EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

茶树蛋白激酶基因CsCIPK的克隆与表达特性分析     

刘昊  王文丽  滕瑞敏  李辉  王瑜  王永鑫  庄静 《园艺学报》2019,46(1):96

以茶树品种‘龙井43’作为材料,利用RT-PCR方法,从茶树的cDNA中克隆得到1个编码蛋白激酶的基因,命名为CsCIPK。序列分析表明,CsCIPK开放阅读框长度为1 341 bp,编码446个氨基酸,蛋白质分子量为414234。蛋白功能域预测和多重对比显示,CsCIPK蛋白含有1个保守的N端激酶结构域和1个相对不保守的C端调节结构域,即丝氨酸/苏氨酸激酶结构域和NAF结构域。理化性质、亲/疏水性、无序化分析显示,CsCIPK属于疏水性蛋白,理论等电点为7.04,有4段无序化区域,其二级结构分析显示主要由α螺旋、不规则卷曲组成。通过实时荧光定量PCR对‘龙井43’和‘安吉白茶’中的CsCIPK表达特性进行分析。结果显示‘龙井43’中CsCIPK的相对表达量在高温、干旱及盐处理4 h、低温处理24 h时达到最高。‘安吉白茶’中CsCIPK的相对表达量在高温及盐处理4 h、低温及干旱处理1h时达到最高。CsCIPK在‘龙井43’的根中,‘安吉白茶’茎中表达量最高。不同浓度的GA和IBA处理‘龙井43’茶苗,结果显示0.2 mmol·L-1 GA处理后,CsCIPK表达量先升高后下降,6 d时处理组为对照组的62倍;0.6 mmol·L-1 IBA处理后,CsCIPK的表达量在3 d时显著高于对照组;不同浓度GA和IBA处理后,9 d时CsCIPK表达量均显著低于对照。  相似文献   

Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp. chinensis Makino     

Peirong Li  Tongbing Su  Huiping Wang  Xiuyun Zhao  Weihong Wang  Yangjun Yu  Deshuang Zhang  Changlong Wen  Shuancang Yu  Fenglan Zhang 《Plant Breeding》2019,138(3):309-324

Single‐nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non‐heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non‐heading Chinese cabbage inbred lines using a Kompetitive Allele Specific PCR (KASP) genotyping assay. On the basis of identified polymorphisms and the results of a principal component analysis, we selected 50 core SNPs that were balanced sufficiently to provide adequate information for genetic identification. The core SNPs were used for construction of a neighbour‐joining dendrogram that separated the 70 inbred lines into four main groups and several subgroups corresponding to Caixin, Heiyebaicai, Huangxinwu, Naibaicai, Taitsai, Pak‐choi, and Wutatsai. This categorization was superior to that achieved using a dataset of 479 polymorphic SNPs. To confirm the utility of the core SNP markers in genetic identification, we tested their stability and resolution using 162 commercial hybrid cultivars. The SNPs, which represent a cost‐effective, accurate marker set for germplasm analysis and cultivar identification, are suitable for molecular marker‐assisted breeding in non‐heading Chinese cabbage.  相似文献   

Effect of nitrogen root zone fertilization on rice yield,uptake and utilization of macronutrient in lower reaches of Yangtze River,China   总被引：1，自引：0，他引：1  

Xiaowei Liu  Huoyan Wang  Jianmin Zhou  Zhaoming Chen  Dianjun Lu  Dejin Zhu  Pingliang Deng 《Paddy and Water Environment》2017,15(3):625-638

Improper application of nitrogen (N) has led to high N losses and low N use efficiency in the lower reaches of Yangtze River in China. An effective method to solve such problems is the deep fertilized N in root zone (RZF). Limited information is available on the effect of RZF on the uptake of macronutrients (N, P and K) and rice yield. Field experiments, conducted from 2014 to 2015, compared the farmer fertilizer practice (FFP, with 225 kg ha^?1 of N, split into three doses) and RZF using the same rate but placing N 5 cm away from rice roots in holes 10 cm deep (RZF10) or 5 cm deep (RZF5) as a single application. The highest mean yield (10.0 t ha^?1) was obtained in RZF10, which was 19.5% more than that in FFP. Root zone fertilization of urea (whether 10 cm deep or 5 cm deep) resulted in greater accumulation of N, P and K in stem, leaf sheaths, leaf blades and grains compared to that in FFP in sandy and in loam soils. The uptake of N, P and K was the highest in RZF10 (average at 176.7, 66.2 and 179.1 kg ha^?1, respectively), higher than that in FFP by 45.0, 17.0 and 22.6%, respectively. N apparent recovery efficiency was markedly higher in RZF10 (53.1%) than in FFP (27.5%). RZF10 significantly increased the N, P, K uptake compared with FFP under different N rates in both sandy and loam soils. These results suggest that the N, P and K input amount should be re-determined under RZF.  相似文献   

红蓝LED光照强度对茶树生长及生物化学成分的影响   总被引：1，自引：0，他引：1  

王加真  金星  熊云梅  付玉迪  陈莹莹 《分子植物育种》2020,(5):1656-1660

以‘黔湄601’一年生茶树苗为试材,光量比为1∶3的LED红蓝光作为光源,探讨不同光照强度对茶树幼苗生长特性的影响、光合色素及生物化学成分的变化趋势,探索茶树苗生长发育对光强的响应机制。采用LED精准调节光质和光强,设置50μmol·m-2·s-1、100μmol·m-2·s-1、150μmol·m-2·s-1、200μmol·m-2·s-14个处理。结果表明,不同光强处理15 d后,50μmol·m-2·s-1光照强度有利于茶树叶色素含量的增加及Ca/Cb值的降低;100μmol·m-2·s-1光照强度有利于茶树叶茶多酚含量的降低及氨基酸含量的增加,酚氨比值最低;150μmol·m-2·s-1光照强度对茶树叶重增加有促进作用;200μmol·m-2·s-1光照强度有利于茶树叶茶多酚含量的增加和氨基酸、光合色素含量的减少;酚氨比值最高,光合色素含量最低。红蓝LED光照强度过低、过高都不利于茶叶品质形成,综合考虑,100μmol·m-2·s-1光照强度最有利于茶叶功能成分的积累,是茶叶LED光源设施栽培的理想光照强度。本研究结果对于设施茶树种植具有重要的参考意义。  相似文献   

The microbial diversity and structure in peatland forest in Indonesia     

Bing Liu  Muhamed Jamal Hosen Talukder  Eeva Terhonen  Maija Lampela  Harry Vasander  Hui Sun  Fred Asiegbu 《Soil Use and Management》2020,36(1):123-138

The microbial community structure and function under forest in tropical peatlands are poorly understood. In this study, we investigated the microbial community structure and diversity in natural peat swamp forest soil, disturbed peat soil and mineral soil in Central Kalimantan, Indonesia, using 454 pyrosequencing. The results showed that the natural peat soil had the greatest fungal species richness (Chao1), which was significantly (p < .05) larger than that in the other two soils. Community structure of both fungi and bacteria in natural peat soil differed significantly from that in the disturbed peat soil (p = .039 and p = .045, respectively). Ascomycota (40.5%) was the most abundant phylum across the three soils followed by Basidiomycota (18.8%), Zygomycota (<0.1%) and Glomeromycota (<0.1%). The linear discriminant analysis with effect size (LEfSe) showed that Ascomycota (p < .05) and genus Gliocephalotrichum (p < .05) dominated in natural peat soil. Functionally, pathotrophs were more abundant in disturbed peat soil (p < .05). Proteobacteria (43.8%) were the most abundant phylum followed by Acidobacteria (32.6%), Actinobacteria (9.8%), Planctomycetes (1.7%). Methylocystis, Telmatospirillum, Syntrophobacter, Sorangium and Opitutus were the more abundant genera in disturbed peat soil, whereas Nevskia and Schlesneria were more abundant in mineral soil and natural peat soil, respectively. The natural peat forest soil supported a more diverse microbiology; however, the land use of such a soil can change its microbial community structure. The results provide evidence that the disturbance of tropical peat land could lead to the introduction and spread of a large number of fungal diseases  相似文献   

Identification,characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)     

Wen-wen LIU  Min XIN  Meng-ji CAO  Meng QIN  Hui LIU  Shou-qi ZHAO  Xi-feng WANG 《农业科学学报》2018,17(10):2281-2291

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA(dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus(HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region(UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame(ORF) that encodes a deduced 4 867 amino acids(aa) polyprotein with three domains: RdRP, Hel and UGT(UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization(FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.  相似文献   

苗后除草剂喷施时期对杂草防治及冬油菜产量和品质的影响   总被引：1，自引：0，他引：1  

王芙蓉  张建学  郭岷江  张亚宏  范提平  王亚宏  张岩  裴国平  雷建明 《作物杂志》2020,36(5):204-81

为了探究苗后除草剂不同施用时期对北方冬油菜田间杂草的防控效果及其对冬油菜生长、产量和品质的影响,以当地主栽甘蓝型冬油菜天油2266和白菜型冬油菜天油H614为试验材料,选用对冬油菜安全的苗后除草剂高效氟吡甲禾灵,设置4个喷施时期处理,于2018-2019年开展大田试验。结果表明,越冬后不同时期喷施苗后除草剂,甘蓝型冬油菜田间杂草总数较对照减少57.54%~92.56%,杂草总鲜重较对照降低75.40%~97.48%,第2时期（3月25日）处理产量较对照增加354.50kg/hm²;白菜型冬油菜田间杂草株防效和鲜重防效分别为55.91%~92.02%和37.17%~97.46%,产量较对照增加2.29%~16.77%。因此,在冬油菜大量返青但未封行前施用苗后除草剂,可显著防控田间杂草并增加冬油菜籽粒产量。  相似文献   

优质国审棉品种——ZHM19     

黄慧  贺云新  张志刚  蒋杰  吴佶膛  张兴平  梅正鼎 《中国棉花》2020,47(11):38-39

概述了ZHM19的选育过程、特征特性、产量、纤维品质和抗病性等特点及主要栽培技术措施。  相似文献   

棉花叶枝数与种植密度对单株结铃数的影响   总被引：1，自引：1，他引：0  

朱继杰  赵红霞  王士杰  李妙  王国印  黄瑞恒  张晓  万艳霞 《中国棉花》2017,44(1):13-16

为了探明棉花叶枝数和密度对单株结铃的影响规律，试验设置5种留叶枝方式：不留叶枝（CK）、留1叶枝、留2叶枝、留3叶枝、留全部叶枝；5个种植密度（万株·hm^－2）：2.25，3.00，3.75，4.50，5.25。研究结果表明：在同一密度条件下，随叶枝的增多，单株铃数增加；留1叶枝单株铃数较对照增加10%左右，留2、3叶枝单株铃数较对照增加15%左右，保留全部叶枝较对照增加25%左右。在同一叶枝处理下，随密度的增加，单株铃数减少；密度每增加7500株·hm^－2，5种留叶枝方式的单株结铃分别减少2.60个、3.04个、3.06个、3.44个和3.71个。总铃数随叶枝和密度的增加而增加，当总成铃为88.24万个·hm^－2时产量最高。  相似文献