农用钴室的扩建及环境监测     

哈益明  牛传堂 《山东农业大学学报(自然科学版)》1992,23(4):353-357

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Evaluation of a bar-code system for nutrient analysis in dietary surveys     

Anderson AS  Maher L  Ha TK  Cooney J  Eley S  Martin M  Vespasiani G  Bruni M  Lean ME 《Public health nutrition》1999,2(4):579-586

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Effect of EDTA alone and in combination with polygodial on the growth of Saccharomyces cerevisiae     

Kubo I  Lee SH  Ha TJ 《Journal of agricultural and food chemistry》2005,53(5):1818-1822

The antifungal activity of ethylenediaminetetraacetic acid (EDTA) against Saccharomyces cerevisiae was significantly affected by various conditions such as inoculum size, pH, and metal ions (Mg(2+), Ca(2+)). EDTA was found to be effective against this yeast at the inoculum size of 10(5) colony forming units (CFU)/mL with the minimum inhibitory concentration of 400 mug/mL and the minimum fungicidal concentration of 6400 mug/mL, but it was not effective at 10(7) CFU/mL up to 6400 mug/mL. The fungicidal activity of EDTA against S. cerevisiae was significantly enhanced in combination with polygodial. Isobolograms, fractional inhibitory concentration, and fractional fungicidal concentration indices were used for evaluating the interaction between combined compounds. This synergistic effect is likely due to polygodial's destructive action on the cellular membrane, which facilitates the transmembrane transport of foreign compounds (EDTA) into yeast cells. Once inside the cells, EDTA forms chelation with divalent metals such as Mg(2+) and Ca(2+), which are required by various essential enzymes.  相似文献   

Single nucleotide polymorphism discovery and linkage mapping of lipoxygenase-2 gene (Lx 2) in soybean     

Moon Young Kim  Bo-Keun Ha  Tae-Hwan Jun  Eun-Young Hwang  Kyujung Van  Yong In Kuk  Suk-Ha Lee 《Euphytica》2004,135(2):169-177

Lipoxygenase-2 (Lx ₂) in soybean seed is mainly responsible for generation of grassy-beany and bitter flavors. Genetic elimination of this flavor can be accelerated by the development of single nucleotide polymorphism (SNP) markers linked to Lx ₂. A frame map based on simple sequence repeat (SSR) markers was constructed first using a recombinant inbred line (RIL) population of Pureunkong × Jinpumkong 2. Sixty-five SSR markers were incorporated into 13 linkage groups (LGs) spanning a total of 737 cM. Among five primer pairs designed from the Lx ₂ gene sequence, one produced an amplicon with sequence variations between Pureunkong and Jinpumkong 2. Three SNPs, T/C, G/A and C/A, were identified at 251,367 and 420 bp, respectively, in the intron region of the 804 bp amplified product. Using single base chain extension based on the capture probe sequence in the 5' region of the T/C SNP, the 90 RILs were genotyped for each allele of Lx ₂. The allelic segregation for the SNP linked toLx ₂ was in accordance with the expected ratio of 1:1 in the RIL population. Based on the results of linkage analysis between Lx ₂ and the SSR markers, Lx ₂ was found to be positioned on one end of LG F in the frame map, flanked by the SSR markers Satt522 and Sat074. This study demonstrates that SNP markers closely linked to Lx ₂can be developed to facilitate marker-assisted selection and fine mapping of the region around this locus. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Comparison of a high oleic acid soybean line to cultivated cultivars for seed yield,protein and oil concentrations     

Hyun-Jee Kim  Bo-Keun Ha  Keon-Soo Ha  Jong-Hyun Chae  Ji-Ho Park  Min-Su Kim  Sovetgul Asekova  J. Grover Shannon  Chang-Ki Son  Jeong-Dong Lee 《Euphytica》2015,201(2):285-292

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新疆额敏县野果林苹果巢蛾主要寄生蜂调查   总被引：1，自引：0，他引：1  

哈米提  姚艳霞  赵文霞  淮稳霞 《中国生物防治》2011,27(1):128-131

近年来,新疆塔城地区额敏县野果林遭受苹果巢蛾Yponomeuta padella Linnaeus的严重危害,造成重大经济损失.为寻找其有效天敌,保护这些珍贵的果树资源,2008-2009年,对额敏县野果林苹果巢蛾的寄生性天敌资源展开了调查.通过野外采集及室内饲养,发现了寄生于该害虫卵、幼虫及蛹的3种寄生蜂,即:卵-幼虫跨期寄生蜂--巢蛾多胚跳小蜂Ageniaspis fuscicollis(Dalman),蛹期寄生蜂-黄柄齿腿长尾小蜂Monodontomerusdentipes(Dalman)和幼虫-蛹跨期寄生蜂--全北群瘤姬蜂指名亚种Iseropus stercorator stercorator(Fabricius);其中巢蛾多胚跳小蜂为优势种,寄生率可达95.98%.本研究为苹果巢蛾的生物防治奠定了基础.  相似文献   

Biotransformation and impact of ferulic acid on phenylpropanoid and capsaicin levels in Capsicum annuum L. cv. P1482 cell suspension cultures     

Kang SM  Jung HY  Kang YM  Min JY  Karigar CS  Yang JK  Kim SW  Ha YR  Lee SH  Choi MS 《Journal of agricultural and food chemistry》2005,53(9):3449-3453

Cell suspension cultures of Capsicum annuum L. cv. P1482 were fed with exogenous ferulic acid to monitor their biotransformation abilities. A portion of the ferulic acid was biotransformed into vanillin, a major natural flavor, and capsaicin, a principle secondary metabolite characteristic of Capsicum species. The cellular vanillin concentrations were relatively higher than capsaicin levels and were maximal (2 mg/g DW) 4 days after 0.6 mM ferulic acid feeding. Maximal vanillin levels in the culture medium were 10 mg/L at 4 and 3 days after feeding with 1.25 and 2.5 mM ferulic acid, respectively. With regard to capsaicin levels, the cellular levels were slightly decreased by ferulic acid feeding, whereas the levels in the culture medium were increased. Ferulic acid feeding not only enhanced vanillin and capsaicin production but also increased the concentrations of other phenylpropanoid metabolites.  相似文献   

The impact of a school-based nutrition education intervention on dietary intake and cognitive and attitudinal variables relating to fruits and vegetables     

Anderson AS  Porteous LE  Foster E  Higgins C  Stead M  Hetherington M  Ha MA  Adamson AJ 《Public health nutrition》2005,8(6):650-656

OBJECTIVE: To assess the impact of a school-based nutrition education intervention aimed at increasing the consumption of fruits and vegetables. DESIGN: The intervention programme increased the provision of fruits and vegetables in schools and provided a range of point-of-purchase marketing materials, newsletters for children and parents, and teacher information. Curriculum materials at age 6-7 and 10-11 years were also developed and utilised. Evaluation was undertaken with groups of younger (aged 6-7 years) and older (aged 10-11 years) children. Methods included 3-day dietary records with interview and cognitive and attitudinal measures at baseline, with follow-up at 9 months, in intervention and control schools. SETTING: The work was undertaken in primary schools in Dundee, Scotland. SUBJECTS: Subjects comprised 511 children in two intervention schools with a further 464 children from two schools acting as controls. RESULTS: Children (n=64) in the intervention schools had an average increase in fruit intake (133+/-1.9 to 183+/-17.0 g day(-1)) that was significantly (P<0.05) greater than the increase (100+/-11.7 to 107+/-14.2 g day(-1)) estimated in children (n=65) in control schools. No other changes in food or nutrient intake were detected. Increases in scores for variables relating to knowledge about fruits and vegetables and subjective norms were also greater in the intervention than in the control group, although taste preferences for fruits and vegetables were unchanged. CONCLUSIONS: It is concluded that a whole school approach to increasing intakes of fruits and vegetables has a modest but significant effect on cognitive and attitudinal variables and on fruit intake.  相似文献   

Rice tungro spherical virus resistance into photoperiod-insensitive japonica rice by marker-assisted selection     

Junghyun Shim  Gideon Torollo  Rosalyn B. Angeles-Shim  Rogelio C. Cabunagan  Il-Ryong Choi  Un-Sang Yeo  Woon-Goo Ha 《Breeding Science》2015,65(4):345-351

Rice tungro disease (RTD) is one of the destructive and prevalent diseases in the tropical region. RTD is caused by Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Cultivation of japonica rice (Oryza sativa L. ssp japonica) in tropical Asia has often been restricted because most japonica cultivars are sensitive to short photoperiod, which is characteristic of tropical conditions. Japonica1, a rice variety bred for tropical conditions, is photoperiod-insensitive, has a high yield potential, but is susceptible to RTD and has poor grain quality. To transfer RTD resistance into Japonica1, we made two backcrosses (BC) and 8 three-way crosses (3-WC) among Japonica1 and RTSV-resistant cultivars. Among 8,876 BC₁F₂ and 3-WCF₂ plants, 342 were selected for photoperiod-insensitivity and good grain quality. Photoperiod-insensitive progenies were evaluated for RTSV resistance by a bioassay and marker-assisted selection (MAS), and 22 BC₁F₇ and 3-WCF₇ lines were selected based on the results of an observational yield trial. The results demonstrated that conventional selection for photoperiod-insensitivity and MAS for RTSV resistance can greatly facilitate the development of japonica rice that is suitable for cultivation in tropical Asia.  相似文献   

Expression of mucins on the mucosal surface of lungs of 4-week-old pigs     

Kim CH  Lee K  Han K  Oh Y  Kim D  Seo HW  Park C  Ha MK  Kim SH  Cho KD  Lee BH  Chae C 《Veterinary research communications》2011,35(4):255-260

The aim of this study was to determine the immunoreactivity of normal small bronchial, bronchiolar, respiratory bronchiolar, and interalveolar epithelium using antibodies to six mucins: MUC1, MUC2, MUC4, MUC5AC, MUC5B, and MUC6. The large, gel-forming secreted mucins MUC2, MUC5AC, and MUC5B were widely expressed in the lower respiratory tract. The results of this study demonstrate that these secreted mucins form a gel to cover and protect the mucosal surface in the lower respiratory tract of pigs.  相似文献