基因芯片技术及其在农业上的应用   总被引：5，自引：1，他引：5  

徐景升  张木清  陈如凯 《福建农林大学学报(自然科学版)》2002,31(1):62-66

基因芯片技术是近年来在生命科学技术领域中迅速发展起来的一项高新技术 .本文综述了基因芯片的产生背景、概念、原理、分类和制作方法 ,并展望了其在农业上的应用前景  相似文献   

Amazon forests green-up during 2005 drought     

Saleska SR  Didan K  Huete AR  da Rocha HR 《Science (New York, N.Y.)》2007,318(5850):612

Coupled climate-carbon cycle models suggest that Amazon forests are vulnerable to both long- and short-term droughts, but satellite observations showed a large-scale photosynthetic green-up in intact evergreen forests of the Amazon in response to a short, intense drought in 2005. These findings suggest that Amazon forests, although threatened by human-caused deforestation and fire and possibly by more severe long-term droughts, may be more resilient to climate changes than ecosystem models assume.  相似文献   

邻苯二甲酰亚胺乙氧基环甘油硫代磷脂缀合物的合成     

许新华  张松林  陈茹玉 《湖南农业大学学报(自然科学版)》2002,29(1)

以碘活化的六乙基亚磷酰三胺为磷酰化及环化试剂 ,经“一锅法”合成了邻苯二甲酰亚胺乙氧基环甘油硫代磷脂缀合物 ,收率达 90 %左右  相似文献   

Role of histone H3 lysine 27 methylation in X inactivation   总被引：1，自引：0，他引：1  

Plath K  Fang J  Mlynarczyk-Evans SK  Cao R  Worringer KA  Wang H  de la Cruz CC  Otte AP  Panning B  Zhang Y 《Science (New York, N.Y.)》2003,300(5616):131-135

The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation in extraembryonic cells but not of random X inactivation in embryonic cells. The Drosophila homolog of the Eed-Ezh2 PcG protein complex achieves gene silencing through methylation of histone H3 on lysine 27 (H3-K27), which suggests a role for H3-K27 methylation in imprinted X inactivation. Here we demonstrate that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation. Recruitment of the complex and methylation on the Xi depend on Xist RNA but are independent of its silencing function. Together, our results suggest a role for Eed-Ezh2-mediated H3-K27 methylation during initiation of both imprinted and random X inactivation and demonstrate that H3-K27 methylation is not sufficient for silencing of the Xi.  相似文献   

高等农业院校开展双语教学存在的问题与思考     

何如海  陈涛 《安徽农业大学学报》2004,(6):85-87

实施双语教学是高等农业院校教学改革的一项新举措，它有利地开创了高等农业院校人才培养的新途径。文章从高等农业院校双语教学中课程设置、教学方法和手段、教材建设 与选用、师资力量、学生外语水平等方而分析了目前高等农业院校开展双语教学存在的实际问题与困难，提出了解决这些问题的思路与对策。  相似文献   

甘蔗育种中早代宿根选择的效应     

邓祖湖  林彦铨  林华民  陈如凯 《福建农林大学学报(自然科学版)》2002,31(2):137-141

比较不同选择途径的入选后代性状表现发现 :宿根选新植蔗 (PS)的有效茎数、小区产量和锤重明显高于新植选第 2季新植蔗 (P ) ,宿根选新植的宿根蔗 (SPS)的性状表现总体上优于新植选第 2季新植的宿根蔗 (SP ) ,SPS所有组合的小区产量均值和锤重均值都比 SP 相应组合高 ,锤度相近 .总体上 ,宿根选后代的主要性状宿根能力比新植选后代的高 .在甘蔗宿根性育种中 ,增加实生苗宿根这一环节具有重要意义 .不同选择途径的入选结果比较表明 ,只在实生苗选择单株会有很多宿根性强的基因型漏选  相似文献   

浅谈温室油菜蚜虫危害     

萨如拉 《西藏农业科技》2007,29(3):14-18

油菜蚜虫是油菜上一种常发性的害虫,常年发生较重,油菜蚜虫对油菜的危害一是直接取食为害,二是传播病毒病,世代重叠危害,严重影响产量。本文论述了油菜蚜虫的生活习性、危害发生情况、控制虫害发生的原则、防治方法等。并提出了防止油菜蚜虫危害工作的建议。  相似文献   

嗜热厌氧纤维素降解菌对纤维素的粘附及其酶活性研究     

韩如  梅建凤  闵航  陈美慈 《浙江大学学报(农业与生命科学版)》2001,27(2):165-168

本项研究表明，嗜热厌氧纤维素降解细菌Clostridium sp.EVA1在降解纤维素的过程中，菌体对不溶性纤维素底物具有强烈的粘附作用。在透明圈中参与纤维素降解的菌体周围具有波纹状突起结构，并且菌体与纤维素的粘附位点是一个电子致密区域。该菌株产生的纤维素酶是与细胞相连的胞外酶，培养液中无纤维素酶活性。以脱脂棉花为碳源培养产生的纤维素酶活性最大。该菌株纤维素酶在70℃下具有最大的酶活性。  相似文献   

富士苹果不同拉枝角度叶片营养物质含量与果实品质之间的关系   总被引：12，自引：0，他引：12  

李永武  韩明玉  范崇辉  曹儒 《西北农业学报》2007,16(2):161-164

研究了苹果树拉枝后叶片营养物质含量与果实品质之间的关系。结果表明,果树枝条经不同角度处理后,叶片含氮量随着拉枝角度的增大差异不显著,含碳量随之而增多,且拉枝角度110°与90°、70°及<55°处理间达极显著水平。果实单果重、花色素含量、硬度、总糖含量、vC含量及蛋白质含量在拉枝角度为110°时最高,总酸含量却最低。对果树叶片营养物质含量与果实品质各指标进行相关及回归分析结果表明,果树枝条经不同拉枝角度处理后叶片碳素营养物质含量与果实单果重、果形指数、花色素含量、硬度、vC含量、总糖和蛋白质含量均呈正相关,与果实总酸含量呈负相关,而且与果实总糖、蛋白质含量差异显著,与果实单果重、硬度达极显著水平。叶片氮素营养物质含量与果实单果重、果形指数、花色素含量、硬度、vC含量、总糖和蛋白质含量均呈负相关,与果实总酸含量呈正相关,且与单果重、总糖和蛋白质含量差异显著。  相似文献   

Voltammetric determination of the antioxidant capacity in wine samples using a carbon nanotube modified electrode     

Souza LP  Calegari F  Zarbin AJ  Marcolino-Junior LH  Bergamini MF 《Journal of agricultural and food chemistry》2011,59(14):7620-7625

A direct determination of gallic acid was achieved at a carbon paste electrode modified with carbon nanotubes under differential pulse voltammetry conditions. The values obtained for gallic acid were used to estimate the antioxidant properties of the wine sample based on gallic acid oxidation. The proposed method is based on the gallic acid oxidation process at a modified carbon paste electrode (MCPE) containing 30% (m/m) of carbon nanotubes monitored at 0.35 V versus Ag/AgCl (KCl 3 mol L(-1)). Using the optimized experimental conditions, the calibration curve for gallic acid was linear in the concentration range from 5.0 × 10(-7) to 1.5 × 10(-5) mol L(-1) with a detection limit of 3.0 × 10(-7) mol L(-1). The MCPE was successfully applied for the determination of the antioxidant capacity for red and white wine samples without interference of glucose and ascorbic acid, and the obtained results were compared with the standard spectrophotometric method.  相似文献