Sodium bicarbonate protects uranium-induced acute nephrotoxicity through uranium-decorporation by urinary alkalinization in rats     

Yasushi Ohmachi  Tomomi Imamura  Mizuyo Ikeda  Eriko Shishikura  Eunjoo Kim  Osamu Kurihara  Kazuo Sakai 《Journal of toxicologic pathology》2015,28(2):65-71

To evaluate the effectiveness of sodium bicarbonate (SB) in removing uranium and protecting animals from uranium toxicity, we intramuscularly administered 1 mg/kg of uranyl nitrate to 8-wk-old male SD rats, and 20 min after administration of uranyl nitrate, the animals were given a single oral administration of SB at 0.1, 0.3 or 1 g/kg. The SB treatment at a dose of 0.3 g/kg or more raised the pH of the rats’ urine until 4 h after treatment, and it significantly reduced the uranium amounts in the kidneys at 1 day after treatment. In another experiment, rats were intramuscularly administered 1 mg/kg of uranyl nitrate, and 20 min later, the animals were treated with sodium bicarbonate (0.1 or 1 g/kg). The rats were autopsied at 1, 3 and 7 days after uranium treatment. High-dose SB resulted in a significant increase in urinary uranium excretion in the first 24 h and a reduction of uranium deposition in the kidneys and femurs, and it also significantly suppressed uranium-induced renal toxicity, as shown by both histopathology and clinical chemistry at 3 days after uranium treatment. Low-dose SB did not show such marked effects. Our findings demonstrated that the uranium decorporation effect of sodium bicarbonate was observed at the dosage showing urine alkalinization in rats and that decorporation effect of sodium bicarbonate might be beneficial if it is administered immediately after incorporation of soluble uranium.  相似文献   

Osmo-responsive expression of oyster amino acid transporter gene and analysis of the regulatory region involved     

Haruhiko?ToyoharaEmail author  Masashi?Ikeda  Chika?Goto  Hideki?Sawada  Masatomi?Hosoi  Kazuaki?Takeuchi  Isao?Hayashi  Shintaro?Imamura  Michiaki?Yamashita 《Fisheries Science》2005,71(3):465-470

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Effects of dihydroheptaprenol on neutrophil functions in calves   总被引：1，自引：0，他引：1  

O Yoneyama  S Osame  S Ichijo  M Kimura  S Araki  M Suzuki  E Imamura 《The British veterinary journal》1989,145(6):531-537

Microemulsified dihydroheptaprenol (DHP) was administered intramuscularly to clinically healthy calves at doses of 4, 6 and 8 mg/kg body weight. Marked increase of neutrophil counts was observed 0.5, 1 and 2 days after the injection at a dose of 6 mg/kg. Nitroblue tetrazolium reducing activity of neutrophils was also obviously enhanced 1, 2 and 3 days after DHP administration. Phagocytic killing of Staphylococcus aureus by neutrophils was further enhanced markedly 1, 2 and 3 days after the drug injection at 6 mg/kg.  相似文献   

Establishment of a continuous cell line derived from leukaemic cattle     

H Koyama  Y Imamura  H Yoshikawa  O Kajikawa  S Itohara  Y Mizuno  T Yoshikawa  H Saito 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1986,33(8):586-596

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Liquid penetration of precompressed wood VI: Anatomical characterization of pit fractures   总被引：3，自引：0，他引：3  

Ugai Watanabe  Yuji Imamura  Ikuho Iida 《Journal of Wood Science》1998,44(2):158-162

Pit fractures of refractory coniferous heartwoods caused by precompression in the radial direction were investigated and are discussed in terms of improved liquid penetration. Small cracks appeared at the boundary between the torus and margo, along the outer margin of the margo, and on the torus when specimens were compressed and deformation was fixed by drying. The remarkable cracks were generally observed for Cryptomeria japonica D. Don. Pseudotsuga menziesii Franco showed peculiar detachment of the torus from the pit border, and Larix leptolepis Gordon exhibited only small cracks on the torus. These fractures patterns were clearer when the precompressed specimens were recovered by water impregnation and then redried.This report was presented at the 46th Annual Meeting of the Japan Wood Research Society, Kumamoto, April 1996  相似文献   

Selenoneine suppresses melanin synthesis by inhibiting tyrosinase in murine B16 melanoma cells and 3D-cultured human melanocytes     

Seko  Takuya  Imamura  Shintaro  Ishihara  Kenji  Yamashita  Yumiko  Yamashita  Michiaki 《Fisheries Science》2020,86(1):171-179

Fisheries Science - The novel organic selenium compound, selenoneine, is found in the blood of tuna and has metal-binding activity. In this report, selenoneine displays tyrosinase inhibitory...  相似文献   

Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3     

Megumi Yamagishi  Ritsuko Hosoda-Yabe  Hideki Tamai  Miku Konishi  Akihiro Imamura  Hideharu Ishida  Tomio Yabe  Hiromune Ando  Makoto Kiso 《Marine drugs》2015,13(12):7250-7274

LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.  相似文献   

Origin identification of dried seaweed product “nori” by PCR–RFLP analysis of Pyropia yezoensis in the internal transcribed spacer ITS-1 region     

Ken Touhata  Atsushi Namikoshi  Tamami Suzuki  Jun Iguchi  Nanami Mizusawa  Tatsuro Hara  Shintaro Imamura  Takeshi Yabu  Yumiko Yamashita  Michiaki Yamashita 《Fisheries Science》2013,79(5):865-875

Nucleotide sequences in internal transcribed spacer (ITS)-1 region derived from dried nori products produced in Japan, China, and the Republic of Korea were compared. Thalli contained in the Japanese products were genetically homogenous, and their nucleotide sequences in ITS-1 were identical to those of the reference strains of Pyropia yezoensis f. narawaensis. In Chinese products, the thalli were related to P. yezoensis strain Minomiasakusa. In contrast, the thalli in the Korean products were genetically heterogeneous, and several different P. yezoensis strains and other Pyropia spp. were used for dried nori products. In some thalli produced in both China and Korea, the DNA sequences of the ITS-1 region were identical with that of Japan, suggesting that the cultivar strains might have been transplanted from Japan to China in recent years. The 432-bp-long nucleotide sequences in the ITS-1 region of thalli derived from Japanese origin were cleaved to two restriction fragments at 154 and 278 bp by cleavage of PCR-amplified products using MspI. Conversely, almost all of the corresponding sequences derived from China and Korea were lacking MspI or other restriction patterns, except for nori products from some areas that cultivate a closely related strain to the Japanese cultivar.  相似文献   

Anatomical characterization of decayed wood in standing light red meranti and identification of the fungi isolated from the decayed area     

Erwin  Shuhei Takemoto  Won-Joung Hwang  Miyuki Takeuchi  Takao Itoh  Yuji Imamura 《Journal of Wood Science》2008,54(3):233-241

To further our understanding of wood decay in living light red meranti (Shorea smithiana) trees, microscopic characteristics of the cell and cell wall degradations of S. smithiana wood in the presence of the decay fungi, the identity of the causal fungi, and the decay potential and pattern by an isolated fungus were investigated. Cell wall degradations, including cell wall thinning, bore holes formation, rounded pit erosion, and eroded channel opening were clearly observed under light and scanning electron microscopy. In transverse view, many large voids resulting from a coalition of degraded wood tissue appeared in the decayed canker zone. All these observations suggest the well-known simultaneous decay pattern caused by white-rot fungi. By phylogenetic analysis based on the sequences of internal transcribed spacer region of ribosomal DNA, a basidiomycete fungus isolated from the decayed wood was identified as Schizophyllum commune. The degradation caused by this fungus on sound S. smithiana wood in an in situ laboratory decay test was classified as the early stage of simultaneous decay, and showed a similar pattern to that observed in the wood samples naturally decayed.  相似文献   

A novel control strategy for dry-wood termite <Emphasis Type="Italic">Incisitermes minor</Emphasis> infestation using a bait system     

Yuliati Indrayani  Tsuyoshi Yoshimura  Yuji Imamura 《Journal of Wood Science》2008,54(3):220-224

Three types of experiments were designed to evaluate the performance of a bait system intended to control Incisitermes minor (Hagen). In the first type of experiment, Type I, the effectiveness of the bait in a small wood specimen was evaluated. In the second type, Type II, the bait effectiveness was evaluated in a larger wood specimen. Feeding arena lumber with artificial galleries was prepared for the Type III experiment so that the response of the insects to the gel could be observed. In general, the average percentage of termites that died after being exposed to the gel formulation in all three types of experiment was more than 60%, and in the gel control the average percentage of live termites was more than 95% in Types I and III, and more than 75% in Type II. These results suggest that the gel bait system used in this study has the potential to eliminate I. minor colonies. Further investigation will be necessary to increase the reliability of the bait system as a control measure against dry-wood termites.  相似文献