The Population Structure of Phytophthora infestans from the Toluca Valley of Central Mexico Suggests Genetic Differentiation Between Populations from Cultivated Potato and Wild Solanum spp     

Flier WG  Grünwald NJ  Kroon LP  Sturbaum AK  van den Bosch TB  Garay-Serrano E  Lozoya-Saldaña H  Fry WE  Turkensteen LJ 《Phytopathology》2003,93(4):382-390

ABSTRACT The population structure of Phytophthora infestans in the Toluca Valley of central Mexico was assessed using 170 isolates collected from cultivated potatoes and the native wild Solanum spp., S. demissum and S. xendinense. All isolates were analyzed for mitochondrial DNA (mtDNA) haplotype and amplified fragment length polymorphism (AFLP) multi-locus fingerprint genotype. Isolate samples were monomorphic for mtDNA haplotype because all isolates tested were of the Ia haplotype. A total of 158 multilocus AFLP genotypes were identified among the 170 P. infestans isolates included in this study. P. infestans populations sampled in the Toluca Valley in 1997 were highly variable and almost every single isolate represented a unique genotype based on the analysis of 165 AFLP marker loci. Populations of P. infestans collected from the commercial potato-growing region in the valley, the subsistence potato production area along the slopes of the Nevado de Toluca, and the native Solanum spp. on the forested slopes of the volcano showed a high degree of genetic diversity. The number of polymorphic loci varied from 20.0 to 62.4% for isolates collected from the field station and wild Solanum spp. On average, 81.8% (135) of the AFLP loci were polymorphic. Hetero-zygosity varied between 7.7 and 19.4%. Significant differentiation was found at the population level between strains originating from cultivated potatoes and wild Solanum spp. (P = 0.001 to 0.022). Private alleles were observed in individual isolates collected from all three populations, with numbers of unique dominant alleles varying from 9 to 16 for isolates collected from commercial potato crops and native Solanum spp., respectively. Four AFLP markers were exclusively found present in isolates collected from S. demissum. Indirect estimation of gene flow between populations indicated restricted gene flow between both P. infestans populations from cultivated potatoes and wild Solanum hosts. There was no evidence found for the presence of substructuring at the subpopulation (field) level. We hypothesize that population differentiation and genetic isolation of P. infestans in the Toluca Valley is driven by host-specific factors (i.e., R-genes) widely distributed in wild Solanum spp. and random genetic drift.  相似文献   

A genomics approach to crop pest and disease research     

Keon J  Curtis R  Cabrera H  Hargreaves J 《Pest management science》2003,59(2):143-148

Genome-wide analyses of gene function and gene expression are beginning to yield valuable information in many areas of biological research, and these genomic tools are now being applied to crop pest and disease research. DNA sequencing of cDNA libraries to generate sets of expressed sequence tags (ESTs) are allowing gene compendiums for crop diseases to be compiled. Annotation of such data collections is also providing a wealth of functional information about gene products through similarities to proteins with known function. The next phase of the functional genomics era will be to employ large-scale techniques to knock out or silence genes in order to synthesize gene-specific mutants for phenotypic analysis and to use micro-array methodology to analyze global gene expression, protein turnover and protein processing during the processes of parasitism and colonization. Application of these technologies promises to accelerate the pace that biological information relevant to crop protection accrues. The ability of researchers to assimilate this information into complex models and workable hypotheses is, thus, set to revolutionize the way we study pests and diseases of crop plants.  相似文献   

Transfer of irrigation scheduling technology in Mexico     

Pedroza Hector Quiones  Helene Unland  Waldo Ojeda  Ernesto Sifuentes 《Agricultural Water Management》1999,40(2-3):333-339

In Mexico most of the agricultural production originates from large irrigation districts in the northern part of the country. This region is characterized by its semiarid desert climate with a winter rainy season dominated by frontal storms, and a summer monsoon season dominated by highly localized convective storms, yielding most of the annual precipitation. Essentially all irrigation needs must be met by surface water stored in various reservoirs. Precipitation is, therefore, the most important limiting factor in Mexico's agricultural production. Traditionally, long-time averages of statistical climate data from few and widely-spaced weather stations were used to determine frequency and amount of water applied, and the algorithms employed usually did not consider the effects of great spatial climate variability and plant physiology. In the past five years, great parts of Mexico, especially in the North, have been affected by severe water shortages resulting from insufficient precipitation (perhaps related to the ‘El Niño' phenomenon), combined with inefficient water resources management. Irrigation districts increasingly have to deal with the considerable uncertainty in water resources availability as a limiting factor in the decision making process. In order to address these irrigation water shortages, the Mexican National Water Commission and the Mexican Water Resources Institute are introducing new technologies using agrometeorological networks for more efficient, real-time irrigation scheduling in the main irrigation districts of Mexico. Validation plots established in one particular irrigation district (Carrizo Valley, Sinaloa), demonstrate water savings in the order of at least 20% without any appreciable decrease in crop yields.  相似文献   

Modeling Elastic and Geometric Properties of Coffea arabica L. var. Colombia Fruits by an Experimental-numerical Approach     

Hector A. Tinoco 《International Journal of Fruit Science》2017,17(2):159-174

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Distribution and reproduction of Procambarus clarkii in relation to water management,salinity and habitat type in the Camargue          下载免费PDF全文

Eric Meineri  Hector Rodriguez‐Perez  Samuel Hilaire  François Mesleard 《水产资源保护:海洋与淡水生态系统》2014,24(3):312-323

Factors influencing half‐pearl (mabe) production and quality in the red abalone Haliotis rufescens     

Alejandro Rojas‐Figueroa  Rubn Araya  Hector Acosta‐Salmn  Felipe Chvez‐Contreras  Nadia L. Ortiz‐Cornejo  Pedro E. Saucedo 《Aquaculture Research》2019,50(11):3392-3400

The factors influencing half‐pearl (mabe) production in the red abalone Haliotis rufescens were investigated, as a strategy to optimize the technology for the development of a pearling industry in Chile. The effects of abalone size (small, large), implant position (dorsal, central, ventral and their combinations), and number of nuclei (one, two, three) were analysed on the quantity, thickness of the nacre layer and quality (shape, colour, lustre, defects) of the mabe produced. The size of abalone did not exert a clear influence on the quantity and quality of mabe, but the position on the shell (firstly) and number of nuclei (secondly) did. More mabe, with thicker nacre, brighter lustre and fewer defects occurred in small or large abalone implanted with only one nucleus on dorsal position. In contrast, small and large abalone implanted in the ventral position formed less mabe, with thinner nacre, poor lustre and more defects. This likely occurs because mantle tissue folds at the ventral position and does not always cover the nucleus, leaving it partially or totally exposed. Most of the pearls were categories AA in small abalone and A in large abalone; only three AAA gems were produced in large abalone with one nucleus on dorsal position or with three nuclei (DCV). Based on these results, we recommend implanting abalone at 60–100 mm shell length, with only one nucleus on the dorsal position.  相似文献   

Pharmacokinetics of a peroral single dose of two long-acting formulations and an aqueous formulation of doxycycline hyclate in horses     

Heidi Zozaya  Lilia Gutierrez  Maria Josefa Bernad  Hector Sumano 《Acta veterinaria Scandinavica》2013,55(1):21

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Preovulatory serum estradiol concentration is positively associated with oocyte ATP and follicular fluid metabolite abundance in lactating beef cattle     

Casey C Read  J Lannett Edwards  F Neal Schrick  Justin D Rhinehart  Rebecca R Payton  Shawn R Campagna  Hector F Castro  Jessica L Klabnik  Sarah E Moorey 《Journal of animal science》2022,100(7)

Cattle induced to ovulate a small, physiologically immature preovulatory follicle had reduced oocyte developmental competence that resulted in decreased embryo cleavage and day 7 embryo quality compared with animals induced to ovulate a more advanced follicle. RNA-sequencing was performed on oocytes and their corresponding cumulus cells approximately 23 h after gonadotropin-releasing hormone (GnRH) administration to induce the preovulatory gonadotropin surge suggested reduced capacity for glucose metabolism and oxidative phosphorylation in the cumulus cells and oocytes from follicles ≤11.7 mm, respectively. We hypothesized that induced ovulation of a small, physiologically immature preovulatory follicle results in a suboptimal follicular microenvironment and reduced oocyte metabolic capacity. We performed a study with the objective to determine the impact of preovulatory follicle diameter and serum estradiol concentration at GnRH administration on oocyte metabolic competence and follicular fluid metabolome profiles. We synchronized the development of a preovulatory follicle and collected the follicle contents via transvaginal aspiration approximately 19 h after GnRH administration in lactating beef cows (n = 319). We determined ATP levels and mitochondrial DNA (mtDNA) copy number in 110 oocytes and performed ultra-high-performance liquid chromatography–high resolution mass spectrometry metabolomic studies on 45 follicular fluid samples. Intraoocyte ATP and the amount of ATP produced per mtDNA copy number were associated with serum estradiol concentration at GnRH and time from GnRH administration to follicle aspiration (P < 0.05). mtDNA copy number was not related to follicle diameter at GnRH, serum estradiol concentration at GnRH, or any potential covariates (P > 0.10). We detected 90 metabolites in the aspirated follicular fluid. We identified 22 metabolites associated with serum estradiol concentration at GnRH and 63 metabolites associated with follicular fluid progesterone concentration at the time of follicle aspiration (FDR < 0.10). Pathway enrichment analysis of significant metabolites suggested altered proteinogenesis, citric acid cycle, and pyrimidine metabolism in follicles of reduced estrogenic capacity pre-gonadotropin surge or reduced progesterone production by the time of follicle aspiration.  相似文献   

Fisheries and conservation assessment of sharks in Pacific Panama     

Hector M. Guzman  Roberto Cipriani  Angel J. Vega  Jorge Manuel Morales‐Saldaa 《水产资源保护:海洋与淡水生态系统》2020,30(2):315-330

1. In Panama, shark fisheries were initially developed in the 1980s and progressively increased in production in the 1990s mainly due to the high demand for shark fins and meat from the international Asian market. Since then, and despite the exploitation rate (average 3,514 t year^–1) and endangered status of some species, shark fisheries have seldom been studied, and official statistics are general or incomplete and not suitable for the development of appropriate conservation and management strategies.
2. To understand the dynamics of shark fisheries in Panama, field surveys were conducted between 2007 and 2009 at several landing ports of small‐scale and industrial fisheries, at fish processing plants and on‐board fishing vessels in Pacific Panama, where most of the fishing vessels of the country operate.
3. In general, it was found that the artisanal and industrial fisheries of the Pacific coast of Panama regularly exploit at least 18 species of sharks, which are also being exploited by neighbouring countries in the eastern Pacific, suggesting the importance of coordinated conservation initiatives across the multiple jurisdictions. A large number of the individuals caught were immature, implying a certain level of impact on recruitment rates. This pattern was particularly evident in species such as Sphyrna lewini, for which immature individuals represented at least 99% and 63% of the total catch by small‐scale and industrial fisheries, respectively. Catch per unit of effort analyses showed that Carcharhinus and Sphyrna species were the most exploited (representing ~80% of the catches) by industrial fisheries in Panama between 2006 and 2009, suggesting that fishery management should provide special attention to these groups.
4. It is expected that the information presented here provides a baseline to develop new regulations, including the implementation of annual quotas and fishing seasons and the protection of nursery areas, for the long‐term sustainability and conservation of sharks in Panama.

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Hematologic and serum biochemical reference values for the wild Spectacled Caiman, Caiman crocodilus crocodilus, from the Venezuelan plains     

Rossini M  García G  Rojas J  Zerpa H 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(3):374-379

Background: Commercial farming of Caiman crocodilus crocodilus has had an impact on the use of this species for meat consumption and the leather industry. Spectacled Caimans comprise part of the South American plains biodiversity. Misinterpretation of laboratory data is a risk owing to the limited hematologic and serum biochemical values available for this species. Objective: The aim of this study was to determine hematologic and serum biochemical values for wild Spectacled Caimans from the Venezuelan plains. Methods: Blood samples were collected form the caudal tail vein of 100 Spectacled Caimans (40 males and 60 females) from the plains located in the State of Apure. Values for RBC count, PCV, hemoglobin concentration, WBC absolute and differential counts, and thrombocyte counts were obtained using manual methods, and RBC indices were calculated. Serum biochemical analysis included measurement of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and creatine kinase activities and concentrations of total protein and albumin. Comparisons between sexes were analyzed using the Mann–Whitney test. Results: Reference values for wild Spectacled Caimans were determined. Minor differences in hematologic values, particularly for RBC counts, were found compared with previously published values for this species. Serum biochemical values were similar to those available for other crocodilians. There were no significant differences between males and females. Conclusions: Minor differences between the values obtained for wild Spectacled Caimans and those previously published for this species may be related to differences in methodology and environmental conditions. Availability of hematologic and serum biochemical reference values will be useful for accurate diagnosis and management of disease in this species.  相似文献