Method for Precision Nitrogen Management in Spring Wheat: II. Implementation     

Long  Dan S.  Engel  Richard E.  Carlson  Gregg R. 《Precision Agriculture》2000,2(1):25-38

By accounting for spatial variation in soil N levels, variable-rate fertilizer application may improve crop yield and quality, and N use efficiency within fields. The main purpose of this study was to demonstrate how site-specific wheat yield and protein data, and a geographic information system may be used in developing precision N-recommendations for spring wheat. The three steps in the procedure include: (1) estimate the amount of N-removed in wheat in the year in which the crop is harvested, (2) estimate the N-deficit, defined as the amount of additional N needed for raising protein concentration in a future crop to a specified target level, and (3) estimate the total N-recommendation by summing the mapped values of the N-removed and the N-deficit. A map for variable-rate application of fertilizer is derived by specifying cutoff values to divide the range in the total N-recommendation into classes representing N management zones.A field experiment was conducted within an annually cropped wheat field (101 ha) in northern Montana to determine whether the proposed method could improve grain yields and protein levels. The N-removal and N-deficit were estimated from site-specific wheat yield and protein data that were acquired during harvest of 1996. In 1997, which was a dry year, an experiment was conducted in the same field that consisted of a randomized complete block design arranged as pairs of strip plots. Variable- or uniform-rate N treatments were randomly assigned to each pair of strips. Both treatments received nearly the same amount of fertilizer, however, N in the variable treatment was varied to match patterns in grain yield and protein levels that previously existed in 1996. Yields were not significantly different between management systems, but proteins were significantly enhanced by spatially variable N application. In addition, variability in protein levels was reduced within the whole field. Field areas deficient in N fertility could be identified without having to sample for soil profile N.  相似文献   

Isolation of very virulent Marek''s disease viruses from vaccinated chickens in Australia     

JL McKIMM-BRESCHKIN  JT FARAGHER  J WITHELL  WM FORSYTH † 《Australian veterinary journal》1990,67(6):205-209

Very virulent Marek's disease viruses (vvMDV), defined as isolates against which the herpesvirus of turkey (HVT) vaccine provide poor protection, have been isolated from poultry flocks in both the United States and Europe. Twenty-one samples from vaccinated Australian flocks, experiencing problems with excessive Marek's disease (MD), were tested for the presence of transmissible MD viruses (MDV). Of the 16 samples which contained a transmissible agent, 14 were pathogenic in chickens, based on the development of MD lesions or depression of the bursa/body weight ratio. Of the pathogenic isolates which have been successfully typed 10 were serotype 1, and one was serotype 2 MDV. Pathogenicity of isolates varied. Several isolates caused tumours in 20-30% of both vaccinated and unvaccinated chickens. Two isolates, MPF6 and MPF23, caused tumours in more than 50% of chickens. When MPF6 and MPF23 were tested in vaccine trials bivalent vaccine gave no better protection against development of MD lesions than a monovalent vaccine. Isolate MPF23 was so pathogenic that lesions were produced in all chickens, regardless of the vaccine protocol used. Therefore vvMDV have been isolated in Australia, and unlike the vaccines tested overseas, bivalent Australian vaccines do not appear to provide greater protection against these vvMDV.  相似文献   

Necrotic hepatitis of rabbits in Mexico: a parvovirus   总被引：2，自引：0，他引：2  

D A Gregg  C House 《The Veterinary record》1989,125(24):603-604

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Equine neonatal septicaemia: 24 cases     

AL RAISIS  JL HODGSON  DR HODGSON 《Australian veterinary journal》1996,73(4):137-140

Equine neonatal septicaemia was confirmed in 24 foals hospitalised at the Rural Veterinary Centre between 1989 and 1992 with suspected septicaemia. Septicaemia was confirmed by culture of bacteria from blood of live foals and tissues obtained at necropsy of foals that died or were euthanased. Pathogenic bacteria isolated were predominantly Enterobacteriaceae (including Escherichia coli and Salmonella serovars) and Actinobacillus equuli. Clinical manifestations of septicaemia included signs of depression, dehydration, abnormalities in body temperature and manifestations of localised infection including diarrhoea, pneumonia, and septic arthritis. Most common haemato-logical abnormalities were neutropenia and increase of circulating band neutrophils. Survival rate of foals with confirmed septicaemia was 70.8%. Survival was found to be less likely in the presence of pneumonia, severe signs of depression, marked haematological changes or septic arthritis at the time of admission. Seven foals were confirmed to have septic arthritis without concurrent septicaemia. Of these, 4 had multiple joint involvement. Bacteria isolated from infected joints were predominantly Salmonella serovars. Four foals with septic arthritis failed to survive, due to multiple joint infection, which was unresponsive to treatment. The clinical and haematological abnormalities present in foals with confirmed septicaemia and septic arthritis were consistent with those observed in other studies. The bacterial isolates from foals with confirmed septicaemia were similar to those isolated in other studies. In contrast, the bacteria isolated from foals with septic arthritis without concurrent septicaemia were different from other studies.  相似文献   

Digestible tryptophan requirements of starting, growing, and finishing pigs.   总被引：6，自引：0，他引：6  

K G Burgoon  D A Knabe  E J Gregg 《Journal of animal science》1992,70(8):2493-2500

Seven hundred eight crossbred pigs were used in growth and digestion trials to determine the digestible tryptophan (Trp) requirement of starting (6 to 16 kg), growing (22 to 50 kg), and finishing (55 to 97 kg) pigs. Each growth trial evaluated a corn-fish meal-corn gluten meal basal diet, the basal diet with five incremental additions of L-Trp, and a control corn-soybean meal diet. The tryptophan content of the six incremental diets ranged from .13 to .255% for starting pigs, .08 to .18% for growing pigs, and .063 to .163% for finishing pigs. Lysine contents of basal diets were 1.38, .90, and .72% for starting, growing, and finishing diets, respectively. In all trials, ADG, ADFI, and gain/feed increased (P less than .001) linearly and quadratically as dietary Trp increased. Broken-line regression analyses determined the total dietary Trp requirements needed to optimize performance to be .19, .13, and .09% (as-fed basis) for starting, growing, and finishing pigs, respectively. These concentrations equated to Trp intakes of .96, 2.18, and 2.88 g/d. A digestion trial using growing pigs (29 kg initially) determined the apparent ileal digestibility of Trp in the basal starting and growing diets to be 72 and 70%, respectively; a similar trial with finishing pigs (55 kg initially) found 59% Trp digestibility for the basal finishing diet. Mean digestibility of L-Trp was 97%. Based on these values and the total Trp requirements given above, the digestible Trp requirements are .15, .10, and .06% for starting, growing, and finishing pigs, respectively.  相似文献   

Effects of Growth Hormone on In Situ Culture of Bovine Preantral Follicles are Dose Dependent          下载免费PDF全文

CR Jimenez  JL de Azevedo  RG Silveira  J Penitente‐Filho  EL Carrascal‐Triana  AM Zolini  VR Araújo  CAA Torres 《Reproduction in domestic animals》2016,51(4):575-584

The objective of this study was to evaluate different concentrations of growth hormone (GH) on the development of bovine preantral follicles cultured included in the ovarian tissue (in situ) on the rates of morphologically normal, viable, primordial and developing follicles, as well as the oocyte and follicle diameter and ultrastructural analysis. Ovarian fragments collected from cows with no cross‐breeds defined were cultured in situ for 1 and 7 days in minimal essential medium (α‐MEM+) supplemented with different concentrations of recombinant human GH (0, 10, 25, 50 ng/ml). The ovarian fragments non‐cultured (control) and cultured were processed for classic histology, mechanical isolation and electron transmission microscopy (MET). The parameters underwent anova (Tukey′s and Dunnett′s tests) and chi‐square test (χ²). After 7 days of culture, the treatment with 50 ng/ml GH showed no differences with fresh control (p > 0.05) and had greater effectiveness than in the 0, 10 and 25 ng/ml GH concentrations of the morphologically normal follicles. Regarding the primordial follicles, a reduction was observed in the 50 ng/ml GH concentration concomitant with the significant increase in developing follicles, differing from both the fresh control and the other GH concentrations tested. In addition, 50 ng/ml GH showed a larger follicle and oocyte diameter when compared to the other treatments cultured. Similar structures were ultrastructurally observed in the control group, 50 ng/ml GH. Follicles cultured in 10 ng/ml GH showed nuclear invagination, vacuoles and lesioned basal membrane. Hence, it is concluded that 50 ng/ml GH is the most effective concentration for the development of preantral follicles cultured in situ.  相似文献   

Long‐term Effects of Pyrethrin and Cyfluthrin,a Type II Synthetic Pyrethroid,Insecticide Applications on Bull Reproductive Parameters          下载免费PDF全文

JL Stewart  CF Shipley  FA Ireland  VL Jarrell  CL Timlin  DW Shike  TL Felix 《Reproduction in domestic animals》2016,51(5):680-687

The objectives of this study were to determine effects of cyfluthrin and pyrethrin spray products, used in combination with cyfluthrin topical and ear tag applications, on bull reproductive parameters over 18 weeks. Angus or Angus x Simmental bulls were randomly assigned to one of three treatment groups: (i) no exposure to pyrethrins/cyfluthrin (CONT; n = 10), (ii) cyfluthrin ear tag and topical applications (ET; n = 10), or (iii) cyfluthrin ear tag, topical, premise spray and pyrethrin fog spray applications (ET+S; n = 8). Bull body weight was measured every 3 week, and body condition score and scrotal circumference were recorded on weeks 0, 9 and 18. Semen and serum were collected every 3 weeks for sperm evaluation and testosterone measurement, respectively. There was a treatment × week interaction (p < 0.01) for sperm with primary defects; bulls in CONT group had a greater (p = 0.01) percentage of sperm with primary defects than bulls treated with insecticides at week 18. Overall and progressive sperm motility, normal sperm morphology, secondary sperm defects and serum testosterone concentrations changed (p < 0.01) over time in all bulls; however, treatment did not affect (p ≥ 0.13) any of these parameters. There were also no treatment effects (p ≥ 0.08) on bull body weight, body condition score or scrotal circumference. The use of pyrethrin‐ and cyfluthrin‐based insecticides, regardless of application, did not negatively affect reproductive parameters in beef bulls when administered over 18 weeks.  相似文献   

Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro          下载免费PDF全文

FC Destro  I Martin  FDC Landim‐Alvarenga  JCP Ferreira  JL Pate 《Reproduction in domestic animals》2016,51(5):848-852

The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid‐luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO₂, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α (PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4‐secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments.  相似文献   

Alphaherpesvirus‐associated disease in greater bilbies (Macrotis lagotis)          下载免费PDF全文

AS Besier  TJ Mahony  M Crockford  JL Gravel  TF Chapman  MA O'Dea 《Australian veterinary journal》2016,94(6):208-212

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Water,salt and nutrient fluxes of tropical tidal salt flats   总被引：2，自引：0，他引：2  

Ridd  Peter  Sam  Renagi  Hollins  Suzanne  Brunskill  Gregg 《Mangroves and Salt Marshes》1997,1(4):229-238

The water budget of a tropical tidal salt flat in dry tropical Australia has been studied with particular emphasis on estimating the groundwater fluxes. Salt was used as a passive tracer to determine some of these fluxes. Groundwater salt accumulation (or loss) was less than 1 kg/m³/month. Surface water leaving the flat at a higher salt concentration than flood water causes a net outwelling of salt of between 0.4 and 1 kg/m²/month. Evaporation from the salt flat was estimated to be 70 mm/month. Using these measurements and a simple model of the groundwater flows, it is concluded that the groundwater loss is less than 40 mm/month. The accuracy of the salt budget was insufficient to rule out the possibility that the net groundwater motion was upwards. Measurements of Si and PO_{_4} fluxes indicated that the net outwelling due to surface water flows were respectively 3 mmole/m²/month and 0.014 mmole/m²/month. A net groundwater loss of 10 mm would produce a similar magnitude outwelling due to groundwater discharge. The mangrove-fringed tropical tidal salt flats are the source of large quantities of nutrients that are released to the near-shore zone.  相似文献