Characterisation of taro (Colocasia esculenta (L.) Schott) genetic resources in Southeast Asia and Oceania     

V. Lebot  M.S. Prana  N. Kreike  H. van Heck  J. Pardales  T. Okpul  T. Gendua  M. Thongjiem  H. Hue  N. Viet  T.C. Yap 《Genetic Resources and Crop Evolution》2004,51(4):381-392

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Methylation methods for the quantitative analysis of conjugated linoleic acid (CLA) isomers in various lipid samples   总被引：8，自引：0，他引：8  

Park SJ  Park CW  Kim SJ  Kim JK  Kim YR  Park KA  Kim JO  Ha YL 《Journal of agricultural and food chemistry》2002,50(5):989-996

Precise methylation methods for various chemical forms of conjugated linoleic acid (CLA), which minimize the formation of t,t isomers and allylmethoxy derivatives (AMD) with the completion of methylation, were developed using a 50 mg lipid sample, 3 mL of 1.0 N H(2)SO(4)/methanol, and/or 3 mL of 20% tetramethylguanidine (TMG)/methanol solution(s). Free CLA (FCLA) was methylated with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). CLA esterified in safflower oil (CLA-SO) was methylated with 20% TMG/methanol (100 degrees C, 5 min), whereas CLA esterified in phospholipid (CLA-PL) was methylated with 20% TMG/methanol (100 degrees C, 10 min), followed by an additional reaction with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). Similarly, CLA esterified in egg yolk lipid (CLA-EYL) was methylated by base hydrolysis, followed by reaction with 1.0 N H(2)SO(4)/methanol (55 degrees C, 5 min). These results suggest that for the quantitative analysis of CLA in lipid samples by GC, proper methylation methods should be chosen on the basis of the chemical forms of CLA in samples.  相似文献   

Anthropogenic Input of Selected Heavy Metals (Cu, Cr, Pb, Zn and Cd) in the Aquatic Sediments of Hochiminh City, Vietnam     

Hoang Thi Thanh Thuy  Nguyen Nhu Ha Vy  Tu Thi Cam Loan 《Water, air, and soil pollution》2007,182(1-4):73-81

The aquatic system of Hochiminh City comprises two main rivers: the Sai Gon and Nha Be rivers. Five canals discharge into these two rivers: NhieuLoc-ThiNghe, TauHu-BenNghe, TanHoa-LoGom, ThamLuong-BenCat and Doi-Te. The rivers and these canals collect effluent water from domestic and industrial sources. Most of these flows are not treated or at most are only primarily treated. A total of 33 sediment cores were taken from these rivers and canals. Chemical composition of these aquatic sediments has very high concentrations of several “urban” metals such as Cd, Cr, Cu and Zn. Most of the samples have exceeded the US EPA’s toxicity reference values for Cu, Zn and Cr (82, 82 and 70%, respectively). The highest concentrations of these metals appear to be associated with the uncontrolled and untreated industrial runoff to the discharge canals. These concentrations in fluvial sediment are relatively low, which indicates the dilution process of the contaminants. This finding indicates that the anthropogenic inputs play an important role in the elevation of heavy metals in the aquatic system and organic matter seems to exert a strong geochemical control on the amount of heavy metals. The Pearson correlation coefficients calculated for Cd, Cr, Cu and Zn, are 0.89; 0.72; 0.93 and 0.87, respectively.  相似文献   

Outbreak of salmonellosis in pigs with postweaning multisystemic wasting syndrome     

Ha Y  Jung K  Kim J  Choi C  Chae C 《The Veterinary record》2005,156(18):583-584

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Development of nested polymerase chain reaction for the detection of Mycoplasma hyopneumoniae in formalin-fixed paraffin-embedded lung tissue     

Ha SK  Choi C  Kim O  Song HC  Lim ES  Kim SH  Hwang KK  Chae C 《Australian veterinary journal》2005,83(7):442-444

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Development of an optimized protocol for the detection of classical swine fever virus in formalin-fixed, paraffin-embedded tissues by seminested reverse transcription-polymerase chain reaction and comparison with in situ hybridization   总被引：1，自引：0，他引：1  

Ha SK  Choi C  Chae C 《Research in veterinary science》2004,77(2):163-169

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Detection and isolation of H1N1 influenza virus from pigs in Korea   总被引：5，自引：0，他引：5  

Choi C  Ha SK  Chae C 《The Veterinary record》2004,154(9):274-275

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Expression of constitutive endothelial, neuronal and inducible nitric oxide synthase in the testis and epididymis of horse     

Ha TY  Kim HS  Shin T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):351-356

The expression of three isoforms of nitric oxide synthase (NOS) were examined in the testis and epididymis of a thoroughbred horse. Immunohistochemical studies demonstrated the presence of eNOS immunostaining in some germ cells in the seminiferous tubules and in vascular endothelial cells in the interstitial tissues. Interstitial cells, most likely Leydig cells, were also intensely immunopositive for eNOS. The pattern of immunostaining for nNOS was similar to that for eNOS in the testis. Weak expression of iNOS was detected in the seminiferous tubules of the testis, but intense expression was found in interstitial cells. Inducible NOS was also strongly detected in stereocilia, sperm, epithelium and connective tissue of the epididymis of normal horses. These findings suggest that three isoforms of NOS are expressed in the testis and epididymis of horse and that they play important roles in the biology of interstitial cells that produce testosterone, as well as in spermatogenesis in the seminiferous tubules.  相似文献   

Genotypic prevalence of the adhesin involved in diffuse adherence in Escherichia coli isolates in pre-weaned pigs with diarrhoea in Korea   总被引：1，自引：0，他引：1  

Ha SK  Choi C  Jung K  Kim J  Han DU  Ha Y  Lee SD  Kim SH  Chae C 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2004,51(4):166-168

A total of 1002 Escherichia coli strains isolated from pre-weaned pigs with diarrhoea on 1114 swine farms were screened for the presence of the adhesin involved in diffuse adherence (AIDA) gene by polymerase chain reaction (PCR). Escherichia coli isolates that carried AIDA genes were also tested by PCR for the detection of five fimbriae (F4, F5, F6, F18 and F41), heat-stable (STa, STb) and heat-labile (LT) enterotoxin, enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1), and Shiga toxin 2 oedema disease (Stx2e) genes. Twenty-three (2.3%) of the 1002 E. coli isolates carried the gene for AIDA. Among 23 isolates shown to carry genes for AIDA, three carried the AIDA gene as the only shown virulence factor. Other isolates carried other virulence factor genes in addition to AIDA. Four isolates carried genes for at least one of the fimbrial adhesins and enterotoxins. Sixteen isolates carried genes for enterotoxins only. The AIDA may represent an additional virulence determinant in pre-weaned pigs with diarrhoea.  相似文献   

Growth and wood basic density of acacia hybrid clones at three locations in Vietnam   总被引：1，自引：0，他引：1  

Le Dinh Kha  Chris E. Harwood  Nguyen Duc Kien  Brian S. Baltunis  Nguyen Dinh Hai  Ha Huy Thinh 《New Forests》2012,43(1):13-29

Field trials testing a total of 27 clones of the interspecific hybrid Acacia mangium × A. auriculiformis and seedling controls of the parental species were established at Ba Vi and Yen Thanh in the north of Vietnam and Long Thanh in the south. At both Ba Vi and Yen Thanh there were significant (P < 0.001) differences in height and diameter at breast height (DBH) among 22 tested clones at 4 years. At Long Thanh, twelve hybrid clones did not differ significantly in DBH at age 3 years, but did (P < 0.001) at age 5 years. At the two northern sites the acacia hybrid clones had significantly greater DBH than control seedlots of the parental species. At Long Thanh, DBH of the hybrid clones and A. mangium was similar, with a genetically improved seedlot of A. mangium displaying the best DBH. Mean wood basic density at breast height of the acacia hybrid clones was 539 kg m⁻³ at Yen Thanh at age 8 years, and 473 kg m⁻³ at Long Thanh at age 5 years; density for A. mangium at Long Thanh was only slightly lower than the hybrid clones at 461 kg m⁻³. Linear regressions of Pilodyn penetration (PP) at breast height on wood basic density explained 60% of the variance in density of treatments (clones and control seedlots) at Yen Thanh and 36% at Long Thanh. There were significant differences between hybrid clones in PP at all three trial sites. Clonal DBH performance was not strongly correlated across the three trial sites; Pearson correlations of clone mean DBH between pairs of sites ranged from −0.47 to 0.20. Clonal rankings for PP were more stable, with Pearson correlations between pairs of sites ranging from r = 0.71 to 0.78.  相似文献