Prostaglandin Synthesis Genes are Differentially Transcripted in Normal and Pyometra Endometria of Bitches     

E Silva  S Leitão  G Ferreira-Dias  L Lopes da Costa  L Mateus 《Reproduction in domestic animals》2009,44(S2):200-203

  相似文献   

Oestrogen and Progesterone Receptor Gene Expression in Canine Oocytes and Cumulus Cells Throughout the Oestrous Cycle     

JSA Gonçalves  CI Vannucchi  FC Braga  FF Paula-Lopes  MP Milazzotto  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2009,44(S2):239-242

  相似文献   

Systemic alterations of bovine hemostasis due to Rhipicephalus (Boophilus) microplus infestation     

Reck J  Berger M  Terra RM  Marks FS  da Silva Vaz I  Guimarães JA  Termignoni C 《Research in veterinary science》2009,86(1):56-198

The tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite that causes considerable economic losses to cattle breeding. Although R. microplus saliva contains several molecules that interfere with the blood coagulation process, so far the systemic alterations in the host hemostatic system have not been described. This study aims to determine if R. microplus infestation induces any disturbance to the host’s hemostatic system. To address these questions, six calves were experimentally infested with 20,000 R. microplus larvae and their blood was collected before and 7, 14 and 21 days post-infestation. Collagen and ADP-induced platelet aggregation as well as coagulation (activated partial thromboplastin time and prothrombin time) decreased in infested bovines. Platelet blood count and fibrinogen increased during the course of infestation, probably as a compensatory response. These alterations may play a role in host health status, and show that the host cannot fully counteract the tick anti-hemostatic mechanisms.  相似文献   

Effects of 12 hour calf withdrawal on conception rate and calf performance of <Emphasis Type="Italic">Bos indicus</Emphasis> cattle under extensive conditions     

R. J. A. Escrivão  E. C. Webb  A. P. J. T. Garcês 《Tropical animal health and production》2009,41(1):135-139

Fifty-two multiparous Brahman type cows with reproductive tract scoring (RTS) ≥4 at 45 days post-partum were randomly assigned to two groups of 26 cows each separated into an ad libitum suckling group (C) and treatment group (T). Calves in the T group were separated for 12 h during the night from 45 days post-partum to the onset of the breeding season. Body condition score (BCS) and body weight (BW) were recorded 45 days post-partum, at the start of the breeding season, and at pregnancy diagnosis. Calves were weighed at calving and weaning. Weaning weights were corrected to 205 days. BW and BCS at the onset of the breeding season were similar (p > 0.05) between the experimental groups. Calving to breeding intervals were 93 ± 18 d and 99 ± 22 d for T and C groups, respectively. Calving to conception intervals differed significantly between the groups (111 ± 10 d for T and 133 ± 19 d for C) and a similar result was obtained for the breeding to conception intervals (18 ± 15 d for T and 31 ± 19 d for C). Conception rates were 80% for the T group and 59% for the C group, which correlated better with BW than BCS at the onset of the breeding season. Weaning weights differed (p < 0.05) between C and T groups. From 45 days post-partum to the onset of the breeding season, cows in the T group experienced a positive energy balance (3%) while those in the C group had a negative energy balance (-0.1%). It was concluded that 12 h calf separation at night increases the conception rates and improves the calf weaning weights of Bos indicus beef cattle under extensive production systems in sub-tropical conditions.  相似文献   

Diagnosis of canine leishmaniasis: conventional and molecular techniques using different tissues     

Maia C  Ramada J  Cristóvão JM  Gonçalves L  Campino L 《Veterinary journal (London, England : 1997)》2009,179(1):142-144

Serology, bone marrow (BM)-, lymph node (LN)- and whole blood-polymerase chain reaction (PCR) were evaluated as potential reference tests for the diagnosis of canine leishmaniasis. A high degree of agreement (91.0%) was observed between Leishmania cultures and serology or BM/LN-PCR. In the light of these results as well as the access to biological test material and the cost of each method, LN-PCR is recommended for the diagnosis or therapeutic control of canine leishmaniasis, but BM-PCR is a suitable alternative in dogs without detectable adenomegaly. For large-scale epidemiological field studies, antibody detection is appropriate and whole blood-PCR can be used to complement the serological results.  相似文献   

Treatment of Waters Containing the Thiocarbamate Herbicide Molinate through an Adsorption/Bio-Regeneration System using a Low-Cost Adsorbent     

Débora Carvalho  Adélio Mendes  Fernão D. Magalhães  Olga C. Nunes 《Water, air, and soil pollution》2010,207(1-4):289-298

The feasibility of using recycled granular tire rubber (GTR) to remove molinate from contaminated water bodies was evaluated in this study. Adsorption equilibrium data was well described by a linear isotherm, and the adsorption was completely reversible. Breakthrough curves showed column efficiencies of approximately 40%, based on total capacity, and complete bed regeneration was achieved using clean water. The effluent from the regeneration step was successfully decontaminated using a defined bacterial mixed culture, capable of molinate mineralization. It was shown that this treated water can be used for regenerating a subsequently saturated bed. The GTR adsorbent showed two important features: complete reversibility towards molinate adsorption and stability along successive adsorption/bio-regeneration cycles. Common adsorbents, such as activated carbons and resins, loose performance very quickly under the same conditions, due to irreversible adsorption.  相似文献   

Cover crops under different managements vs. frequent tillage in almond orchards in semiarid conditions: Effects on soil quality   总被引：1，自引：0，他引：1  

María E. Ramos  Emilio Benítez  Pedro A. García  Ana B. Robles 《Applied soil ecology》2010,44(1):6-14

Frequent tillage has been widely used in rainfed orchards in SE Spain in order to impede weed establishment and to increase water reposition in the soil profile. However, this practice may lead to soil degradation by decreasing structural stability, organic carbon content and microbial activity. This work examines the effect of different cover crop managements and frequent tillage on soil physical, chemical and biological properties in almond orchards in SE Spain. Two cover crops (oat – Avena sativa L. and oat-vetch – Vicia sativa L.) with two fertilization managements (mineral and organic) and three harvesting regimes (grazing in mid May, hay in early June, and grain-straw in mid July) were compared to a frequently tilled orchard system (three or four tillages per year). Most parameters were sensitive to soil management. Wet aggregate stability, total organic carbon, total nitrogen, carbon:nitrogen ratio, phosphatase, and β-glucosidase activities increased with cover crops, whereas the soil–water content declined, especially for the grain-straw treatment. The kind of fertilizer affected the available P content, which was higher for mineral fertilizer, and influenced the β-glucosidase activity, which augmented for the organic fertilizer. Livestock dejections depressed phosphatase activity, and increased WSC and available P.This study suggests that cover crops in semiarid environments improve soil quality compared to frequently tilled management, by increasing the organic matter content, improving the chemical and physical fertility of the soil, and enhancing the soil biological activity. Only higher water extraction by the plants could affect the orchard development and/or productivity; however, early cover crop removal would minimize possible yield losses.  相似文献   

Carbon‐mineralization kinetics in an organically managed Cambic Arenosol amended with organic fertilizers     

Henrique M. Ribeiro  David Fangueiro  Fátima Alves  Ernesto Vasconcelos  João Coutinho  Roland Bol  Fernanda Cabral 《植物养料与土壤学杂志》2010,173(1):39-45

The dynamics of C mineralization in an organically managed Cambic Arenosol amended with hen manure, a stabilized compost (compost), and three different combinations of both fertilizers (varying from a 1:100 to a 1:10 ratio) were studied during an incubation experiment to estimate the potential of such combinations to preserve/restore soil C content relative to single applications. A strong increase of the CO₂‐C emissions relative to the unamended soil (control) was observed after soil application of all five organic‐fertilizer treatments. A significantly higher amount of applied C was lost in hen‐manure treatment (648 mg CO₂‐C [g C applied]^–1) when compared to compost (159 mg CO₂‐C [g C applied]^–1) or to the three combined treatments (176–195 mg CO₂‐C [g C applied]^–1). The first‐order exponential model and the double exponential model were used to fit the C‐mineralization data in the treatments considered. Results showed that mixing “small” amounts of hen manure with compost did not affect the total amount of potentially mineralizable C, but significantly increased the mineralization rate constant. Clearly, combinations of both fertilizers promoted an initial faster mineralization of the organic matter, and consequently a faster release of nutrients, without affecting the total amount of C sequestered in soil.  相似文献   

Higher breeding densities of the threatened little bustard Tetrax tetrax occur in larger grassland fields: Implications for conservation     

João Paulo Silva  Jorge M. Palmeirim 《Biological conservation》2010,143(11):2553-2558

Previous studies have found that densities of little bustard Tetrax tetrax breeding males tend to be higher in areas with smaller agricultural fields, presumably due to increased habitat diversity. However, exceptionally high densities have been found in large grassland fields in Portugal, which suggests that the influence of field size varies geographically, and that the role of this factor is not yet fully understood, despite its importance as a key management issue.We studied how field size, together with vegetation structure, influences the presence and density of breeding little bustards in a region of southern Portugal. Fifty-four grassland fields were sampled in 2007 and another 29 in 2008, with sizes ranging from 23 to 172 ha. A total of 183 breeding males were found in 47 of these fields, reaching densities of up to 37 males/100 ha. A higher probability of occurrence of breeding males was found in larger fields with a vegetation height below 40 cm and field size alone explained 46% of the variability in male density. These results suggest that larger continuous areas of suitable habitat attract many males, most likely as a consequence of their lek mating system. We conclude that conservation efforts, in a landscape context of large farm sizes, should: (1) be channelled to farms with large fields; (2) ensure adequate livestock grazing to create suitable habitat and (3) promote management at a landscape level to ensure the most continuous grassland habitat patches possible.  相似文献   

Life cycle and reproductive traits of the earthworm Aporrectodea trapezoides (Dugès, 1828) in laboratory cultures     

Rosa Fernández  Marta Novo  Mónica Gutiérrez  Ana Almodóvar  Darío J. Díaz Cosín 《Pedobiologia》2010,53(5):295-290

The parthenogenetic earthworm Aporrectodea trapezoides (Dugès, 1828) is widely distributed all over the world due to European agricultural practices. In order to provide baseline life cycle data, cocoons were obtained from field-collected individuals and their features and viability, incubation period, number of hatchlings and mortality rate were recorded. Singleton and twin earthworms from this first experiment were cultured from hatching during a 490-day period under controlled conditions with biomass, survival, reproductive condition and cocoon production recorded at intervals of 15 days. On average, individuals of isolated-reared A. trapezoides reached maturity at day 153 and body weight at maturity was approximately 1 g. In order to record reproductive traits and differences between field-collected and laboratory-reared individuals, 40 microcosms with an isolated earthworm (20 with field-collected individuals and 20 with laboratory-reared ones) and 40 containing groups of three (20 with each type of individual) were maintained during a complete year under controlled conditions. The amount of soil per individual was the same in both types of microcosm. Both the individuals kept in isolation and those cultured in groups produced cocoons, hence completely proving the obligatory parthenogenetic reproduction in this species without copulation or need of any physical-chemical stimulus. In general, isolated earthworms produced a significantly higher number of cocoons than those in groups of three, and the same was recorded for laboratory-reared earthworms when compared with field-collected ones. This study highlights the importance of knowing the life cycle and reproductive traits of one possible key species in soil management due to its vast distribution and high density in soils, and the species’ highly recommended use in applied studies because of its ease of culture.  相似文献