斑点免疫吸附试验诊断羊脑多头蚴病的研究     

姚新华  周维等 《吉林畜牧兽医》2001,(12):3-5

以原头节可溶性粗抗原经Sephadex G-200层析纯化抗原为包被抗原，兔抗羊IgG-HRP结合物为显色剂，建立检测羊脑多头蚴病血清抗体的Dot-ELISA，并以ELISA作平行对照。结果，粗抗原和层析纯化抗原检测86头份羊脑多头蚴病阳性血清，其敏感性分别为94．18％和93．02％，两种抗原的敏感性无显著差异；检测122头份绦虫蚴病阴性血清，18头份棘球蚴病阳性血清，35头份细颈囊尾蚴病阳性血清，其特异性分别为90．29％和95．43％。两种抗原的特异性差异显著。Dot-ELISA和ELISA两种方法的符合率为100％。层析纯化抗原比粗抗原的特异性有了明显提高，而敏感性没有降低。层析纯化抗原和操作术式都具有良好的重复性，Dot-ELISA和ELISA对比试验结果相近，且具简便、快速及不需要复杂设备等优点，是一种检测羊脑多头蚴病血清抗体的理想方法。  相似文献   

Studies on efficacy and stability of a vaccine bait containing ERA strain of rabies virus propagated in a BHK-21 cell line.          下载免费PDF全文

K F Lawson  H Chiu  M Matson  P Bachmann    J B Campbell 《Canadian journal of veterinary research》1992,56(2):135-141

In a dose response study in foxes, the median protective dose of ERA BHK21 vaccine in a blister pack bait was 10(6.0) tissue culture infective doses (TCID)/mL, while artificially aged baits with titers of 10(6.3) TCID/mL induced seroconversion in 78% of foxes. There was no significant difference in the development of antibodies in foxes receiving 1, 2 or 3 mL volumes of vaccine in the bait. When baits were exposed to the elements and fed to foxes over a 21 day period, 85% of the animals seroconverted. Age, sex and the way in which the vaccine container was contacted did not appear to be factors in the responses of these animals. Juvenile foxes, approximately six months of age, were marked more readily with the tetracycline bait marker than older animals. Approximately 25% of foxes did not appear to respond well to vaccination and the titer of the vaccine was a critical factor in producing seroconversion in these animals.  相似文献   

Inheritance of myotonic discharges in American quarter horses and the relationship to hyperkalemic periodic paralysis.          下载免费PDF全文

J M Naylor  J A Robinson  E C Crichlow    J E Steiss 《Canadian journal of veterinary research》1992,56(1):62-66

Electromyography (EMG) was used to detect myotonic discharges in Quarter Horse breeding stock and to follow the results of mating horses with hyperkalemic periodic paralysis (HPP). The studies were performed on two brood mare farms. A total of six breeding stock showed myotonic discharges and 15 were nonmyotonic. Myotonic discharges were seen in five of six horses belonging to the blood line previously implicated as being predisposed to HPP. Two of these horses had shown clinical signs of HPP. Only one of 15 breeding horses unrelated to the HPP predisposed blood line showed myotonic discharges. When both parents were non-myotonic on EMG than the F1 generation (n = 6) were also nonmyotonic. When a stallion with HPP and myotonic discharges was mated to eight nonmyotonic mares over a six year period half the animals of the F1 generation (n = 25) showed myotonic discharges. When both parents showed myotonic discharges four F1 offspring were myotonic and two were nonmyotonic on EMG testing. There was no evidence of sex linkage. The results are consistent with an autosomal dominant mode of inheritance. Hyperkalemic periodic paralysis and myotonic discharges on EMG may be different manifestations of the same underlying defect.  相似文献   

Obituary: Dr. William J. Dorward          下载免费PDF全文

W.G.D. Yates  Peter Ide 《The Canadian veterinary journal. La revue veterinaire canadienne》1992,33(8):492

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剌槐种子萌发过程的微量热法研究     

胡云楚  周培疆 《种子》1994,(6):16-17,59

用微量热法测定了剌槐种子的萌发热谱，发现其产热规律与其吸水规律和呼吸规律相关，并获得了衡量种子活力４个参数。  相似文献   

Studies on mutlispecific resistance of gastrointestinal nematodes to benzimidazoles on dairy-goat farms     

J. Cabaret  H. M. Baudet  J. Devos  J. Hubert  J. Cortet  C. Sauv 《Veterinary parasitology》1995,60(3-4):331-337

Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm).  相似文献   

心脏体外循环系统检测植物外啡肽活性的研究     

孙镇平  华棣  吕立力  熊万斌  周业飞 《猪业科学》2003,20(7):71-72

本文利用猪胃蛋白酶在37℃条件下降解中筋粉,采集不同反应时间的酶解液样品,低温离心处理后,分别加入蛙心体外循环系统中,测定心肌收缩力变化,并随即加入阿片肽阻断剂-纳洛酮,检测植物外啡肽活性。结果表明,酶解液能够引起心肌收缩力加强,并可被纳洛酮逆转,证明酶解液具有植物外啡肽活性。  相似文献   

福建城乡差距与统筹发展探讨     

黄炳茂 《台湾农业探索》2006,(3):46-48

城乡二元经济结构的存在,导致我国城乡发展存在很大的差距。党的十六大提出实现全面建设小康社会的奋斗目标,其关键要统筹城乡经济发展,缩小城乡差别,消除二元经济结构,促进农民收入的提高。本文在分析福建城乡发展差距及其变化的基础上,提出统筹城乡发展的若干对策建议。  相似文献   

一种从桑蚕分离得到的抗菌肽moricin的溶液结构     

边见光石桥纯  原诚一山川稔 《广东蚕业》2003,37(2):49-49

是一种从桑蚕分离得到的新型抗菌肽，它由42个氨基酸组成。它非常基本，而且它的氨基酸序列与一些别的抗菌肽没有显的相似性。根据双向^1H—核磁共振分光数据分析得出moricin的甲醇溶液有20种结构。这些溶液结构显示了一种由长α—螺旋构成的独特结构。除了4个氨基末端残基和6个羧基末端残基之外，α—螺旋整条肽长包含八个转角。静电表层图显示了α—螺旋氨基末端5～22个残基部分是一种两亲性α—螺旋，其疏水性和亲水性界面有一个很明显的间隔；除了Asp30(天冬氨酸)位点是负电荷表层外，羧基末端23～36个残基部分是一种疏水性α—螺旋。结果表明，α—螺旋的两亲性的氨基末端片段的主要作用是增强膜的渗透性而杀死细菌。  相似文献   

胃肠道线虫的黏膜免疫     

韩周路义鑫  宋铭忻 《中国兽医科技》2006,36(8):679-683

从机体抗胃肠道线虫的黏膜免疫、胃肠道黏膜的抗原加工与递呈、胃肠道黏膜抵御寄生虫的效应机制等几方面论述了胃肠道线虫的免疫机理和免疫学研究的进展，旨在进一步阐明用免疫介入方法防治胃肠道线虫的理论基础，以解决胃肠道线虫治疗过程中的药物残留以及寄生虫的抗药性问题。  相似文献