首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   539篇
  免费   28篇
林业   15篇
农学   18篇
基础科学   3篇
  101篇
综合类   67篇
农作物   59篇
水产渔业   52篇
畜牧兽医   205篇
园艺   12篇
植物保护   35篇
  2023年   8篇
  2022年   10篇
  2021年   17篇
  2020年   19篇
  2019年   15篇
  2018年   15篇
  2017年   10篇
  2016年   10篇
  2015年   14篇
  2014年   14篇
  2013年   31篇
  2012年   32篇
  2011年   45篇
  2010年   23篇
  2009年   20篇
  2008年   23篇
  2007年   32篇
  2006年   34篇
  2005年   22篇
  2004年   34篇
  2003年   22篇
  2002年   21篇
  2001年   10篇
  2000年   3篇
  1998年   4篇
  1996年   5篇
  1994年   3篇
  1993年   4篇
  1992年   4篇
  1989年   5篇
  1988年   5篇
  1987年   2篇
  1984年   2篇
  1983年   6篇
  1980年   2篇
  1979年   2篇
  1978年   4篇
  1977年   3篇
  1976年   2篇
  1975年   2篇
  1973年   2篇
  1972年   2篇
  1971年   2篇
  1966年   3篇
  1959年   1篇
  1956年   1篇
  1954年   1篇
  1938年   1篇
  1937年   1篇
  1936年   1篇
排序方式: 共有567条查询结果,搜索用时 15 毫秒
61.
Seagrasses, which form critical subtidal habitats for marine organisms worldwide, are fragmented via natural processes but are increasingly being fragmented and degraded by boating, fishing, and coastal development. We constructed an individual-based model to test how habitat fragmentation and loss influenced predator–prey interactions and cohort size for a group of settling juvenile blue crabs (Callinectes sapidus Rathbun) in seagrass landscapes. Using results from field studies suggesting that strong top-down processes influence the relationship between cannibalistic blue crab populations and seagrass landscape structure, we constructed a model in which prey (juvenile blue crabs) are eaten by mesopredators (larger blue crabs) which in turn are eaten by top-level predators (e.g., large fishes). In our model, we varied the following parameters within four increasingly fragmented seagrass landscapes to test for their relative effects on cohort size: juvenile blue crab (prey) predator avoidance response, hunting ability of mesopredators and predators, the presence of a top-level predator, and prey settlement routines. Generally, prey cohort size was maximized in the presence of top-level predators and when mesopredators and predators exhibited random searching behavior vs. directed hunting. Cohort size for stationary (tethered) prey was maximized in fragmented landscapes, which corresponds to results from field experiments, whereas mobile prey able to detect and avoid predators had higher survival in continuous landscapes. Prey settlement patterns had relatively small influences on cohort size. We conclude that the effects of seagrass fragmentation and loss on organisms such as blue crabs will depend heavily on behaviors of prey and predatory organisms and how these behaviors change with landscape structure.  相似文献   
62.
Multispecies swards, comprised of different plant functional groups have comparable production potential to high N input L. perenne swards at reduced N when legumes are included. However, information on the appropriate management of multispecies swards is limited. The effect of differing management practices on the herbage dry-matter (DM) production and botanical composition of different sward types was investigated using a 3 × 2 × 2 factorial design plot experiment. The experiment consisted of three sward types: L. perenne-only receiving (LP; 250 kg N ha−1 a−1); L. perenne-Trifolium repens (LP-TR); multispecies sward containing L. perenne, Phleum pratense, Trifolium repens, Trifolium pratense, Plantago lanceolata and Cichorium intybus (MSS). LP-TR and MSS received 90 kg N ha−1 a−1. Harvesting of plot herbage took place every 21 or 28 days to a defoliation height of 4 or 6 cm, over two growing seasons (March to November 2020 and 2021 inclusive). Annual herbage produced by both LP and LP-TR was not significantly affected by defoliation frequency. However, MSS produced 1923 kg DM ha−1 a−1 more herbage when harvested less frequently. Unsown species contributed significantly less to the herbage DM of MSS compared to LP and LP-TR and remained similar in 2020 and 2021, whereas the contribution of unsown species to herbage DM increased in LP and LP-TR from 2020 to 2021, with noxious species more associated with LP and LP-TR than MSS. Results demonstrate the role of multispecies swards in improving the sustainability of grass-based agricultural systems in an environment of reduced fertilizer inputs.  相似文献   
63.
64.
65.
The starch granule of maize contains a characteristic set of tightly bound polypeptides. Granule-associated polypeptides are typically extracted from starch granules by heating starch granule suspensions at 90–100°C in a detergent such as SDS. Solubilized proteins are recovered by centrifugation and analyzed by gel electrophoresis. Previously identified tightly bound granule intrinsic proteins consist of the 85-kDa starch-branching enzyme IIb, the 76-kDa starch synthase I, and the 60-kD waxy (Wx) protein, also known as granule-bound starch synthase I. However, SDS extracts from starch granules of maize also contain a cluster of proteins ranging in mass between 47 and 32 kDa In this study, we analyzed this group of granule-associated proteins and found that each was recognized by the Wx antibody. A 15 amino acid N-terminal sequence from the 47-kDa polypeptide was identical to the predicted N-terminus of the Wx protein. Further analysis revealed that each immunoreactive polypeptide between 47 and 32 kDa was a heat-induced fragmentation product of the Wx protein. Conditions for the extraction of granule proteins were evaluated. Our results demonstrate that granule proteins are effectively released by mild extraction (10-min incubation at 72°C). Relative to the Wx protein, starch synthase I and starch branching enzyme IIb were less susceptible to thermal fragmentation. These results demonstrate that the 85-, 76-, and 60-kDa polypeptides are authentic granule-intrinsic proteins, and that the majority of polypeptides between 47 and 32 kDa are artifacts of high-temperature granule extraction procedures.  相似文献   
66.
A six‐week feeding trial was conducted to determine the effects of different concentrations of fucoidan (1 g/kg, 10 g/kg and 30 g/kg; w/w) from Undaria pinnatifida on gibel carp (Carassius auratus gibelio). Our results demonstrated that 30 g/kg fucoidan significantly increased (p < .05) growth performance, intestinal digestive enzyme activities, acid phosphatase activity and immunoglobulin M content. Histological examinations revealed that gibel carp receiving 30 g/kg fucoidan had significant higher abundance of mucin‐containing goblet cells in middle and distal intestine as compared with control treatment (p < .05). Intestinal microbiota analysis showed that 30 g/kg fucoidan supplementation significantly increased (p < .05) the abundance of Cetobacterium and Aeromonas, but lowered (p < .05) the prevalence of pathogenic bacteria Plesiomonas and a mucin‐degrading bacterium Mucinivorans. Furthermore, RNA‐seq and RT‐qPCR analysis indicated that 30 g/kg fucoidan caused significant changes (p < .05) in the expression of genes involved in immune regulation (such as interleukin‐8 and cyclooxygenase), signal transduction (such as phosphatidylinositol‐4,5‐bisphosphate 3‐kinase and protein kinase B) and nutrition utilization (maltase–glucoamylase and muscarinic acetylcholine receptor 3). Together, the current study shows that fucoidan supplementation could elevate the activity of intestinal digestive enzymes, modulate intestinal microbial communities and potentiate a higher state of immune readiness, which might consequently improve growth performance and intestine health status of gibel carp.  相似文献   
67.
Salmonid alphavirus (SAV) causes pancreas disease (PD) in farmed Atlantic salmon (Salmo salar L.), and exocrine pancreas tissue is a primary target of the virus. Digestive enzymes secreted by the exocrine pancreas break down macromolecules in feed into smaller molecules that can be absorbed. The effect of SAV infection on digestion has been poorly studied. In this study, longitudinal observations of PD outbreaks caused by SAV subtype 2 (SAV2) in Atlantic salmon at two commercial sea sites were performed. The development of PD was assessed by measurement of SAV2 RNA load and evaluation of histopathological lesions typical of PD. Reduced digestion of both protein and fat co‐varied with the severity of PD lesions and viral load. Also, the study found that during a PD outbreak, the pen population comprise several subpopulations, with different likelihoods of being sampled. The body length of sampled fish deviated from the expected increase or steady state over time, and the infection status in sampled fish deviated from the expected course of infection in the population. Both conditions indicate that disease status of the individual fish influenced the likelihood of being sampled, which may cause sampling bias in population studies.  相似文献   
68.

Purpose

Better understanding of N transformations and the regulation of N2O-related N transformation processes in pasture soil contributes significantly to N fertilizer management and development of targeted mitigation strategies.

Materials and methods

15N tracer technique combined with acetylene (C2H2) method was used to measure gross N transformation rates and to distinguish pathways of N2O production in two Australian pasture soils. The soils were collected from Glenormiston (GN) and Terang (TR), Victoria, Australia, and incubated at a soil moisture content of 60% water-filled pore space (WFPS) and at temperature of 20 °C.

Results and discussion

Two tested pasture soils were characterized by high mineralization and immobilization turnover. The average gross N nitrification rate (ntot) was 7.28 mg N kg?1 day?1 in TR soil () and 5.79 mg N kg?1 day?1 in GN soil. Heterotrophic nitrification rates (nh), which accounting for 50.8 and 41.9% of ntot, and 23.4 and 30.1% of N2O emissions in GN and TR soils, respectively, played a role similar with autotrophic nitrification in total nitrification and N2O emission. Denitrification rates in two pasture soils were as low as 0.003–0.004 mg N kg?1 day?1 under selected conditions but contributed more than 30% of N2O emissions.

Conclusions

Results demonstrated that two tested pasture soils were characterized by fast N transformation rates of mineralization, immobilization, and nitrification. Heterotrophic nitrification could be an important NO3?–N production transformation process in studied pasture soils. Except for autotrophic nitrification, roles of heterotrophic nitrification and denitrification in N2O emission in two pasture soils should be considered when developing mitigation strategies.
  相似文献   
69.
Precision agriculture (PA) technologies have great potential for promoting sustainable intensification of food production, ensuring targeted delivery of agricultural inputs, and hence food security and environmental protection. The benefits of PA technologies are applicable across a broad range of agronomic, environmental and rural socio-economic contexts globally. However, farmer and land-manager adoption in low to middle income countries has typically been slower than that observed in more affluent countries. China is currently engaged in the process of agricultural modernisation to ensure food security for its 1.4 billion population and has developed a portfolio of policies designed to improve food security, while simultaneously promoting environmental protection. Particular attention has been paid to the reduction of agricultural inputs such as fertilisers and pesticides. The widespread adoption of PA technologies across the Chinese agricultural landscape is central to the success of these policies. However, socio-economic and cultural barriers, farm scale, (in particular the prevalence of smaller family farms) and demographic changes in the rural population, (for example, the movement of younger people to the cities) represent barriers to PA adoption across China. A framework for ensuring an acceptable and accelerated PA technology trajectory is proposed which combines systematic understanding of farmer and end-user priorities and preferences for technology design throughout the technology development process, and subsequent end-user requirements for implementation (including demonstration of economic and agronomic benefits, and knowledge transfer). Future research will validate the framework against qualitative and quantitative socio-economic, cultural and agronomic indicators of successful, or otherwise, PA implementation. The results will provide the evidence upon which to develop further policies regarding how to secure sustainable food production and how best to implement PA in China, as well as practical recommendations for optimising end-user uptake.  相似文献   
70.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号