Automated determination of alpha-tocopherol in food and feed. Part 2. Continuous flow technique     

C F Bourgeois  P R George  L A Cronenberger 《Journal of the Association of Official Analytical Chemists》1984,67(3):631-634

The proposed determination of alpha-tocopherol is a continuous flow method with fluorometric detection. The only cleanup necessary is the usual saponification. A solution of the unsaponifiable matter in isooctane is automatically assayed. Isooctane is the carrier solvent and extractions are inserted between steps. These steps are selective reactions which render the method very specific. The natural homologs of alpha-tocopherol (alpha-T) do not interfere in the determination. A procedure for blank assays allows selective inhibition of alpha-T conversions and measurement of interfering fluorescence. The method is highly sensitive, which allows the determination of alpha-T in very dilute solutions. This in turn suppresses matrix effects and renders the results reliable. The time interval between 2 peaks is 6 min, washing included, and it is possible to carry out 50 determinations per day (sample preparation not included). The system is robust and maintenance is easy. Parallel determinations of foods and feeds have been carried out with a conventional thin layer chromatographic method.  相似文献   

Soil fumigation and compost amendment alter soil microbial community composition but do not improve tree growth or yield in an apple replant site     

Shengrui Yao  George S. Abawi 《Soil biology & biochemistry》2006,38(3):587-599

Apple replant disease (ARD) is a disease complex that reduces survival, growth and yield of replanted trees, and is often encountered in establishing new orchards on old sites. Methyl bromide (MB) has been the fumigant used most widely to control ARD, but alternatives to MB and cultural methods of control are needed. In this experiment, we evaluated the response of soil microbial communities and tree growth and yield to three pre-plant soil treatments (compost amendment, soil treatment with a broad-spectrum fumigant, and untreated controls), and use of five clonal rootstock genotypes (M.7, M.26, CG.6210, G.30 and G.16), in an apple replant site in Ithaca, New York. Polymerase chain reaction (PCR)—denaturing gradient gel electrophoresis (DGGE) analysis was used to assess changes in the community composition of bacteria and fungi in the bulk soil 8, 10, 18 and 22 months after trees were replanted. PCR-DGGE was also used to compare the community composition of bacteria, fungi and pseudomonads in untreated rhizosphere soil of the five rootstock genotypes 31 months after planting. Tree caliper and extension growth were measured annually in November from 2002 to 2004. Apple yield data were recorded in 2004, the first fruiting year after planting. Trees on CG.6210 rootstocks had the most growth and highest yield, while trees on M.26 rootstocks had the least growth and lowest yield. Tree growth and yield were not affected by pre-plant soil treatment except for lateral extension growth, which was longer in trees growing in compost-treated soil in 2003 as compared to those in the fumigation treatment. Bulk soil bacterial PCR-DGGE fingerprints differed strongly among the different soil treatments 1 year after their application, with the fingerprints derived from each pre-plant soil treatment clustering separately in a hierarchical cluster analysis. However, the differences in bacterial communities between the soil treatments diminished during the second year after planting. Soil fungal communities converged more rapidly than bacterial communities, with no discernable pattern related to pre-plant soil treatments 10 months after replanting. Changes in bulk soil bacterial and fungal communities in response to soil treatments had no obvious correlation with tree performance. On the other hand, rootstock genotypes modified their rhizosphere environments which differed significantly in their bacterial, pseudomonad, fungal and oomycete communities. Cluster analysis of PCR-DGGE fingerprints of fungal and pseudomonad rhizosphere community DNA revealed two distinct clusters. For both analyses, soil sampled from the rhizosphere of the two higher yielding rootstock genotypes clustered together, while the lower yielding rootstock genotypes also clustered together. These results suggest that the fungal and pseudomonad communities that have developed in the rhizosphere of the different rootstock genotypes may be one factor influencing tree growth and yield at this apple replant site.  相似文献   

Observations of water,air and soil pollution in Israel and vicinity from the ERTS-1 imagery     

Joseph Otterman  Avihu Ginzburg  George Ohring  Yuri Mekler 《Water, air, and soil pollution》1974,3(1):53-61

Israel is a participant in the Earth Resources Technology Satellite Program (ERTS). The imagery of Israel and vicinity taken by the Multi Spectral Scanner of the ERTS-1 Satellite has been the subject of analysis in a multidisciplinary research program. The objectives of the program are mapping of agricultural crops, mainly wheat and orange groves, mapping of natural vegetation, and studies for Israeli hydrology, arid regions research, geology, and oceanography. Cases of water, air, and soil pollution have been observed in the imagery on several dates, and are reported in this paper. These are: oil slicks in the Gulf of Suez and possibly near Haifa; smoke plumes, extending over some 100 km in a nearly straight line along the Gulf of Suez, and soil pollution caused by copper mine effluents in the Arava Valley in Israel. The images of these cases of pollution are presented and ‘space signatures’, i.e., the radiometric spectral characteristics of these cases of pollution as seen from space are also given.  相似文献   

Role of air pollution in forest decline in Eastern North America     

Dale W. Johnson  George E. Taylor 《Water, air, and soil pollution》1989,48(1-2):21-43

Evidence for and against three general mechanisms by which air pollution stress may cause or contribute to forest decline in eastern North America is examined. These mechanisms are (1) soil acidification, cation nutrient depletion, and subsequent Al toxicity, (2) direct and indirect effects of gaseous pollutants on the physiology and growth of forest trees; and (3) excess nitrogen deposition and subsequent soil acidification or physiological injury. Recent studies have revealed reductions in base saturation in some sites (both polluted and pristine), but the consequences of these changes to forest health and nutrition are not resolved. Aluminum toxicity may contribute to forest decline in red spruce (Picearubens L.) in high-elevation sites, but the data are contradictory. In forested landscapes in which regional decline (reduced tree growth and/or dieback) is reported, O₃ is the only gaseous pollutant documented as a contributing factor in the eastern North America. Whereas this secondary pollutant exhibits a regional distribution and occurs at potentially phytotoxic levels, a linkage between O₃ and forest decline is substantive only for loblolly pine (Pinustaeda Sarg.) in the southeast. Studies in high elevations do not support the hypothesis that O₃ or hydrogen peroxide directly affects the growth of red spruce or fraser fir (Abiesfraseri Poir.).However, preliminary data link chronic-level O₃ exposure with indirect effects, principally changes in drought tolerance in low-elevations (e.g., P. taeda) nd winter hardiness in high elevations (e.g., P. rubens).The emerging data on the role of air pollution as an environmental stress indicate that indirect effects (i.e., responses in which pollution alters the plant's ability to compete for limited resources or withstand other environmental stresses) are more important than direct effects (e.g., foliar necrotic lesions). Nitrogen saturation has been offered as an hypothesis for P. rubens decline, but no experimental data exist supporting this hypothesis.  相似文献   

Extrusion conditions affect chemical composition and in vitro digestion of select food ingredients     

Dust JM  Gajda AM  Flickinger EA  Burkhalter TM  Merchen NR  Fahey GC 《Journal of agricultural and food chemistry》2004,52(10):2989-2996

An experiment was conducted to determine the effects of extrusion conditions on chemical composition and in vitro hydrolytic and fermentative digestion of barley grits, cornmeal, oat bran, soybean flour, soybean hulls, and wheat bran. Extrusion conditions altered crude protein, fiber, and starch concentrations of ingredients. Organic matter disappearance (OMD) increased for extruded versus unprocessed samples of barley grits, cornmeal, and soybean flour that had been hydrolytically digested. After 8 h of fermentative digestion, OMD decreased as extrusion conditions intensified for barley grits and cornmeal but increased for oat bran, soybean hulls, and wheat bran. Total short-chain fatty acid production decreased as extrusion conditions intensified for barley grits, soybean hulls, and soybean flour. These data suggest that the effects of extrusion conditions on ingredient composition and digestion are influenced by the unique chemical characteristics of individual substrates.  相似文献   

Permethrin and its two metabolite residues in seven agricultural crops     

D A George 《Journal of the Association of Official Analytical Chemists》1985,68(6):1160-1163

Metabolite residues of permethrin are not reported in the literature for most agricultural crops. This paper reports residues of permethrin and its 2 metabolites (dichlorovinyl acid and metaphenoxybenzyl alcohol) in 7 different agricultural crops (Chinese cabbage, spinach, asparagus, raspberries, green peas, turnip roots, and turnip greens). Permethrin residues declined approximately 85% within 7 days after treatment in all crops. In most cases, the acid metabolite residues peaked at 3 days, and declined after that. Translocation of residues into turnip roots was very slight; the average was less than 0.05 ppm for permethrin and alcohol metabolite residues and none was detected for the acid metabolite residue. Permethrin residues in the turnip greens averaged approximately 2 ppm for the 0.112 kg ai/ha treatment, and 6 ppm for the 0.224 kg ai/ha treatment.  相似文献   

An integrated hydro-economic modelling framework to evaluate water allocation strategies II: Scenario assessment   总被引：2，自引：0，他引：2  

Biju George  Hector MalanoBrian Davidson  Petra HellegersLuna Bharati  Sylvain Massuel 《Agricultural Water Management》2011,98(5):747-758

In this paper the results of an assessment of the hydrological and economic implications of reallocating water in the Musi sub-basin, a catchment within the Krishna Basin in India, are reported. Policy makers identified a number of different but plausible scenarios that could apply in the sub-basin, involving; supplying additional urban demand from agricultural allocations of water, implementing a number of demand management strategies, changing the timing of releases for hydropower generation, changing the crops grown under irrigation, reducing existing stream flows and allowing for more environmental flows. The framework chosen to undertake this assessment was a simulation model that measures and compares the economic values of water allocation scenarios determined from a water allocation model that accounts for supplies of groundwater and surface water across a number of regions and over a variety of uses. Policy makers are provided with the range of measures on the security of the supply of water and the social costs and benefits of reallocating water between sectors and across regions within the sub-basin. Taking water from agriculture to supply urban users has a greater impact on irrigation supplies during dry years. It was also found that changing the allocation of water between sectors, by taking it away from agriculture had a large positive economic impact on the urban sector. Yet the costs involved in undertaking such a strategy results in a significant loss in the net present value of the scheme. Stream flow reductions, if significantly large (at around 20%), were found to have a large physical and economic impact on the agricultural sector. Implementing water saving strategies in Hyderabad was found to be more cost effective than taking water from agriculture, if rainwater tanks are used to achieve this. Changing the timing of hydropower flows resulted in best meeting of irrigation demand in NSLC and NSRC. Under this scenario, the crops grown under irrigation were found to have a significant economic impact on the sub-basin, but not as large as farmers undertaking crop diversification strategies, ones which result in farmers growing less rice. The security of supplying water to different agricultural zones has significantly improved under this scenario. Finally, releasing water for environmental purposes was found to have only a minor impact on the agricultural sector.  相似文献   

Keeping an open mind?     

Anderson G 《Science (New York, N.Y.)》2005,308(5721):495

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Gametes from embryonic stem cells: a cup half empty or half full?     

Daley GQ 《Science (New York, N.Y.)》2007,316(5823):409-410

When first reported 4 years ago, gametogenesis from embryonic stem (ES) cells promised an accessible in vitro model to facilitate molecular analysis of the germ lineage. Formation of primordial germ cells is robust, but terminal gametogenesis remains inefficient and doubts about gamete function persist. Although useful for research, clinical use of ES cell-derived gametes appears a distant prospect.  相似文献   

Behavior. A marketplace in the brain?     

Ainslie G  Monterosso J 《Science (New York, N.Y.)》2004,306(5695):421-423

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