Effect of dietary vitamin A on Senegalese sole (Solea senegalensis) skeletogenesis and larval quality     

Ignacio Fernndez  Marta S. Pimentel  Juan B. Ortiz-Delgado  Francisco Hontoria  Carmen Sarasquete  Alicia Estvez  Jose Luis Zambonino-Infante  Enric Gisbert 《Aquaculture (Amsterdam, Netherlands)》2009,295(3-4):250-265

The effects of different levels of vitamin A (VA) in Senegalese sole larval performance and development were evaluated by means of a dietary dose–response experiment using enriched Artemia metanauplii as a carrier of this micronutrient. Larvae were fed from 6 to 27 days post hatch (dph) with enriched Artemia containing graded levels of total VA (1.3, 2.1, 4.5 and 12.9 µg VA mg^− 1 DW). The content of VA in live prey directly affected its accumulation in larvae and early juveniles. Retinyl palmitate accumulated during larval ontogeny, whereas retinol showed the opposite trend, decreasing from hatching until 41 dph and then remaining constant until the end of the study.In metamorphic larvae (10 and 15 dph), VA did not affect the number of thyroid follicles or the intensity of the immunoreactive staining of T₃ and T₄. However, at older stages of development (post-metamorphic larvae: 20, 30, 41 and 48 dph), VA decreased the number of thyroid follicles but increased their mean size and enhanced T₃ and T₄ immunoreactive staining. A dietary excess of VA did not affect either larval performance in terms of growth and survival or the maturation of the digestive system. However, the most remarkable impact of this morphogenetic nutrient was detected during skeletal morphogenesis. Dietary VA accelerated the intramembranous ossification of vertebral centrums, which led to the formation of a supranumerary haemal vertebra and a high incidence of fused and compressed vertebrae in fish fed 2.1, 4.5 and 12.9 mg VA mg^− 1 DW. In addition, VA also affected those structures from vertebrae and caudal fin formed by chondral ossification, leading to defects in their shape and fusions with adjacent skeletal elements. In particular, the caudal fin was the region most affected by the dietary treatments. In order of importance, the bones with more developmental anomalies were the modified neural and haemal spines, epural, hypurals and parahypural. The impact of systemic factors such as thyroidal hormones in skeletogenesis should not be neglected since present results revealed that an excess of dietary VA affected the levels of T₃ and T₄, which might have affected bone formation and remodelling, leading to skeletal deformities.  相似文献   

Migration performance of wild and hatchery sea trout (Salmo trutta L.) smolts—Implications for compensatory hatchery programs     

Ignacio Serrano  Stefan Larsson  Lars-Ove Eriksson 《Fisheries Research》2009,99(3):210-215

Migration success of hatchery-reared and wild sea trout smolts through the lower stretches and the estuary of a Baltic Sea river were studied. During 3 years, wild and hatchery trout smolts were implanted with acoustic transmitters and released 14 km upstream from the river mouth. In order to monitor their out-migration pattern, acoustic receivers were deployed along the migratory route. Data on number of fish detected and date and time of detections were analysed and the migratory performance of wild and hatchery-reared fish was compared. A significantly higher proportion of wild fish (80%) successfully migrated to the coast compared to fish of hatchery origin (27.5%) and migration was faster in wild smolts. Hatchery fish were larger and had a higher condition factor and lipid concentrations, which are proposed as possible reasons for the poorer migratory performance of the hatchery-reared fish.  相似文献   

Aetiology of branch dieback,panicle and shoot blight of pistachio associated with fungal trunk pathogens in southern Spain     

Ana López-Moral  María del Carmen Raya  Cristina Ruiz-Blancas  Ignacio Medialdea  María Lovera  Octavio Arquero  Antonio Trapero  Carlos Agustí-Brisach 《Plant pathology》2020,69(7):1237-1269

Pistachio represents an emerging nut crop across the Mediterranean basin. In Spain, pistachio has been traditionally cultivated in marginal-dry areas with unfavourable climatic conditions for plant diseases. Consequently, little attention has been given to research on pistachio diseases until recently. Symptoms of branch dieback and cankers, and shoot and panicle blight have been recently observed in commercial pistachio orchards across southern Spain. In this study, 10 commercial pistachio orchards showing disease symptoms were surveyed between 2017 and 2018. Botryosphaeriaceae fungi were consistently isolated from affected shoots, among other fungal families with minor relevance. Representative isolates of each family were characterized based on colony and conidial morphology, optimum growth temperature, and the comparison of DNA sequence data (ITS, LSU, EF, TUB2, and ACT genomic regions). Detached and attached shoots, and attached panicles of pistachio cv. Kerman were inoculated with mycelial plugs or conidial suspensions to demonstrate the pathogenicity of the selected isolates. Botryosphaeria dothidea, Lasiodiplodia pseudotheobromae, Neofusicoccum mediterraneum, N. parvum, Diaporthe neotheicola, Diaporthe sp., Eutypa lata, Eutypa sp., Cytospora sp., and Phaeoacremonium minimum were identified. P. minimum had the highest optimum growth temperature (29.6 °C) and Cytospora sp. the lowest (21–22 °C). Botryosphaeriaceae isolates showed the largest lesions on inoculated shoots, with L. pseudotheobromae being the most aggressive, followed by Neofusicoccum species. Panicles inoculated with N. mediterraneum showed blight symptoms and canker formation 6 weeks after inoculation, without significant differences in aggressiveness between isolates. This work reports relevant information about this emerging disease in the novel Spanish pistachio-growing areas.  相似文献   

Stress response in silver catfish (Rhamdia quelen) exposed to the essential oil of Hesperozygis ringens     

Cândida Toni  Juan Antonio Martos-Sitcha  Ignacio Ruiz-Jarabo  Juan Miguel Mancera  Gonzalo Martínez-Rodríguez  Carlos Garrido Pinheiro  Berta Maria Heinzmann  Bernardo Baldisserotto 《Fish physiology and biochemistry》2015,41(1):129-138

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Evaluation of bacterial growth,effects on albumin,and coagulation factors in canine fresh frozen plasma administered as continuous rate infusion exposed to room temperature for 12 hours     

Ignacio Mesa-Sanchez DVM  PhD  DECVIM  Rui R. F. Ferreira DVM  PhD  Carles Blasi-Brugué DVM  Rafael R. de Gopegui DVM  PhD  DECVIM  Augusto J. F. de Matos DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2023,33(5):534-539

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Climatic signal of earlywood vessels of oak on a maritime site   总被引：1，自引：0，他引：1  

González IG  Eckstein D 《Tree physiology》2003,23(7):497-504

Earlywood vessel lumen areas were measured in 72 consecutive tree rings in wood cores from oak (Quercus robur L.) trees in a maritime woodland. This anatomical time series was statistically correlated with climate data for the same time span. There was a strong dependence of earlywood vessel lumen area on rainfall between February and April, which reflects the role of water availability in vessel ontogeny. By inversion, earlywood vessel lumen areas can be used as a proxy to reconstruct spring precipitation beyond the archived weather records. Such information may be of value in the context of climate change.  相似文献   

Estimating the sustainable harvesting and the stable diameter distribution of European beech with projection matrix models     

Ignacio López  Sigfredo Francisco Ortuño  Ángel Julián Martín  Carmen Fullana 《Annals of Forest Science》2007,64(6):593-599

We present a projection matrix model to estimate the sustainable harvest rates and the stable diameter distributions of three qualities of European beech in the Spanish province of Navarre. Considering a period of 10 years and the diameter growth, trees were grouped into five classes: (0,10), (10,20), (20,30), (30,40) and over 40 cm. The transition probabilities were calculated assuming an approximation by splines to the diameter growth curves and uniform distributions for the diameters in each class. A condition for sustainable harvesting, leading to reach in each harvest the stable diameter distribution, was introduced. The results obtained suggest that, for each projection and depending on the quality, harvest rates in the range 18.8–37.5% for recruitments in the range 200–840 stems/ha, may be sustained without risk of a population reduction. Finally, the stable diameter distributions in relation to the recruitment were also obtained for each quality.  相似文献   

Growth and yield modelling of <Emphasis Type="Italic">Acacia mangium</Emphasis> in Colombia     

Danny Alexander Torres Vélez  Jorge Ignacio Del Valle 《New Forests》2007,34(3):293-305

To study the growth and yield of Acacia mangium in the Caribbean region of Colombia, allometric equations of total volume and aboveground plus coarse roots biomass were fitted as a function of the tree’s diameter at breast height (dbh). The von Bertalanffy’s growth model and 59, 0.1 ha plots (0.55–9.55 years old) were used to develop site index (SI) curves at 6 years base age. Then, using the state-space approach, stand growth and yield models were developed for basal area, volume and biomass. The results show that A. mangium is a very promising species for timber production, atmospheric carbon removal and soil restoration because it grows very fast even in mining degraded soils. On average sites it reaches 15 m in height in 3 years. However, early and reiterated thinning coupled with initial mortality by cattle invasion of the very young understocked plantations are producing relatively low yields.  相似文献   

Epidemiological surveillance of bluetongue virus serotypes 1, 4 and 8 in Spanish ibex (Capra pyrenaica hispanica) in southern Spain     

Lorca-Oró C  Pujols J  Arenas A  Gómez-Guillamón F  Zorrilla I  Domingo M  Arenas-Montés A  Ruano MJ  García-Bocanegra I 《Veterinary microbiology》2011,149(1-2):230-235

A cross-sectional study was carried out to assess the prevalence and circulation of bluetongue virus (BTV) in Spanish ibexes (Capra pyrenaica hispanica). A total of 770 sera samples, 380 blood samples and 34 spleen samples were collected between 2006 and 2009 in Andalusia (southern Spain), a region and time period with a wide circulation of BTV in livestock. Thirty-one out of 770 (4.0%; CI(95%): 2.6-5.4) sera samples analyzed by ELISA showed antibodies against BTV. Twenty-four out of 31 seropositive samples were tested against BTV serotypes 1, 4 and 8 by serum neutralization test (SNT). Neutralizing antibodies against BTV-1 and BTV-4 were detected in seven and ten animals, respectively, four of them showed neutralizing antibodies to both serotypes. The animals seropositive to BTV-4 were sampled between 2006 and 2008, while BTV-1 circulation was confirmed in ibexes sampled between 2007 and 2009. None of the ibexes presented neutralizing antibodies against BTV-8. Statistically significant differences were found among regions and years, which is in coincidence with what occurred in domestic ruminants. There were no statistically significant differences between sexes, age classes and habitats (captivity vs. free-living). BTV RNA was not found in any of the 380 blood samples analyzed. However, BTV-1 RNA was detected from spleen in one Spanish ibex from Málaga province in August 2008. This finding evidences the presence of BTV-1 in Spanish ibex in a municipality where BT outbreaks were not detected in domestic ruminants during that period. Results of the present study show that Spanish ibexes were exposed and responded serologically to both BTV-1 and BTV-4. The low seroprevalence obtained suggests that Spanish ibex is not a relevant species in the dissemination of BT. However, the detection of BTV-1 RNA and the presence of seropositive ibexes in areas where BT outbreaks were not detected in livestock, could not exclude a significant role in the epidemiology of BTV in certain areas.  相似文献   

Divergent Strategy in Marine Tetracyclic Meroterpenoids Synthesis     

Antonio Rosales Martínez  Ignacio Rodríguez-García  Josefa L. Lpez-Martínez 《Marine drugs》2021,19(5)

The divergent total synthesis strategy can be successfully applied to the preparation of families of natural products using a common late-stage pluripotent intermediate. This approach is a powerful tool in organic synthesis as it offers opportunities for the efficient preparation of structurally related compounds. This article reviews the synthesis of the marine natural product aureol, as well as its use as a common intermediate in the divergent synthesis of other marine natural and non-natural tetracyclic meroterpenoids.  相似文献