Serum testosterone,sperm quality,cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly          下载免费PDF全文

A Aquino‐Cortez  BQ Pinheiro  HVR Silva  DBC Lima  TFP Silva  MB Souza  DA Viana  FAF Xavier Júnior  JSAM Evangelista  FZ Brandão  LDM Silva 《Reproduction in domestic animals》2017,52(6):998-1003

Prostatomegaly is a common finding in older non‐neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm‐rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction. Dogs with prostatomegaly have the lowest sperm motility, vigour, concentration and functional membrane. Dogs with prostatomegaly have the highest glucose, triglycerides and cholesterol. Glucose was the only constituent positively correlated with serum testosterone and prostate volume. It can be concluded that dogs with prostatomegaly have poorer sperm quality, and glucose, triglycerides and cholesterol in prostatic fraction can be used as prostatomegaly biomarkers.  相似文献   

Development of a multiplex RT-PCR assay for simultaneous detection of the major viruses that affect rainbow trout (Oncorhynchus mykiss)     

Ana C. A. S. Pinheiro  Enrico Volpe  Donatella Principi  Santino Prosperi  Sara Ciulli 《Aquaculture International》2016,24(1):115-125

The major viral diseases that affect rainbow trout (Oncorhynchus mykiss) are viral haemorrhagic septicaemia, infectious haematopoietic necrosis, infectious pancreatic necrosis and sleeping disease. In the presented study, we developed a multiplex RT-PCR (mRT-PCR) assay for the simultaneous detection of these four rainbow trout viruses in a single assay. The choice of primers was carried out based on the expected size of the fragments, the temperature and time required for the amplification, and the specificity for the target sequence. Firstly, the method was optimised using reference strains of viral haemorrhagic septicaemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV) and sleeping disease virus (SDV) cultivated with permissive cell culture lines; subsequently, the method was used for the identification of these viral infections in rainbow trout samples. Twenty-two samples of rainbow trout, clinically suspected of having viruses, were analysed by the developed method to detect the presence of the four viruses, by directly analysing the animal tissues. The mRT-PCR method was able to efficiently detect the viral RNA in infected cell culture supernatants and in tissue samples, highlighting the presence of single infections as well as co-infections in rainbow trout samples. VHSV/SDV and IHNV/SDV co-infections were demonstrated for the first time in rainbow trout. The mRT-PCR method was revealed to be an accurate and fast method to support traditional diagnostic techniques in the diagnosis of major viral diseases of rainbow trout.  相似文献   

Evaluation of the maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) diversity on the Archipelago of Madeira     

Miguel Ângelo A. Pinheiro de Carvalho  José Filipe Teixeira Ganança  Ivo Abreu  Nélia F. Sousa  Teresa M. Marques dos Santos  Maria Rita Clemente Vieira  Mario Motto 《Genetic Resources and Crop Evolution》2008,55(2):221-233

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Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA   总被引：2，自引：0，他引：2  

Pinheiro AM  Costa MF  Paule B  Vale V  Ribeiro M  Nascimento I  Schaer RE  Almeida MA  Meyer R  Freire SM 《Veterinary parasitology》2005,130(1-2):73-79

Neospora caninum, is a coccidian protozoan known as a major cause of bovine abortion and canine neuropathies. The aim of the present study was to develop a reliable and quick test to detect antibodies to N. caninum in dog sera. Sixty-five serum samples from dogs, including 35 positive and 30 negative for N. caninum antibodies were used for standardization of the test. In parallel, immunoreactivity of the sera to Toxoplasma gondii antigens was investigated using a passive agglutination test. A dot-ELISA test, using soluble extract of N. caninum tachyzoites on nitrocellulose ester membranes, was developed and standardized. SDS-PAGE and complementary analysis of reactivity by Western blotting were used for the characterization of the immunoreactive fractions of all tested sera. The sensitivity and specificity of the dot-ELISA were 94 and 73%, respectively, compared to IFAT at a cut-off of 1:50, and 87 and 100% compared to IFAT at a cut-off of 1:25. Among the sera that tested positively for both IFAT and dot-ELISA, only 8.6% were reactive to T. gondii. The most immunoreactive fractions in Western blots were the 14-, 33-, 42- and 55 kDa bands, with percentages of 42, 60, 42 and 37%, respectively. The 60 kDa band showed a non-specific reaction in 43% of neosporosis-negative animals by both dot-ELISA and IFAT. These results indicate that the dot-ELISA using N. caninum antigen present good sensitivity and specificity, and might be used as a screening test to detect antibodies to N. caninum in dogs.  相似文献   

Genotyping of Toxoplasma gondii strains isolated from dogs with neurological signs     

da Silva AV  Pezerico SB  de Lima VY  d'Arc Moretti L  Pinheiro JP  Tanaka EM  Ribeiro MG  Langoni H 《Veterinary parasitology》2005,127(1):23-27

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Light and scanning electron microscopy of the miracidium of Echinostoma paraensei (Trematoda, Echinostomatidae)     

Pinheiro J  Maldonado A  Lanfredi RM 《Veterinary parasitology》2004,121(3-4):265-275

Echinostoma paraensei was described 1967 by Lie and Basch. Recently its natural definitive host, the aquatic rodent Nectomys squamipes, endemic in Brazil, was identified. As most of the echinostomatids, this species presents a zoonotic potential. The morphology and topography of the E. paraensei miracidium obtained from adult worms collected from the natural definitive host N. squamipes is described by light and electron microscopy. The arrangement and the dimensions of the epidermal plates are given. The eyespots are composed by two pairs of lenses measuring 6.03 microm. SEM observations shows that the miracidium body is covered by cilia except at the terebratorium region and the presence of alpha-tubulin in the cilia of the larvae is first recorded by immunelabeling. Nineteen papilla-like structures arranged in three axes and four groups were observed at the terebratoriun, this structure is retractable, presenting folds and a corrugated surface with profiles of cytoplasmic expansions, forming network of anatomizing folds.  相似文献   

Genital lesions and distribution of amastigotes in bitches naturally infected with Leishmania chagasi     

Silva FL  Rodrigues AA  Rego IO  Santos RL  Oliveira RG  Silva TM  Xavier MN  Nascimento EF  Santos RL 《Veterinary parasitology》2008,151(1):86-90

Recent reports indicate that Leishmania chagasi has tropism to the male canine genital system, which is associated with shedding of the organism in the semen, supporting the hypothesis of venereal transmission. The aim of this study was to describe the lesions and assess parasite load in the genital system of bitches with canine visceral leishmaniasis (CanL). Symptomatic (n=5) and asymptomatic (n=5) bitches seropositive for CanL were randomly selected at the Center for Zoonosis Control (Belo Horizonte, State of Minas Gerais, Brazil). Five serologically negative, healthy, adult bitches also from the CZC were used as controls. Samples from genital organs (vulva, vagina, cervix, uterine body, uterine horns, uterine tubes, and ovaries), liver, and spleen were histologically evaluated and processed for immunodetection of Leishmania sp., and PCR. The most significant histological change was a mild to moderate vulvar dermatitis, characterized by a histio-plasma-lymphocytic infiltrate. This change was detected in all asymptomatic, four symptomatic, and three uninfected control bitches. In one symptomatic and one asymptomatic bitch intracytoplasmic amastigotes were observed within macrophages in the inflammatory infiltrate. Samples from all the segments of the genital tract were positive in at least one infected animal, in the absence of detectable amastigotes in the tissue. These findings support the notion that L. chagasi does not have genital tropism in the bitch, which is in contrast to our previous findings in naturally infected male intact dogs.  相似文献   

A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection     

Rosivaldo Quirino Bezerra Júnior  Ângela Maria Xavier Eloy  João Ricardo Furtado  Raymundo Rizaldo Pinheiro  Alice Andrioli  Frederico Bruno Moreno  Marina Duarte Pinto Lobo  Ana Cristina O. Monteiro-Moreira  Renato de Azevedo Moreira  Tatiana Maria Farias Pinto  Maria Fátima da Silva Teixeira 《Tropical animal health and production》2018,50(1):43-48

The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE.  相似文献   

Long-distance transport of hair lambs: effect of location in pot-belly trailers on thermo-physiology,welfare and meat quality     

G. C. Miranda-de la Lama  M. Rodríguez-Palomares  R. G. Cruz-Monterrosa  A. A. Rayas-Amor  R. S. B. Pinheiro  F. M. Galindo  M. Villarroel 《Tropical animal health and production》2018,50(2):327-336

Livestock transport exposes animals to a range of potential stressors that may compromise their welfare and final product quality, and those effects typically increase with the distance travelled. In North America, producers often use pot-belly vehicles for long hauls but little is known about their suitability for transporting lambs. We followed two long-distance trips using pot-belly trailers carrying 500 lambs (each) from Northern to Central Mexico in winter, to measure possible effects on animal welfare and meat quality. Sixty lambs per trip were placed at different locations within the pot-belly trailer. Animals were equipped with iButton Thermochron® temperature devices, which registered body temperature throughout pre- and post-slaughter stages. Despite the rather cool winter conditions, lambs placed in the “belly” and “nose” compartments had higher body temperatures at loading, during transport and lairage and after slaughter (carcass temperature). Those lambs also had higher levels of plasma cortisol, glucose and creatine kinase (CK), and a higher neutrophil/lymphocyte ratio. Regarding meat quality, ultimate pH (24 h) was higher in all locations, especially in LT4 location. Overall, the results suggest a link between thermal stress during transport, elevated physiological indicators of stress and poorer meat quality.  相似文献   

Prevalence of subclinical hypocalcemia in dairy cows in the Sousa city micro-region,Paraíba state     

da Silva  Daniel Cézar  Fernandes  Beatriz Dantas  dos Santos Lima  Jessica Monique  Rodrigues  Gilderlândio Pinheiro  Dias  Déborah Lanne Barros  de Oliveira Souza  Evaristo Jorge  Filho  Miguel Arcanjo Moreira 《Tropical animal health and production》2019,51(1):221-227

Tropical Animal Health and Production - Subclinical hypocalcemia is a metabolic disorder characterised by a decrease in serum calcium (Ca) levels, with no clinical symptoms. The aim of this study...  相似文献