猪带绦虫六钩蚴cDNA文库的构建与筛选   总被引：1，自引：0，他引：1  

唐雨德  陆承平 《中国兽医学报》2003,23(6):567-569

在体外将猪带绦虫虫卵孵化为有活力的六钩蚴。采用商品化试剂盒抽提六钩蚴总 RNA、m RNA,反转录为双链c DNA,再加 Eco R Adapter。在去除小片段后 ,dsc DNA与 λgt11噬菌体 DNA连接 ,经蛋白包装 ,构建了猪带绦虫六钩蚴λgt11c DNA文库。该文库效价为 3.5× 10 9pfu/ m L ,重组 DNA片段大小为 0 .5～ 3.2 kb,平均为 1.6 kb,蓝白斑比 1∶ 9。用抗体探针对文库进行免疫学筛选 ,在消除非特异性反应的基础上筛选约 10 6 重组子 ,共得到 118株强阳性克隆 ;应用 PCR鉴定上述部分阳性克隆 ,均扩增到 0 .5 kb以上的片段。结果显示 ,构建的文库合格 ,含六钩蚴所有抗原基因 ,可用于六钩蚴 c DNA克隆的筛选 ;用免疫学与 PCR联合筛选 c DNA文库 ,可消除假阳性  相似文献   

Leishmaniasis phytotherapy. Nature's leadership against an ancient disease.     

P B de Carvalho  E I Ferreira 《Fitoterapia》2001,72(6):599-618

The use of phytotherapy to treat human diseases has its roots in pre-historical times. Despite the modern advances achieved in the field of synthetic chemistry, the most efficient drugs available have their genesis directly or indirectly related with the vegetal kingdom. Indigenous communities have long used plant extracts to treat illnesses. Many of these extracts have shown effective action, with new bioactive compounds being extracted and screened every year. These extracts have also proven to be good sources of therapeutic agents to the treatment of Leishmaniasis. This work highlights some of these agents, while trying to emphasize the importance of plants as a source of new and powerful drugs against this widespread disease.  相似文献   

A Preliminary Trial of Detomidine/Opiate Combination in the Horse     

B.S. Paton  K.W. Clarke 《Veterinary anaesthesia and analgesia》1986,14(1):44-52

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Genetic diversity of herbicide-resistant and -susceptible Avena fatua populations in North Dakota and Minnesota     

L W MENGISTU  C G MESSERSMITH  & M J CHRISTOFFERS 《Weed Research》2005,45(6):413-423

Genetic diversity within and among 20 herbicide-resistant (HR) and 16 herbicide-susceptible (HS) Avena fatua multi-field populations was determined using 82 polymorphic loci resulting from two intersimple sequence repeat (ISSR) primers and one long-primer random amplified polymorphic DNA (LP-RAPD) primer. Collections from the Red River Valley of North Dakota and Minnesota, sampled in 1964 and 2000, represented A. fatua populations before and after intensive exposure to herbicides. A 1995 collection from south-west North Dakota represented A. fatua exposed to low herbicide selection. Despite differences in years of herbicide exposure among collections, both HR and HS populations from every collection maintained nearly similar levels of ISSR and RAPD diversity. Genetic differentiation among populations (G_ST) varied from 11% to 13% among HR populations and from 9% to 16% among HS populations, indicating that 84–91% of total variation remained within HS or within HR populations. Minimal difference in gene diversity between HR and HS is consistent with multiple origins of resistance, where HR A. fatua most likely evolved from diverse founding individuals.  相似文献   

Medullary thyroid carcinoma in a horse     

M A van der Velden  H Meulenaar 《Veterinary pathology》1986,23(5):622-624

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Canine distal renal tubular acidosis and urolithiasis   总被引：1，自引：0，他引：1  

D J Polzin  C A Osborne  F W Bell 《Veterinary Clinics of North America: Small Animal Practice》1986,16(2):241-250

Distal RTA is characterized by decreased distal renal tubular hydrogen ion secretion, decreased ability to acidify urine, hypercalciuria, hyperphosphaturia, hypocitraturia, and metabolic acidosis. Because of the resulting alterations in urine composition and pH, patients with distal RTA are predisposed to urolithiasis and renal calcification. Diagnosis of distal RTA is important because it is a potentially reversible disorder that, left untreated, may cause nephrocalcinosis, recurrent urolith formation, moderate to severe metabolic acidosis, and renal failure.  相似文献   

Coxofemoral Luxation in the Dog and Cat     

ANTHONY W. P. BASHER BVetMed  mrcvs  MARY C. WALTER dvm  CHARLES D. NEWTON dvm  ms  Diplomateacvs 《Veterinary surgery : VS》1986,15(5):356-362

The medical records from 95 cases of coxofemoral luxation in dogs and cats were reviewed. Unilateral craniodorsal luxation was most common, (78.1% of dogs, 72.7% of cats). Trauma from being struck by an automobile was the most frequent cause (59.0%). Treatment was closed (manual) or open (surgical) reduction. The failure rate following single closed reduction was 64.8%. The surgical reduction procedure included capsular repair (capsulorrhaphy) via a craniolateral approach to the hip or via trochanteric osteotomy or gluteal tenotomy. The success rate following reduction via the craniolateral approach was 82%; no failure was recorded with trochanteric transposition, but reluxa-tion followed the single gluteal tenotomy. The Ehmer sling was the most common external fixation and was kept in place an average of 12.5 days. Thirty-three animals were available for follow-up, and no difference was recorded in limb function between the two groups of animals treated by closed vs surgical reduction. Patients with degenerative joint disease at the time of luxation were less likely to be successfully reduced; 64.3% of these animals were eventually treated by femoral head and neck excision. Thirty-eight patients had associated major injuries. Long-term follow-up results were better in those patients with concomitant orthopedic injuries than in those without other injuries.  相似文献   

Effects of low and high calcium intake prepartum on calcium mobilization rate around parturition in dairy cows     

A E van de Braak  A T van 't Klooster  A Malestein  J A Faber 《The Veterinary quarterly》1986,8(1):12-23

Forty-one dairy cows were fed a low (LCa-13 g/d) and a high (HCa-83.5 g/d) calcium ration in the 8 weeks prior to parturition and the effect on the Ca mobilization rate around parturition was studied. Plasma Ca values were stable in the LCa group around parturition. In the older cows of the HCa group a very slight decrease in the mean plasma Ca was observed: 2.58 mmol/l at 12-36 h ante partum decreased to 2.38 mmol/l at parturition. Hypocalcaemia, which commonly occurs around parturition, did not occur in 40 of the cows. A subclinical hypocalcaemia (1.8 mmol/l) occurred in one cow (parity 10) from the HCa group. To assess the efficiency of Ca mobilization, a severe hypocalcaemia (1.0 mmol/l) with clinical signs was induced by means of Na2EDTA infusion (0.90 mmol/min), starting at 10 h post-partum. The older cows in the LCa group required more Na2EDTA than those in the HCa group. Higher urinary hydroxyproline excretion in the week before parturition in the LCa than in the HCa group suggested a higher bone turnover. Plasma PTH levels around parturition were not significantly different between the groups. The amount of colostrum milked out in the first 10 h post-partum did not influence Ca homeostasis around parturition. The results contradict those of many other experiments in which hypocalcaemia was observed in cows ingesting high levels of Ca. It is concluded that the restricted feed intake prepartum possibly had a favourable effect on Ca homeostasis.  相似文献   

A remarkable new type of mollusks     

Dall WH 《Science (New York, N.Y.)》1884,4(76):50-51

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Laws against quacks     

Purrington WA 《Science (New York, N.Y.)》1886,8(200):514-515

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