Synthesis,insecticidal activity,and ensembled docking of nitroguanidines bearing S- and R-proline     

Alfredo Rodríguez-Hernández  Israel Bonilla-Landa  Abraham Vidal-Limon  Arturo Ibarra-Juárez  Felipe Barrera-Méndez  Francisco Javier Enríquez Medrano  Ramón E. Díaz de León  José Luis Olivares-Romero 《Pest management science》2023,79(5):1912-1921

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Natural hybridization between wheat (Triticum aestivum L.) and its wild relatives Aegilops geniculata Roth and Aegilops triuncialis L.     

Iñigo Loureiro  María Concepción Escorial  María Cristina Chueca 《Pest management science》2023,79(6):2247-2254

BACKGROUND

Cultivated bread wheat (Triticum aestivum L.) spontaneously hybridizes with wild/weedy related Aegilops populations, but little is known about the actual rates at which this hybridization occurs under field conditions. It is very important to provide reliable empirical data on this phenomenon in order to assess the potential crop–wild introgression, especially in the context of conducting risk assessments for the commercialization of genetically modified (GM) wheat, as gene flow from wheat to Aegilops species could transfer into the wild species genes coding for traits such as resistance to herbicides, insects, diseases or environmental stresses.

RESULTS

The spontaneous hybridization rates between wheat and A. geniculata and A. triuncialis, which are very abundant in the Mediterranean area, have been estimated for the first time in the northern part of the Meseta Central, the great central plateau which includes the largest area of wheat cultivation in Spain. Hybridization rates averaged 0.12% and 0.008% for A. geniculata and A. triuncialis, respectively. Hybrids were found in 26% of A. geniculata and 5% of A. triuncialis populations, at rates that can be ≤3.6% for A. geniculata and 0.24% for A. triuncialis.

CONCLUSION

The detection of Aegilops spp.–wheat hybrids in Aegilops populations indicates that gene flow can occur, although wheat is considered a crop with a low-to-medium risk for transgene escape. These data on field hybridization rates are essential for GM wheat risk assessment purposes. © 2023 Society of Chemical Industry.  相似文献   

Genetic basis and origin of resistance to acetolactate synthase inhibitors in Amaranthus palmeri from Spain and Italy     

Alfredo Manicardi  Laura Scarabel  Josep María Llenes  José María Montull  María Dolores Osuna  Joel Torra Farré  Andrea Milani 《Pest management science》2023,79(12):4886-4896

BACKGROUND

Amaranthus palmeri is an aggressive annual weed native to the United States, which has become invasive in some European countries. Populations resistant to acetolactate synthase (ALS) inhibitors have been recorded in Spain and Italy, but the evolutionary origin of the resistance traits remains unknown. Bioassays were conducted to identify cross-resistance to ALS inhibitors and a haplotype-based genetic approach was used to elucidate the origin and distribution of resistance in both countries.

RESULTS

Amaranthus palmeri populations were resistant to thifensulfuron-methyl and imazamox, and the 574-Leu mutant ALS allele was found to be the main cause of resistance among them. In two Spanish populations, 376-Glu and 197-Thr mutant ALS alleles were also found. The haplotype analyses revealed the presence of two and four distinct 574-Leu mutant haplotypes in the Italian and Spanish populations, respectively. None was common to both countries, but some mutant haplotypes were shared between geographically close populations or between populations more than 100 km apart. Wide genetic diversity was found in two very close Spanish populations.

CONCLUSION

ALS-resistant A. palmeri populations were introduced to Italy and Spain from outside Europe. Populations from both countries have different evolutionary histories and originate from independent introduction events. ALS resistance then spread over short and long distances by seed dispersal. The higher number and genetic diversity among mutant haplotypes from the Spanish populations indicated recurrent invasions. The implementation of control tactics to limit seed dispersal and the establishment of A. palmeri is recommended in both countries. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen     

Miguel Mellado  Lucía M. Treviño  María I. Chavez  Francisco G. Véliz  Ulises Macías-Cruz  Leonel Avendaño-Reyes  José E. García 《Reproduction in domestic animals》2023,58(1):39-47

The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.  相似文献   

Validation of new automated turbidimetric immunoassays for the measurement of haptoglobin and inter-α-trypsin inhibitor heavy chain H4 specific for the bovine species     

Anna Bassols  José Angel Robles-Guirado  Laura Arroyo  Lourdes Soler  Natalia García  Raquel Pato  Raquel Peña  Yolanda Saco  Ramon Armengol  Fermín Lampreave  María A. Alava  Francesca Canalias  Matilde Piñeiro 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(Z1):64-74

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Using midday stem water potential for scheduling deficit irrigation in mid–late maturing peach trees under Mediterranean conditions     

José Manuel Mirás-Avalos  Francisco Pérez-Sarmiento  Rosalía Alcobendas  Juan José Alarcón  Oussama Mounzer  Emilio Nicolás 《Irrigation Science》2016,34(2):161-173

Irrigation techniques that reduce water applications are increasingly applied in areas with scarce water resources. In this study, the effect of two regulated deficit irrigation (RDI) strategies on peach [Prunus persica (L.) Batsch cv. “Catherine”] performance was studied over three growing seasons. The experimental site was located in Murcia (SE Spain), a Mediterranean region. Two RDI strategies (restricting water applications at stage II of fruit development and postharvest) based on stem water potential (Ψ_s) thresholds (?1.5 and ?1.8 MPa during fruit growth and ?1.5 and ?2.0 MPa during postharvest) were compared to a fully irrigated control. Soil water content (θ_v), Ψ_s, gas exchange parameters, vegetative growth, crop load, yield and fruit quality were determined. RDI treatments showed significantly lower values of θ_v and Ψ_s than control trees when irrigation water was restricted, causing reductions in stomatal conductance and photosynthesis rates. Vegetative growth was reduced by RDI, as lower shoot lengths and pruning weights were observed under those treatments when compared to control. However, fruit size and yield were unaffected, and fruit quality was slightly improved by RDI. Water savings from 43 to 65 % were achieved depending on the year and the RDI strategy, and no negative carryover effect was detected during the study period. In conclusion, RDI strategies using Ψ_s thresholds for scheduling irrigation in mid–late maturing peach trees under Mediterranean conditions are viable options to save water without compromising yield and even improving fruit quality.  相似文献   

Irrigation scheduling of plastic greenhouse vegetable crops based on historical weather data     

Santiago Bonachela  Alicia María González  María Dolores Fernández 《Irrigation Science》2006,25(1):53-62

Irrigation scheduling based on the daily historical crop evapotranspiration (ET_h) data was theoretically and experimentally assessed for the major soil-grown greenhouse horticultural crops on the Almería coast in order to improve irrigation efficiency. Overall, the simulated seasonal ET_h values for different crop cycles from 41 greenhouses were not significantly different from the corresponding values of real-time crop evapotranspiration (ET_c). Additionally, for the main greenhouse crops on the Almería coast, the simulated values of the maximum cumulative soil water deficit in each of the 15 consecutive growth cycles (1988–2002) were determined using simple soil-water balances comparing daily ET_h and ET_c values to schedule irrigation. In most cases, no soil-water deficits affecting greenhouse crop productivity were detected, but the few cases found led us to also assess experimentally the use of ET_h for irrigation scheduling of greenhouse horticultural crops. The response of five greenhouse crops to water applications scheduled with daily estimates of ET_h and ET_c was evaluated in a typical enarenado soil. In tomato, fruit yield did not differ statistically between irrigation treatments, but the spring green bean irrigated using the ET_h data presented lower yield than that irrigated using the ET_c data. In the remaining experiments, the irrigation-management method based on ET_h data was modified to consider the standard deviation of the inter-annual greenhouse reference ET. No differences between irrigation treatments were found for productivity of pepper, zucchini and melon crops.  相似文献   

Modeling soil water dynamics in a drip-irrigated intercropping field under plastic mulch     

Xianyue Li  Haibin Shi  Jiří Šimůnek  Xuewen Gong  Zunyuan Peng 《Irrigation Science》2015,33(4):289-302

Intercropping, drip irrigation, and the use of plastic mulch are important management practices, which can, when utilized simultaneously, increase crop production and save irrigation water. Investigating soil water dynamics in the root zone of the intercropping field under such conditions is essential in order to understand the combined effects of these practices and to promote their wider use. However, not much work has been done to investigate soil water dynamics in the root zone of drip-irrigated, strip intercropping fields under plastic mulch. Three field experiments with different irrigation treatments (high T1, moderate T2, and low T3) were conducted to evaluate soil water contents (SWC) at different locations, for different irrigation treatments, and with respect to dripper lines and plants (corn and tomatoes). Experimental data were then used to calibrate the HYDRUS (2D/3D) model. Comparison between experimental data and model simulations showed that HYDRUS (2D/3D) described different irrigation events and SWC in the root zone well, with average relative errors of 10.8, 9.5, and 11.6 % for irrigation treatments T1, T2, and T3, respectively, and with corresponding root mean square errors of 0.043, 0.035, and 0.040 cm³ cm^?3, respectively. The results showed that the SWC in the shallow root zone (0–40 cm) was lower under non-mulched locations than under mulched locations, irrespective of the irrigation treatment, while no significant differences in the SWC were observed in the deeper root zone (40–100 cm). The SWC in the shallow root zone was significantly higher for the high irrigation treatment (T1) than for the low irrigation treatment, while, again, no differences were observed in the deeper root zone. Simulations of two-dimensional SWC distributions revealed that the low irrigation treatment (T3) produced serious severe water stress (with SWCs near the wilting point) in the 30–40 cm part of the root zone, and that using separate drip emitter lines for each crop is well suited for producing the optimal soil water distribution pattern in the root zone of the intercropping field. The results of this study can be very useful in designing an optimal irrigation plan for intercropped fields.  相似文献   

Monitoring forage production for farmers’ decision making     

Gonzalo Grigera  Martín OesterheldFernando Pacín 《Agricultural Systems》2007

Objective management of grazing livestock production systems needs monitoring of forage production at the managerial unit level. Our objectives were to develop a system that routinely estimates forage above-ground net primary production (ANPP) at the spatial and temporal resolution required by farmers in the Pampas of Argentina, and to facilitate adoption of the system by end users as a managerial support tool. Our approach was based on the radiation use efficiency (RUE) logic, which proposes that ANPP is determined by the amount of photosynthetically active radiation absorbed by the canopy (APAR), and the efficiency with which that energy is transformed in above-ground dry matter (radiation use efficiency, RUE). APAR is the product of incoming photosynthetically active radiation (PAR) and the fraction absorbed by the canopy (fPAR). We estimated fPAR as a non-linear function of MODIS normalized difference vegetation index (NDVI). RUE was empirically estimated for the two principal forage resources of the region, yielding the following relations: ANPP = 0.6 × APAR + 12, (R² = 0.86; p < 0.001; n = 18) for the upland sown pastures, and ANPP = 0.27 × APAR + 26, (R² = 0.74; p < 0.001; n = 18) for the lowland naturalized pastures, with ANPP in g/m²/60 days and APAR in MJ/m²/60 days. The models were able to predict independent ANPP values with acceptable accuracy. Computational procedures were automated and run in a Relational Data Base Manager System that stored and managed all the information. The system is currently monitoring 212,794 ha in 83 farms and provides monthly ANPP values for the previous month and a history of the last 6 years. The data so generated show ANPP differences between the two major forage resources, considerable variability of a given month’s ANPP among years and paddocks, and contrasting among-farm differences in the efficiency of conversion of ANPP and forage supplements into beef production. The system was well accepted by end users who utilize it mainly for making near real time decisions according to last month ANPP, and explaining results of previous production cycles by incorporating ANPP as an explicative variable. However, there were differences among farmers in the degree of utilization, apparently related to the advisor’s attitude toward this new technology. Our results indicate that (1) forage production of large extensions can be monthly monitored at the paddock level by a small laboratory with capabilities in geographic information systems, and (2) advisors and farmers apply this information to their managerial decisions.  相似文献   

Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum     

Zuzana Jánošíková  Emília Ondrušková  Katarína Adamčíková 《Forest Pathology》2023,53(5):e12826

Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species.  相似文献