Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells.     

H K Pradhan  G C Mohanty  W Y Lee  B Patnaik 《Veterinary immunology and immunopathology》1991,29(3-4):229-238

Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test.  相似文献   

杂种小麦产量和蛋白质含量的优势表现与优势环境分析(简报)     

刘录祥  黄铁城  张树榛  刘广田 《中国农业大学学报》1993,(4):107-108

许多研究表明,杂种小麦在产量和主要品质性状上存在显著的基因型与环境互作,杂种小麦比纯系品种更能适应变化的环境条件,表现出更好的稳定性。但是,关于杂种小麦优于纯系品种所表现出的杂种优势在不同环境中的表现则报道很少。正如一种性状的表现型由  相似文献   

Injection and sampling methods for drug residue study in calf muscle     

Y Takahashi  M Iida  Y Nishida  Y Kido 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(5):995-1001

Eight calves, weighing 50-150 kg, were given intramuscularly 5 ml of ampicillin (ABPC) aqueous suspensions (200 mg potency/ml) in their right and left gluteal and femoral regions. All calves were sacrificed one hour later to confirm the location of injected drug. The drug was found in a muscle layer when injected with a needle 15 mm long to the following positions, 1. the midpoint between the central position of the gluteal region (CG) and the tuber coxae (M-CTc), 2. the midpoint between CG and the tuber ossis ischii (M-CTo), 3. the central position of M. semimembranaceus in the femoral region (CF). Seven calves, weighing 130-150 kg, were given intramuscularly 5 ml of ABPC suspensions at M-CTo and CF and sacrificed one hour (4 calves) and 3 days (3 calves) later. ABPC diffused along the long axis of the muscle fibers but not to the radial direction. ABPC was detected only in the injected muscle layer even after 3 days indicating that the drug did not diffuse to the neighboring muscles. In the injected muscle layer, concentration of ABPC was remarkably different from part to part. From these results, sampling of the injected muscle for the drug residue study was proposed as follows: 1. isolate about 100 g of muscle just under the stick point marked on the skin considering the direction of drug diffusion, and 2. isolate separately about 200 g of the surrounding muscle to confirm if the sampling is appropriate.  相似文献   

Cellular injury and lipid peroxidation induced by hexavalent chromium in isolated rat hepatocytes   总被引：1，自引：0，他引：1  

S Ueno  N Susa  Y Furukawa  K Aikawa  I Itagaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(1):137-145

In order to elucidate the relationship between cellular injury and lipid peroxidation induced by hexavalent chromium (CrVI), isolated rat hepatocytes treated with any one of scavengers of active oxygen species, antioxidants or antichromium agent were incubated with K2Cr2O7 as CrVI (1 mM Cr). After the incubation, the development of lipid peroxidation was determined as thiobarbituric acid (TBA)-reacting materials in total lipid extracts from the incubated hepatocytes. Cellular injury was observed as a leakage of lactate dehydrogenase (LDH) from hepatocytes into incubation medium. The contents of reduced glutathione (GSH) in hepatocytes were also assessed. Results obtained were as follows: (1) CrVI facilitated lipid peroxidation in isolated hepatocytes after 20 min of incubation. On the other hand, the cellular injury induced by CrVI was barely observed even after 60 min of incubation. (2) The CrVI-induced lipid peroxidation was inhibited by catalase and mannitol as scavengers of active oxygen species, or N,N'-diphenyl-p-phenylenediamine and alpha-tocopherol as antioxidants. However the cytotoxicity of CrVI could not be prevented by these chemicals. (3) CrVI depleted the contents of intracellular GSH and diminished the activities of glutathione reductase (GR) and glutathione-S-transferase (GST) except glutathione peroxidase. (4) The scavengers of active oxygen species and the antioxidants could not prevent the depletion of intracellular GSH induced by CrVI. (6) Ascorbic acid, antichromium agent, prevented all of the lipid peroxidation, the cellular injury and intracellular GSH depletion induced by CrVI.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Distribution of Cytochrome P450-side Chain Cleavage in the Theca Interna Layers of Bovine Small Antral and Cystic Follicles   总被引：1，自引：0，他引：1  

N Isobe  T Nakao  Y Yoshimura 《Reproduction in domestic animals》2003,38(5):405-409

Cystic follicle is anovulatory follicular structure that is caused by an endocrine imbalance. The activity of cytochrome P450‐side chain cleavage (P450scc) is essential for the initiation of steroidogenesis in the follicle. The present study was designed to compare the frequency of cells containing P450scc between healthy and atretic small antral follicles, and among several types (I, II and III, classified based on the presence of granulosa layer) of cystic follicles. Paraffin sections of healthy (2–5 mm in diameter), atretic (2–5 mm) and cystic follicles (>25 mm) were immunohistochemically stained with rabbit polyclonal antibody to bovine P450scc. The P450scc‐positive cells were counted in four different regions of the follicles from the apical to the basal side. In small antral follicles and cystic follicles, P450scc‐positive cells were localized in the theca interna layers but not granulosa layers. The P450scc‐positive cell populations decreased in the late atretic follicles compared with the early and advanced atretic follicles at all the regions of follicle. Type III cystic follicles showed significantly lower frequencies of P450scc‐positive cells than those in the types I and II cystic follicles. These results suggest that in both small and cystic follicles in cows, total loss of granulosa cells may be associated with the reduction of frequency of P450scc‐positive cells in theca interna layer.  相似文献   

转抗菌肽基因酵母安全评价及在饲料中应用   总被引：1，自引：0，他引：1  

黄自然廖富蘋  黄国庆 《广东蚕业》2003,37(1):34-37

1945年弗莱明(Fleming)发现青霉素,1952年瓦克斯曼(Waksman)发现链霉素,先后获得诺贝尔奖。抗生素的发现造福人类作出很大贡献。近来,由于滥用抗生素而诱发耐药性病原菌的产生,造成医疗上的困难,又由于抗生素的毒副作用及残留问题,日益引起社会的重视。1998年欧共体禁止抗生素用作畜禽饲料添加剂,  相似文献   

THE TREATMENT OF EXTREME CASES OF "SNIFFLES" IN THE RAT WITH SULFAPYRIDINE     

Billingslea FY 《Science (New York, N.Y.)》1940,91(2349):19

  相似文献   

Intra‐uterine Infection with Babesia bovis in a 2‐day‐old Calf     

I. Yeruham  Y. Avidar  I. Aroch  A. Hadani 《Zoonoses and public health》2003,50(2):60-62

Infection with Babesia bovis was diagnosed in a 2‐day‐old female calf apparently transmitted in utero. The calf was born as the second calving to a cross‐bred beef cow permanently on pasture. Diagnosis was based upon identification of B. bovis in peripheral blood smears and clinical signs which included fever, jaundice, pale mucous membranes and convulsions. Anaemia, leucocytosis, thrombocytopenia and lymphocytosis were noted at the febrile acute stage of the disease. The blood smears revealed evidence of regeneration of toxic neutrophils with a left shift, severe spherocytosis and high degree of basophilic stippling. Elevated concentration of aspartate aminotransferase, lactate dehydrogenase, and creatine kinase were also noted, and were probably the result of haemolysis, dehydration and muscle damage because of recumbancy. Elevated total bilirubin concentration following haemolysis resulted in jaundice. The neurological symptoms observed were probably caused by sludging of parasitized erythrocytes in the brain capillaries. The calf recovered following treatment with diminazene aceturate and the recovery was followed up clinically, haematologically and biochemically.  相似文献   

加强监督执法工作 控制动物产品残留     

邓勇  黄勇 《中国动物检疫》2003,20(1):16-16

随着人们生活水平的不断提高 ,动物源性食品安全越来越引起人们的重视。要真正保证人们吃上放心的动物源性食品 ,保证人们吃上“放心肉” ,在动物产品进入“菜蓝子”之前只检出传染病和寄生虫病 ,是远远不够的。目前动物产品农药、药物、重金属等残留超标问题已严重影响人民群众身体健康 ,多起有毒有害物质残留中毒事件使人们谈肉色变 ,有的残留还在人体中富积 ,对人体造成慢性毒害 ,引起过敏、致畸、致癌 ,影响儿童生长发育。要控制动物产品残留 ,必须严格控制饲料、兽药的生产、经营及使用 ,严格控制动物饲养、屠宰、加工等多个环节。《饲…  相似文献   

干番茄渣在蛋鸡饲料中的应用     

黄彪  戴斌彬 《饲料广角》2009,(15):41-43

本文旨在研究用干番茄渣（DTP）部分或全部替代大豆、玉米、小麦、麦麸饲料时，对蛋鸡生产性能、蛋品质的影响。将288只试验鸡（27—38周龄）随机分成4组，每组72只．分别为：DTP0（对照组）、DTP50、DTP100、DTP150（每吨饲料中分别含0、50、100、150kg DTP）。与对照组相比．DTP100组使其产蛋量、产蛋总重分别增加2．7％、4．1％．而鸡的体重、蛋重、日消耗饲料量、蛋壳重、蛋壳厚、哈夫单位、蛋黄颜色均与对照组相似。DTP150组使产蛋量、产蛋总重分别降低3．6％、3．0％，但饲料转化率增加2．9％。结果表明．DTP可以作为蛋鸡的饲料原料。  相似文献