Comprehensive transcriptional profiling and functional study of lymphangiogenic growth factor in placentome of early-stage pregnant water buffalo     

Pooja A. R.  Yogesh Pandey  Meeti Punetha  Anju Kala  Hari Abdul Samad  Gyanendra Singh  Vikrant Singh Chouhan 《Reproduction in domestic animals》2023,58(1):60-72

The aim of the present study was to evaluate the expression and localization of lymphangiogenic factors (VEGF-C and VEGF-D), their receptor (VEGFR3) and lymphatic endothelial marker (LYVE1) in buffalo placenta during early pregnancy [EP], and to investigate the functional role of lymphangiogenic growth factors in placental lymphangiogenesis. The mRNA and protein expression of VEGF-C, VEGF-D, their receptor VEGFR3 and LYVE1 showed significant expression in EP1 (29–42 days) and EP2 stages (51–82 days) both in caruncle (maternal part) and cotyledon (foetal part) of the buffalo placenta. Immunoreactivity of VEGF-C, VEGF-D and LYVE1 was observed around the endometrial gland, in lymphatics and trophoblast cells, whereas VEGFR3 mainly localized in lymphatics of the caruncle and cotyledons. Cultured trophoblast cells were treated with VEGF-C/VEGF-D (50, 100 and 150 ng/ml) and combined doses of VEGF-C and VEGF-D (150 ng/ml) each for different time durations (24, 48 and 72 h). The mRNA expression of LYVE1 and PCNA was significantly (p < .001) upregulated with VEGF-C and VEGF-D and combined treatment (@150 ng/ml), as well as significantly downregulating Caspase-3 at 48 and 72 h. Thus, the present study provides evidence that lymphangiogenic factors are expressed in buffalo placental compartments and they may play a significant role in the regulation of placental function in water buffaloes.  相似文献   

Effect of irrigation on tillering in wheat,triticale and barley in a water-limited environment     

G. S. Chaturvedi  P. K. Aggarwal  A. K. Singh  M. G. Joshi  S. K. Sinha 《Irrigation Science》1981,2(4):225-235

Summary The effect of irrigation on tillering and tiller mortality in varieties of wheat (Triticum aestivum and T. durum), triticale and barley was studied under field conditions. Low temperature in the early stages of growth promoted production of tillers whereas increase in temperature during extension growth phase increased tiller mortality. More than 1000 tillers m^–2 were produced with five irrigations but 40% or more died. With limited water availability tiller production was reduced but so was their mortality. Grain yield in wheat and triticale was positively correlated with productive tillers and negatively correlated with the maximum number of tillers produced in wheat and barley grown under limited irrigation conditions. Varieties with a capacity to produce fewer tillers were identified. Some of them proved more stable in yield. No correlation was found between tiller number and grain yield in the frequently irrigated treatment.  相似文献   

Kinematic-wave model for soil-moisture movement with plant-root extraction     

V. P. Singh  E. S. Joseph 《Irrigation Science》1994,14(4):189-198

A kinematic-wave model is developed for simulating the movement of soil moisture in unsaturated soils with plants. The model involves three free boundaries. Analytical solutions are derived when the plant roots are assumed to extract moisture at a constant rate and the upstream boundary condition is independent of time. Numerical solutions are the only resort when the moisture extraction and the upstream boundary condition both depend on time.  相似文献   

Errors of kinematic-wave and diffusion-wave approximations for time-independent flows in infiltrating channels     

V. P. Singh  V. Aravamuthan  E. S. Joseph 《Irrigation Science》1994,15(2-3):137-146

Time-independent (or steady-state) cases of channel flow were treated and errors of the kinematic-wave and diffusion-wave approximations derived for finite flow at the upstream end. The diffusion-wave approximation was found to be in excellent agreement with the dynamic wave representation, with error magnitudes of 0.2% for values of KF ₀ ² 7.5, where K is the kinematic-wave number and f ₀ is the Froude number. Even for small values of KF ₀ ² (e.g., KF ₀ ² =0.75), the errors were typically in the range of 1.3 to 3.7%. The approximate analytical diffusion-wave solution performed poorly with error magnitudes greater than 30% even for large values of KF ₀ ² . The kinematic-wave approximation was also found to be in good agreement with the dynamic-wave representation with errors of about 1.2% for KF ₀ ² =7.5 and varying from 15 to 44% for KF ₀ ² =0.75.  相似文献   

A mathematical model for border irrigation II. Vertical and horizontal recession phases     

V. P. Singh  F. X. Yu 《Irrigation Science》1987,8(3):175-190

Summary This paper, second in a series of three, develops a mathematical model, using the volume balance approach, to simulate vertical and horizontal recession of border irrigation. An equation is proposed for computing Manning's roughness factor N in both laminar and transitional flow regimes in recession phases. The model has four parameters which can be determined experimentally. Experimental data from ten vegetated as well as nonvegetated borders were used to verify the model. Average difference (AD) between calculated and observed vertical recession times was less than 4.4 min, and between calculated and observed horizontal recession times less than 4.6 min for the ten experimental data sets. Average relative error (ARE) in computed horizontal recession was less than 13% for these data sets. The model was found to be especially accurate for Reynold's number between 1,800 and 2,500.  相似文献   

Effect of irrigation on tillering in wheat,triticale and barley in a water-limited environment     

G. S. Chaturvedi  P. K. Aggarwal  A. K. Singh  M. G. Josh  S. K. Sinha 《Irrigation Science》1982,3(3):209-209

  相似文献   

Surface drainage requirement of crops: Application of a piecewise linear model for evaluating submergence tolerance     

S. K. Gupta  R. K. Singh  R. S. Pandey 《Irrigation and Drainage Systems》1992,6(3):249-261

Crop tolerance to land submergence is an important criterion for designing a surface drainage system for agricultural lands. This paper collates the available data from various places in India related to the studies on the submergence tolerance of crops. The paper hypothesizes that a piecewise linear model could be used to describe crop response to land submergence. According to this hypothesis, there would be no yield decline for a few initial days of submergence. If submergence continues beyond this period then there would be linear decline in yield. The unknown parameters in the model are: optimum yield, threshold time and the slope which represents the per cent yield reduction per day of additional submergence beyond the threshold.Data in respect of wheat, pigeon peas, cowpeas, pearlmillet, maize and groundnuts indicate that the model describes the data well, although in many cases the threshold is 0.0. The yield reduction varies from 5.3 to 23.2% for each day of submergence beyond the threshold. It appears that to allow for more than 1–2 days of submergence will result in more than 10% reducation in yield of dryfoot crops. For the maize crop, the seedling stage is the most sensitive stage followed by the silking stage. The grain formation stage is the least sensitive, although even at this stage the threshold is 0.0 and yield reduction is 9.3% for each day of submergence beyond the threshold. The data for 9 test crops from Texas and Venezuela were well described by the model. It is concluded that the piecewise linear model is a useful tool for describing submergence tolerance of crops and for working out surface drainage requirements for a given level of yield reduction. Frequency analysis of the daily rainfall data from some selected locations indicates that there is every likelihood of submergence at most of the stations. It is suggested that there is an urgent need for developing wet farming techniques analogous to dry farming techniques.  相似文献   

An improved Lewis-Milne equation for the advance phase of border irrigation     

V. P. Singh  P. D. Scarlatos  S. N. Prasad 《Irrigation Science》1990,11(1):1-6

Summary The Lewis-Milne (LM) equation has been widely applied for design of border irrigation systems. This equation is based on the concept of mass conservation while the momentum balance is replaced by the assumption of a constant surface water depth. Although this constant water depth depends on the inflow rate, slope and roughness of the infiltrating surface, no explicit relation has been derived for its estimation. Assuming negligible border slope, the present study theoretically treats the constant depth in the LM equation by utilizing the simple dam-break wave solution along with boundary layer theory. The wave front is analyzed separately from the rest of the advancing water by considering both friction and infiltration effects on the momentum balance. The resulting equations in their general form are too complicated for closed-form solutions. Solutions are therefore given for specialized cases and the mean depth of flow is presented as a function of the initial water depth at the inlet, the surface roughness and the rate of infiltration. The solution is calibrated and tested using experimental data.Abbreviations a (t) advance length - c mean depth in LM equation - c _f friction factor - c _h Chezy's friction coefficient - g acceleration due to gravity - h(x, t) water depth - h ₀ water depth at the upstream end - i() rate of infiltration - f(x, t) discharge - q₀ constant inflow discharge - S _f energy loss gradient or frictional slope - S₀ bed slope - t time - u(x, t) mean velocity along the water depth - x distance - Y() cumulative infiltration - (t) distance separating two flow regions - infiltration opportunity time  相似文献   

Hydraulic-hydrological simulations of canal-command for irrigation water management     

R. Singh  J.C. Refsgaard  L. Yde  G.H. Jørgensen  M. Thorsen 《Irrigation and Drainage Systems》1997,11(3):185-213

A modelling system that combines the hydraulic simulations of the canal and hydrological simulations of the irrigated command is introduced. It uses MIKE 11 and MIKE SHE, two well-established modelling systems, for the hydraulic and hydrological simulations respectively. In addition, it also has an irrigation scheduling module and a crop growth module. The modelling system is applied to the Mahanadi Reservoir Irrigation Scheme, a large irrigation project in Central India. The results show that presently a significant amount of water is wasted in the command during the monsoon season. It is demonstrated that the minimization of this wastage could lead to a substantial crop production in the subsequent dry season. Furthermore, the simulations illustrate the versatility of the modelling system for planning and analysing the various aspects of an irrigation project.  相似文献   

Past, present and future criteria to breed crops for water-limited environments in West Africa     

M. Dingkuhn  B.B. Singh  B. Clerget  J. Chantereau  B. Sultan 《Agricultural Water Management》2006,80(1-3):241-261

Asia's Green Revolution of the 1960s and 1970s has largely bypassed West Africa, and “modern” (high-yielding, input responsive) germplasm for staple crops has found comparatively little adoption, except for systems that are have good access to markets and sufficient water resources. It is unlikely, however, that breeding objectives conserving traditional crop characteristics as found in extensive systems would have been more successful. The authors identify systems caught in the agricultural transition from subsistence to intensified, market-oriented production as the most important target for crop improvement, and provide examples of new breeding objectives for cowpea, sorghum and upland rice. In each of these cases, breeders, with the help of physiologists, have developed innovative plant-type concepts that combine improved yield potential and input responsiveness with specific traditional crop characteristics that remain essential during the agricultural transition. In the case of cowpea, dual-purpose varieties were developed that produce a good grain yield due to an erect plant habit, then produce new leaves enabling a second harvest of green foliage. For upland rice systems that are limited by labour (mainly needed to control weeds that abound due to shortened fallow periods), a weed competitive plant type was developed from Oryza sativa × Oryza glaberrima crosses. Lastly, sorghum breeders who had previously deselected photoperiod sensitivity are now re-inserting sensitivity into plants having “modern” architecture, in order to allow for flexible sowing dates while maintaining an agro-ecologically optimal time of flowering near the end of the wet season. The ecophysiological basis of these plant types, their place in current and future cropping systems, as well as the problem of under-funding for their realisation, are discussed.  相似文献