Attaching and effacing lesions in the large intestine of an eight-month-old heifer associated with Escherichia coli O26 infection in a group of animals with dysentery     

Pearson GR  Bazeley KJ  Jones JR  Gunning RF  Green MJ  Cookson A  Woodward MJ 《The Veterinary record》1999,145(13):370-373

Escherichia coli O26:K60, with genetic attributes consistent with a potentially human enterohaemorrhagic E. coli was isolated from the faeces of an eight-month-old heifer with dysentery. Attaching and effacing lesions were identified in the colon of a similarly affected heifer examined postmortem, and shown to be associated with E. coli O26 by specific immunolabelling.  相似文献   

Production and functional characterization of recombinant bovine interleukin-8 as a specific neutrophil activator and chemoattractant     

Caswell JL  Middleton DM  Gordon JR 《Veterinary immunology and immunopathology》1999,67(4):327-340

Interleukin-8, a member of the chemokine family of cytokines, is a potent neutrophil chemoattractant in many non-rodent species. In this study, recombinant bovine interleukin-8 (rbIL-8) was expressed in bacteria as a glutathione-S-transferase fusion protein. The fusion protein was purified by glutathione-Sepharose affinity chromatography and recombinant rbIL-8 was eluted by cleaving with thrombin. The purified rbIL-8 molecule was approximately 8 kDa and was confirmed as authentic IL-8 by Western analysis. Recombinant bovine IL-8 induced specific dose-dependent in vitro chemotaxis of neutrophils at doses as low as 1.0 ng/ml, and this activity was inhibited by pre-treatment of rbIL-8 with a monoclonal antibody to ovine IL-8. Neutrophils exposed to rbIL-8 developed pseudopodia and became elongated as determined by microscopic analysis and flow cytometry. Injection of 3.3 ng to 3.3 microg of rbIL-8 into the skin of a normal calf induced dose-dependent recruitment of neutrophils but not eosinophils. Intravascular margination of neutrophils was obvious at the injection sites from 15 to 60 min after administration of rbIL-8, and extravascular neutrophil numbers increased steadily from 1 to 18 h after injection. Neutrophils with morphologic features of apoptosis were detected in these lesions at 18 and 30 h after injection, and this correlated with reduction in the number of dermal neutrophils. These results confirm unequivocally that bovine IL-8 functions as a neutrophil, but not an eosinophil, chemoattractant in vitro and in vivo.  相似文献   

Uranium and Lead Isotopic Stability in a Metamict Zircon under Experimental Hydrothermal Conditions     

Pidgeon RT  O'neil JR  Silver LT 《Science (New York, N.Y.)》1966,154(3756):1538-1540

Abstract. Disturbance of the uranium-lead isotopic system in a metamict Ceylon zircon has been produced in a 2 molal NaCI solution at 500 degrees C and 1000 bars fluid pressure. Loss of radiogenic lead to the extent of 61 percent in 13 days was the most significant effect. The experimental results support the episodic rather than continuous lead-loss interpretation of natural zircon systems utilized in geochronology.  相似文献   

Population policies in Appalachia: investment strategies and the problem of outmigration     

Alexander JR 《Growth and change》1978,9(3):14-21

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Hydrogen Evolution by Nitrogen-Fixing Anabaena cylindrica Cultures     

Benemann JR  Weare NM 《Science (New York, N.Y.)》1974,184(4133):174-175

Actively growing, nitrogen-fixing cultures of the blue-green alga Anabaena cylindrica can simultaneously evolve hydrogen and oxygen frolni water and light. Hydrogen evolution was strongly inhibited by N(2) but only slightly by CO or O(2), characteristics of the nitrogenase reaction in the heterocysts of Anabaena cylindrica. We suggest that this reaction has potential use in solar energy conversion.  相似文献   

Polyvinylpyrrolidone (HPX): a new treatment for bovine mastitis     

McAULIFF JL  PHILLIPS WV  STEELE JR 《Journal of the American Veterinary Medical Association》1958,133(3):169-171

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Distribution of allergic and blocking activity in human serum proteins fractionated by electrophoresis convection     

LOVELESS MH  CANN JR 《Science (New York, N.Y.)》1953,117(3031):105-108

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It's all in the mix: infection transmission in populations     

Wood JL  Newton JR  Daly J  Park AW  Mumford JA 《Equine veterinary journal》2003,35(6):526-528

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Insect chemical ecology research in the United States Department of Agriculture-Agricultural Research Service     

Aldrich JR  Bartelt RJ  Dickens JC  Knight AL  Light DM  Tumlinson JH 《Pest management science》2003,59(6-7):777-787

This multi-author paper reviews current work by USDA-ARS scientists in the field of chemical ecology. Work with pheromones, the discovery and development of the codling moth kairomone, studies on insect-plant interactions and chemically mediated tritrophic plant-insect interactions have led to practical methods for control of important insect pests.  相似文献   

Taurine deficiency in dogs with dilated cardiomyopathy: 12 cases (1997-2001)     

Fascetti AJ  Reed JR  Rogers QR  Backus RC 《Journal of the American Veterinary Medical Association》2003,223(8):1137-1141

OBJECTIVE: To determine signalment, history, clinical signs, blood and plasma taurine concentrations, electrocardiographic and echocardiographic findings, treatment, and outcome of dogs with low blood or plasma taurine concentrations and dilated cardiomyopathy (DCM). DESIGN: Retrospective study. ANIMALS: 12 client-owned dogs with low blood or plasma taurine concentrations and DCM. PROCEDURE: Medical records were reviewed, and clinical data were obtained. RESULTS: All 12 dogs were being fed a commercial dry diet containing lamb meal, rice, or both as primary ingredients. Cardiac function and plasma taurine concentration improved with treatment and taurine supplementation. Seven of the 12 dogs that were still alive at the time of the study were receiving no cardiac medications except taurine. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that consumption of certain commercial diets may be associated with low blood or plasma taurine concentrations and DCM in dogs. Taurine supplementation may result in prolonged survival times in these dogs, which is not typical for dogs with DCM. Samples should be submitted for measurement of blood and plasma taurine concentrations in dogs with DCM, and taurine supplementation is recommended while results of these analyses are pending.  相似文献