Growth,Reproductive Performance,Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs     

Noritaka ADACHI  Daisuke YAMAGUCHI  Akiyuki WATANABE  Narumi MIURA  Seiji SUNAGA  Hitoshi OISHI  Michiko HASHIMOTO  Takatsugu OISHI  Masaki IWAMOTO  Hirofumi HANADA  Masanori KUBO  Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105

The objective of this study was to examine the health and meat production of cloned sows and their progenies in order to demonstrate the application of somatic cell cloning to the pig industry. This study compared the growth, reproductive performance, carcass characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies. We measured their body weight, growth rate and feed conversion and performed a pathological analysis of their anatomy to detect abnormalities. Three of the five cloned pigs were used for a growth test. Cloned pigs grew normally and had characteristics similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a Landrace boar and used for a progeny test. F1 progenies had characteristics similar to those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White boar and used for the progeny test. F2 progenies grew normally. There were no biological differences in growth, carcass characteristics and amino acid composition among cloned sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies showed normal reproductive performance. No specific abnormalities were observed by pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs had a normal karyotype. These results demonstrate that cloned female pigs and their progenies have similar growth, reproductive performance and carcass quality characteristics and that somatic cell cloning could be a useful technique for conserving superior pig breeds in conventional meat production.  相似文献   

Effects of high dose of vitamin A on reproduction and egg quality of Japanese flounder Paralichthys olivaceus   总被引：2，自引：0，他引：2  

Hirofumi Furuita  Hideki Tanaka  Takeshi Yamamoto  Manabu Shiraishi  Toshio Takeuchi 《Fisheries Science》2001,67(4):606-613

ABSTRACT: The present study was conducted to investigate the effects of dietary vitamin A on reproduction and egg quality in Japanese flounder Paralichthys olivaceus . Broodstock were fed experimental pellets containing two levels of vitamin A [11 × 10³ IU/100 g (control diet; CD), 337 × 10³ IU/100 g (experimental diet; ED)] for approximately 2 months before spawning and during the spawning period. Two groups of five females (average weight 1.4 kg) and 10 males (average weight 0.7 kg) were randomly allocated to two 30 m³ indoor tanks. Total egg production of the CD group was slightly higher than the ED group. Percentage of buoyant eggs and hatching rate of the ED group were significantly higher than the CD. In other egg quality parameters, such as percentage abnormal larvae and starvation tolerance of larvae, no notable difference was found between these two groups. At the end of the experiment, the skin color of broodstock in the ED group was darker than that of the CD group. Vitamin A content in eggs of the ED group was significantly higher than that of the CD group. However, the difference in vitamin A content in eggs between the ED and CD groups was much smaller than that in the liver of the females between the two groups. These results indicate that feeding broodstock a higher level of vitamin A increases the vitamin A content in eggs but does not affect egg quality in Japanese flounder because excess dietary vitamin A was stored mainly in the broodstocks' liver.  相似文献   

Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan     

Hiroya ITO  Kiyohito KATSURAGI  Shunsuke AKAMA  Hirofumi YUZAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):601-604

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Nutrient digestibility values of a test diet determined by manual feeding and self-feeding in rainbow trout and common carp     

Takeshi Yamamoto  Takao Shima  Hirofumi Furuita  Nobuhiro Suzuki  Manabu Shiraishi 《Fisheries Science》2001,67(2):355-357

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Effect of taurine supplemented practical diet on growth performance and taurine contents in whole body and tissues of juvenile Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>     

Shin-Kwon?Kim  Toshio?TakeuchiEmail author  Atsushi?Akimoto  Hirofumi?Furuita  Takeshi?Yamamoto  Masahito?Yokoyama  Yuko?Murata 《Fisheries Science》2005,71(3):627-632

ABSTRACT:   The effect of dietary taurine on juvenile Japanese flounder was determined by feeding three taurine-supplemented experimental diets (TAU) and a commercial diet (CD) to evaluate a practical diet for juvenile Japanese flounder. Juvenile Japanese flounder were reared on the three experimental diets supplemented with taurine at 0, 0.5, 1.0% and CD. These diets were fed to juvenile Japanese flounder of an initial mean body weight of 0.2 g for 6 weeks at 20°C and the taurine contents of the whole body and tissues were analyzed. The final average body weight of juvenile Japanese flounder fed the 1.0% TAU was significantly higher than that of the other groups. Taurine contents in the whole body and tissues increased with the increase in dietary taurine level. These results indicate that juvenile Japanese flounder require at least 15 mg/g taurine in the diet, even though a combined mix of fish, krill and squid meal was the main protein source in the experimental diets.  相似文献   

Aging of whiskey increases the potentiation of GABA(A) receptor response     

Koda H  Hossain SJ  Kiso Y  Aoshima H 《Journal of agricultural and food chemistry》2003,51(18):5238-5244

It is known that the target of most mood-defining compounds such as ethanol is an ionotropic gamma-aminobutyric acid receptor (GABA(A) receptor). The potentiation of the response of these inhibitory neurotransmitter receptors induces anxiolytic, sedative, and anesthetic activities in the human brain. Because both extracts of whiskey by pentane and fragrant components in whiskey potentiate the GABA(A) receptor-mediated response, GABA(A) receptors were expressed in Xenopus oocyte by injecting cRNAs prepared from the cloned cDNA for the alpha(1) and beta(1) subunits of the bovine receptors in order to study the effects of whiskey itself on the GABA(A) receptor-mediated response. Whiskey itself also potentiated the electrical responses of GABA(A) receptors generally more than ethanol at the same concentration as that of the whiskey. The potentiation of the GABA(A) receptor-mediated response increased with the aging period of the whiskey. Inhalation of whiskey to mice increased the sleeping time induced by pentobarbital more than that of the same concentration of ethanol as the whiskey. These results suggest that not only ethanol but also minor components in whiskey play an important role in the potentiation of GABA(A) receptor-mediated response and possibly the sedative effect of whiskey. Although the minor components are present in extremely small quantities compared with ethanol in alcoholic beverages, they may modulate the mood or consciousness of humans through the potentiation of the GABA(A) receptor response after absorption into the brain, because these hydrophobic compounds are easily absorbed into the brain across the blood-brain barrier and are several thousands times as potent as ethanol in the potentiation of the GABA(A) receptor-mediated response.  相似文献   

Effects of coffee components on the response of GABA(A) receptors expressed in Xenopus oocytes     

Hossain SJ  Aoshima H  Koda H  Kiso Y 《Journal of agricultural and food chemistry》2003,51(26):7568-7575

The effects of both coffee components and coffee extract on the electrical responses of GABA(A) receptors expressed in Xenopus oocytes were studied by injecting cRNAs of the alpha(1) and beta(1) subunits of the bovine receptors. The aqueous extract of coffee dose-dependently inhibited the GABA-elicited responses, whereas the lipophilic extract of coffee by diethyl ether slightly potentiated it at low doses (0.1-0.4 microL/mL) but showed inhibition at high doses (0.5-0.8 microL/mL). Theophylline inhibited the response in a noncompetitive mechanism (K(i) = 0.55 mM), whereas theobromine and trigonelline hydrochloride inhibited it in a competitive manner, K(i) = 3.8 and 13 mM, respectively. Benzothiazole, catechol, 2,4-dimethylstyrene, guaiacol, 1-octen-3-ol, sotolone, and 2,3,5-trimethylphenol potentiated the responses significantly. Potentiation elicited by guaiacol and sotolone was independent of GABA concentrations, whereas that by 1-octen-3-ol was dependent. When 1-octen-3-ol (100 mg/kg) was orally administered to mice prior to intraperitoneal administration of pentobarbital, the sleeping time of mice induced by pentobarbital increased significantly.  相似文献   

Experimental studies on sulfur dioxide injuries in higher plants     

Hirofumi Tanaka  Toshiharu Takanashi  Masaya Kadota  Michihiko Yatazawa 《Water, air, and soil pollution》1972,1(4):343-346

Photosynthesis is reversibly inhibited by exposing plants to SO₂. The formation of α-hydroxy sulfonate in the exposed plants is suggested as one of the effects, because this compound inhibits glycolic oxidase which is necessary in the glycolic acid pathway. The suppression of the glycolic acid path should affect various aspects of metabolism in plants, particularly the reduction of the biosynthesis of glycine and serine. In this paper, the biosynthesis of these amino acids in plants exposed to SO₂ was investigated to estimate the significance of the formation of α-hydroxy sulfonate in the plants. The results show that photosynthetic formation of serine was reduced in the exposed plants, and that α-hydroxy sulfonate was formed.  相似文献   

The Sequence Variations of Intron-3 of the α-Amylase Gene in Adzuki Bean   总被引：1，自引：0，他引：1  

JIN Wen-lin  Yamaguchi Hirofumi  Isigami Matiko  Yasuda Kentaro 《中国农业科学(英文版)》2003,2(10)

This study describes variation of intron-3 of a-amylase gene from 156 breeds of adzuki beansusing SSCP(single-strand conformation polymorphism)analysis. Based on a-amylase gene structure and se-quence, A pair of PCR primers, F (CCTACATTCTAACACACCCT) and R (GCATATTGTGCCAGTACAAT)were designed to amplify intron-3 fragments of a-amylase gene. 14 variant types were detected, including 13,9, 10, 4 variant types in the wild, weed, locally cultivated and modern brought-up adzuki beans respectively,9, 8, 7 variant types of the wild adzuki beans from Japan, China and Korea respectively, and some other va-riant types in the local adzuki beans from China and Bhutan. 60 % of subjects of cultivated races were found tobe EE type in the experiment. In addition, sequence analysis of intron-3 of α-amylase gene from 8 varianttypes reveals the evolution process of various variant types in adzuki beans.  相似文献   

Strength properties of glued laminated timber made from edge-glued laminae II: bending,tensile, and compressive strength of glued laminated timber     

Yasushi?HiramatsuEmail author  Kiyohiko?Fujimoto  Atsushi?Miyatake  Kenta?Shindo  Hirofumi?Nagao  Hideo?Kato  Hirofumi?Ido 《Journal of Wood Science》2011,57(1):66-70

The purpose of this study was to investigate the strength properties of glued laminated timber composed of edge-glued laminae and to investigate the influence of edge gluing on the strength properties. Glued laminated timber composed of multiple-grade laminae (symmetrical composition, strength grade E95-F270, 10 laminations) was produced from karamatsu (Larix kaempferi) edge-glued laminae according to the Japanese Agricultural Standard. The bending, tensile, and compressive strengths of the glued laminated timber were measured. The average bending, tensile, and compressive strengths were 33.4, 24.5, and 35.9 MPa, respectively, and these values are almost equal to those of glued laminated timber composed of karamatsu single-piece laminae. It was determined that finger-joints and knots in the edge-glued laminae played a significant role in the failure of specimens. However, the use of glued edge-joints did not appear to affect the failure of specimens.  相似文献