Geographical classification of honey samples by near-infrared spectroscopy: a feasibility study   总被引：3，自引：0，他引：3  

Woodcock T  Downey G  Kelly JD  O'Donnell C 《Journal of agricultural and food chemistry》2007,55(22):9128-9134

The potential of near-infrared (NIR) spectroscopy to determine the geographical origin of honey samples was evaluated. In total, 167 unfiltered honey samples (88 Irish, 54 Mexican, and 25 Spanish) and 125 filtered honey samples (25 Irish, 25 Argentinean, 50 Czech, and 25 Hungarian) were collected. Spectra were recorded in transflectance mode. Following preliminary examination by principal component analysis (PCA), modeling methods applied to the spectral data set were partial least-squares (PLS) regression and soft independent modeling of class analogy (SIMCA); various pretreatments were investigated. For unfiltered honey, best SIMCA models gave correct classification rates of 95.5, 94.4, and 96% for the Irish, Mexican, and Spanish samples, respectively; PLS2 discriminant analysis produced a 100% correct classification for each of these honey classes. In the case of filtered honey, best SIMCA models produced correct classification rates of 91.7, 100, 100, and 96% for the Argentinean, Czech, Hungarian, and Irish samples, respectively, using the standard normal variate (SNV) data pretreatment. PLS2 discriminant analysis produced 96, 100, 100, and 100% correct classifications for the Argentinean, Czech, Hungarian, and Irish honey samples, respectively, using a second-derivative data pretreatment. Overall, while both SIMCA and PLS gave encouraging results, better correct classification rates were found using PLS regression.  相似文献   

Third‐compartment cannulation in alpacas using a polyurethane gastrostomy tube     

GW Smith  MP Gerard  NB Campbell  DM Foster  SM Smith  JL Davis 《Australian veterinary journal》2009,87(12):487-491

Objective To develop a simple and effective surgical technique for third‐compartment cannulation in alpacas. Design Prospective study using six adult male alpacas. Methods General anaesthesia was induced and a polyurethane gastrostomy tube was surgically implanted into the distal portion of the third compartment. Results Three of the alpacas retained their cannulas for a 100‐day period; however, three cannulas were dislodged during the study. Two of the three dislodged cannulas were replaced during a second surgical procedure. Cannulas were well tolerated by the alpacas and all animals remained clinically healthy during the study period. Third compartment contents did not leak from the cannulation site. The tubes were manually removed following the completion of the study and the small defect in the body wall quickly healed over in all animals. Conclusion Surgical placement of polyurethane tubes designed for percutaneous endoscopic gastrostomy is a useful method of cannulating the third compartment in camelids. This technique can be used for experimental studies and possibly could be used for nutritional support and fluid therapy in sick camelids that might need long‐term care.  相似文献   

The impact of resistance on the use of second-generation anticoagulants against rats on farms in Southern England     

David Cowan  Gary Dunsford  Erica Gill  Ainsley Jones  Gerard Kerins  Alan Macnicoll  Roger Quy 《Pest management science》1995,43(1):83-93

Resistance amongst rats to second-generation anticoagulants, notably difenacoum, has been recognised since the 1970s. Although initially considered to be of practical significance, doubts were subsequently expressed that the degree of resistance was sufficient to explain ineffective rodenticide treatments. Research published elsewhere demonstrated the overriding importance of poor poisoned bait consumption, caused by the influence of certain ecological factors on rat behaviour, in reducing the effectiveness of second-generation anticoagulant treatments on farms in southern England. In this paper we consider, however, the subtle effects of resistance to anticoagulants on treatment outcome. The prevalence of resistance was increased amongst survivors of treatments and bait consumption by resistant survivors was higher than amongst susceptible survivors. The overall prevalence and degree of resistance to second-generation anticoagulants does not, however, currently represent a practical problem. Nevertheless, selection favouring increased resistance remains a long-term threat to the effectiveness of both difenacoum and bromadiolone.  相似文献   

Water balance,respiration and immobilisation in relation to deltamethrin poisoning and physical conditions in the epigeal spider Oedothorax apicatus     

Gerard A. J. M. Jagers  Op Akkerhuis  Roelof Westerhof  Nico M. Van Straalen  Jan H. Koeman 《Pest management science》1995,44(2):123-130

The effect of a pyrethroid insecticide, deltamethrin, on water loss, metabolic rate and immobilisation was studied, using the epigeal spider Oedothorax apicatus (Blackwall) (Linyphiidae) as a test species. Water loss was measured gravimetrically at different temperatures, air humidities, and doses of deltamethrin. Metabolic rate and immobilisation were measured at different temperatures and doses. Immobilisation was measured at different relative humidities and temperatures. Water loss due to evaporation increased linearly with vapour pressure deficit. The slope of the latter relationship increased with temperature, indicating an increase in cuticle permeability with temperature. In addition to water loss by evaporation, deltamethrin treatment induced water excretion. This was positively related to dose and temperature, but independent of air humidity. Metabolic rate increased with temperature and dose. Immobilisation was highest at low temperatures in combination with low air humidity. The consequences of pesticide-induced water excretion are discussed with respect to field- and laboratory-based dose-effect relationships for pyrethroids presented in the literature. The present results seem to offer an explanation for hitherto unexplained minima in temperature-response curves in pyrethroid toxicity in some arthropod species. It is concluded that simultaneous measurements of metabolic rate, water-loss rate and pesticide effects are a prerequisite for an understanding of the effects of pesticides on arthropods.  相似文献   

Biomedicine. Asthmatics breathe easier when it's SNO-ing     

Gerard C 《Science (New York, N.Y.)》2005,308(5728):1560-1561

  相似文献   

Effects of an S6 strain of Mycoplasma gallisepticum inoculation before beginning of lay on the leukocytic characteristics of commercial layers     

Peebles ED  Parker TA  Branton SL  Willeford KO  Jones MS  Gerard PD  Pharr GT  Maslin WR 《Avian diseases》2004,48(1):196-201

A clinical study was conducted on commercial layers housed in biological isolation units, within which exogenous stress factors potentially affecting bird performance were minimized. This set-up was devised in order to assess how a pre-lay inoculation of S6 strain Mycoplasma gallisepticum affects the leukocytic properties of laying chickens. Previous studies have demonstrated relative decreases in lymphocyte and relative increases in heterophil percentages in birds infected with other strains of Mycoplasma gallisepticum. However, current results showed that the differential percentages of lymphocytes were decreased, whereas those of heterophils were increased, in both sham-inoculated control birds and birds inoculated with S6 Mycoplasma gallisepticum between 19 and 26 wk of age. This study clearly shows that a pre-lay inoculation of S6 Mycoplasma gallisepticum alone had no apparent effect on the leukocyte profile of commercial layers housed in biological isolation units.  相似文献   

Physics. Sensing electrons on the edge     

Lander GH 《Science (New York, N.Y.)》2003,301(5636):1057-1059

  相似文献   

Characterization of the in vitro adhesion of Actinobacillus pleuropneumoniae to swine alveolar epithelial cells   总被引：5，自引：0，他引：5  

Van Overbeke I  Chiers K  Charlier G  Vandenberghe I  Van Beeumen J  Ducatelle R  Haesebrouck F 《Veterinary microbiology》2002,88(1):59-74

Actinobacillus pleuropneumoniae biovar 1 serotypes 2, 5a, 9 and 10 strains were tested for their ability to adhere to alveolar epithelial cells in culture. For the serotypes 5a, 9 and 10 strains, optimal adherence was observed after growth of bacterial cells in a NAD-restricted medium (0.001% NAD). This condition was also associated with the expression of a 55 kDa outer membrane protein (OMP) and of fimbriae. For the serotype 2 strain, adherence and expression of fimbriae and a 55 kDa OMP was less influenced by the growth conditions. The N-terminal amino acid sequence of the 55 kDa OMP had no homology with any known sequence, suggesting that it is an as yet unknown protein. Adherence capabilities were significantly reduced following treatment of the bacteria with proteolytic enzymes or heat. These findings suggest that proteins are involved in adhesion. The hydrophobic bond-breaking agent tetramethylurea was unable to inhibit the adherence of A. pleuropneumoniae to alveolar epithelial cells. Treatment of the bacteria with sodium metaperiodate resulted in lower adhesion scores for the serotypes 2 and 9 strains but the inhibition of adhesion was clearly lower than after treatment with proteolytic enzymes. This indicates that, besides proteins, carbohydrates might also be involved in adhesion of A. pleuropneumoniae to alveolar epithelial cells. The finding that inhibition of adhesion was very high when bacteria were treated with a combination of sodium metaperiodate and pronase also suggests that more than one adhesin is involved.  相似文献   

Relation between Habitual Diet and Canine Mammary Tumors in a Case-Control Study   总被引：2，自引：0，他引：2  

Dolores Pérez Alenza  Gerard R. Rutteman  Laura Peña  Anton C. Beynen  Pedro Cuesta 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):132-139

In the present case-control study several dietary and nutritional factors were investigated to determine if a relationship exists between diet and development of mammary tumors in female dogs. Control female dogs (n = 86) were compared with a case group of dogs (n = 102) with dysplasias or tumors of the mammary gland. A questionnaire providing information on the dog's body conformation and dietary and reproductive histories was answered by the owners. Serum selenium and retinol concentrations and the fatty acid profile in subcutaneous adipose tissue were analyzed as indicators of nutritional status. Obesity at 1 year of age and 1 year before the diagnosis of mammary nodules was found to be significantly related to a higher prevalence of mammary tumors and dysplasias. The intake of homemade meals (compared to that of commercial foods) was also significantly related to a higher incidence of tumors and dysplasias. Other significant risk factors were a high intake of red meat, especially beef and pork, and a low intake of chicken. The subcutaneous fatty acid profile and the serum selenium concentration were not significantly different in the cases and the controls, with the exception of C18:1 fatty acid (oleic acid) content, which was significantly higher in the cases than in healthy controls. Serum retinol concentration was significantly lower in the cases than in the controls. In the multivariate analysis, older age, obesity at 1 year of age, and a high red meat intake were independently and significantly associated with the risk of developing mammary tumor and dysplasias.  相似文献   

Controlling pests of tropical legumes; current recommendations and future strategies     

Brian M. Gerard 《Pest management science》1978,9(5):465-477

Legumes have evolved effective defences against many pests by accumulating high levels of poisonous or indigestible compounds, pubescence and vigorous growth. These defences are greatly reduced in many cultivars of cowpea and common bean, which are susceptible to a succession of pest complexes. Stem pests and defoliators may kill seedlings but well-established plants tolerate heavy insect damage to leaves, flowers and green pods before pod or grain yields are reduced; virus vectors and root-knot nematode may be of greater importance. Generally, pod-borers, pod-sucking bugs and seed beetles are the main key pests. Control programmes are based on post-flowering treatments with broad spectrum insecticides. Battery-powered, controlled-droplet application machines are ousting knapsack sprayers in small-scale farming and competing with tractors and aircraft in larger units; subsistence cropping is still predominant and most legume crops remain unsprayed. Consequently, current emphasis is placed on developing cultivars with high yield potential and multiple pest and disease resistance. Insect infestations are lower in most other major legume crops largely due to pubescence (e.g. soybean, blackgram, greengram), fruiting in the dry season (e.g. pigeonpea, chickpea) or geocarpy (e.g. groundnut). Future entomological research will presumably concentrate on assessing the status of pests on resistant cultivars and introducing integrated control methods for the key pests.  相似文献