Global proteomics analysis of testis and ovary in adult zebrafish (Danio rerio)     

Groh KJ  Nesatyy VJ  Segner H  Eggen RI  Suter MJ 《Fish physiology and biochemistry》2011,37(3):619-647

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Present status, and social and economic significance of inland fisheries in Germany   总被引：1，自引：0，他引：1  

H. Wedekind  V. Hilge  & W. Steffens 《Fisheries Management and Ecology》2001,8(4-5):405-414

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Cartilage thickness of the trochlea of the talus, with emphasis on sites predisposed to osteochondrosis dissecans, in clinically normal juvenile and adult dogs     

Brunnberg MM  Engelke E  Gielen IM  van Bree HJ  Hoffmann JE  Brunnberg L  Waibl HR 《American journal of veterinary research》2011,72(10):1318-1324

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Sperm migration in pigs after deep intrauterine and intraperitoneal insemination     

Brüssow KP  Torner H  Rátky J 《The Journal of reproduction and development》2011,57(3):342-345

Deep intrauterine insemination in pigs allows sperm deposition only into one uterine horn, but bilateral fertilization of oocytes occurs. How the sperm reach the contralateral oviduct remains disputable. The aim of this experiment was to study possible transperitoneal and/or transuterine sperm migration ways. Follicle growth and ovulation were induced in 24 peripubertal gilts with eCG and hCG 72 h after eCG. Endoscopic intrauterine insemination (IUI) was performed 32 h after hCG with 20 ml of extended semen (60 × 10(6) spermatozoa) as follows: Group CONTROL (n=8) received IUI into the right horn, and the left horn served as non-treated control; Group LIGATURE (n=8) received IUI into the right horn, and the left horn was closed by endoscopic double ligature close to the bifurcation; Group INTRAPERITONEAL (IPI; n=8) received IUI into the right uterine horn, the left horn was closed by double ligature and semen was deposited intraperitoneally at the surface of the left ovary. Genital tracts were removed 65-66 h after hCG, the oviducts were flushed and ova (n=299) were analyzed for fertilization and cleavage. Furthermore, the accessory spermatozoa count/oocyte was graded as 0, without spermatozoa, 1, <5 spermatozoa, 2, 5-50 spermatozoa, 3, 50-100 spermatozoa and 4, >100 spermatozoa. The results indicate that low dose IUI into one horn provides a lower grade of accessory spermatozoa in the contra-lateral side (1.6 vs. 2.8). No spermatozoa were found in ova flushed from oviducts of the ligated uterine horn, even after intraperitoneal insemination (P<0.05), and no fertilization occurred, respectively. Our results clearly indicate that after low dose IUI into one uterine horn, spermatozoa reach the contralateral oviduct via transuterine migration.  相似文献   

Antagonisten und Pr?datoren der Maulbeerschildlaus Pseudaulacaspis pentagona (Targioni-Tozzetti, 1886) (Homoptera: Diaspididae) in Baden-W��rttemberg     

Helmut Rauleder 《Erwerbs-Obstbau》2011,53(2):51-58

Antagonists of the white scale (Pseudaulacaspis pentagona, Targioni-Tozzetti, 1886) were determined from peaches and sweet cherries as host plant at the orchards of the Centre for Agriculture and Technology Augustenberg (LTZ) in 2009 and 2010 as well as from four other commercial red and black currant orchards in Baden-Wuerttemberg in 2010. The study focused mainly on the parasitoid species of P. pentagona as well as the rate of parasitism of white scale caused by these parasitoids using emergence traps with invested branch samples of the different host plants in the laboratory. Beside the parasitoids, possible predators of the white scale were observed in the field in 2010 at the orchard of the LTZ. The two parasitic wasp species Aphytis diaspidis and Encarsia berlesei (Howard, 1881 and 1906) were determined as parasitoids of the white scale, whereas A. diaspidis was dominant at most sites. Rate of parasitism caused by both species ranged from 1.9 to 23.0%. The Kidney-Spot Ladybird Beetle (Chilocorus renipustulatus, Scriba, 1790) could be observed as a predator of the white scale in the field.  相似文献   

Beobachtungen zum Flugverlauf des Pfirsichwicklers (Cydia molesta) – Vergleich der Effizienz von Pheromonfallen im Steinobst     

Helmut Rauleder 《Erwerbs-Obstbau》2007,49(1):18-22

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Assessment of the posterior segment of the cat eye by optical coherence tomography (OCT)     

Gekeler F  Gmeiner H  Völker M  Sachs H  Messias A  Eule C  Bartz-Schmidt KU  Zrenner E  Shinoda K 《Veterinary ophthalmology》2007,10(3):173-178

OBJECTIVES: To assess the feasibility of optical coherence tomography (OCT) for examining the cat ocular fundus, to provide normative data on retinal thickness in different fundus regions, and to demonstrate selected surgically induced vitreoretinal pathologies in the cat. ANIMAL STUDIED: Forty-five eyes of 28 healthy domestic cats and two eyes of domestic cats that had undergone subretinal implantation surgery for a visual prosthesis were examined. PROCEDURES: An optical coherence tomograph (Zeiss-Humphrey) was used to examine the anesthetized animals. At least five vertical and five horizontal scans in regular distribution were recorded for each cat including (1) the peripapillary region, (2) the area centralis, and (3) the peripheral retina. Thickness was measured manually at five locations in each scan. Retinal thickness was compared in the three above-mentioned fundus regions, between eyes and between vertical and horizontal scans. OCT was additionally performed in animals with retinal detachment and a subretinal visual prosthesis. RESULTS: OCT measurements required only minimal adjustments of human settings and yielded high quality images. In comparison to humans intraretinal layers were more difficult to differentiate. Retinal thickness was highest in the peripapillary region (245 +/- 21 microm), followed by the peripheral retina (204 +/- 11 microm) and the area centralis (182 +/- 11 microm; all P < 0.0001). There was no statistically significant difference between right and left eye or between vertical and horizontal scans. OCT demonstrated retinal detachment, an iatrogenic break and a subretinal prosthetic device in high detail. CONCLUSIONS: Retinal thickness was measurable with high precision; values compare well to older histologic studies. OCT bears significant advantages over histology in enabling one to repeat measurements in living animals and thus allowing longitudinal studies. Various vitreoretinal pathologies common in feline eyes are detectable and quantifiable by OCT.  相似文献   

Microarray based study on virulence-associated genes and resistance determinants of Staphylococcus aureus isolates from cattle     

Monecke S  Kuhnert P  Hotzel H  Slickers P  Ehricht R 《Veterinary microbiology》2007,125(1-2):128-140

Staphylococcus aureus is a common pathogen which can colonise and infect not only man, but also domestic animals. Especially, infection of cattle is of high economic relevance as S. aureus is an important causal agent of bovine mastitis. In the present contribution, a DNA microarray was applied for the study of 144 different gene targets, including resistance genes and genes encoding exotoxins, in S. aureus isolated from cows. One hundred and twenty-eight isolates from Germany and Switzerland were tested. These isolates were assigned to 20 different strains and nine clonal complexes. The majority of isolates belonged either to apparently closely related clonal complexes 8, 25, and 97 (together 34.4%) or were related to the sequenced bovine strain RF122 (48.4%). Notable characteristics of S. aureus of bovine origin are the carriage of intact haemolysin beta (in 82% of isolates tested), the absence of staphylokinase (in 89.1%), the presence of allelic variants of several exotoxins such as toxic shock syndrome toxin and enterotoxin N, and the occurrence of the leukocidin lukF-P83/lukM (in 53.1%). Two isolates were methicillin-resistant S. aureus (MRSA). One of them was a clonal complex 8 MRSA related to the epidemic MRSA strain Irish 01. The other one belonged to ST398/spa-type 34 resembling a newly emerging MRSA strain which has been described to occur in humans as well as in domestic animals. The presence of these two strains highlights the possibility of transfers of S. aureus strains between different host species.  相似文献   

煤矸石充填复垦地理化特性与重金属分布特征   总被引：15，自引：8，他引：7  

徐良骥  黄璨  章如芹  刘会平  严家平  Helmut Meuser  Lutz Makowsky 《农业工程学报》2014,30(5):211-219

为研究煤矸石充填复垦地农作物产量达到最佳时的覆土厚度及煤矸石充填复垦地重金属分布状况,该文以新庄孜矿煤矸石充填复垦地作为研究对象,采取室外田间小区试验和室内试验分析方法,选取不同覆土厚度50~60,60~70,70~80,80~90 cm的4类试验地块及对照地块对其土壤理化性质及农作物根系分布、长势产量、重金属元素含量进行调查分析,发现复垦地块各项指标与对照地块差异显著,劣于对照地块;煤矸石充填基质土壤理化性质较差;70~80 cm的厚度是煤矸石充填复垦造地较经济合理的覆土厚度。煤矸石复垦地重金属元素有向上迁移的趋势;复垦土壤及煤矸石基质中Pb、Zn、Cr元素含量处于安全水平;大部分监测的Cu、Ni、Cd元素含量高于淮南土壤背景值,低于中国土壤环境质量标准二级标准,对复垦土壤已构成潜在的威胁性,应予以重视;覆土厚度50~60 cm地块的煤矸石基质中Cu、Ni元素含量处于安全水平,煤矸石基质中Cd元素含量高于淮南土壤土壤背景值,低于中国土壤环境质量标准二级标准,应予以重视。结果表明70~80 cm的厚度是煤矸石充填复垦造地最佳覆土厚度;新庄孜煤矸石充填复垦地重金属有向上迁移的趋势,复垦土壤中重金属含量符合国家二级标准,未出现重金属污染情况,但部分重金属元素含量高于淮南市土壤背景值,应予以重视。  相似文献   

Brownian motion of DNA confined within a two-dimensional array     

Nykypanchuk D  Strey HH  Hoagland DA 《Science (New York, N.Y.)》2002,297(5583):987-990

Linear DNA molecules are visualized while undergoing Brownian motion inside media patterned with molecular-sized spatial constraints. The media, prepared by colloidal templating, trap the macromolecules within a two-dimensional array of spherical cavities interconnected by circular holes. Across a broad DNA size range, diffusion does not proceed by the familiar mechanisms of reptation or sieving. Rather, because of their inherent flexibility, DNA molecules strongly localize in cavities and only sporadically "jump" through holes. Jumping closely follows Poisson statistics. By reducing DNA's configurational freedom, the holes act as molecular weight-dependent entropic barriers. Sterically constrained macromolecular diffusion underlies many separation methods and assumes an important role in intracellular and extracellular transport.  相似文献