Eye fatigue-reduction effect of wood rim cover on liquid crystal display     

Seiji Hirata  Isao Kageyama  Masamitsu Ohta 《Journal of Wood Science》2012,58(2):163-168

The purpose of this study is to confirm objectively by an instrumental measurement if there is less eye fatigue in VDT work when using a wood rim covered display than when using a plastic housing display. The subjects were 26 graduate and undergraduate university students. Each subject did the same 60-min VDT activity with both a plastic housing and wood-covered display. Before and after VDT activity, each subject’s near-point accommodation distance was measured by a near-point ruler. As a result, it was confirmed that variation rate of the near-point accommodation distance by the VDT work with the wood-covered display was smaller than with the plastic housing display. From sensory tests, progression of subjective symptoms such as eye pain and headache was less with the wood-covered display than with the plastic housing display.  相似文献   

Intestinal Tritrichomonas suis (=T. foetus) infection in Japanese cats     

Doi J  Hirota J  Morita A  Fukushima K  Kamijyo H  Ohta H  Yamasaki M  Takahashi T  Katakura K  Oku Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(4):413-417

Tritrichomonas suis (=T. foetus) has recently been reported to be a causative agent of chronic large-bowel diarrhea in cats. While the disease was previously attributed to Pentatrichomonas hominis, the etiologic agent for feline trichomonal diarrhea was identified as T. suis. Although feline trichomonosis due to T. suis has been reported at prevalences ranging from 14 to 31% in Europe and the U.S., no reports of the pathogen have been published to date in Japan. In 2008, however, we encountered a case of feline trichomonosis at the Veterinary Teaching Hospital of Hokkaido University. The parasite was identified as T. suis by nested PCR amplification of partial internal transcribed spacer region 1 and 5.8S ribosomal RNA gene sequences with T. suis-specific primers and DNA sequencing of the amplified products. We then conducted surveys for feline trichomonosis in three different animal hospitals using either cultivation and/or PCR-based assays. The results revealed that 13 of 147 samples (8.8%) were positive for T. suis, and that 5 of the 13 infected cats, which ranged between 1 month and 7.5 years-old, showed chronic diarrhea. Seven of the infected cats were purebred and 6 were mixed breed. These findings suggested that feline trichomonosis is prevalent in Japan, and that T. suis may play a role as a causative agent of feline chronic diarrhea.  相似文献   

Optimization of a protocol for cryopreservation of mouse spermatozoa using cryotubes     

Hasegawa A  Yonezawa K  Ohta A  Mochida K  Ogura A 《The Journal of reproduction and development》2012,58(1):156-161

The rapid increase in the number of genetically modified mouse strains has produced a high demand for their frozen spermatozoa from laboratories and mouse banking facilities. Historically, plastic straws have been used preferentially as containers for frozen mammalian spermatozoa because spermatozoa frozen in plastic straws have a high survival rate after thawing. However, plastic straws are more fragile and are used less often than the cryotubes used for conventional cell freezing. In this study, we sought to develop a new protocol for sperm freezing using cryotubes as the container to increase the accessibility of mouse sperm cryopreservation. Epididymal spermatozoa were collected from mature ICR or C57BL/6J (B6) males and were suspended in 18% raffinose and 3% skim milk solution. We then optimized the following conditions using the sperm survival rate as an index: 1) distance of cryotubes from the surface of the liquid nitrogen at freezing, 2) volume of the sperm suspension in the cryotube and 3) temperature of warming sperm during thawing. The best result was obtained when cryotubes containing 10 μl of sperm suspension were immersed 1 cm below the surface of the liquid nitrogen and then thawed at 50 C. The fertilization rates using spermatozoa frozen and thawed using this method were 63.1% in ICR mice and 28.2% in B6 mice. The latter rate was increased to 62.3% by adding reduced glutathione to the fertilization medium. After embryo transfer, 68% and 62% of the fertilized oocytes developed into normal offspring in the ICR and B6 strains, respectively. These results show that cryotubes can be used for cryopreservation of mouse spermatozoa under optimized conditions. This protocol is easy and reproducible, and it may be used in laboratories that do not specialize in sperm cryopreservation.  相似文献   

Effects of prescription diet on dealing with stressful situations and performance of anxiety-related behaviors in privately owned anxious dogs     

Maki Kato  Kazuki Miyaji  Nobuyo Ohtani  Mitsuaki Ohta 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(1):21-26

The objectives of this study were to evaluate the effects of a diet (CALM CANINE) supplemented with alpha-casozepine and L-tryptophan on the stress response of anxious dogs with a focus on anxiety-related behavioral parameters and physiological parameters. Forty-four privately owned dogs were first fed with the control diet, followed by the study diet, each for an 8-week period, with a transitional period of 1 week between the diets. After 7 weeks on a particular diet, owners reported their dogs' behavior by filling out a questionnaire. Using the Canine Behavioral Assessment and Research Questionnaire, the effect of the study diet was found to be significant for 4 anxiety-related behavioral parameters, possibly mirroring a placebo effect. After 7 weeks on each diet, the urine cortisol-to-creatinine ratio (UCCR) was measured to assess the stress response to a visit to a veterinary practice for toenail clipping. UCCR was measured to complement the interpretation of behavioral data with regard to the stress susceptibility of the dogs. A positive correlation was found between baseline UCCR, as measured in the home situations, and poststressor UCCR. The stressor-induced increase was significantly lower in the dogs when they were fed the study diet than earlier when they were fed the control diet. The supplementation of casozepine or the proportion of protein in a given diet could have been a determining factor. The study diet seems to improve the ability of an individual to cope with stress and may reduce anxiety-related behavior in anxious dogs.  相似文献   

Effects of chronic obesity and weight loss on plasma ghrelin and leptin concentrations in dogs     

Jeusette IC  Detilleux J  Shibata H  Saito M  Honjoh T  Delobel A  Istasse L  Diez M 《Research in veterinary science》2005,79(2):169-175

The objective of this study was to evaluate, in dogs, the effects of obesity and weight loss on plasma total ghrelin and leptin concentrations. Twenty-four Beagle dogs, 12 control lean and 12 obese dogs of both genders and aged between 1 and 9 years, were used for the experiments. Mean body weight was 12.7+/-0.7 kg for the lean group and 21.9+/-0.8 kg for the obese group. The trial was divided into three phases. During phase 1, all 24 Beagle dogs were fed a maintenance diet. During phase 2, the obese dogs were submitted to a weight loss protocol with a high protein-low energy diet. The weight loss protocol ended once dogs reached optimal body weight. During phase 3, the dogs that were submitted to the weight loss protocol were maintained at their optimal body weight for 6 months. Plasma total ghrelin, leptin, insulin and glucose concentrations were measured to evaluate the effects of obesity and weight loss on these parameters in dogs. Body weight, body condition score, thoracic and pelvic perimeters, and ingested food amounts were also recorded during the study. Obese dogs demonstrated a significant decrease in plasma ghrelin and a significant increase in plasma leptin and insulin concentrations when compared with control dogs. During weight loss, significant increases in plasma total ghrelin and glucose and significant decreases in plasma leptin and insulin were observed. The increase in plasma ghrelin concentrations seemed to be transient. Body weight and the morphometric parameters correlated positively with leptin concentrations and negatively with total ghrelin concentrations. These results suggest that ghrelin and leptin could play a role in dogs in the adaptation to a positive or negative energy balance, as observed in humans.  相似文献   

Yellowing disease of tomatoes caused by <Emphasis Type="Italic">Tomato infectious chlorosis virus</Emphasis> newly recognized in Japan     

Sedyo Hartono  Tomohide Natsuaki  Haruki Sayama  Hiroki Atarashi  Seiichi Okuda 《Journal of General Plant Pathology》2003,69(1):61-64

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Plasma glucose,mannose, and non-esterified fatty acid concentrations in layer-type chickens     

T. Sato  S. Soeda  Y. Ohta  K. Katayama  T. Arai  H. Tazaki 《British poultry science》2008,49(6):731-735

1. The oral administration of glucose or dietary glucose reduces fasting plasma mannose concentrations in mammals. On the other hand, there have been no reports on plasma mannose levels in birds. We have analysed chicken plasma mannose and glucose by an original high-performance liquid chromatography (HPLC) method, together with plasma non-esterified fatty acid (NEFA) concentrations in chickens.

2. Plasma glucose concentrations of chickens did not differ among three different age groups (0, 18 and 150 d). However, the plasma mannose concentrations of chicks at the age of 0 d were higher than those of chickens at the ages of 18 and 150 d.

3. At the age of 18 and 150 d, plasma glucose concentrations were elevated and plasma mannose and NEFA concentrations were decreased after regular feeding, compared to fasting levels.  相似文献   

Variability of fracture toughness by the crack tip position in an annual ring of coniferous wood     

Kosei Ando  Masamitsu Ohta 《Journal of Wood Science》1999,45(4):275-283

The effects of the location of the crack tip in an annual ring and the direction of crack propagation on the fracture toughness of the TR crack propagation system of coniferous wood (T, direction normal to the notch plane; R, propagation direction) were analyzed by the finite element method in regard of the changes in elastic modulus and strength within an annual ring. The critical point of the fracture was defined as the state where the stress of a square element (0.125 × 0.125 mm) in contact with the crack tip equals the tensile strength. The distribution of specific gravity was measured by soft X-ray densitometry. The elastic moduli in the T and R directions were estimated by the sound velocity. The tensile strengths in the T and R directions were measured by the tensile test using small specimens of l mm span length. Regarding the variability of fracture toughness (K _IC), the experimental and calculated results had the same tendency. Therefore, it was concluded that the variability ofK _IC is caused by the (1) heterogeneity of the elastic modulus and strength within an annual ring; and (2) changes in the degree of stress concentration at the crack tips, according to the direction of crack propagation.Part of this work was presented at the 40th annual meeting of the Japan Wood Research Society, Tsukuba, April 1990 and at the 6th International Conference on Mechanical Behavior of Materials, Kyoto, July 1991  相似文献   

Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel,Anguilla japonica,at Completion of Yolk Resorption     

Tadahide Kurokawa  Takuma Okamoto  Koichiro Gen  Susumu Uji  Koji Murashita  Tatsuya Unuma  Kazuharu Nomura  Hajime Matsubara  Shin‐Kwon Kim  Hiromi Ohta  Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735

The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity.  相似文献   

The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture     

H. Tanaka  H. Kagawa  H. Ohta  T. Unuma  K. Nomura 《Fish physiology and biochemistry》2003,28(1-4):493-497

Weekly injections of salmon pituitary extracts (SPE) were administered to female Japanese eel, Anguilla japonica at a dose of 20 mg/fish. This induced vitellogenesis and caused oocytes to reach the migratory nucleus stage. Later, a majority of the females that received an injection of SPE at a priming dose, followed 24 h later by 17,20β-dihydroxy-4-pregnen-3-one (DHP), ovulated 15 to 18 h after the final injection. In cultivated males, repeated injections of human chorionic gonadotropin (hCG) at a dose of 1 IU/g BW/week induced spermatogenesis and spermiation. Since potassium ions were revealed to be an essential constituent for the maintenance of motility in the eel spermatozoa, artificial seminal plasma containing KCl was designed as a diluent of milt, and enabled the preservation of milt for several weeks in refrigeration. As a result, artificial fertilization performed immediately after ovulation with pre-diluted and stocked milt consistently resulted in the production of high-quality gametes. Recently, a slurry-type diet made from shark egg yolk has been found to be a suitable feed for captive-bred eel larvae. Although preleptocephalus larvae can be reared with this diet beyond the depletion of their yolk and oil droplet stores, it remains inadequate because larvae reared under this way cannot be raised to the following stage. Therefore, the diet was improved by supplements of krill hydrolysate, soybean peptide, vitamins and minerals. Larvae fed on this new diet have grown to 50 to 60 mm in total length (TL), and have begun to metamorphose into glass eels approximately 250 days after hatching.  相似文献