Iron-binding compounds produced by white-rot fungusPhanerochaete sordida YK-624     

Hirofumi Hirai  Aiko Onitsuka  Ryuichiro Kondo  Kokki Sakai  Tomoaki Nishida 《Journal of Wood Science》2001,47(5):374-377

Iron-binding compounds were isolated from a culture ofPhanerochaete sordida YK-624 and were found to bind to Fe(III) preferentially compared with Fe(II). Two iron-binding compounds were purified to near-homogeneity with gel permeation chromatography. Hydrolysis of the iron-binding compounds with 6N hydrochloric acid gave ninhydrin-negative products. The molecular weight of these compounds was 3–5 kDa. These compounds may play an important role in the reduction of extracellular manganese dioxide to Mn(II) by intracellular ferrireductases for lignin degradation by manganese peroxidase.  相似文献   

Glycation and phosphorylation of beta-lactoglobulin by dry-heating: effect on protein structure and some properties     

Enomoto H  Li CP  Morizane K  Ibrahim HR  Sugimoto Y  Ohki S  Ohtomo H  Aoki T 《Journal of agricultural and food chemistry》2007,55(6):2392-2398

Beta-lactoglobulin (beta-Lg) was glycated with maltopentaose and subsequently phosphorylated by dry-heating in the presence of pyrophosphate to investigate the structural and functional properties of phosphorylated beta-Lg. The circular dichroism spectra showed that the change of the secondary structure in the beta-Lg molecule by glycation and subsequent phosphorylation was small. The differential scanning calorimetry thermograms of beta-Lg showed that the denaturation temperature of the most stable domain was only slightly affected, whereas the retinol-binding activity of beta-Lg was somewhat reduced by glycation and subsequent phosphorylation. These results indicated that the conformational changes of the beta-Lg molecule by glycation and subsequent phosphorylation were mild. The anti-beta-Lg antibody response was somewhat reduced by glycation, but significant changes were not observed by phosphorylation. Although the stability of beta-Lg against heat-induced insolubility was improved by glycation alone, it was further enhanced by phosphorylation. The calcium phosphate solubilizing ability of beta-Lg was enhanced by phosphorylation following glycation.  相似文献   

Comparative proteomic analysis of liver mitochondrial proteins derived from cloned adult pigs reconstructed with Meishan pig fibroblast cells and European pig enucleated oocytes     

Takeda K  Tasai M  Iwamoto M  Oe M  Chikuni K  Nakamura Y  Tagami T  Nirasawa K  Hanada H  Pinkert CA  Onishi A 《The Journal of reproduction and development》2012,58(2):248-253

Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P<0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals.  相似文献   

Nitrate reductase,a nitric oxide-producing enzyme: induction by pathogen signals   总被引：8，自引：1，他引：7  

Ayako?Yamamoto  Shinpei?Katou  Hirofumi?Yoshioka  Noriyuki?Doke  Kazuhito?KawakitaEmail author 《Journal of General Plant Pathology》2003,69(4):218-229

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Molecular Epidemiology of Paramyxoviruses in Frugivorous Eidolon helvum Bats in Zambia     

Walter MULEYA  Michihito SASAKI  Yasuko ORBA  Akihiro ISHII  Yuka THOMAS  Emiko NAKAGAWA  Hirohito OGAWA  Bernard HANG’OMBE  Boniface NAMANGALA  Aaron MWEENE  Ayato TAKADA  Takashi KIMURA  Hirofumi SAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(4):611-614

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Muscle fibre distribution in forelimb,hindlimb and trunk muscles in three bat species: The little Japanese horseshoe,greater horseshoe and Egyptian fruit     

Claudius Luziga  Hirofumi Miyata  Hiroshi Nagahisa  Takashi Oji  Naomi Wada 《Anatomia, histologia, embryologia》2021,50(4):685-693

This study characterised muscle fibres in trunk, forelimb and hindlimb muscles of three bat species: little Japanese horseshoe (Rhinolophus cornutus), greater horseshoe (Rhinolophus ferrumequinum) and Egyptian fruit (Rousettus aegyptiacus). Twenty-seven muscles from trunk, forelimb and hindlimb were dissected, weighed and analysed by immunohistochemistry and sodium didecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and determined their cross-sectional areas (CSA). Results showed that Type IIa and Type IIa/x made the highest proportion of total muscle mass. Moderate proportion was formed by Type IIb. Type I and IIx appeared at very low levels in all bats. Type IIb was the only fibre type detected in patagial muscles in wing membrane of greater horseshoe while other fibre types were not observed. Type I muscle fibres were very few and appeared infrequently in fifteen muscles of Egyptian fruit and in only one muscle in each, greater horseshoe and little Japanese horseshoe. Type IIx was also detected in three muscles in greater horseshoe and only one muscle in Egyptian fruit but none in little Japanese horseshoe. The highest average CSA μm² was detected in Type IIb and values were 734.2μm² for LHB; 1537.9μm² for GHB and 1,720.9μm² for EFB. Lowest and undetermined values were observed for Type I and IIx. These data demonstrate that Type IIa, IIa/x and IIb form significant proportion of adult bat muscle mass and Type IIb is the largest fibre type. The distribution pattern is suggestive of specialised functions of the fibres in relation to orientation and speed of bats during flight.  相似文献   

Databases for technical aspects of immunohistochemistry: 2021 update     

Takayasu Moroki  Saori Matsuo  Hirofumi Hatakeyama  Seigo Hayashi  Izumi Matsumoto  Satoshi Suzuki  Takashi Kotera  Kazuyoshi Kumagai  Kiyokazu Ozaki 《Journal of toxicologic pathology》2021,34(2):161

With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.  相似文献   

Experimental studies on SO2 injuries in higher plants III: Inhibitory effect of suite ion on 14 CO 2 fixation     

Hirofumi Tanaka  Toshiharu Takanashi  Michihiko Yatazawa 《Water, air, and soil pollution》1974,3(1):11-16

Photosynthesis decreases reversibly in plants exposed to SO₂. Photosynthesis recovers when the exposure to SO₂ is discontinued. Inactivation of a photosynthetic enzyme, ribulose-1,5-diphosphate carboxylase, by sulfonation of its SH groups was investigated as a cause of the reversible reduction of photosynthesis. The relationship between the sulfite ion concentration in the reaction mixture and ¹⁴CO₂ fixation catalized by the enzyme which was prepared from alfalfa leaves was explored by using radioactive NaHCO₃. About 50% and 85% inhibitions of ¹⁴CO₂ fixation were observed at 3 × 10^?3 M and 3 × 10^?2 M concentration of sulfite ion in the reaction mixture, respectively. The accumulation of 3 × 10^?4 M sulfite ion on the reaction site of the enzyme involved in the plants which were exposed to SO₂ could considerably reduce the CO₂ assimilation of the plant.  相似文献   

Effect of oral administration of a single bolus of six different protein sources on digestive physiology of red seabream Pagrus major juveniles     

Murashita  Koji  Takakuwa  Fumiaki  Matsunari  Hiroyuki  Yoshinaga  Hazuki  Yamamoto  Takeshi  Oku  Hiromi  Furuita  Hirofumi 《Fish physiology and biochemistry》2022,48(4):939-954

Fish Physiology and Biochemistry - To reveal direct effects of various protein sources on digestive physiology of red seabream, Pagrus major (38.5 ± 0.4&nbsp;g), six different...  相似文献   

Induction of centrum defects in amberjack Seriola dumerili by exposure of embryos to hypoxia     

Yoshifumi Sawada  Manabu Hattori  Masahide Iteya  Yasuaki Takagi  Kazuhiro Ura  Manabu Seoka  Keitaro Kato  Michio Kurata  Hirofumi Mitatake  Shigekazu Katayama  Hidemi Kumai 《Fisheries Science》2006,72(2):364-372

ABSTRACT:   Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili . Firstly, 7-somite stage embryos were exposed to waters of 0, 12.5, 25, 50, and 100% dissolved oxygen (DO) for 0.5, 1, 2, 3, 4, and 5 h to confirm the effective dose (DO concentration and duration of exposure) of hypoxia that induces somitic disturbances in newly hatched larvae. Exposure of embryos to 12.5% DO concentration for longer than 0.5 h induced somitic disturbances. Following this result, centrum defects in juveniles were investigated by an induction experiment with embryos exposed to 12.5% DO for 2 h at the gastrula, 1- or 2-somite, 10-somite, 15-somite, or heart beating stage. This experiment revealed that centrum defects were induced only during somitogenesis, and somitic disturbances were the premonitory symptom of centrum defects. These results indicate hypoxia during somitogenesis as a possible cause of centrum defects in amberjack.  相似文献