Induction of Beige-Like Adipocytes in 3T3-L1 Cells     

Hiroki ASANO  Yohei KANAMORI  Satoshi HIGURASHI  Takayuki NARA  Ken KATO  Tohru MATSUI  Masayuki FUNABA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):57-64

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Characterization of Murine Pituitary-Derived Cell Lines Tpit/F1, Tpit/E and TtT/GF     

Saishu YOSHIDA  Masashi HIGUCHI  Hiroki UEHARU  Naoto NISHIMURA  Mitsuyoshi TSUDA  Hideji YAKO  Mo CHEN  Hideo MITSUISHI  Yoshiya SANO  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2014,60(4):295-303

The pituitary is an important endocrine tissue of the vertebrate that produces and secretes many hormones. Accumulating data suggest that several types of cells compose the pituitary, and there is growing interest in elucidating the origin of these cell types and their roles in pituitary organogenesis. Therein, the histogenous cell line is an extremely valuable experimental tool for investigating the function of derived tissue. In this study, we compared gene expression profiles by microarray analysis and real-time PCR for murine pituitary tumor-derived non-hormone-producing cell lines TtT/GF, Tpit/F1 and Tpit/E. Several genes are characteristically expressed in each cell line: Abcg2, Nestin, Prrx1, Prrx2, CD34, Eng, Cspg4 (Ng2), S100β and nNos in TtT/GF; Cxcl12, Raldh1, Msx1 and Twist1 in Tpit/F1; and Cxadr, Sox9, Cdh1, EpCAM and Krt8 in Tpit/E. Ultimately, we came to the following conclusions: TtT/GF cells show the most differentiated state, and may have some properties of the pituitary vascular endothelial cell and/or pericyte. Tpit/F1 cells show the epithelial and mesenchymal phenotypes with stemness still in a transiting state. Tpit/E cells have a phenotype of epithelial cells and are the most immature cells in the progression of differentiation or in the initial endothelial-mesenchymal transition (EMT). Thus, these three cell lines must be useful model cell lines for investigating pituitary stem/progenitor cells as well as organogenesis.  相似文献   

Comparison of cytokine mRNA expression in peripheral CD4+, CD8+ and γδ T cells between healthy Holstein and Japanese Black calves     

Hiromichi Ohtsuka  Hiroki Kobayashi  Kumi Kinouchi  Miki Kiyono  Yousuke Maeda 《Animal Science Journal》2014,85(5):575-580

Japanese Black (JB) calves are more susceptible to infectious diseases compared to Holstein (Hol) calves. To clarify the immunological differences between JB and Hol calves, expression of cytokine messenger RNA (mRNA) was examined using peripheral CD4⁺, CD8⁺ and γδ T cells. Healthy calves, 24 from each species, were examined. Blood samples were obtained from calves at 1 week, 1 month and 3 months old, eight calves for each age of each species. Peripheral blood mononuclear cells were stimulated with phytohemagglutin (PHA), and T cell subsets were isolated by positive selection using magnetic cell sorting (MACS). Levels of interlekin (IL)‐2, IL‐4, IL‐10 and interferon (IFN)‐γ mRNA in three T cell subsets were analyzed. WC1‐N1⁺ γδ T cell percentages were significantly lower in JB calves at 1 week and 1 month of age compared to Hol calves. In addition JB calves had significantly lower IL‐2, IL‐10 and IFN‐γ mRNA in WC1‐N1⁺ γδ T cells at 1 and 3 months of age, whereas there were no significant differences in cytokine mRNA of CD4⁺ and CD8⁺ cells between the two groups. Decreased cytokine mRNA and cell number of peripheral γδ T cells may affect negatively on the immune system of JB calves.  相似文献   

A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO     

Ashida H  Saito Y  Kojima C  Kobayashi K  Ogasawara N  Yokota A 《Science (New York, N.Y.)》2003,302(5643):286-290

The genomes of several nonphotosynthetic bacteria, such as Bacillus subtilis, and some Archaea include genes for proteins with sequence homology to the large subunit of ribulose bisphosphate carboxylase/oxygenase (RuBisCO). We found that such a RuBisCO-like protein (RLP) from B. subtilis catalyzed the 2,3-diketo-5-methylthiopentyl-1-phosphate enolase reaction in the methionine salvage pathway. A growth-defective mutant, in which the gene for this RLP had been disrupted, was rescued by the gene for RuBisCOfrom the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis.  相似文献   

Effects of the supplementation of a calcium soap containing medium-chain fatty acids on the fecal microbiota of pigs,lactating cows,and calves     

Hiroki Matsui  Taichi Imai  Makoto Kondo  Tomomi Ban-Tokuda  Yutaka Yamada 《Animal Science Journal》2021,92(1):e13636

Medium-chain fatty acids (MCFAs) have antialgal, antibacterial, antifungal, antiprotozoan, and antiviral activities. However, antibacterial activities of MCFAs in the hindgut of pigs and cattle are still unknown. We report the effects of the supplementation of MCFAs on fecal bacteria of pigs, lactating cows, and Japanese Black calves. Lactobacillus spp., Bifidobacterium spp., eaeA(+) Escherichia coli, Salmonella spp., Campylobacter jejuni, and Clostridium perfringens in the feces of animals were quantified by real-time PCR assay. There was no significant increase or decrease in Lactobacillus spp. and Bifidobacterium spp. in the three animals. In the pig feces, eaeA(+) E. coli was reduced to less than a third in the treatment group (P < 0.01). C. jejuni in the pig feces was also significantly less in the treatment group compared with the control (P < 0.01). In the lactating cow, eaeA(+) E. coli was reduced to one fifth of that in the control (P < 0.01). Salmonella spp. was halved in calf feces (P < 0.01). Thus, a reduction in Gram-negative pathogenic bacteria was observed. In conclusion, supplementation of a MCFA calcium soap in the diet would be beneficial to growing pigs, lactating cow, and calves by reducing pathogenic bacteria.  相似文献   

Comparison of extractive components in muscle and liver of three Loliginidae squids with those of one Ommastrephidae species     

Yoko Kani  Naoko Yoshikawa  Shigeru Okada  Hiroki Abe 《Fisheries Science》2007,73(4):940-949

Extractive components were determined for the mantle muscle and liver of three species of Loliginidae squid, Sepioteuthis lessoniana, Loligo bleekeri, and L. edulis, and one species of Ommastrephidae, Todarodes pacificus, as a control. Total free amino acid levels and the major free amino acids in muscle, taurine, proline, glycine, alanine, and arginine, were significantly higher in Loliginidae squids than in T. pacificus. The main nucleotide was adenosine 5′-monophosphate, which did not differ significantly between species. Malate was the organic acid found in muscle in highest concentrations. The muscle of these species contained a large amount of trimethylamine oxide. A large amount of glycine betaine was also detected in the muscle, but showed no large species difference. From these results, the muscle of Loliginidae species is considered to have a much sweeter taste than that of T. pacificus. Compared with muscle, squid liver was characteristic with high contents of taurine, glutamate, bitter amino acids, succinate, propionate, trimethylamine, and glycine betaine, and with low contents of sweet amino acids, arginine, nucleotides, malate, and trimethylamine oxide. These results suggest that squid liver is characterized by a complicated taste containing umami, bitterness, sourness, fishy flavor, and less sweetness.  相似文献   

Use of GIS and machine learning to predict disease in shrimp farmed on the east coast of the Mekong Delta,Vietnam     

Khiem  Nguyen Minh  Takahashi  Yuki  Yasuma  Hiroki  Oanh  Dang Thi Hoang  Hai  Tran Ngoc  Ut  Vu Ngoc  Kimura  Nobuo 《Fisheries Science》2022,88(1):1-13

Diseases in shrimp farms in the Mekong Delta of Vietnam cause significant crop losses and are therefore of great concern to producers. Once a pond becomes infected, it is difficult to prevent spread of the disease to nearby shrimp farming areas. Thus, predicting the occurrence of disease is an essential part of reducing the risk for shrimp farmers. In this study, we applied an integrated geographic information system and machine learning system to predict three serious diseases of shrimp, namely, acute hepatopancreatic necrosis, white spot syndrome disease, and Enterocytozoon hepatopenaei infection, based on data collected from shrimp farms in the Tra Vinh, Bac Lieu, Soc Trang, and Ca Mau provinces of Vietnam. We first constructed a map showing the distribution of these diseases using the locations of affected farms, and then we conducted spatial analysis to acquire the geographical features of the affected locations. This latter information was combined with environmental factors and clinical signs to form the set of independent variables affecting the outbreak of diseases. The neural network model outperformed the logistic regression, random forest, and gradient boosting methods in terms of predicting infection to estimate the probability of disease occurrence in farmed areas. Acute hepatopancreatic necrosis disease infected farms downstream of the Co Chien and Hau Rivers of Tra Vinh and west of Ca Mau. Enterocytozoon hepatopenaei infection is distributed in Soc Trang Province, while white spot syndrome virus has spread to the coastal districts of Soc Trang and Bac Lieu Provinces, where it is highly associated to water from a complex canal system.

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Influence combinee des facteurs temperature et salinite sur la metamorphose et la croissance larvaire de la crevette rose Palaemon serratus (Pennant) (Crustacea,Decapoda, Palaemonidae)     

Hiroki Yagi  Hubert J Ceccaldi 《Aquaculture (Amsterdam, Netherlands)》1984,37(1):73-85

The rate of metamorphosis of larvae, duration of larval stages and carapace lengths of postlarvae were measured in the common prawn, Palaemon serratus, reared in 30 different combinations of temperature and salinity. Temperature varied from 13°C to 29°C and salinity from 13‰ to 43‰. When salinity.was 13‰, this species was able to metamorphose at a temperature of 21°C. For salinities of 19‰, 25‰, 31‰, 37‰ and 43‰, the temperature range over which metamorphosis occurs extends from 17°C to 25°C. The equation of Van't Hoff can be adapted to calculate the relation between the rearing rate, which is the converse of the duration of larval stages, and the water temperature. The Q₁₀ values vary from 1.39 minimum at a salinity of 37‰ to 2.28 maximum at 31‰. Neither the effect of temperature (at 0.1%) nor that of salinity (at 1%) is significant for the carapace lengths of postlarvae.Tridimensional models of the combined effects of temperature and salinity on the rate of metamorphosis and on the number of metamorphosed postlarvae appearing daily were constructed for this species.  相似文献   

Potato yield enhancement through intensification of sink and source performances     

Akira Katoh  Hiroki Ashida  Ichiro Kasajima  Shigeru Shigeoka  Akiho Yokota 《Breeding Science》2015,65(1):77-84

The combined total annual yield of six major crops (maize, rice, wheat, cassava, soybean, and potato; Solanum tuberosum L.) amounts to 3.1 billion tons. In recent years, staple crops have begun to be used as substitutes for fossil fuel and feedstocks. The diversion of crop products to fuels and industrial feedstocks has become a concern in many countries because of competition for arable lands and increased food prices. These concerns are definitely justified; however, if plant biotechnology succeeds in increasing crop yields to double the current yields, it will be possible to divert the surplus to purposes other than food without detrimental effects. Maize, rice, wheat, and soybean bear their sink organs in the aerial parts of the plant, and potato in the underground parts. Plants with aerial storage organs cannot accumulate products beyond their capacity to support the weight of these organs. In contrast, potato has heavy storage organs that are supported by the soil. In this mini-review, we introduce strategies of intensifying potato productivity and discuss recent advances in this research area.  相似文献   

Effects of long-term administration of carprofen on healing of a tibial osteotomy in dogs     

Ochi H  Hara Y  Asou Y  Harada Y  Nezu Y  Yogo T  Shinomiya K  Tagawa M 《American journal of veterinary research》2011,72(5):634-641

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