Spinal tumoral calcinosis is reported in a Berner sennenhund puppy. The condition was manifested clinically as a non-ambulatory tetraparesis associated with neck pain. On survey radiographs there was a focal calcified mass at the atlantoaxial articulation. Computed tomography and computed tomographic myelography gave additional information on the extent of the mass and on the degree of spinal cord compression. The mass was removed surgically and the dog made a complete recovery. 相似文献
We conducted two studies on how highways affect their adjacent habitats by sampling carabid beetles (Coleoptera, Carabidae)
in patches of formerly continuous forest next to highways. (1) We sampled carabids at 14 highway intersections near Helsinki,
Finland. Each intersection (constructed 2–40 years ago) had two forested patches to study: a remnant (0.5–37.4 ha) and, isolated
from the remnant by an intersection lane, an islet (size 0.2–1.8 ha). Pitfall trap catch data (2301 carabids, 25 species)
showed that remnants hosted higher catches of three carabid species, and slightly higher species richness, than islets (patch-size
effect). Time since intersection construction had no apparent effect on carabids. Traffic volume along the intersection lane
determined the assemblage structure of carabids in dry patches, and the abundance of a forest carabid Calathus micropterus. Compared to moist patches, drier patches hosted lower catches of four generalist species; they also had different assemblages
of carabids (habitat-type effect). An interaction between patch size and habitat type for a forest generalist Pterostichus oblongopunctatus indicated that the patch-size effect was dependent on habitat type. (2) We examined possible dispersal of carabids among
forested patches that were separated by highway lanes in Drenthe, the Netherlands. We released 2696 marked individuals of
10 species, and recaptured 376 using dry pitfall traps. We found no evidence for inter-patch movement for nine forest species,
but 22 of 225 recaptured individuals of Poecilus versicolor, an eurytopic open-habitat species, had crossed the highway. Catches of seven forest species were also significantly lower
in the road verges, compared to the adjacent forests. These two studies suggest that (i) decreasing patch size negatively
affects forest-carabid catch and overall species richness, (ii) habitat type can affect the intensity of the patch-size effect,
(iii) carabid assemblages of forest fragments vary with traffic volume (which may be linked with urbanization), (iv) forest
carabids rarely cross highways, and (v) open habitats associated with road margins are dispersal barriers for forest carabids. 相似文献
Summary This study examines the effects of random cross-fertilization on genotypic frequencies during the development of lines by the method of single seed descent, using a combination of generation matrix methods and equilibrium prediction equations for two linked loci. Results show that heterozygosity declines rapidly in early generations, as in the case of complete selfing, and declines to an equilibrium limit that is relatively insensitive to the degree of linkage and almost entirely determined by the frequency of crossing. The overall frequency of recombinant homozygotes is reduced by crossing but the frequency increases in successive generations to an equilibrium limit which is independent of the level of linkage and depends entirely on the frequency of crossing. The approach to the limiting frequency of recombinant homozygotes is extremely slow, in terms of generations, for closely linked loci and low frequency of crossing. The study then concentrates on the frequency structure of the F8 generation to cover the time-span typically involved in practical breeding programmes. 相似文献
As part of the 2015 Paris climate agreement and under its Green Deal, the EU proposes to strongly rely on forests for offsetting its carbon footprint. However, planting trees should be avoided in wildfire prone and drought prone habitats, which are expanding significantly as climate warms across Europe. In favorable habitats, tree planting remains a controversial solution and the risk of using inappropriate material is high in the absence of long-term planning, unfortunately typical of the forest seed and nursery sector. The EU forest tree planting strategy should pay close attention to local land-use issues, to within- and among-species genetic diversity and should adopt relevant, pluri-annual funding schemes and planting contracts rather than letting market opportunities govern the future of forest tree plantations.
Plant growth response to salinity on a scale of years has not been studied in terms of growth analysis. To gain insights into this topic, 2-year-old Mediterranean Fan Palm (Chamaerops humilis L.) and Mexican Fan Palm (Washingtonia robusta H. Wendl) seedlings, each with its own distinct plant morphology, were grown for 2 years in a peat soil and irrigated with water of 2 dS m(-1) (control) or 8 dS m(-1) (saline). Plants were harvested on seven occasions and the time trends in relative growth rate (RGR, the rate of increase of biomass per unit of biomass already existing) and its components were analysed. In the long term, salinity produced a slight reduction in the mean RGR, values in both species. In the short term, salinity caused a reduction in RGR. However, during the second year, plants irrigated with 8 dS m(-1) grew somewhat more quickly than the control plants, probably as a result of delay in the growth kinetics due to salinity. Regarding RGR components, leaf nitrogen productivity (the rate of biomass gain per unit leaf N and time) was the major factor causing the differences in RGR resulting from salinity. Washingtonia robusta showed a relatively high plasticity in plant morphology by increasing root and decreasing stem biomass allocation in the presence of salinity. However, the long-term response of W. robusta to salinity, based to a great extent, on this morphological plasticity, was less effective than that of C. humilis, which is based mainly on the contribution of leaf N to RGR values. 相似文献
It is commonly accepted that scrapie-resistance and -susceptibility in sheep are genetically controlled. Consequently, the selection of sheep with scrapie-resistant genotypes is currently one of the most important objectives of the sheep breeding associations. However, during the last two years, new data have become available on transmissible spongiform encephalopathy (TSE) cases in TSE-resistant sheep in several European Union member states. The present paper describes the first Belgian natural "atypical" TSE case in a sheep with a scrapie-resistant genotype (ARR/ARR) detected via active surveillance. No other infections or diseases were detected in the source flock. The continued finding of new "atypical" TSE cases in sheep with scrapie-resistant genotypes undermines the purpose and efficacy of the breeding programs. 相似文献
A highly sensitive and specific LC-MS/MS method for the quantitation of largazole thiol, the active species of the marine-derived preclinical histone deacetylase inhibitor, largazole (prodrug), was developed and validated. Largazole thiol was extracted with ethyl acetate from human or rat plasma along with the internal standard, harmine. Samples were separated on an Onyx Monolithic C18 column by a stepwise gradient elution with 0.1% formic acid in methanol and 0.1% aqueous formic acid employing multiple reaction monitoring (MRM) detection. Linear calibration curves were obtained in the range of 12.5–400 ng/mL with 200 µL of human plasma. The overall intra-day precision was from 3.87% to 12.6%, and the inter-day precision was from 7.12% to 9.8%. The accuracy at low, medium and high concentrations ranged from 101.55% to 105.84%. Plasma protein bindings of largazole thiol in human and rat plasma as determined by an ultrafiltration method were 90.13% and 77.14%, respectively. Plasma drug concentrations were measured by this LC-MS/MS method. The pharmacokinetics of largazole thiol in rats was studied following i.v. administration at 10 mg/kg and found to follow a two-compartment model. Largazole thiol was rapidly eliminated from systemic circulation within 2 h. The established LC-MS/MS method is suitable for the analysis of largazole thiol in human plasma, as well. 相似文献
Gilbert et al. (Reports, 28 September 2007, p. 1927) reported that "hair shafts surpass comparably stored bone as an aDNA source [...] in regard to preservation and concentration of mtDNA." When experimental parameters are carefully controlled for, including adequate sampling, quantitative polymerase chain reaction analysis, and modeling the decay of DNA, the general importance of this claim is not supported. 相似文献