Restriction Enzyme Analysis of Indian Isolates of Egg Drop Syndrome 1976 Virus Recovered from Chicken,Duck and Quail     

Senthilkumar N  Kataria JM  Koti M  Dhama K  Dash BB 《Veterinary research communications》2004,28(5):447-453

Egg drop syndrome 1976 (EDS-76) is caused by a haemagglutinating adenovirus belonging to group III of the genus Aviadenovirus in the family Adenoviridae. All isolates are serologically identical, but have been divided into three groups based on restriction endonuclease (RE) analysis. In this study the viral DNA of various Indian EDS-76 viral isolates (CEDS-A, CEDS-B, EDS-M, EDS-ML, EDS-1/AD/86, EDS-KC and QEDS) obtained from different avian species and different geographical regions were digested with restriction endonucleases viz., EcoRI, BamHI, HindIII and PstI. The results showed that one Indian isolate obtained from duck (DEDS-KC) was different from all other chicken and quail counterparts. All other isolates were identical to the reference viral strain BC-14, which belong to group I of EDS-76 viruses. The duck isolate EDS-KC could not be placed in any of the three groups reported earlier.  相似文献   

Sequence Analysis of an Indian Field Isolate of Infectious Bursal Disease Virus Shows Six Unique Amino Acid Changes in the VP1 Gene     

Bais MV  Kataria RS  Tiwari AK  Viswas KN  Reddy AV  Prasad N 《Veterinary research communications》2004,28(7):641-646

Veterinary Research Communications -  相似文献   

Differential detection of Newcastle disease virus strains by degenerate primers based RT-PCR   总被引：2，自引：0，他引：2  

Tiwari AK  Kataria RS  Nanthakumar T  Dash BB  Desai G 《Comparative immunology, microbiology and infectious diseases》2004,27(3):163-169

Degenerate primers based RT-PCR (previously described by [Avian Dis 26 (1997) 837]) has been used for the detection and differentiation of Newcastle disease (ND) viruses. Two sets of primers (A+B and A+C), with common forward primer and distinct reverse degenerate primers, designed from fusion protein gene encoding for cleavage site, could differentiate virulent and avirulent Newcastle disease viruses (NDV). Both sets of primers amplified "F" gene sequence of virulent (velogenic and mesogenic) viruses, whereas in avirulent strains, amplification was only with primer set A+C. Total 10 NDV isolates and two clinical samples including both known and unknown pathotypes, were checked. Based on amplification results 5 viruses were found to be virulent type and 6 as avirulent with one of the two clinical samples, earlier positive by RT-PCR using non-degenerate "F" gene specific primers was found negative in this study. The technique has been found to be a simple and quick for the detection and differentiation of virulent and avirulent NDV, which is important for control of the disease in the events of the outbreaks.  相似文献   

The Hydropericardium Syndrome in Poultry – A Current Scenario   总被引：2，自引：0，他引：2  

Balamurugan V  Kataria JM 《Veterinary research communications》2004,28(2):127-148

Inclusion-body hepatitis hydropericardium syndrome (IBH-HPS) is an important, recently emerged, disease of poultry, particularly of 3- to 6-week-old broiler chicks, characterized by its sudden onset, with high mortality ranging from 20% to 70%, typical hydropericardium and enlarged mottled and friable livers, with intranuclear inclusion bodies in the hepatocytes. The causative agent is a non-enveloped icosahedral fowl adenovirus (FAV) serotype 4, belonging to the Adenovirus genus of the family Adenoviridae, which can be propagated or cultivated in chicken embryo liver and kidney primary cell cultures. The transmission of disease occurs vertically and laterally by the oral-faecal route. The liver of infected birds shows necrotic foci and basophilic intranuclear inclusion bodies in the hepatocytes. The disease can be diagnosed from its gross and histopathological changes in the liver and by various serological tests, such as agar gel immunodiffusion, counterimmunoelectrophoresis, indirect haemagglutination, the fluorescent antibody technique, enzyme-linked immunosorbent assay and the polymerase chain reaction. The disease has been brought under control by the use of formalin-inactivated vaccines, prepared from infected liver homogenate, and of inactivated cell culture vaccines. The vaccines are effective in the face of natural outbreaks or experimental challenge and significantly reduce the mortality.  相似文献   

Implications of differences in macromolecular composition of stem fractions for processing of Scots pine     

Sara Johansson  Karin Carlqvist  Rashmi Kataria  Thomas Ulvcrona  Urban Bergsten  Mehrdad Arshadi  Mats Galbe  Gunnar Lidén 《Wood Science and Technology》2015,49(5):1037-1054

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Acaricide resistance in Rhipicephalus microplus collected from selected districts of Madhya Pradesh,Uttar Pradesh and Punjab states of India     

Sagar  Sharath V.  Saini  Kuldeep  Sharma  Anil Kumar  Kumar  Sachin  Kumar  Rinesh  Fular  Ashutosh  Shakya  Mukesh  Upadhaya  Deepak  Nagar  Gaurav  C.  Shanmuganath  Samanta  Subhamoy  Kumar  Suman  Ghosh  Srikant 《Tropical animal health and production》2020,52(2):611-618

Tropical Animal Health and Production - The isolates of Rhipicephalus microplus collected from Madhya Pradesh (MP), Punjab (PJB) and Uttar Pradesh (UP) states of India were characterized using...  相似文献   

Sequence analysis of Toll-like receptor genes 1-10 of goat (Capra hircus)     

Raja A  Vignesh AR  Mary BA  Tirumurugaan KG  Raj GD  Kataria R  Mishra BP  Kumanan K 《Veterinary immunology and immunopathology》2011,140(3-4):252-258

This study involved cloning and sequencing of the coding regions of all 10 Toll-like receptor (TLR) genes of goat. Goat TLR 1-10 gene sequences revealed a high degree of nucleotide identity with sheep and cattle sequences (>90%) and 75-85% with pig, mouse and human sequences. At the amino acid level, 85-99% similarity was observed with sheep and cattle and 60-85% with pig, mouse and human. TLR9c DNA of goat showed the highest amino acid identity to that of sheep (99%) while TLR8 cDNA showed the lowest identity of 88.7% to that of sheep. Variations were seen in the number of leucine rich repeats (LRRs) of goat TLRs as compared to other ruminant species with maximum differences in the TLR3 gene. Phylogenetic analysis through molecular evolution and genetic analysis (MEGA) software and multi dimensional scaling revealed a high degree of conservation of goat TLRs with those from other species. However when the TIR domain of all the TLRs were compared, goat TLR7 TIR alone showed a high divergence of 19.3 as compared to sheep sequences. This is the first report of the full-length cDNA sequences of all the 10 TLR genes of goats which would be a useful tool for the study of evolutionary lineages and for phylogenetic analysis.  相似文献   

Potential of ivermectin as an active ingredient of the attractive toxic sugar baits against the Indian malaria vectors Anopheles culicifacies and Anopheles stephensi     

Gaurav Kumar  Shweta Pasi  Chander Prakash Yadav  Jaspreet Kaur  Amit Sharma 《Pest management science》2023,79(1):474-480

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Soil loss hinders the restoration potential of tree plantations on highly eroded ravine slopes     

Kumar  Raj  Bhardwaj  A. K.  Rao  B. K.  Vishwakarma  A. K.  Kakade  Vijaysinha  Dinesh  D.  Singh  Gaurav  Kumar  Gopal  Pande  V. C.  Bhatnagar  P. R.  Bagdi  G. L. 《Journal of Soils and Sediments》2021,21(2):1232-1242

Journal of Soils and Sediments - Soil erosion and loss threatens vast tracts of agricultural and non-agricultural land, worldwide. High soil erosion severely affects establishment of vegetation via...  相似文献   

In response to HMOX1 microsatellite polymorphism by Cao et al     

Gaurav Sikri  Srinivasa Bhattachar 《The Forestry Chronicle》2018,29(2):278-279

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