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201.
A steam-dryer, designed and manufactured in Sweden, was evaluated for destroying Bursaphelenchus xylophilus in pine chips from southern USA. In a trial, pine chips were treated at different temperature and pressure regimes for 5 or 10min. The lowest time/temperature/pressure combination was to increase the vessel temperature from 85 to 104°C in 5 min at low pressure. Samples of pine chips were assayed for nematodes in laboratories in USA, Sweden, and Finland. No nematodes of any species were recovered from any of the treated pine-chip samples. Large and heterogeneous pieces of wood in the treated samples were also nematode-free. The apparatus was a prototype capable of handling about 400 kg h-1. The final version would have to be able to process 100-200 th-1.  相似文献   
202.
Antiserum to a partially purified neuraminidase fromPasteurella multocida, type A:3, was adsorbed with protease-digestedP. multocida type 3 lipopolysaccharide (LPS) to remove LPS immunoreactivity. The LPS-adsorbed antineuraminidase caused a 77% reduction in the neuraminidase activity of homologousP. multocida in anin vitro enzyme neutralization test. All 14 mice passively immunized with the adsorbed antineuraminidase were protected against challenge infection with homologousP. multocida in a mouse protection test. Ten out of 14 mice in one group that received antisera containing antibodies to both neuraminidase and LPS were protected. In contrast, only 1 out of 14 mice that were immunized with pre-immune serum survived the challenge. These results suggest that antiserum toP. multocida neuraminidase was, at least partly, responsible for the protection observed in this study. Neuraminidase may be one of the immunogenic protective proteins present in aqueous extracts ofPasteurella multocida.  相似文献   
203.
204.
Hyperchylomicronaemia was identified in a four-week-old Siamese kitten with lethargy, in-appetence, hindlimb ataxia and profound anaemia. The kitten was euthanased and at necropsy a thrombus was found occluding the caudal aorta. Two littermates later presented with lethargy, inappetence and hypertriglyceri-daemia which resolved after being weaned on to a low fat diet. A similar condition was subsequently diagnosed in a kitten born to the same sire but a different queen. The expression of hyperchylomicronaemia in two related litters was suggestive of an inherited, familial defect in the function of lipoprotein lipase (LPL). The activity of this enzyme was reduced in all three parents, the two recovered cases and two related, but apparently unaffected kittens, compared with a control group of unrelated cats belonging to the breeder. This reduction in activity was not attributable to defective activation of LPL by its serum cofactor apolipoprotein C-II or the presence in plasma of a factor that inhibited LPL. The gene that codes for LPL was examined by restriction fragment length polymorphism analysis using a human LPL cDNA probe. The results showed that the cat has a similar, but not identical, LPL gene to man. However, there were no differences in the restriction fragment patterns obtained from affected, unaffected and control animals.  相似文献   
205.
206.
Estrogen and progesterone receptors (ER, PR) were measured in cytosol fractions from 18 primary canine mammary carcinomas by use of biochemical assays. One or both receptors were detected (> 10 fmol/mg of cytosol protein) in 11 tumors: 5 ER and PR; 2 ER only; 4 PR only. Mean cytoplasmic receptor concentrations (fmol/mg of cytosol protein) were 22.8 +/- 2.9 (SEM) for ER and 51.0 +/- 10.3 for PR in tumors containing ER and PR, 28.8 +/- 12.1 for ER in tumors containing only ER and 13.2 +/- 1.5 for PR in tumors containing only PR. Estrogen or progesterone receptors or both were identified in 6 of 9 tubular adenocarcinomas, 4 of 5 papillary adenocarcinomas, and 1 of 1 squamous cell carcinoma. These receptors were not identified in solid carcinomas (n = 2) or a single spindle cell carcinoma. Although the number of cases was limited, survival times of dogs tended to be longest in those with tumors containing ER alone or in combination with PR, intermediate in those with tumors containing only PR, and shortest in those with tumors without ER or PR. A correlation was not apparent between receptor status and age, presence of ovaries, tumor size, or histologic classification of the tumor. In the analysis of this series, the extent of surgery (mastectomy of the involved gland vs unilateral or bilateral mastectomy) did not appear to influence the outcome of the disease, and metastasis to regional lymph nodes did not appear to be a reliable prognostic indicator.  相似文献   
207.
Rostral and caudal rhinoscopy in dogs and cats facilitates the investigation of the nasal cavity and accurate biopsy. Rostral rhinoscopy can be performed by rigid endoscopes; caudal rhinoscopy requires flexible endoscopes. Deep anaesthesia or additional analgesia with local anaesthesia is necessary. The nasal cavity is assessed by its form, colour, surface of the mucous membrane, hyperemia, plaques, lesions, and the secretion is assessed by its quantity, colour and viscosity. Foreign bodies and neoplasia must also be looked for. Case reports with abnormal findings are described.  相似文献   
208.
Bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. Two strains of bluetongue virus serotype 11 designated as UC-2 and UC-8 have different virulence patterns in newborn mice. These viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. Four bovine fetuses each were inoculated intramuscularly with either UC-2 or UC-8, and one fetus was inoculated with placebo. The inoculation was made intramuscularly through the uterine wall at 120 days' gestation, and the bovine fetuses were recovered by cesarean section 12 or 20 days after inoculation. Fetal blood was collected for virus isolation and serology. Virus was reisolated from brain, blood, lung and liver. Both strains, UC-2 and UC-8, cause severe lesions in the 120 day fetuses. The encephalomalacic lesions occurred earlier and were more severe in fetuses inoculated with UC-8 as compared to those inoculated with UC-2. The subtle differences observed in the fetuses inoculated with the two different strains suggest that there is a difference in pathogenic potential of the two viruses. These differences do not appear to be completely dependent upon the host species.  相似文献   
209.
Fetal amino acid nutrition and metabolism have been studied primarily in pregnant sheep. The umbilical uptake of amino acids changes during gestation, but at both mid- and late gestation the total supply exceeds that required for growth. Weight-specific protein synthetic rate decreases with increasing gestational age, and these changes are proportional to the changes in metabolic rate. The use of multiple tracer methodology coupled with measurement of net tracer fluxes into and out of fetal and placental tissues can be used to delineate amino acid metabolism in considerable detail. Such studies demonstrate that even essential amino acids can be oxidized extensively by the fetus. The oxidation rate of leucine exceeds its rate of accretion in tissue proteins. Glycine metabolism is unique in several ways; there is a large umbilical uptake of glycine without a measurable uterine uptake. In late gestation there is no significant umbilical uptake of serine, although there is a significant uterine uptake, suggesting net uteroplacental utilization. Glycine is oxidized within the fetal liver and used for serum production. The interorgan exchange of amino acids between the fetal liver and placenta is clearly of major importance for serine and glycine metabolism and is likely to be of major importance for most nonessential amino acids.  相似文献   
210.
Provision of feed supplies for the rapidly growing livestock population is essential in West Asia. Shortages can be alleviated by growing forage crops in the existing fallow lands.
Narbon vetch (Vicia narbonensis L.) is a leguminous species with high yield potential, drought tolerance and cold resistance. It could be used for grain and straw production as source for animal feed in dry areas. It is good source of protein with seeds contain 28 % protein yielding 364 kg per hectare protein, whilst straw contains 9 % protein and yields a similar amounts of digestible protein per hectare.
Nine promising lines of Narbon vetch developed at ICARDA were tested at two sites over four years under rainfed conditions. Since climatic conditions were considered to be of considerable importance, each site in each year was treated as a separate environment to give eight environments with annual rainfall varied from 195 to 504 mm.
Narbon vetch possessed high seedling vigour with rapid winter growth and negligible cold damage. Grain yield varied form 0.47 to 1.90 t/ha, with a harvest index varying from 30 to 40 %. The data indicated that below 300 mm rainfall the grain yield varied from 0.47 t/ha when rain fall was 195 mm to 1.4 t/ha when rainfall was 245 mm. Most of the lines had wide adaptation to dry areas in terms of both grain yield and stability. Climate, except early spring rains had little effect on biological and grain yields.  相似文献   
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