Heat shock proteins expression in canine intracutaneous cornifying epithelioma and squamous cell carcinoma   总被引：1，自引：0，他引：1  

Romanucci M  Bongiovanni L  Marruchella G  Marà M  di Guardo G  Preziosi R  della Salda L 《Veterinary dermatology》2005,16(2):108-116

Heat shock proteins (HSPs) are strongly implicated in the control of cell growth, differentiation and biological behaviour of many human cutaneous neoplasms. To our knowledge, no data have been published in the veterinary literature concerning either normal or neoplastic skin. In this study, the immunohistochemical expression of Hsp27, Hsp72 and Hsp73 was evaluated in normal canine skin, 14 intracutaneous cornifying epitheliomas (ICE), 10 well-differentiated and 5 moderately differentiated squamous cell carcinomas (SCC). Expression was correlated with the histological degree of keratinocyte differentiation and proliferation, and investigated as to its usefulness in the differential diagnosis of these canine tumours. In normal epidermis, Hsp27 exhibited cytoplasmic labelling in the spinous and granular layers, whereas in neoplastic tissues it was detected particularly in those areas showing squamous differentiation. Hsp72 immunoreactivity was more intense in ICE and well-differentiated SCC than in normal skin; however, reduced immunolabelling was observed in moderately differentiated SCC. Unlike Hsp72, Hsp73 showed less intense labelling in ICE and well-differentiated SCC than in normal epithelium and an increased positivity in moderately differentiated SCC. These results indicate that HSP immunoreactivity differs between normal and neoplastic canine skin. Hsp27 expression seems to correlate directly with cellular differentiation; by contrast, the involvement of Hsp72/73 in proliferation and differentiation of tumour cells remains controversial. The pattern and intensity of immunolabelling of each investigated HSP did not show, however, significant differences between ICE and SCC; therefore, they do not seem to be useful in the differential diagnosis of these two canine tumours.  相似文献   

Herbicide risk assessment for non-target aquatic plants: sulfosulfuron--a case study     

Davies J  Honegger JL  Tencalla FG  Meregalli G  Brain P  Newman JR  Pitchford HF 《Pest management science》2003,59(2):231-237

Herbicides entering the aquatic environment by spray drift, run-off and leaching to field drains may cause adverse effects on non-target aquatic vegetation. The potential for such effects has typically been evaluated from tests with floating, monocotyledonous Lemna sp. However, concern has been expressed as to whether this species could be used to indicate potential effects on other vegetation types, particularly rooted, submerged, emergent or dicotyledonous species. In 1997, the Centre for Aquatic Plant Management undertook development of new tests based on the additional species, Glyceria maxima (Hartm) Holmb, Lagarosiphon major (Ridl) Moss and Myriophyllum spicatum L. The resulting methodology was used to assess the effects of the sulfonylurea herbicide, sulfosulfuron on these species. Data presented here demonstrate that exposure to initial sulfosulfuron concentrations of 3.33 microg litre(-1) for up to 21 days was tolerated by these species and that adverse effects were observed only when plants were exposed to initial concentrations of 3.33 and 10 microg litre(-1) for 70 days. As the occurrence of such high initial concentrations for long periods is unlikely in the aquatic environment, sulfosulfuron is not expected to have adverse effects on the growth of these species. This study has also demonstrated that G maxima, L major and M spicatum grown in small outdoor tanks can be used successfully to assess the effects of crop-protection products on non-target aquatic flora.  相似文献   

Somatic Cell Nuclear Transfer in Horses     

Cesare Galli  Irina Lagutina  Roberto Duchi  Silvia Colleoni  Giovanna Lazzari 《Reproduction in domestic animals》2008,43(S2):331-337

The cloning of equids was achieved in 2003, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay was because of the limited development in the horse of more classical-assisted reproductive techniques required for successful cloning, such as oocyte maturation and in vitro embryo production. When these technologies were developed, the application of cloning also became possible and cloned horse offspring were obtained. This review summarizes the main technical procedures that are required for cloning equids and the present status of this technique. The first step is competent oocyte maturation, this is followed by oocyte enucleation and reconstruction, using either zona-enclosed or zona-free oocytes, by efficient activation to allow high cleavage rates and finally by a suitable in vitro embryo culture technique. Cloning of the first equid, a mule, was achieved using an in vivo -matured oocytes and immediate transfer of the reconstructed embryo, i.e. at the one cell stage, to the recipient oviduct. In contrast, the first horse offspring was obtained using a complete in vitro procedure from oocyte maturation to embryo culture to the blastocyst stage, followed by non-surgical transfer. Later studies on equine cloning report high efficiency relative to that for other species. Cloned equid offspring reported to date appear to be normal and those that have reached puberty have been confirmed to be fertile. In summary, horse cloning is now a reproducible technique that offers the opportunity to preserve valuable genetics and notably to generate copies of castrated champions and therefore, offspring from those champions that would be impossible to obtain otherwise.  相似文献   

Adenoma of the Uterine Tube in the Bitch: Two Case Reports     

Marino  G.  Quartuccio  M.  Cristarella  S.  Nic&#;tina  P. A.  Zangh&#;  A. 《Veterinary research communications》2007,31(1):173-175

Veterinary Research Communications - Marino, G., Quartuccio, M., Cristarella, S., Nicòtina, P.A. and Zanghì, A., 2007. Adenoma of the uterine tube in the bitch: two case reports....  相似文献   

Effect of antagonistic<Emphasis Type="Italic">Fusarium</Emphasis> spp. and of different commercial biofungicide formulations on Fusarium wilt of lettuce     

Giovanna Gilardi  Angelo Garibaldi  Maria Lodovica Gullino 《Phytoparasitica》2007,35(5):457-465

Five experimental trials were carried out to test different biological control agents against Fusarium wilt of lettuce, cause byFusarium oxysporum f.sp.lactucae. In the presence of a very high disease incidence, the best results in terms of disease control as well as increased growth response were shown byTrichoderma harzianum T 22 (RootShield), which, at 3 gl ⁻¹ of substrate, provided very consistent results.F. oxysporum IF 23 gave good disease control but in two out of five trials reduced the biomass produced. Less consistent, but still significant results were provided byF oxysporium MSA 25, at 3 gl ⁻¹ of substrate, and byTrichoderma viride TV 1. The twoF. oxysporum agents Fo 251/2 and Fo 47 and the mixture ofT. harzianum + T. viride (Remedier) partially reduced disease incidence but were less effective than the above mentioned. Less interesting results were offered byStreptomyces griseoviridis (Mycostop). The results obtained show that biological control can play a role in the management of Fusarium wilt of lettuce.  相似文献   

Bilateral Testicular Leydig Cells Tumour in a Donkey     

V Galofaro  G Rapisarda  G Ferrara  F Marino 《Reproduction in domestic animals》2007,42(1):109-110

A rare case of a bilateral Leydig cells tumour located in the testis of a 15-year-old donkey slaughtered normally is presented. Histologically, the testicular tumour showed neoplastic Leydig cells arranged in solid growth as a pseudoadenomatous packet type. To our knowledge, this is the first bilateral Leydig cells tumour, a neoplasm never reported in the donkey.  相似文献   

Harmonised pesticide risk trend indicator for food (HAPERITIF): The methodological approach     

Calliera M  Finizio A  Azimonti G  Benfenati E  Trevisan M 《Pest management science》2006,62(12):1168-1176

To provide a harmonised European approach for pesticide risk indicators, the Sixth EU Framework Programme recently financed the HAIR (HArmonised environmental Indicators for pesticide Risk) project. This paper illustrates the methodology underlying a new indicator-HAPERITIF (HArmonised PEsticide RIsk Trend Indicator for Food), developed in HAIR, for tracking acute and chronic pesticide risk trends for consumers. The acute indicator, HAPERITIF(ac), is based on the ratio between an estimated short-term intake (ESTI), calculated as recommended by the World Health Organisation (WHO), and the acute reference dose (ARfD); the chronic indicator HAPERITIF(chr) is based on the ratio between an estimated daily intake (EDI) and the admissible daily intake (ADI). HAPERITIF can be applied at different levels of aggregation. Each level gives information for proper risk management of pesticides to reduce the risk associated with food consumption. An example of application using realistic scenarios of pesticide treatments on a potato crop in central-northern Italy is reported to illustrate the different steps of HAPERITIF.  相似文献   

A dose-finding,long-term study on the use of calcium chloride in saline solution as a method of nonsurgical sterilization in dogs: evaluation of the most effective concentration with the lowest risk     

Raffaella Leoci  Giulio Aiudi  Fabio Silvestre  Elaine A Lissner  Fabio Marino  Giovanni M Lacalandra 《Acta veterinaria Scandinavica》2014,56(1)

Background

Canine overpopulation is a global issue with serious health and welfare implications. Nonsurgical methods of sterilization could yield positive impacts on this problem, but no long-term data on such methods are available. The objective of the current investigation was to determine the effects of intratesticular injections of calcium chloride dihydrate (CaCl₂) in saline in dogs over a one year period. Five concentrations (0%, 10%, 20%, 30%, 60%) of CaCl₂ in saline were administered via intratesticular injection to groups of 10 dogs each. Total sperm count and motility, blood levels of testosterone, and side effects were examined at 0, 2, 6, and 12 months post-injection (PI). Testicular size and semen volume were examined at 0 and 12 months PI.

Results

Total sperm count, semen volume and testosterone showed significant dose-dependent decreases upon treatment with 10%-60% CaCl₂ compared with either the control group (0% CaCl₂) or baseline for each treatment group. Azoospermia was achieved for at least 12 months PI in 60% and 80% of treated dogs after administration of a 10% and 20% CaCl₂, respectively. Treatment with 30% or 60% CaCl₂ resulted in azoospermia in 100% of dogs, but more side effects were observed, while no side effects were noticed at lower doses. For each treatment group, testosterone levels had decreased an average of 35%-70% at 6 months following treatment. However, testosterone levels rebounded by the 12-month time point in all groups except the highest dosage group (60% CaCl₂), which remained at the low end of physiological range throughout the study. Sperm motility dropped to zero or near zero in all dogs treated with CaCl₂. Testicular size was significantly smaller at 12 months PI for all groups when compared to baseline.

Conclusions

This first long-term study confirms reports of the efficacy of CaCl₂ sterilization. However, at dosages free of adverse events, calcium chloride in saline may not provide permanent sterilization as previously believed. Future work should explore optimized solvents to increase the permanence of the well-tolerated 20% formulation.  相似文献   

Isotopic evidence for neogene hominid paleoenvironments in the kenya rift valley     

Kingston JD  Hill A  Marino BD 《Science (New York, N.Y.)》1994,264(5161):955-959

Bipedality, the definitive characteristic of the earliest hominids, has been regarded as an adaptive response to a transition from forested to more-open habitats in East Africa sometime between 12 million and 5 million years ago. Analyses of the stable carbon isotopic composition (delta(13)C) of paleosol carbonate and organic matter from the Tugen Hills succession in Kenya indicate that a heterogeneous environment with a mix of C3 and C4 plants has persisted for the last 15.5 million years. Open grasslands at no time dominated this portion of the rift valley. The observed delta(13)C values offer no evidence for a shift from more-closed C3 environments to C4 grassland habitats. If hominids evolved in East Africa during the Late Miocene, they did so in an ecologically diverse setting.  相似文献   

Effects of different irrigation regimes on a super-high-density olive grove cv. “Arbequina”: vegetative growth,productivity and polyphenol content of the oil     

F. P. Marra  G. Marino  A. Marchese  T. Caruso 《Irrigation Science》2016,34(4):313-325

The effects of multiple irrigation regimes on the relationships among tree water status, vegetative growth and productivity within a super-high-density (SHD) “Arbequina” olive grove (1950 tree/ha) were studied for three seasons (2008–2010). Five different irrigation levels calculated as percentage of crop irrigation requirement using FAO procedures (Allen et al. in Crop evapotranspiration. Guidelines for computing crop water requirements. Irrigation and drainage paper 56. FAO, Rome, 1998) were imposed during the growing season. Periodically during the growing season, daytime stem water potential (Ψ _STEM), inflorescences per branch, fruits per inflorescence and shoot absolute growth rate were measured. Crop yield, fruit average fresh weight and oil polyphenol content were measured after harvest. The midday Ψ _STEM ranged from ?7 to ?1.5 MPa and correlated well enough with yield efficiency, crop density and fruit fresh weight to demonstrate its utility as a precise method for determining water status in SHD olive orchards. The relationships between midday Ψ _STEM and the horticultural parameters suggest maintaining Ψ _STEM values between ?3.5 and ?2.5 MPa is optimal for moderate annual yields of good quality oil. Values below ?3.5 MPa reduced current season productivity, while values over ?2.5 MPa were less effective in increasing productivity, reduced oil quality and produced excessive crop set that strongly affected vegetative growth and fruit production the following season. On the basis of the result given here, irrigation scheduling in the new SHD orchards should be planned on a 2-year basis and corrected annually based on crop load. Collectively, these results suggest that deficit irrigation management is a viable strategy for SHD olive orchards.  相似文献