Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy     

Pigatto JA  Andrade MC  Laus JL  Santos JM  Brooks DE  Guedes PM  Barros PS 《Veterinary ophthalmology》2004,7(3):205-208

The objective of this study was to examine the endothelial surface morphology and to perform morphometric analysis of the corneal endothelial cells of Yacare caiman (Caiman yacare) using scanning electron microscopy. Morphometric analysis with regard to polygonality, mean cell area, cell density and coefficient of variation of mean cell area was performed. Cell areas were measured using image analysis software. The normal corneal endothelium of Yacare caiman consisted of polygonal cells of uniform size and shape with interdigitations of the cell borders. Microvilli appeared as protrusions on the cellular surface. The average cell area was 270 +/- 24 microm(2) and the endothelial cell density was 3704 +/- 324 cells/mm(2). The coefficient of variation of cell area was 0.22. This study demonstrates that the Yacare caiman corneal endothelium is similar to those described in other vertebrates.  相似文献   

Bacterial pseudomycetoma in dwarf hamster, Phodopus sungorus     

Martorell J  Gallifa N  Fondevila D  Rabanal RM 《Veterinary dermatology》2006,17(6):449-452

A case of a dwarf hamster with two progressively growing nodules on the right fore limb is described. These were excised following ineffective medical treatment and were submitted for histopathological examination, which revealed bacterial pseudomycetoma in both nodules. To the authors' knowledge this is the first reported case of bacterial pseudomycetoma in a dwarf hamster.  相似文献   

Multifocal cutaneous histiocytic sarcoma in a young dog and review of histiocytic cell immunophenotyping     

Mastrorilli C  Spangler EA  Christopherson PW  Aubry OA  Newton JC  Smith AN  Kennis RA  Weismann JL  Moore PF 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(3):412-418

A 9‐month‐old male Great Dane had progressive generalized nodular dermatopathy for several months. There were > 100 raised, alopecic, firm, painful nodules throughout the skin. Aspirates from several lesions yielded moderate numbers of irregularly round or polygonal to spindle‐shaped cells with mild to moderate anisocytosis and few inflammatory cells, and the cytologic interpretation was proliferation of mesenchymal or histiocytic cells. On histopathologic examination, nodules were composed of densely packed sheets of round to spindle‐shaped cells with mild anisokaryosis and low mitotic activity. Multifocal histiocytic sarcoma with a spindle‐cell pattern was diagnosed based on morphologic features and intense expression of CD18. Additional immunophenotypic analysis on frozen sections of tissue confirmed the diagnosis of histiocytic sarcoma; expression of CD18, CD45, CD1a, CD11b, and CD11c, limited expression of Thy‐1 (CD90) and CD80, and lack of expression of CD4, CD11d, and CD86 indicated that the cells were likely interstitial dendritic cells; a review of reactive and neoplastic dendritic cells is provided. Based on staging, internal organs were not affected. Sequential treatment with lomustine and doxorubicin failed to prevent progression of the cutaneous lesions, and the dog died 3 months after initial diagnosis. At necropsy, a focus of neoplastic cells was present in one lymph node, but except for skin other organs were not involved. The clinical presentation of histiocytic sarcoma may be unusual, and neoplastic cells may lack overt features of malignancy on cytologic and histopathologic examination. In some instances, immunophenotyping is required to differentiate histiocytic sarcoma from other histiocytic disorders.  相似文献   

Deep planting with shelters improves performance of different stocktype sizes under arid Mediterranean conditions     

Oliet  Juan A.  Artero  Francisco  Cuadros  Simón  Puértolas  Jaime  Luna  Lourdes  Grau  Jose M. 《New Forests》2012,43(5-6):925-939

New Forests - Increasing plant size has been considered a way to improve survival and growth of planted containerized stock under dry conditions. Additionally, deep planting provides advantages to...  相似文献   

A cadaver study comparing two approaches for performing maxillary nerve block in dogs     

Jaime Viscasillas  Christopher J Seymour  David C Brodbelt 《Veterinary anaesthesia and analgesia》2013,40(2):212-219

Objective To compare the success by inexperienced anaesthetists of using a modified infraorbital approach to the maxillary nerve with the traditional percutaneous approach. Study design Prospective, randomized, blinded controlled study. Animals Heads from 37 euthanized Beagle and Beagle cross dogs. Methods Four anaesthetists were recruited to perform two different approaches to block the maxillary nerve of the cadavers. The infraorbital (I) approach advanced an intravenous catheter along the infraorbital canal. Earlier measurements from scans of similar heads were used to assess suitable catheter size. The percutaneous (P) approach introduced a needle percutaneously just below the ventral border of the zygomatic arch. The side of the head where the technique was to be performed was randomized. A total volume of 0.5 mL methylene blue was injected in each approach. After completion of injections, head dissections were performed by an investigator unaware of the approach used and staining of the maxillary and pterygopalatine nerves was evaluated. Chi squared analysis examined the relationship between the methods (p < 0.05). Complications related to the techniques, such as intravascular/intraneural injection and location of the dye, were evaluated macroscopically. Results Maxillary nerve staining >6 mm was found in 64.9% (I) versus 21.6% (P) attempts; staining <6 mm was found in 27% (I) versus 21.6% (P); and no nerve staining 8.1% (I) versus 56.8% (M). Pterygopalatine nerve staining was found in 70% (I) versus 21% (P). The infraorbital approach demonstrated significantly higher maxillary and pterygopalatine nerve staining compared to the percutaneous approach (p = 0.001 for both nerves). No evidence of intravascular/intraneural injections was found. Conclusion and clinical relevance The infraorbital approach was more successful than the percutaneous approach when performed by inexperienced anaesthetists. No macroscopic complications were observed.  相似文献   

Transient Horner′s syndrome after paravertebral brachial plexus blockade in a dog     

Jaime Viscasillas  Sandra Sanchis‐Mora  Carol Hoy  Hatim Alibhai 《Veterinary anaesthesia and analgesia》2013,40(1):104-106

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Live weight, carcass ultrasound images, and visual scores in Angus cattle under feeding regimes in Brazil     

Luís Fernando Batista Pinto  Jaime Urdapilleta Tarouco  Victor Breno Pedrosa  Adriana de Farias Jucá  André Gustavo Leão  Antonia Kécya França Moita 《Tropical animal health and production》2013,45(6):1281-1287

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Effect of Incorporating White,Red or Black Quinoa Flours on Free and Bound Polyphenol Content,Antioxidant Activity and Colour of Bread     

Ballester-Sánchez  Jaime  Gil  Jose Vicente  Haros  Claudia Monika  Fernández-Espinar  María Teresa 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(2):185-191

Plant Foods for Human Nutrition - Interest in quinoa as a functional food ingredient is currently emerging. The flours from white, red and black quinoa seeds were analysed in terms of total...  相似文献   

Thinning enhances stool resistance to an extreme drought in a Mediterranean Quercus ilex L. coppice: insights for adaptation     

Domingo  Jose  Zavala  Miguel A.  Madrigal-González  Jaime 《New Forests》2020,51(4):597-613

New Forests - Mediterranean holm oak forests are subjected to chronic seasonal droughts coinciding with the warmest conditions during the summer. Importantly, climate change projections support...  相似文献   

Oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of Atlantic salmon     

Navarrete P  Mardones P  Opazo R  Espejo R  Romero J 《Journal of aquatic animal health》2008,20(3):177-183

The effect of oxytetracycline (OTC) treatment on intestinal bacterial populations in juvenile Atlantic salmon Salmo salar was evaluated. Oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. Restriction fragment length polymorphism and sequencing of 16S rDNA from isolates were used to analyze the intestinal microbiota before, during, and after OTC administration. The microbiota from untreated fish was more diverse, consisting mainly of Pseudomonas, Acinetobacter, Bacillus, Flavobacterium, Psycrobacter, and Brevundimonas spp. In contrast, the microbiota of the OTC-treated group was characterized by lower diversity and consisted only of Aeromonas, clustering with A. sobria and A. salmonicida. Antibiotic-resistant isolates were identified as Aeromonas spp.; sequencing the resistance determinant showed it to be the tetE gene. Overall, OTC treatment changed the composition of the intestinal microbiota of Atlantic salmon, as evidenced by a reduction in bacterial diversity. These results support the current concern that antibiotic treatment can facilitate the proliferation of opportunistic bacteria by eradicating competing microorganisms.  相似文献