Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization   总被引：78，自引：0，他引：78  

M Jaye  R Howk  W Burgess  G A Ricca  I M Chiu  M W Ravera  S J O'Brien  W S Modi  T Maciag  W N Drohan 《Science (New York, N.Y.)》1986,233(4763):541-545

Several of the endothelial cell polypeptide mitogens that have been described probably play a role in blood vessel homeostasis. Two overlapping complementary DNA clones encoding human endothelial cell growth factor (ECGF) were isolated from a human brain stem complementary DNA library. Southern blot analysis suggested that there is a single copy of the ECGF gene and that it maps to human chromosome 5 at bands 5q31.3 to 33.2 A 4.8-kilobase messenger RNA was present in human brain stem messenger RNA. The complete amino acid sequence of human ECGF was deduced from the nucleic acid sequence of these clones; it encompasses all the well-characterized acidic endothelial cell polypeptide mitogens described by several laboratories. The ECGF-encoding open reading frame is flanked by translation stop codons and provides no signal peptide or internal hydrophobic domain for the secretion of ECGF. This property is shared by human interleukin-1, which is approximately 30 percent homologous to ECGF.  相似文献   

Human sperm binding is mediated by the sialyl-Lewis(x) oligosaccharide on the zona pellucida     

Pang PC  Chiu PC  Lee CL  Chang LY  Panico M  Morris HR  Haslam SM  Khoo KH  Clark GF  Yeung WS  Dell A 《Science (New York, N.Y.)》2011,333(6050):1761-1764

Human fertilization begins when spermatozoa bind to the extracellular matrix coating of the oocyte, known as the zona pellucida (ZP). One spermatozoan then penetrates this matrix and fuses with the egg cell, generating a zygote. Although carbohydrate sequences on the ZP have been implicated in sperm binding, the nature of the ligand was unknown. Here, ultrasensitive mass spectrometric analyses revealed that the sialyl-Lewis(x) sequence [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc], a well-known selectin ligand, is the most abundant terminal sequence on the N- and O-glycans of human ZP. Sperm-ZP binding was largely inhibited by glycoconjugates terminated with sialyl-Lewis(x) sequences or by antibodies directed against this sequence. Thus, the sialyl-Lewis(x) sequence represents the major carbohydrate ligand for human sperm-egg binding.  相似文献   

Interleukin-1 costimulatory activity on the interleukin-2 promoter via AP-1   总被引：37，自引：0，他引：37  

K Muegge  T M Williams  J Kant  M Karin  R Chiu  A Schmidt  U Siebenlist  H A Young  S K Durum 《Science (New York, N.Y.)》1989,246(4927):249-251

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Neuroleptic drug-induced dopamine receptor supersensitivity: antagonism by L-prolyl-L-leucyl-glycinamide   总被引：1，自引：0，他引：1  

S Chiu  C S Paulose  R K Mishra 《Science (New York, N.Y.)》1981,214(4526):1261-1262

An animal model of tardive dyskinesia was used to evaluate the potential antidyskinetic properties of the neuropeptide L-prolyl-L-leucyl-glycinamide (PLG). In rats, PLG administered concurrently with the neuroleptic drug haloperidol or chlorpromazine antagonized the enhancement of specific [3H]spiroperidol binding in the striatum that is associated with long-term neuroleptic treatment. The results are discussed in relation to a possible functional coupling of the putative PLG receptor with neuroleptic-dopamine receptor complex and clinical implications for tardive dyskinesia.  相似文献   

The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans     

Loftus BJ  Fung E  Roncaglia P  Rowley D  Amedeo P  Bruno D  Vamathevan J  Miranda M  Anderson IJ  Fraser JA  Allen JE  Bosdet IE  Brent MR  Chiu R  Doering TL  Donlin MJ  D'Souza CA  Fox DS  Grinberg V  Fu J  Fukushima M  Haas BJ  Huang JC  Janbon G  Jones SJ  Koo HL  Krzywinski MI  Kwon-Chung JK  Lengeler KB  Maiti R  Marra MA  Marra RE  Mathewson CA  Mitchell TG  Pertea M  Riggs FR  Salzberg SL  Schein JE  Shvartsbeyn A  Shin H  Shumway M  Specht CA  Suh BB  Tenney A  Utterback TR  Wickes BL  Wortman JR  Wye NH  Kronstad JW  Lodge JK 《Science (New York, N.Y.)》2005,307(5713):1321-1324

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精油防治仓库害虫的实仓应用   总被引：5，自引：0，他引：5  

徐汉虹  江福银 《华南农业大学学报》1993,14(3):42-47

采用4种精油对4种仓库害虫进行了模拟实仓防治试验.结果发现,0.2％(W/W)肉桂油拌种对小麦的保护效果可维持8个月以上.以0.45mg/cm~2的剂量处理麻袋,齿叶黄皮和八角茴香精油处理能保持小麦4个月无虫,肉桂油可保持8个月无虫.实仓试验中发现,15ppm马拉硫磷加15ppm肉桂油混用处理.虫口减退率达100％.30ppm马拉硫磷和30ppm肉桂油处理的虫口减退率分别为89.8％和98.5％.  相似文献   

印楝引种试验初报   总被引：40，自引：2，他引：40  

赵善欢  蔡德智 《华南农业大学学报》1989,10(2):34-39

1986年在我国海南省万宁县首次引种印楝(Azadirachta indica A.Juss)的初步结果表明,印楝树适宜在该地种植,在没有采取特殊保护措施的自然条件下能顺利越冬越夏,生长情况良好。育苗三个月即可移植出圃造林。移植两年后可郁闭成林。平均株高可达6米,胸径达9.8厘米。树龄两年即出蕾开花,首次花期在4～5月份。同年8～9月份果实成熟,产生的种子具有正常生活力。据试验期间观察,印楝还具有较强的抗风和耐旱能力,并具有一定的抗寒能力,是值得进行大量推广的速生树种。  相似文献   

Immunolocalization of chloride transporters to gill epithelia of euryhaline teleosts with opposite salinity-induced Na<Superscript>+</Superscript>/K<Superscript>+</Superscript>-ATPase responses     

Tang CH  Hwang LY  Shen ID  Chiu YH  Lee TH 《Fish physiology and biochemistry》2011,37(4):709-724

Opposite patterns of branchial Na⁺/K⁺-ATPase (NKA) responses were found in euryhaline milkfish (Chanos chanos) and pufferfish (Tetraodon nigroviridis) upon salinity challenge. Because the electrochemical gradient established by NKA is thought to be the driving force for transcellular Cl⁻ transport in fish gills, the aim of this study was to explore whether the differential patterns of NKA responses found in milkfish and pufferfish would lead to distinct distribution of Cl⁻ transporters in their gill epithelial cells indicating different Cl⁻ transport mechanisms. In this study, immunolocalization of various Cl⁻ transport proteins, including Na⁺/K⁺/2Cl⁻ cotransporter (NKCC), cystic fibrosis transmembrane conductance regulator (CFTR), anion exchanger 1 (AE1), and chloride channel 3 (ClC-3), were double stained with NKA, the basolateral marker of branchial mitochondrion-rich cells (MRCs), to reveal the localization of these transporter proteins in gill MRC of FW- or SW-acclimated milkfish and pufferfish. Confocal microscopic observations showed that the localization of these transport proteins in the gill MRCs of the two studied species were similar. However, the number of gill NKA-immunoreactive (IR) cells in milkfish and pufferfish exhibited to vary with environmental salinities. An increase in the number of NKA-IR cells should lead to the elevation of NKA activity in FW milkfish and SW pufferfish. Taken together, the opposite branchial NKA responses observed in milkfish and pufferfish upon salinity challenge could be attributed to alterations in the number of NKA-IR cells. Furthermore, the localization of these Cl⁻ transporters in gill MRCs of the two studied species was identical. It depicted the two studied euryhaline species possess the similar Cl⁻ transport mechanisms in gills.  相似文献   

Schisandrin B protects against solar irradiation-induced oxidative stress in rat skin tissue     

Lam PY  Yan CW  Chiu PY  Leung HY  Ko KM 《Fitoterapia》2011,82(3):393-400

Schisandrin B (Sch B) and schisandrin C (Sch C), but not schisandrin A and dimethyl diphenyl bicarboxylate, protected rat skin tissue against solar irradiation-induced oxidative injury, as evidenced by a reversal of solar irradiation-induced changes in cellular reduced glutathione and α-tocopherol levels, as well as antioxidant enzyme activities and malondialdehyde production. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production in rat skin microsomes. Taken together, Sch B or Sch C, by virtue of its pro-oxidant action and the subsequent eliciting of a glutathione antioxidant response, may prevent photo-aging of skin.  相似文献   

Seasonal changes in atrophy-associated proteins of the sonic muscle in the big-snout croaker, Johnius macrorhynus (Pisces, Sciaenidae), identified by using a proteomic approach     

Lin YC  Chiu KH  Shiea J  Huang HW  Mok HK 《Fish physiology and biochemistry》2011,37(4):977-991

In most sciaenids, males possess sonic muscles and produce sound through the contraction of these muscles and amplification of the swim bladder. The sonic muscles in some fishes exhibit seasonal changes in size. For example, they are hypertrophic in the spawning season, and atrophic in the non-spawning months. The protein profiles of the sonic muscle, red muscle, and white muscle in the Johnius macrorhynus were shown by two-dimensional electrophoresis (2-DE) and were compared to reveal differential protein expressions. About 80 up-regulated protein spots in the sonic muscle, and 30 spots related to six contractile proteins (fast muscle myosin heavy chain, skeletal alpha actin, alpha actin cardiac, tropomyosin, myosin light chain 2, and myosin light chain 3), four energy metabolic enzymes (enolase, acyl-CoA synthetase, creatine kinase, and cytochrome P450 monooxygenase), and two miscellaneous proteins (DEAD box protein and cyclin H) were identified. Seasonal hypertrophy and atrophy of the sonic muscles related to the reproductive cycle were verified in male big-snout croaker. The contents of some proteins were significantly different in the muscles under these conditions. The levels of cytochrome P450 monooxygenase, fast muscle myosin heavy chain, DEAD box proteins, isocitrate dehydrogenase, and creatine kinase were up-regulated in the hypertrophic muscle, but the levels of alpha actin cardiac, myosin light 2, and myosin light 3 were lower than in the atrophic muscle. Potential reasons for these differences in protein expression related to physiological adaptation are discussed.  相似文献