Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats     

XU Rui  ZHANG Yun  ZHANG Mei  GE Zhi-ming  CHEN Wen-qiang  LI Xiu-chang  CAI Heng  ZHU Hui 《园艺学报》2003,19(5):642-644

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.  相似文献   

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats     

CHEN Hu  CAI Ding-fang  LIANG Hui  CHENG Jie-shi 《园艺学报》2003,19(7):917-919

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.  相似文献   

姬松茸与双孢蘑菇不同菌株的RAPD扩增研究   总被引：6，自引：1，他引：6  

李三暑  林新坚  陈惠成  雷锦桂  林勇  江枝和 《食用菌学报》2002,9(3):1-4

以4个姬松茸菌株和2个双孢蘑菇菌株为材料，用20个随机引物对它们进行RAPD扩增，通过对供试菌株遗传相似系数的估算和系统聚类分析，构建姬松茸和双孢蘑菇遗传相关树状聚类图谱，结果表明，进行RAPD扩增的20个随机引物中，有12个能产生三种带型，当遗传相似系数升至0.8713时，这6个菌株被分为3类，第一类为菌株18和26，第二类为菌株13和12，第三类为菌株33和45。  相似文献   

莴苣‘红帆’品种下胚轴愈伤组织诱导与植株再生   总被引：2，自引：0，他引：2  

高辉  苟晓松  邓运涛  李旭锋 《园艺学报》2002,29(5):486-489

 莴苣下胚轴愈伤组织诱导时, MS+ 6-BA 0. 5 mg/ L+ 2, 4-D 1 mg/ L+ NAA 0. 1 mg/ L 效果最好,出愈率达94%; 愈伤组织分化为芽时, MS+ 6-BA 1. 0 mg / L+ NAA 0. 05 mg/ L 分化频率最高, 达100% ; 再生苗在1/ 2MS+ NAA 0. 5 mg/ L 中诱导生根成为完整植株。  相似文献   

Expression of IL-6 mRNA in endometrium with endometriosis     

ZHENG Hui  LI Hong-yi  WANG Zi-neng  ZHAO Ying-she  YU Li  HE Si-cun  BAI Zhi-quan  ZHOU Zuo-yan  YAO Ping  WANG Yue-chun 《园艺学报》2002,18(5):553-555

AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.  相似文献   

Change of central noradrenergic system in Alzheimer's disease     

WANG Yao-chun  WANG Zi-dong  HE Si-chun  ZHENG Hui  ZHOU Li-li 《园艺学报》2002,18(9):1162-1164

The abnormality of the central noradrenergic system in Alzheimer's disease has two cases: decrease or increase. The former is easy to be understood, because it is resulted from the outstanding forfeit of the noradrenergic neurons in the nucleus locus ceruleus (nLC); but the noradrenalin concentration does not change and enen increase, it seems to disagree with the loss of the noradrenergic neurons in the nLC. This article put emphasis on it, enumerated the related evidence and analyzed the possible causes. At present time, the drugs to increase noradrenalin is used mostly in clinic. This paper summarized it and put forth our own opinions.  相似文献   

利用RAPD标记评价桃种间杂交一代群体的分离方式   总被引：6，自引：2，他引：6  

乔飞  王力荣  范崇辉  朱更瑞  方伟超 《果树学报》2003,20(4):310-312

以毛桃2-7(Prunus persica)、山桃白山碧桃(Prunus davidiana)以及两者的F_1代为试材,对RAPD标记的分离方式进行分析。结果表明,RAPD标记在F_1代呈3种分离方式:孟德尔分离、偏离孟德尔分离规律、异常分离。3种分离位点出现的频率和数量分别为:80%、240,14%、42,6%、18。呈孟德尔分离的情况有:不分离、1:1分离和3:1分离,其中不分离的频率为64％。并对偏离孟德尔分离比例和异常分离的RAPD标记进行分析。其研究结果可为该群体是否适合构建遗传连锁图谱进行评价及进一步利用该群体奠定良好基础。  相似文献   

灰葡萄孢菌nit突变体的诱导及其在营养体亲和性测定中的应用初探   总被引：5，自引：1，他引：5  

高俊明  刘慧平  王建明  李新凤  韩巨才 《植物病理学报》2003,33(3):233-236

 从山西运城、临汾、长治、晋中、大同等地保护地黄瓜灰霉病病株上采集、分离的分属于3个不同菌丝融合群的8个灰葡萄孢菌单孢菌株,经氯酸盐诱导处理,共获得了抗氯酸盐的硝酸盐利用缺陷突变体(nit突变体)59株,其中nit1型38株,nit3型10株,nitM型11株。所有nit突变株分别在PDA斜面转管培养3次(21 d)后,除6株恢复成野生菌株外,其余多数nit突变菌株表现稳定。来源于同一野生菌株的不同类型nit突变体间或同一菌丝融合群不同野生菌株的nit突变体间可产生互补反应而形成异核体,其中以nitM型突变株互补性最好,在利用nit突变体测定灰葡萄孢菌营养体亲和性时应作为标准菌株。来源于不同菌丝融合群的nit突变体间不能产生互补反应。  相似文献   

阿魏蘑与杏鲍菇高产优质栽培模式研究   总被引：2，自引：0，他引：2  

万鲁长  张柏松  黄春燕  郭惠东  单洪涛  李斌  姜春君  陈黎明  姚建国 《食用菌学报》2003,10(2):40-44

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。  相似文献   

注射用盐酸土霉素的细菌内毒素检查     

赵晖  孙玉梅  吴好庭 《中国兽药杂志》2004,38(11):17-18,40

参照<中国兽药典>二○○○年版一部附录细菌内毒素检查法,研究了注射用盐酸土霉素对细菌内毒素检测的干扰情况,确定0.312 5 mg/mL浓度的供试品溶液对检测无干扰作用,建立了注射用盐酸土霉素的细菌内毒素限量检查方法.  相似文献