排序方式： 共有47条查询结果，搜索用时 15 毫秒
41.
Koji Kageyama Ayako Nakashima Yuki Kajihara Haruhisa Suga Eric B. Nelson 《Journal of General Plant Pathology》2005,71(3):174-182
Isolates of Pythium graminicola and related species were differentiated using restriction fragment length polymorphism (RFLP) analyses of the internal transcribed spacer (ITS) regions of rDNA and the cytochrome c oxidase subunit II (COX II) gene. These sequences were used in subsequent phylogenetic analyses. Finally, the phylogenetic placement of species was compared to that determined from morphological characteristics. The 62 isolates tested were divided into seven groups, A–G, based on RFLP analysis of the rDNA-ITS region. In the RFLP analysis of the COX II gene, isolates were divided into groups similar to those based on ITS-RFLP. Groups A and B were each separated into two additional subgroups. Grouping of isolates based on RFLP analyses agreed with the morphological differentiation. Groups A, B, D, E, F, and G were identified as P. graminicola, P. arrhenomanes, P. aphanidermatum, P. myriotylum, P. torulosum, and P. vanterpoolii, respectively. Group C was closely related to group B based on phylogenetic analysis of the rDNA-ITS region and the COX II gene and is similar to P. arrhenomanes. Each of the other species occupied their own individual clades. Although P. arrhenomanes is morphologically similar to P. graminicola, our phylogenetic analyses revealed that it was evolutionarily distant from P. graminicola and more closely related to P. vanterpoolii. Our analysis also revealed that P. torulosum with smaller oogonia is more closely related to P. myriotylum with large oogonia than to P. vanterpoolii, which forms smaller oogonia and is morphologically similar to P. torulosum. P. aphanidermatum with large oogonia and aplerotic oospores was not related to the morphologically similar species P. myriotylum. Results suggest that P. graminicola and related species are phylogenetically distinct, and molecular analyses, in addition to morphological analyses, are necessary for the accurate taxonomic placement of species in this complex. 相似文献
42.
Haruhisa Suga Rina Okumura Maho Ikawa Masafumi Shimizu Koji Kageyama 《Plant pathology》2022,71(3):602-610
Members of the Fusarium graminearum species complex are known to cause head blight of wheat. A natural nonpathogenic mutant, strain Fa0233007, was found in the strain collection of the Japanese F. graminearum species complex. In addition, this strain cannot form perithecia. Complete linkage of these deficiencies in the progenies obtained from crossing a wild-type strain and the mutant strain suggested that a single genomic locus was responsible for deficiencies in both traits. The locus was mapped to chromosome 1 using sequence-tagged markers. An NADPH oxidase gene (NoxA, FGSG00739) was identified in the mapped region, and a 2-bp deletion that results in premature termination of the open reading frame was detected in the gene. Both pathogenicity and perithecium formation were recovered by FGSG00739 complementation in the mutant strain. 相似文献
43.
Sun Y Hayakawa S Ogawa M Fukada K Izumori K 《Journal of agricultural and food chemistry》2008,56(12):4789-4796
d-Psicose (Psi) might be an ideal sucrose (Suc) substitute for food products due to its sweet taste, easy processing, and functional properties (noncaloric and low glycemic response). In the present study, the effects of Psi on foaming properties of egg white (EW) protein and the quality of butter cookies were analyzed to find a better use of Psi in aerated food systems. The results showed that Psi could improve the foaming properties of EW protein with increasing whipping time in comparison to Suc and d-fructose (Fru). The addition of Psi to butter cookies, as partial replacement of Suc, had no influence on the cook loss while significantly contributing to a color change of the cookie crust through a nonenzymatic browning reaction. Furthermore, Psi-containing cookies possessed the highest antioxidant capacity in all tested cookies using two assays of radical scavenging activity and ferric reducing power. It was found that there was a close correlation between the crust color and the antioxidant activity of the cookie. The results suggest that the addition of Psi enhanced the browning reaction during cookie processing and, consequently, produced a strong antioxidant activity. 相似文献
44.
Fukada Haruhisa Kitagima Renato Shinagawa Junpei Morino Haruka Masumoto Toshiro 《Fisheries Science》2020,86(1):107-118
Docosahexaenoic acid (DHA) is an essential fatty acid for marine carnivorous fish. Algal meal (AM), available as a new dietary DHA source, could completely replace dietary fish oil (FO). In this study, dietary FO was replaced with plant oil mixtures and AM in juvenile yellowtail Seriola quinqueradiata to investigate its effects on growth performance and fatty acid composition. The FO control diet was prepared with only pollack liver oil as the lipid source. For the non-FO diets, pollack liver oil was completely replaced with mixtures of canola oil and palm oil, with AM supplementation at 0% (AM0), 1% (AM1), 2% (AM2), 3% (AM3), and 4% (AM4). After completion of the 8-week feeding trial, the AM2 group showed significantly higher values for final body weight and feed efficiency than the AM0 group. No significant differences were observed in the other parameters of growth performance. Whole-body fatty acid composition reflected the dietary fatty acid composition in all dietary groups. These findings demonstrate that AM is useful as a DHA source in yellowtail aquaculture, thus contributing to a reduction in the use of FO in fish diets.
相似文献45.
Effects of replacing fish oil with canola oil on the growth performance,fatty acid composition and metabolic enzyme activity of juvenile yellowtail Seriola quinqueradiata (Temminck & Schlegel, 1845) 
下载免费PDF全文
下载免费PDF全文 Haruhisa Fukada Etsunori Taniguchi Katsuji Morioka Toshiro Masumoto 《Aquaculture Research》2017,48(12):5928-5939
In this study, fish oil (FO) was replaced with canola oil (CO) in juvenile yellowtail diets to establish the optimal replacement levels that ensure adequate feed quality for aquaculture. Juvenile yellowtails (initial body weight: 104.7 g) were fed one of four diets for 10 weeks: 100% FO (FO group) or FO replacement with 25%, 50% or 100% CO (CO25, CO50 and CO100 groups respectively). Body weight, specific growth rate and feed efficiency were significantly affected by the replacement of FO with CO. The final body weight of the CO100 group was significantly lower than that of the FO (control) and CO50 groups. Furthermore, the CO50 group showed significantly higher feed efficiency than the FO group during the first 4 weeks (long photoperiod and high water temperature conditions). Increasing dietary CO increased serum glucose and triglyceride concentrations in a dose‐dependent manner. Regarding the proximate composition of fillet and liver, only crude protein in fillet was affected by the replacement of FO with CO. Fatty acid compositions of fillet and liver were correlated to the amount of CO in each diet. The activities of the metabolic enzymes phosphofructokinase and alanine aminotransferase were lowest in the CO25 and CO50 groups respectively. Thus, FO replacement with CO affected growth performance, serum components, fatty acid composition and the activity of metabolic enzymes. Overall, results obtained in the present study suggest that partial replacement (50%) of FO with CO is effective and might increase juvenile yellowtail growth under long photoperiod and/or high water temperature conditions. 相似文献
46.
Ayumi Seno-O Fumiaki Takakuwa Tomomi Hashiguchi Katsuji Morioka Toshiro Masumoto Haruhisa Fukada 《Fisheries Science》2008,74(6):1297-1306
Dietary fish oil (FO) was replaced by olive oil (OO) in young yellowtail Seriola quinqueradiata to investigate its effects on growth, muscular fatty acid composition and prevention of color deterioration of dark muscle
during storage. Yellowtail were fed one of four diets, where FO (80 g/kg diet) was replaced by OO (0, 25, 50 and 100%) for
40 days. No significant difference in growth was seen among the diet groups. In addition, these experimental diets did not
affect the proximate compositions of the dorsal muscle, ventral muscle and the liver in these fish. Serum total protein, glucose
and total cholesterol levels did not show significant differences; however, serum triglyceride levels were significantly higher
in fish fed 50 and 100% OO diets. Fatty acid composition of ventral muscle reflected the composition of the respective diets.
Dark muscle discoloration was reduced in fish fed OO diets after 12 to 18 h during storage at 4°C. Furthermore, the redness
value of fresh dark muscle increased depending on the extent of FO replacement. These results indicate that the partial or
total dietary replacement of FO with OO prevents discoloration of dark muscle without affecting the growth of young yellowtail
after 40 days of feeding. 相似文献
47.
Haruhisa Fukada Jon T. Dickey Andrew L. Pierce Nicholas Hodges Akihiko Hara Penny Swanson Walton W. Dickhoff 《Fish physiology and biochemistry》2003,28(1-4):335-336
The expression levels of growth hormone receptor (GHR) and insulin-like growth factor (IGF-I) in gonads of maturing coho salmon were measured by real-time PCR. The GHR and IGF-I mRNAs showed different gene expression pattern between male and female. These results suggest that a sexual dimorphism may exist in the GHR and IGF-I mRNA expressions of coho salmon during gonadal maturation 相似文献