Detection of Pythium aphanidermatum in tomato using loop-mediated isothermal amplification (LAMP) with species-specific primers     

Shiro Fukuta  Reiko Takahashi  Satoru Kuroyanagi  Noriyuki Miyake  Hirofumi Nagai  Hirofumi Suzuki  Fujio Hashizume  Tomoko Tsuji  Hiromi Taguchi  Hideki Watanabe  Koji Kageyama 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):689-701

A loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA ITS sequence of P. aphanidermatum was developed. Results of a specificity test using 57 strains of Pythium spp. indicated that the LAMP assay gave no cross reactions in other 39 Pythium species, 11 strains of Phytophthora spp. and eight other soil borne pathogens. The detection limit was 10 fg of genomic DNA, which was ten times the sensitivity of the polymerase chain reaction. The LAMP assay was applied to hydroponic solution samples from tomato fields, and the results were compared to those of the conventional plating method. LAMP was observed to be effective for the specific detection of P. aphanidermatum. Furthermore, P. aphanidermatum was detected directly in tomato roots infected with P. aphanidermatum without DNA extraction. The LAMP method established in this study is a simple, sensitive and rapid tool for the detection of P. aphanidermatum.  相似文献   

Epicatechin-3-O-(3″-O-methyl)-gallate content in various tea cultivars (Camellia sinensis L.) and its in vitro inhibitory effect on histamine release     

Maeda-Yamamoto M  Ema K  Monobe M  Tokuda Y  Tachibana H 《Journal of agricultural and food chemistry》2012,60(9):2165-2170

It has been reported that epigallocatechin-3-O-(3″-O-methyl)-gallate (EGCG3″Me) and the EGCG3″Me-rich green tea ( Camellia sinensis L.) cultivar 'Benifuuki' exhibit antiallergic effects. The objective of this study was to investigate the effect of various tea leaf catechins on histamine release from murine bone marrow mast cells (BMMC). At a dose of 50 μg/mL, the rank order of histamine release inhibition was observed to be epicatechin-3-O-(3″-O-methyl)-gallate (ECG3″Me) > gallocatechin-3-O-(3″-O-methyl)-gallate (GCG3″Me) > EGCG3″Me > gallocatechin-gallate (GCG) > catechin-gallate (CG) > EGCG > epicatechin-gallate (ECG) > epigallocatechin (EGC) > gallocatechin (GC). Of the various tea cultivars analyzed by HPLC, the greatest content of ECG3″Me was found in the third crop of 'Benifuuki' (1.05% dry weight). Moreover, ECG3″Me content was positively correlated with EGCG3″Me content in 'Benifuuki' tea leaves. In an assay of mixtures of ECG3″Me and EGCG3″Me, inhibitory activity (50 μg/mL in total) was increased as the content of ECG3″Me increased. This suggests that ECG3″Me might link to the antiallergic effect of 'Benifuuki' tea, as has been reported for EGCG3″Me.  相似文献   

Effects of whey protein hydrolysate on growth promotion and immunomodulation in mouse pups in artificial rearing system     

Shiro Takeda  Akiko Harauma  Mariko Okamoto  Hirofumi Enomoto  Toshiji Kudo  Takehito Suzuki  Wataru Mizunoya  Toru Moriguchi 《Animal Science Journal》2020,91(1)

This study aimed to investigate the effects of whey protein hydrolysate (WPH) on the growth and immunity of mouse pups in artificial rearing (AR) system. Mouse pups were reared in the AR system with artificial milk including 5% WPH (AR with WPH) or not (AR without WPH), and the remaining pups were reared by their mother (dam) for 14 days after birth. The body weight change and body weight gain rates in the AR with WPH group were significantly higher than those observed in the AR without WPH group and similar to those in the dam group. Moreover the feed and protein efficiencies in the AR with WPH group were significantly higher than those of the AR without WPH group. In addition, the supplement of WPH in the AR system was shown to significantly elevate the number of CD3⁺CD8⁺, B220⁺CD19⁺, IA/IE⁺CD11c⁺, and CD11b⁺ in the thymocyte and/or splenocyte, and the thymus weight. Furthermore, MALDI‐TOF/MS analysis identified the amino acid sequences corresponding to some peptides, and indicated that VRTPEVDDE had the highest relative intensity among the peptides from tested WPH. Therefore, WPH would be required to not only promote growth, but also exert immunomodulatory activities in mouse pups in AR system.  相似文献   

Preface to the special section “Contamination of Agro-Environment and Forestry with Radionuclides from the Fukushima Daiichi Nuclear Power Station”     

Guest Editor-in-Chief of the Fukushima special section Masanori Saito  Guest Editors of the Fukushima special section Hirofumi Tsukada  Noriko Yamaguchi 《Soil Science and Plant Nutrition》2013,59(6):749-750

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Investigations on Cambodian soils     

Hideaki Saeki  Masao Okamoto  Junzo Azuma  Hirofumi Inoue  Masahiro Takiuchi  Hiroshi Tarumi 《Soil Science and Plant Nutrition》2013,59(1):16-22

Basic information on the nature of soils, their potentialities, and their limitations is necessary to evaluate Cambodian agriculture properly, and to develop a program for increasing food and other crops. Suitable information has, however, been limited, especially as there is not to be found in any report much data relating to soils of different origins over the whole country. There is, needless to say, some information on confined areas of Cambodia.  相似文献   

Iron-binding compounds produced by white-rot fungusPhanerochaete sordida YK-624     

Hirofumi Hirai  Aiko Onitsuka  Ryuichiro Kondo  Kokki Sakai  Tomoaki Nishida 《Journal of Wood Science》2001,47(5):374-377

Iron-binding compounds were isolated from a culture ofPhanerochaete sordida YK-624 and were found to bind to Fe(III) preferentially compared with Fe(II). Two iron-binding compounds were purified to near-homogeneity with gel permeation chromatography. Hydrolysis of the iron-binding compounds with 6N hydrochloric acid gave ninhydrin-negative products. The molecular weight of these compounds was 3–5 kDa. These compounds may play an important role in the reduction of extracellular manganese dioxide to Mn(II) by intracellular ferrireductases for lignin degradation by manganese peroxidase.  相似文献   

Glycation and phosphorylation of beta-lactoglobulin by dry-heating: effect on protein structure and some properties     

Enomoto H  Li CP  Morizane K  Ibrahim HR  Sugimoto Y  Ohki S  Ohtomo H  Aoki T 《Journal of agricultural and food chemistry》2007,55(6):2392-2398

Beta-lactoglobulin (beta-Lg) was glycated with maltopentaose and subsequently phosphorylated by dry-heating in the presence of pyrophosphate to investigate the structural and functional properties of phosphorylated beta-Lg. The circular dichroism spectra showed that the change of the secondary structure in the beta-Lg molecule by glycation and subsequent phosphorylation was small. The differential scanning calorimetry thermograms of beta-Lg showed that the denaturation temperature of the most stable domain was only slightly affected, whereas the retinol-binding activity of beta-Lg was somewhat reduced by glycation and subsequent phosphorylation. These results indicated that the conformational changes of the beta-Lg molecule by glycation and subsequent phosphorylation were mild. The anti-beta-Lg antibody response was somewhat reduced by glycation, but significant changes were not observed by phosphorylation. Although the stability of beta-Lg against heat-induced insolubility was improved by glycation alone, it was further enhanced by phosphorylation. The calcium phosphate solubilizing ability of beta-Lg was enhanced by phosphorylation following glycation.  相似文献   

Comparative proteomic analysis of liver mitochondrial proteins derived from cloned adult pigs reconstructed with Meishan pig fibroblast cells and European pig enucleated oocytes     

Takeda K  Tasai M  Iwamoto M  Oe M  Chikuni K  Nakamura Y  Tagami T  Nirasawa K  Hanada H  Pinkert CA  Onishi A 《The Journal of reproduction and development》2012,58(2):248-253

Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P<0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.  相似文献   

Nitrate reductase,a nitric oxide-producing enzyme: induction by pathogen signals   总被引：8，自引：1，他引：7  

Ayako?Yamamoto  Shinpei?Katou  Hirofumi?Yoshioka  Noriyuki?Doke  Kazuhito?KawakitaEmail author 《Journal of General Plant Pathology》2003,69(4):218-229

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Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia     

Walter MULEYA  Michihito SASAKI  Yasuko ORBA  Akihiro ISHII  Yuka THOMAS  Emiko NAKAGAWA  Hirohito OGAWA  Bernard HANG’OMBE  Boniface NAMANGALA  Aaron MWEENE  Ayato TAKADA  Takashi KIMURA  Hirofumi SAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):611-614

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