Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822)     

Dharmendra Kumar Chaudhary  Neeraj Sood  Gaurav Rathore  Pravata Kumar Pradhan  Peyush Punia  Naresh Kumar Agarwal  Joy Krushna Jena 《Aquaculture Research》2014,45(2):299-311

A continuous cell line has been developed from thymus explants of Catla catla and the cells have been subcultured for 63 passages. The cells exhibited optimum growth at 30°C in L‐15 medium containing 15% foetal bovine serum. The cultured cells engulfed yeast cells and fluorescent latex beads. These cells produced reactive oxygen and nitrogen intermediates following stimulation with lipopolysaccharide and phorbol esters. The culture supernatant from the cultured cells had lysozyme activity and these cells demonstrated Fc receptors. Almost all the cells were positive for alpha‐naphthyl acetate esterase enzyme suggesting that the cells are of macrophage lineage and therefore, the cell line was designated as catla thymus macrophage (CTM) cell line. CTM cells formed aggregates around zoospores of Aphanomyces invadans, but were unable to inhibit the germination of spores. The karyotype analysis of CTM cells at 25th passage revealed a typical diploid model with 50 chromosomes per cell. Partial amplification, sequencing and alignment of fragments of two mitochondrial genes 16S rRNA and cytochrome c oxidase subunit 1 confirmed that the CTM cell line originated from C. catla. This cell line should be useful for studying the role of macrophages in differentiation and maturation of thymocytes and can be a source of macrophage‐specific enzymes and cytokines.  相似文献   

Induced resistance by oxidative shifts in pigeonpea (Cajanus cajan L.) following Helicoverpa armigera (Hübner) herbivory          下载免费PDF全文

Rimaljeet Kaur  Anil K Gupta  Gaurav K Taggar 《Pest management science》2015,71(5):770-782

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Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight     

Mamta Sharma  Suresh Pande  Abhishek Rathore 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(3):325-331

Ascochyta blight (AB, Ascochyta rabiei (Pass.) Lab.) is one of the most important foliar disease of chickpea (Cicer arietinum L.), globally. Chickpea is attacked by AB at any growth stage in cool and humid weather depending on the inoculum availability. However, the disease epidemics are most prominent during the flowering and podding growth stages. The main objective of this study was to determine the effect of growth stages of chickpea on the genetic resistance of AB and use this information in a resistance breeding program. Two susceptible and two moderately resistant chickpea cultivars were spray inoculated at seedling (GS1), post-seedling (GS2), vegetative (GS3), flowering (GS4) and podding (GS5) growth stages with A. rabiei conidial suspension under controlled environment conditions. Irrespective of crop cultivars the incubation period (IP) was shorter in GS1, GS4 and GS5 and was significantly extended in GS2 and GS3. Symptom development was delayed significantly in moderately resistant cultivars. The AB severity 10 days after inoculation ranged between 7 and 9 on susceptible cultivars and 3 and 5 on moderately resistant cultivars. Further the correlation coefficient of disease severity between GS1, GS4 and GS5 was highly significant (r = 0.95) indicating that, evaluation for resistance to AB can be done at GS 1 (seedling stage), and or GS4 (flowering stage) to GS5 (podding stage) growth stages of chickpea. This supports the evaluation for AB resistance using 10-day-old-seedlings in controlled environment at ICRISAT and adult plant field screening at hot-spot locations in Dhaulakuan and Ludhiana in India.  相似文献   

Identification of quantitative trait loci for yield and yield related traits in groundnut (Arachis hypogaea L.) under different water regimes in Niger and Senegal     

Issa Faye  Manish K. Pandey  Falalou Hamidou  Abhishek Rathore  Ousmane Ndoye  Vincent Vadez  Rajeev K. Varshney 《Euphytica》2015,206(3):631-647

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Citrus limonin and its glucoside inhibit colon adenocarcinoma cell proliferation through apoptosis     

Chidambara Murthy KN  Jayaprakasha GK  Kumar V  Rathore KS  Patil BS 《Journal of agricultural and food chemistry》2011,59(6):2314-2323

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Storage elevates phenolic content and antioxidant activity but suppresses antiproliferative and pro-apoptotic properties of colored-flesh potatoes against human colon cancer cell lines     

Madiwale GP  Reddivari L  Holm DG  Vanamala J 《Journal of agricultural and food chemistry》2011,59(15):8155-8166

Colored-flesh potatoes are an excellent source of health-benefiting dietary polyphenols, but are stored for up to 3-6 months before consumption. This study investigated the effect of simulated commercial storage conditions on antioxidant activity (DPPH, ABTS), phenolic content (FCR) and composition (UPLC-MS), and anticancer properties (early, HCT-116 and advanced stage, HT-29 human colon cancer cell lines) of potato bioactive compounds. Extracts from seven potato clones of differing flesh colors (white, yellow, and purple) before and after 90 days of storage were used in this study. The antioxidant activity of all clones increased with storage; however, an increase in total phenolic content was observed only in purple-fleshed clones. Advanced purple-fleshed selection CO97227-2P/PW had greater levels of total phenolics, monomeric anthocyanins, antioxidant activity and a diverse anthocyanin composition as compared with Purple Majesty. Purple-fleshed potatoes were more potent in suppressing proliferation and elevating apoptosis of colon cancer cells compared with white- and yellow-fleshed potatoes. The extracts from both fresh and stored potatoes (10-30 μg/mL) suppressed cancer cell proliferation and elevated apoptosis compared with the solvent control, but these anticancer effects were more pronounced with the fresh potatoes. Storage duration had a strong positive correlation with antioxidant activity and percentage of viable cancer cells and a negative correlation with apoptosis induction. These results suggest that although the antioxidant activity and phenolic content of potatoes were increased with storage, the antiproliferative and pro-apoptotic activities were suppressed. Thus, in the assessment of the effects of farm to fork operations on the health-benefiting properties of plant foods, it is critical to use quantitative analytical techniques in conjunction with in vitro and/or in vivo biological assays.  相似文献   

Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A,a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells     

Lijian Ding  Rinat Bar-Shalom  Dikla Aharonovich  Naoaki Kurisawa  Gaurav Patial  Shuang Li  Shan He  Xiaojun Yan  Arihiro Iwasaki  Kiyotake Suenaga  Chengcong Zhu  Haixi Luo  Fuli Tian  Fuad Fares  C. Benjamin Naman  Tal Luzzatto-Knaan 《Marine drugs》2021,19(7)

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC₅₀ = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.  相似文献   

Control of Sorghum Pests     

V. S. Rathore  R. K. Raghuwanshi  N. K. Sood  U. K. Kaushik 《国际虫害防治杂志》2013,59(2):358-360

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Wheat Pest Situation in Madhya Pradesh,India     

N. K. Sood  V. S. Rathore  R. K. Raghuwanshi 《国际虫害防治杂志》2013,59(2):361-362

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Variation in Photosynthetic Traits in Barnyard Millet (Echinocloa frumentaceae) Genotypes     

D. Subrahmanyam  V. S. Rathore 《Journal of Agronomy and Crop Science》1999,183(3):199-203

A field experiment was conducted to assess the genotypic variability in 41 divergent barnyard millet ( Echinocloa frumentaceae L.) genotypes. A high degree of variation was observed for photosynthesis and other related characteristics. However, for specific leaf weight (SLW), the differences amongst genotypes were non-significant. The seasonal means of photosynthetic rate (A), stomatal conductance (gs), transpiration (E), A/Ci ratio and total dry matter at harvest were positively and significantly correlated. None of the other parameters showed any significant relationship with total dry matter recorded at harvest. Leaf photosynthetic rate showed a positive but non-significant relationship with SLW, and it is suggested that photosynthesis rate seems to be the best selection criterion in barnyard millet. A strong and positive relationship was observed between A/E ratio, A/Ci ratio and A/gs ratio, indicating that both A/Ci ratio and A/gs had a strong influence on the water use efficiency of the leaf.  相似文献