Evaluation of cytokine messenger RNA expression in peripheral blood mononuclear cells from dogs with canine demodicosis     

Tani K  Morimoto M  Hayashi T  Inokuma H  Ohnishi T  Hayashiya S  Nomura T  Une S  Nakaichi M  Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):513-518

Using RT-PCR and semi-quantitative PCR, mRNA expression for canine interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5, IL-10, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta in peripheral blood mononuclear cells (PBMCs) was examined in dogs with or without demodicosis. mRNA expression for IFN-gamma as well as TNF-alpha in dogs with demodicosis (localized (LD) and generalized (GD)) was slightly lower than those in dogs without demodicosis (healthy controls). Expression of IL-5 mRNA in dogs with demodicosis was higher than that in control dogs, but there were no significant differences in IL-4 and IL-10 mRNA expression levels among the three groups. On the other hand, expression levels of TGF-beta mRNA in dogs with GD were higher than those in control dogs and dogs with LD. The expression levels of IL-5 and TGF-beta mRNA decreased in all three dogs with GD which showed resolution of the clinical signs. Taken together, these results suggest that the Th2-like response in PBMCs from dogs with demodicosis is up-regulated, and that subsequent increased expression of IL-5 and TGF-beta mRNA in dogs with GD is reversible after treatment. Therefore, these cytokines, particularly IL-5, might be a useful clinical index of the clinical course in demodicosis. Also, increased TGF-beta mRNA expression might be a key factor for revealing the difference in the mechanism of onset between LD and GD.  相似文献   

Identification of Petunia hybrida cultivars that diurnally emit floral fragrances     

Kiriko Nakamura  Kiyoshi Matsubara  Hitoshi Watanabe  Hisashi Kokubun  Yoshihiro Ueda  Naomi Oyama-Okubo  Masayoshi Nakayama  Toshio Ando 《Scientia Horticulturae》2006

In order to identify genetic resources for breeding fragrant petunias for use as bedding plants, volatile compounds released by day from the flowers of 40 commercial Petunia hybrida cultivars were analyzed using a solid-phase micro-extraction technique coupled with GC–MS. The three cultivars with solid deep-blue flowers that accumulate malvidin in corollas with high tissue pH were found to emit abundant iso-eugenol as the principal floral fragrance. Several other cultivars that emitted considerable amounts of methylbenzoate and/or benzylbenzoate from the flower were also identified. Association between the floral fragrance and the other floral traits such as floral anthocyanin composition and corolla-tissue pH was discussed.  相似文献   

Experimental infections of a ciliate Tetrahymena pyriformis on ornamental fishes     

Aranya Ponpornpisit  Makoto Endo  Hisashi Murata 《Fisheries Science》2000,66(6):1026-1031

SUMMARY: Experimental infection of Tetrahymena pyriformis was conducted on ornamental fishes with skin wounded by acetic acid treatment. Among fishes used in this experimental infection, guppy Poecilia reticulata , pristella Pristella maxillaris , neontetra Paracheirodon innesi and cherry barbs Puntius titteya proved to be sensitive to challenge, while medaka Oryzias latipes , dwarf gourami Colisa lalia , goldfish Carassius auratus , platy Xiphophorus maculatus and angelfish Pterophyllum scalare were resistant. In catfish Corydoras aeneus infection was unsuccessful. Exposure to Tetrahymena at ≥100 cells/mL at 25 to 30°C and at pH of 6.0 to 8.0 resulted in the successful infection of some sensitive fish species. Histological and bacteriological observations suggest that deeply destroyed skin tissues from the acid-treated method are the primary factors in successful infection.  相似文献   

Simultaneous measurements of quantum yield and CO2 uptake for the assessment of non-assimilative electron flow in tree leaves     

Michito Tsuyama  Hajime Kobayashi  Masaru Shinya  Hisashi Yahata  Yoshichika Kobayashi 《Journal of Forest Research》1996,1(2):79-85

Using attached and detached leaves ofAcer palmatum Thunb. andRhaphiolepsis umbellata Makino, pulse-modulated chlorophyll fluorescence and CO₂ exchange were measured. Quantum yield of photosynthesis was determined from the fluorescence parameter(Fm′−Fs)/Fm′, where (Fm′−Fs) was defined as the difference between steady state chlorophyll fluorescence (Fs) and maximum fluorescence (Fm′) elicited by a saturating light pulse. The rate of electron transport through photosystem II (total electron flow) was calculated from the product of quantum yield andA (PFD), whereA is the rate of absorbed photons as given by leaf absorptance, and PFD is the photon flux density at the leaf surface. The rate of electron transport dependant on CO₂ uptake (assimilative electron flow) was calculated from the gross photosynthetic rate in a leaf. The difference between the rates of total and assimilative electron transport was denoted as the rate of non-assimilative electron transport which depends on photorespiration and oxygen reduction. Available data provided quantitative information on the rate of non-assimilative electron flow in intact leaves. When leaf photosynthesis ofA. palmatum was measured under sunlight, the rates of total and assimilative electron transport were determined to be approximately 900 and 150 μmol equiv. e/mg Chl·h, respectively. The difference (750 μmol equiv. e/mg Chl·h) was attributed to the activity of non-assimilative electron flow. The ratio of total to assimilative electron flow was found to increase gradually with rising in irradiance. The results suggest that non-assimilative electron flow occurred at much higher rate than assimilative electron flow at high irradiance. Implications of the results are briefly discussed in relation to photosynthesis limitation in tree leaves.  相似文献   

Effects of polyline simplification of dynamic GPS data under forest canopy on area and perimeter estimations     

Yasuyuki Tachiki  Tetsuhiko Yoshimura  Hisashi Hasegawa  Tomonori Mita  Tetsuro Sakai  Futoshi Nakamura 《Journal of Forest Research》2005,10(6):419-427

The objectives of this study were to determine Global Positioning System (GPS) positional errors while moving under the forest canopy and to clarify the effects of polyline simplification on area and perimeter estimations. We used the Pathfinder Pro XR and GPSMAP 76S, which are categorized as “high-end mapping” and “general navigation” GPS receivers, respectively. The field tests were conducted in both natural and plantation forests. The results showed that the Pathfinder Pro XR, which has better multipath rejection technology, worked well, especially in the plantation forest under unfavorable conditions of higher stand density. We used analysis of variance to clarify the effects of the receiver type, positioning mode, stand type, and polyline simplification method on area and perimeter estimations. The receiver type and positioning mode were found to be significant factors that affected area estimation. The Pathfinder Pro XR estimated the area more accurately than the GPSMAP 76S, and differential GPS estimated the area more accurately than autonomous GPS. With respect to the perimeter, the receiver type, positioning mode, and polyline simplification method were found to be significant factors. The results showed that perimeter estimation was improved by using the velocity filter, and further improved by using the velocity filter and Douglas-Peucker algorithm, especially when the Pathfinder Pro XR was used. The GPSMAP 76S estimated the perimeter accurately without any filtering because its default speed filter worked well, even though the GPSMAP 76S is a general navigation GPS receiver.  相似文献   

Accuracy of shear properties of wood obtained by simplified Iosipescu shear test     

Yoshitaka Kubojima  Hiroshi Yoshihara  Hisashi Ohsaki  Masamitsu Ohta 《Journal of Wood Science》2000,46(4):279-283

We examined the accuracy of the shear properties of wood by the Iosipescu shear test using specimens whose shape was simplified. Quartersawn boards of sitka spruce (Picea sitchensis Carr.) and shioji (Japanese ash,Fraxinus spaethiana Lingelsh.) were used. Two types of specimen for the Iosipescu shear test were compared: a standard specimen whose notch angle is 90° and a keyhole type specimen, which is more easily prepared than the standard type. The shear modulus, yield shear stress, and failure shear stress of the keyhole-type specimen were compared to those of the standard specimen. Shear stress analysis was conducted using the finite element method (FEM). The results obtained were as follows: (1) The failure pattern obtained by the simplified Iosipescu shear test was similar to that seen with the standard Iosipescu shear test. (2) The shear modulus, yield stress, and failure stress obtained by the simplified Iosipescu shear test coincided with those by the standard Iosipescu shear test. (3) The principal strain angle and principal stress angle of the simplified Iosipescu shear test were about 45°. (4) It is recognized that pure stress is applied to the strain-gauge regions in the simplified Iosipescu shear test, and it is expected that the shear properties are independent of the notch angle.  相似文献   

Age trends in the genetic parameters of wood density and the relationship with growth rates in hybrid larch (Larix gmelinii var. japonica × L. kaempferi) F1     

Takaaki Fujimoto  Kazuhito Kita  Kazuko Uchiyama  Makoto Kuromaru  Hisashi Akutsu  Kazuyuki Oda 《Journal of Forest Research》2006,11(3):157-163

Age trends in variance components and heritability of overall wood density, earlywood and latewood density, and latewood proportion were investigated in 29-year-old trees of 19 full-sib families of hybrid larch (Larix gmelinii var. japonica × Larix kaempferi) F₁. The age–age correlation and optimum selection age for these traits were also estimated and genetic and phenotypic correlations between wood density and radial growth rate were calculated for each growth ring. Intraring wood density data were obtained using X-ray densitometry. The coefficient of additive genetic variance was stable over all ages, whereas the coefficient of environmental variances gradually decreased with increasing age, resulting in increases in heritability estimates with age for overall density. The latewood proportion had the highest heritability estimates at all ages, ranging from 0.44 to 0.66. Overall density and its various components at 28 years of age showed strong genetic correlations with their respective traits at all younger ages. Optimum selection ages for the wood density traits ranged from 8 to 14 years, at which point maximum gain efficiencies per year were obtained. There were negative correlations between wood density and radial growth rate at early ages, although these relationships tended to be weaker with increasing age. These results suggest that selection at a young age is effective for wood density, but particular care must be taken in selecting trees with an improved radial growth rate because rapid growth will result in a low-density wood product, especially in the early growth period.  相似文献   

Hydration behavior and compressive strength of cement mixed with exploded wood fiber strand obtained by the water-vapor explosion process     

Yi Min Wei  Bunichiro Tomita  Yasushi Hiramatsu  Atsushi Miyatake  Tsuyoshi Fujii  Tomoyuki Fujii  Shuichiro Yoshinaga 《Journal of Wood Science》2003,49(4):317-326

Poor compatibility was found between exploded wood fiber strand (WFS) and cement due to the excessive presence of water-soluble degraded polysaccharides in extractives of exploded WFS obtained from weathered wood waste treated by the water-vapor explosion process (WVEP). This study presents some comparative results from a continuing investigation on the compressive strengths of exploded WFS–cement mixtures. Based on results previously obtained with the hydration test, the relation between hydration behavior and compressive strength of the mixture was explored. In addition, the effect of the curing age on compressive strength development of the mixture with selected additive chemicals was examined. The results supported the results of early studies with hydration tests indicating that adding MgCl₂ to the mixtures of exploded WFS mixed with quick-curing cement or ordinary Portland cement and a composite of MgCl₂ + CaO added to the mixture of exploded WFS and furnace-slag cement effectively improved the hydration behaviors; it greatly enhanced the compressive strengths of mixtures as well. Compressive strengths were strongly correlated to maximum hydration temperatures (T_max) of wood–cement mixtures influenced by the cement type, wood wastes (treated or not with WVEP), additive chemicals, and their content levels. The results also indicated that adding selected chemicals had no significant effect on compressive strength among the mixtures of exploded WFS mixed, respectively, with three types of cement at a curing age of 180 days. X-ray diffraction, scanning electron microscopy, and energy dispersive X-ray spectroscopy were used to identify the hydration products and to probe the element distribution of the mixture in the wood–cement interface zone from a fractured surface.Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002  相似文献   

Comparison of bacterial endotoxin lipopolysaccharide concentrations in the blood,ovarian follicular fluid and uterine fluid: a clinical case of bovine metritis     

Fumie MAGATA  Yoshikazu ISHIDA  Akio MIYAMOTO  Hidefumi FURUOKA  Hisashi INOKUMA  Takashi SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):81-84

We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis.  相似文献   

Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis     

Saori MIURA  Noriyuki HORIUCHI  Kotaro MATSUMOTO  Yoshiyasu KOBAYASHI  Shin-ichiro KAWAZU  Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):883-887

Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.  相似文献