classification of seed desiccation tolerance and storage is time consuming and many times limited by the lack of information about optimum conditions for seed germination and treatments to overcome seed dormancy. This study evaluated 66 Brazilian tree species aiming to correlate seed characteristics with desiccation tolerance. For this purpose, a model was established to explain the relationship of tegument/seed mass ratio (SCR), seed mass, and water content of embryo + endosperm with desiccation tolerance. The principal component analysis showed the establishment of two groups, indicating the interaction between desiccation tolerance and seed characteristics. Recalcitrant seeds are more often associated with the water content of embryo + endosperm and water content of tegument + endocarp, while orthodox seeds are more associated with SCR and number of seeds per kilogram. The classification found using the model proposed was significantly correlated with desiccation tolerance and storage, with 92% confidence for the analyzed species. Seeds morphological characteristics can be used for prediction of desiccation tolerance and storage behavior; however, the use of a model that combines more variables increases the chance of accurate classification. 相似文献
87.
Ingrid Seynave Alain Bailly Philippe Balandier Jean-Daniel Bontemps Priscilla Cailly Thomas Cordonnier Christine Deleuze Jean-François Dhôte Christian Ginisty François Lebourgeois Dominique Merzeau Eric Paillassa Sandrine Perret Claudine Richter Céline Meredieu 《Annals of Forest Science》2018,75(2):48
Key message
The diversity of forest management systems and the contrasted competition level treatments applied make the experimental networks of the GIS Coop, a nationwide testing program in the field of emerging forestry topics within the framework of the ongoing global changes.Context
To understand the dynamics of forest management systems and build adapted growth models for new forestry practices, long-term experiment networks remain more crucial than ever.Aims
Two principles are at the basis of the experimental design of the networks of the Scientific Interest Group Cooperative for data on forest tree and stand growth (GIS Coop): contrasted and extreme silvicultural treatments in diverse pedoclimatic contexts.Methods
Various forest management systems are under study: regular and even-aged stands of Douglas fir, sessile and pedunculate oaks, Maritime and Laricio pines, mixed stands of sessile oak, European silver fir, and Douglas fir combined with other species. Highly contrasted stand density regimes, from open growth to self-thinning, are formalized quantitatively.Results
One hundred and eighty-five sites representing a total of 1206 plots have been set up in the last 20 years, where trees are measured regularly (every 3 to 10 years). The major outputs of these networks for research and management are the calibration/validation of growth and yield models and the drawing up of forest management guides.Conclusion
The GIS Coop adapts its networks so that they can contribute to develop growth models that explicitly integrate pedoclimatic factors and thus also contribute to research on the sustainability of ecosystems under environmental and socio-economic changes.88.
Gerónimo Quiñonez-Barraza Dehai Zhao Héctor M. De Los Santos Posadas José J. Corral-Rivas 《Annals of Forest Science》2018,75(3):78
Key message
More accurate diameter at breast height (dbh)-growth models are needed for developing management tools for mixed-species forests in Mexico. Individual distance-dependent dbh growth models that quantify local neighborhood effects have been developed for four species groups in such forests. The performance of the models is improved by distinguishing between inter- and intraspecific group competitions.Context
The management of mixed-species forests in the northwest of Durango, Mexico, is mainly based on the selection method. Understanding the interspecific and intraspecific competition is critical to developing management tools for such mixed-species forests.Aims
An individual-based distance-dependent modeling approach was used to model the growth of dbh and to evaluate neighborhood effects for four species groups in Mexican mixed-species stands.Methods
Twenty-two species were classified into four groups: Pinus (seven species), other conifers (three species), other broadleaves (four species), and Quercus (eight species). Four methods were used to select neighboring trees and 12 competition indices (CIs) were calculated. Comparisons of the neighboring trees selection methods and CIs and tests of assumptions about neighborhood effects were conducted.Results
Intra-species-group competition significantly reduced diameter growth for all species groups, except for the Quercus group. The Pinus, other conifers, and Quercus groups had significant and negative neighborhood effects on the other broadleaves species group, and not vice versa. The Quercus group also had negative neighborhood effect on the Pinus and other conifers species groups, and not vice versa. The Pinus and other conifers species groups had negative neighborhood effects on each other. All fitted age-independent dbh growth models showed a good of fit to the data (adjusted coefficient of determination larger than 0.977).Conclusion
The growth models can be used to predict dbh growth for species groups and competition in mixed-species stand from Durango, Mexico.89.
L. Shen A.H. Estrada E. Côté M.A. Powell B. Winter K. Lamb 《Journal of Veterinary Cardiology》2017,19(2):144-152
Introduction
To determine the relationship between aortoseptal angle (AoSA) and the short- and long-term systolic pressure gradient (PG) reduction following combined cutting and high-pressure balloon valvuloplasty (CB/HPBV) in dogs with severe subaortic stenosis.Animals
Retrospective study of 22 client-owned dogs of various breeds with severe subaortic stenosis (mean left ventricular to aortic PG = 143 mmHg; range = 80–322 mmHg) that underwent CB/HPBV.Materials and methods
Initial angiographic and left apical and right-sided parasternal long-axis view echocardiographic video loops were used for measuring the angle between the plane of the interventricular septum and the longitudinal axis of the ascending aorta. The PG reduction ratio immediately after CB/HPBV and 6 and 12 months later were compared with AoSA.Results
Weak correlations were observed for all instances of PG reduction ratio and AoSA type. Significantly greater mean differences of PG reduction ratio were observed for angles >160° than for angles <160° at 24 h (>160° mean: 54.45, standard error [SE]: ±3.8; <160° mean: 39.88, SE: ±2.09), 6 months (>160° mean: 57.73, SE: ±10.9; <160° mean: 28.22, SE: ±3.42), and 12 months (>160° mean: 76.11, SE: ±17.5; <160° mean: 27.61, SE: ±6.44; p=0.003).Conclusions
Dogs with AoSA >160° on right-sided parasternal long-axis view echocardiograms responded with a greater PG reduction following CB/HPBV than did dogs with AoSA <160°. This suggests that AoSA is associated with long-term outcomes of CB/HPBV, and measurement could help in the evaluation of dogs that are candidates for CB/HPBV. 相似文献90.
Organogenesis and foetal haemodynamics during the normal gestation of healthy black‐rumped agoutis (Dasyprocta prymnolopha,Wagler, 1831) bred in captivity
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FCA Sousa GT Pessoa LS Moura JR Araújo RPS Rodrigues MAPS Barbosa AN Diniz AB Souza EG Silva LU Lucena MP Sanches OF Silva‐Filho PC Guerra JM Sousa WC Neves FR Alves 《Reproduction in domestic animals》2017,52(1):60-66
The objective of this study was to define the patterns of organogenesis and foetal haemodynamics during the normal gestation of healthy agoutis (Dasyprocta prymnolopha) kept in captivity. Thirty pregnant agoutis that ranged in size from small to medium and weighed between 2.5 and 3 kg underwent B‐mode and Doppler ultrasonography for the biometric evaluation of the foetal organs. The foetal aortic blood flow proved to be predominantly systolic, and the measured flow velocity was 78.89 ± 2.95 cm/s, with a maximum pressure gradient of 2.12 ± 0.27 mmHg. The liver was characterized by its large volume, occupying the entire cranial aspect of the abdominal cavity, and it was associated cranially with the diaphragm and caudally with the stomach. The flow velocity in the portal vein was estimated to equal 12.17 ± 2.37 cm/s, with a resistivity index of 0.82 ± 0.05. The gallbladder was centrally located and protruded cranially towards the diaphragm. The spleen was visualized as an elongated structure with tapered cranial and caudal extremities, and the foetal kidneys were visualized bilaterally in the retroperitoneal region, with the right kidney positioned slightly more cranially than the left. The morphological characterization and hemodynamic analysis of the foetal organs of black‐rumped agoutis via B‐mode and Doppler ultrasonography allow determination of the vascular network and of reference values for the blood flow required for perfusing the anatomical elements essential for maintaining the viability of foetuses at different gestational ages. 相似文献