Electron microscope study of chicken proventriculus     

I Horváth 《Acta veterinaria Academiae Scientiarum Hungaricae》1974,24(1):85-97

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Translation of the edited mRNA for cytochrome b in trypanosome mitochondria     

Horváth A  Berry EA  Maslov DA 《Science (New York, N.Y.)》2000,287(5458):1639-1640

The type of RNA editing found in the kinetoplast-mitochondria of trypanosomes and related protozoa, involving uridylate insertions and deletions, creates translatable messenger RNAs (mRNAs) out of nonsense pre-edited RNAs by correcting encoded defects that vary from simple frameshifts to large "cryptic" regions. However, any evidence for translation of these mRNAs in the kinetoplast has been missing for decades. We identified a kinetoplast-encoded protein, apocytochrome b, whose mRNA is edited in the 5' region. The determined amino-terminal sequence of the protein coincides with the predicted sequence derived from the edited region, demonstrating that the cognate apocytochrome b mRNA is translated into a functional protein. This finding represents the first direct evidence for a functional translation system in the kinetoplasts.  相似文献   

Red cell carbonic anhydrase polymorphism of buffalo (Bubalus bubalis L.)     

P Soos  I Horváth  P Sólyom 《Acta veterinaria Academiae Scientiarum Hungaricae》1971,21(4):413-415

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Isolation and identification of Vibrio coli and Borrelia like germs in the pig's dysentery     

H Krizanová  M Beseda  Z Koppel  M Halasa  J Horváth 《Veterinární medicína》1972,17(9):527-538

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Lifestyle,habitat and farmers' risk of exposure to tick bites in an endemic area of tick‐borne diseases in Hungary          下载免费PDF全文

S. Li  L. Juhász‐Horváth  A. Trájer  L. Pintér  M. D. A. Rounsevell  P. A. Harrison 《Zoonoses and public health》2018,65(1):e248-e253

Controlling tick bites on farmers is important to the management of tick‐borne diseases and occupational health risks in agriculture. Based on an extensive household survey conducted between June and August 2015 with 219 farmers from western Hungary where tick‐borne diseases are endemic, we analysed the pattern of farmers' self‐reported contacts with ticks and investigated the potential interactions between farmers, landscape and the risk of exposure to tick bites. We developed a lifestyle typology based on farmers' socioeconomic profiles, farming objectives and time use patterns, and a habitat typology describing different configurations of tick habitats and agricultural areas in place of farming. We found no relationship between tick exposure risk and self‐prevention. The lifestyle typology could be used to classify the risk of tick bites and the adoption of prevention measures into different levels, the difference between which could further be modified by the habitat typology. Our results suggest that (i) farmers who are frequently engaged in outdoor recreations and (ii) part‐time and inexperienced farmers who have lower rate of preventive actions are likely to experience greater exposure to tick bites either in less cultivated, semi‐natural habitats or in agricultural landscape with highly diverse land uses. Future disease prevention practices should take into consideration the interaction of lifestyle and habitat and the need to associate different farmer groups with different landscape configurations.  相似文献   

Fertilizing capacity and motility of tench Tinca tinca (L., 1758) sperm following cryopreservation          下载免费PDF全文

Jelena Lujić  Gergely Bernáth  Zoran Marinović  Nataša Radojković  Vladica Simić  Miroslav Ćirković  Béla Urbányi  Ákos Horváth 《Aquaculture Research》2017,48(1):102-110

Experiments were carried out to develop an optimal cryopreservation protocol for tench sperm by testing the fertilizing capacity and motility parameters including progressive motility, curvilinear velocity (VCL) and linearity (LIN) of cryopreserved sperm. Three experiments were designed to this aim: first experiment where we tested the effects of two extenders (sugar‐based Grayling and ion‐based Kurokura 180) and two cryoprotectants (DMSO and methanol) on fertilization and hatching success; second where we tested the effect of cryoprotectant type (methanol or DMSO) in different concentrations (5%, 10% and 15%) on fertilization and hatching success; and third where we tested the effect of two cryoprotectants (methanol and DMSO) on sperm motility parameters (progressive motility, VCL and LIN) after 4 h post‐thaw storage (4°C). Sperm prepared with the sugar‐based Grayling extender displayed better fertilization and hatching rates independently of the applied cryoprotectant most likely due to glucose present which acted as an external cryoprotectant. Concerning cryoprotectant concentrations, the use of 10% methanol yielded the highest fertilization (85 ± 15%) and hatching (80 ± 13%) rates, which were significantly higher than in all other groups. During the post‐thaw storage time, 5% methanol, 10% methanol and 5% DMSO groups had significantly higher motility parameters than other groups and we observed no significant decline in any of the parameters during the storage time. Overall, we found that a sugar‐based extender in combination with methanol as cryoprotectant is suitable for the cryopreservation of tench sperm and allows storage of cryopreserved sperm for up to 4 h post thaw.  相似文献   

Commercial‐scale cryopreservation of wels catfish (Silurus glanis) semen     

Zoltán Bokor  Béla Urbányi  László Horváth  Ákos Horváth 《Aquaculture Research》2010,41(10):1549-1551

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Stress preconditioning of boar spermatozoa: a new approach to enhance semen quality     

Pribenszky C  Horváth A  Végh L  Huang SY  Kuo YH  Szenci O 《Reproduction in domestic animals》2011,46(Z2):26-30

Semen preparation and cryopreservation require finely adjusted procedures. Gametes are sensitive to environmental stresses, so in vitro procedures aim to minimize the inevitable harmful conditions. Applying stress to precondition cells has only been investigated recently. Studies demonstrated that by utilizing a well defined and properly applied hydrostatic pressure (HP) stress treatment to spermatozoa before in vitro storage, cryopreservation or insemination, cell survival and fertility improved compared with untreated controls. The birth of healthy piglets from treated fresh or frozen-thawed semen demonstrates the in vivo safety of the procedure. Although the biological mechanism is still unclear, several processes incorporating cellular stress response might explain the observations. This paper summarizes results, background, aspects and considerations of HP treatment for porcine semen. The new principle, i.e. to improve the stress tolerance by a defined sublethal stress may outline a new strategy in assisted reproductive technologies with unique theoretical and practical consequences.  相似文献   

Outbreak of Klebsiella oxytoca enterocolitis on a rabbit farm in Hungary     

Német Z  Szenci O  Horváth A  Makrai L  Kis T  Tóth B  Biksi I 《The Veterinary record》2011,168(9):243

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Genetic variation of melon (C. melo) compared to an extinct landrace from the Middle Ages (Hungary) I. rDNA, SSR and SNP analysis of 47 cultivars     

Z. Szabó  G. Gyulai  M. Humphreys  L. Horváth  A. Bittsánszky  R. Lágler  L. Heszky 《Euphytica》2005,146(1-2):87-94

Summary Microsatellite profiles of 47 melon cultivars and landraces were analyzed and compared to the aDNA (ancient DNA) of seed remains from an extinct sample recovered from the 15th century (Budapest, Hungary). An aseptic incubation followed by ITS (internal transcribed spacer) analysis was used to exclude the exogenously and endogenously contaminated medieval seeds and to detect SNPs (single nucleotide polymorphism) in ITS1-5.8S-ITS2 region of rDNA (ribosomal DNA). SNPs were observed at the 94–95 bp (GC to either RC, RS or AG) of ITS1; and at 414 bp (A-to-T substitution), 470 bp (T to Y or C), 610 bp (A to R or G) and 633 bp (A-to-G transition) of ITS2. For comparative microsatellite analysis SSRs (simple sequence repeats) detected by ALF (automated laser fluorometer) was used. Eight of the 20 SSR primer pairs amplified 40 microsatellite alleles in identical fragment ranges. A total of 485 alleles were detected in the 47 melon cultivars. The number of alleles per marker ranged from 2 to 7 with an average of 5.7 including CMCT44 (2 alleles), CMAG59 (5 alleles), CMGA104 (5 alleles), CMCT134 (4 alleles), CMTA134 (6 alleles), CMCTT144 (7 alleles), CMTC168 (6 alleles) and CMCT170 (5 alleles). Sequence analysis of the microsatellite alleles showed different fragment lengths depending on changes in the number of unit of core sequences. Dendrogram produced by SPSS11 based on the presence versus absence of SSR alleles revealed that medieval melon had the closest genetic similarity to a registered melon cultivar Hógolyó selected from an old Hungarian melon landrace. These results also indicated that cloned DNA sequences recovered from aDNA of medieval melon can be of use for molecular breeding of modern melon cultivars via gene transfer.  相似文献