Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli     

YAO Wei  YU Xue-wu  CAO Dong  ZHAO Xiao-tong  GU Gui-bo  CHEN Yao  ZHANG Lei  LIU Yao-chuan  FENG Jun-ke 《中国畜牧兽医》2016,43(11):3080-3084

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.  相似文献   

光源、性别及光处理时间对草地贪夜蛾趋光行为的影响     

张亚南  刘雨欣  李梦瑶  姚梦语  魏国树 《植物保护学报》2023,50(4):1010-1015

为研发高效、精准的监测防控设备,利用八臂行为选择装置系统测定不同光源、性别和光处理时间下草地贪夜蛾Spodoptera frugiperda的趋光行为。结果显示,在紫外光（波长365 nm）、紫光（波长420～430 nm）、蓝光（波长465～475 nm）、绿光（波长525～535 nm）、黄光（波长585～595 nm）和红光（波长655～665 nm）6种波长光源下,草地贪夜蛾混合种群（雌雄成虫比为1∶1）对紫外光光源的趋光选择率最高,为25.61%,显著高于其他处理,其次为紫光和蓝光光源,趋光选择率分别为16.30%和8.82%,对红光的趋光选择率最低,为4.76%。在紫外光刺激下,草地贪夜蛾雌成虫的趋光选择率显著高于混合种群（雌雄成虫比为1∶1）,在红光刺激下,雄成虫的趋光选择率显著高于雌成虫。另外,在紫外光刺激下,光处理时间60 min草地贪夜蛾雌成虫的趋光选择率显著高于其他处理。表明光源与性别因素均影响草地贪夜蛾的趋光行为,光处理时间对草地贪夜蛾的趋光行为影响较小,且与光源及性别因素有关。  相似文献   

覆盖方式对旱地小麦花后旗叶抗氧化生理及粒重形成的影响     

张姚  程宏波  杨佳佳  马建涛  柴守玺  纪文宁  张仕林 《干旱地区农业研究》2023,(4):141-150

于2017—2018年和2018—2019年,在西北中部旱作雨养农业区以冬小麦‘康庄974’为试验材料,设秸秆带状覆盖(SM)、地膜覆盖(PM)和无覆盖对照(CK)共3个栽培处理,分析不同覆盖方式对小麦灌浆期土壤水分和温度及抗氧化能力的影响,探讨花后干物质积累量和粒重形成的关系。结果表明,随生育期推进,花后旗叶相对含水量(RWC)逐渐降低,丙二醛(MDA)含量逐渐升高,且PM较SM降、升幅度明显。与CK相比,覆盖显著提高花后旗叶抗氧化酶活性,增加渗透调节物质含量;开花时间越长,SM抗氧化酶活性升高的幅度越大,而PM主要提高花后7 d旗叶抗氧化能力。SM和PM的粒重分别较CK增加14.3%和19.1%(P<0.05)。相关分析表明,土壤水分(SW)是影响旗叶生理活性的关键因子,提高SW,有利于增加RWC(r=0.84^**),从而提高旗叶抗氧化能力,其中RWC和抗坏血酸酶(ASA)活性呈极显著正相关关系(r=0.82^**);ASA活性和脯氨酸(Pro)含量呈极显著正相关关系(r=0.94^**),和MDA含量呈极显著负相...  相似文献   

氮磷钾配施对红花产量及其有效成分在器官中分配的影响     

胡喜巧  陈红芝  邬佩宏  路瑶瑶  周保英  杨文平  孟丽 《干旱地区农业研究》2023,(1):53-62

于2018—2019年在河南科技学院试验田,以百农红花1号为材料,设计氮、磷、钾三因素和无肥(N0、P0、K0)、低肥(N1、P1、K1)、中肥(N2、P2、K2)、高肥(N3、P3、K3)四水平试验,以T1(N0P0K0)为对照,T2 (N0P2K2)、T3(N1P2K2)、T4(N2P2K2)、T5(N3P2K2)、T6(N2P0K2)、T7(N2P1K2)、T8(N2P3K2)、T9(N2P2K0)、T10(N2P2K1)、T11(N2P2K3)为不同配施水平,共11个处理,研究氮、磷、钾肥用量及配比对红花产量的影响,探讨氮、磷、钾不同用量及配比下红花生育期各器官中有效成分分配规律。结果表明：红花花产量随氮肥用量的增加而增加,以T5最高,与T1相比增加59.44%,随磷肥和钾肥用量的增加呈先上升后下降趋势,均以T4最大,与T1相比增加42.87%;红花种子产量随氮、磷、钾肥用量的增加呈先上升后下降的趋势,均以T4最高,与T1相比增加116.52%。氮、磷、钾肥不同用量及配比对红花生育期各器官中黄酮含量影响有相同规律,从苗期到成熟期,叶和茎黄酮含量均表现为先下降后上升的趋势,根中黄...  相似文献   

Flavonoid production in tomato mediates both direct and indirect plant defences against whiteflies in tritrophic interactions     

Fengbo Yang  Haowei Shen  Tianyu Huang  Qixi Yao  Jinyu Hu  Juan Tang  Rong Zhang  Hong Tong  Qingjun Wu  Youjun Zhang  Qi Su 《Pest management science》2023,79(11):4644-4654

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反转录-重组酶介导等温扩增（RT-RAA）快速检测基因2型猪繁殖与呼吸综合征病毒     

陈耀  仲欣雨  吴陈雨  丁雪  黄璟  王欢莉  范忠军  孙静  陈星逸  余树培  夏文龙 《中国动物检疫》2023,40(1):121-127

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双向电液比例张力绞车的直接自适应鲁棒控制     

钟天宇  王庆丰  姚斌 《农业机械学报》2007,38(11):144-148

设计了基于比例溢流阀调压回路的双向电液比例张力绞车,并进行了理论分析。针对液压系统的不确定性因素以及卷绕机构参数变化对张力控制的影响,提出了直接自适应鲁棒电液张力控制方法,并用张力负载模拟试验系统进行了研究。理论分析与试验均表明,对于具有不确定性因素的张力跟踪控制,所设计的电液张力绞车及其控制策略,具有良好的控制性能。  相似文献   

弱光条件下桃光合作用的动态变化及品种差异分析   总被引：3，自引：1，他引：3  

于桉  孔云  姚允聪 《中国农学通报》2005,21(9):242-248,254

就日光温室桃树光合生理进行了详尽的研究,结果表明:1d中叶温与气温以14:00时较高。遮阴明显地降低了到达叶片表面的有效光合辐射、叶温与气温。桃叶片的光合速率日变化为双峰曲线,峰值大约出现在上午10:00与下午14:00时;不同品种弱光下的光合速率有明显的差异,以NJN较高,其次为五月火与麦香。遮阴降低了桃叶片的光合速率,以NJN光合速率降低的较少,说明NJN能适应设施栽培。气孔阻力与蒸腾速率则随着遮阴程度的增加而增加。桃中位叶的光合速率较幼叶高。  相似文献   

Studies on the Oxidation of Weathered Coal with Nitric Acid     

Liang Renjie  Yao Shusen  Zhang Shilin  Hu Daiying  Wang Nanmu 《保鲜与加工》1996,(3):79-83

A raseach has been done on the possibility of producing nitriochumic acid fromweathered coal of Nantong,Sichuan,oxidised by nithic acid.The optimun reacting conditions,whichare found through the way of orthogonal experiment,are suggested as follows:concentration ofHON3 35%,rate of HON3 to coal 3.5,reacting take 15 min,and reaction temperature 369 K. Thecomposition of product is Wf 2. 91 %;Ag 14.06%;HAg 78.28%;Ng 1.62%.  相似文献   

Synthetic Assessment of Power Transformer Condition Based on Matter-element Theory     

XlONG Hao  SUN Cai-xin  DU Peng  DAI Yao  WANG Qian 《保鲜与加工》2006,(10):24-28

Condition assessment on power transformer is an important approach to improve reliability of transformer and provide proper decision-making for condition-based maintenance. For long, static assessment method used in the past has already not adapted to the development of modem electricity transmission and distribution technology. So based on the deficiency of fuzzy synthetic assessment method, matter-element synthetic assessment method is introduced. The method can evaluate transformer condition not only in quality but also in quantity. Because each index weight can be given fairly and fast through improved analytic hierarchy process, which need not carry on consistency chec accuracy of assessment can be improved. Examples analysis has proved that assessment method of power transformer condition based on matter-element theory is rational and effective.  相似文献