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41.

Context

Landscape ecology has traditionally been taught through theoretical classes or computer labs. On the other hand, field labs have been generally less used as a way of teaching landscape ecology concepts.

Objectives

We show that field labs with an inquiry-based approach, where students are involved in the investigation, are feasible for training students in landscape ecology. We evaluated how common field labs are in landscape ecology courses, and also their contribution for student learning.

Methods

We evaluated whether field labs are used in landscape ecology courses by scanning available syllabi. We also used outcomes from a course offered in Brazil to show how field labs can be integrated into landscape ecology courses.

Results

Only 18.2 % of the 44 syllabi we found had field labs. The case study developed in Brazil showed that field labs allowed students to develop important skills, including the ability to design field studies, choose appropriate scales of analysis, detect ecological patterns, and judge multiple hypotheses.

Conclusions

Field labs are still uncommon in landscape ecology courses, but they can be a useful tool to teach landscape ecology concepts and to help students developing the necessary skills to do research. We offer recommendations regarding how to incorporate field labs in landscape ecology courses.
  相似文献   
42.
ObjectiveTo compare cardiac output (CO) measured by Doppler echocardiography and thermodilution techniques in spontaneously breathing dogs during continuous infusion of propofol. To do so, CO was obtained using the thermodilution method (COTD) and Doppler evaluation of pulmonary flow (CODP) and aortic flow (CODA).Study designProspective cohort study.AnimalsEight adult dogs weighing 8.3 ± 2.0 kg.MethodsPropofol was used for induction (7.5 ± 1.9 mg kg?1 IV) followed by a continuous rate infusion at 0.7 mg kg?1 minute?1. The animals were positioned in left lateral recumbency on an echocardiography table that allowed for positioning of the transducer at the 3rd and 5th intercostal spaces of the left hemithorax for Doppler evaluation of pulmonary and aortic valves, respectively. CODP and CODA were calculated from pulmonary and aortic velocity spectra, respectively. A pulmonary artery catheter was inserted via the jugular vein and positioned inside the lumen of the pulmonary artery in order to evaluate COTD. The first measurement of COTD, CODP and CODA was performed 30 minutes after beginning continuous infusion (T0) and then at 15‐minute intervals (T15, T30, T45 and T60). Numeric data were submitted to two‐way anova for repeated measurements, Pearson’s correlation coefficient and Bland &; Altman analysis. Data are presented as mean ± SD.ResultsAt T0, COTD was lower than CODA. CODA was higher than COTD and CODP at T30, T45 and T60. The difference between the COTD and CODP, when all data were included, was ?0.04 ± 0.22 L minute?1 and Pearson’s correlation coefficient (r) was 0.86. The difference between the COTD and CODA was ?0.87 ± 0.54 L minute?1 and r = 0.69. For COTD and CODP, the difference was ?0.82 ± 0.59 L minute?1 and r = 0.61.ConclusionDoppler evaluation of pulmonary flow was a clinically acceptable method for assessing the CO in propofol‐anesthetized dogs.  相似文献   
43.
The objective of this work was to evaluate the semen characteristics of C. macropomum fish from three successive semen sample collections taken during the same reproductive cycle. Six breeders (6.4 ± 1.5 kg) induced with crude carp pituitary extract (2.5 mg kg?1) were used. Samples of semen were collected in graduated syringes after a period of 260 degree‐hours. The semen was collected from the same males in September 2014 (sample 1), December 2014 (64 days after the first collection – sample 2) and February 2015 (86 days after the second collection – sample 3). The semen volume was higher (P < 0.05) in the sample 1 (6.7 mL) and sample 2 (3.4 mL) than in the sample 3 (1.0 mL). Sperm concentration and normal sperm did not significantly differ between samples. Likewise, sperm motility, curvilinear velocity, displacement velocity, oscillation and beat cross frequency did not vary, whereas straight‐line velocity, straightness and progression were higher (P < 0.05) in sample 2 (December). In general, despite lower amounts, the semen appeared to be of good quality for all three samples collected during the same reproductive period.  相似文献   
44.
Re‐ensiling of previously ensiled forage has been a common practice in Brazil, and the use of inoculants may provide a means of reducing dry‐matter (DM) loss. This study aimed to determine the effect of re‐ensiling and the use of microbial inoculants on the quality of sorghum silage. Treatments were presence/absence of an inoculant (Lactobacillus plantarum and Propionibacterium acidipropionici) in the silage, and the re‐ensiling, or not, of the material after 24 h of exposure to air, and these were tested in a factorial 2 × 2 design. Losses due to gas, effluent and total DM were assessed, as were the fermentation characteristics, chemical composition, aerobic stability, and aerobic counts of microorganisms. Effluent loss was higher in re‐ensiled silage, and these silages had lower lactic acid content and higher levels of acetic and propionic acids. The in vitro DM digestibility was lower in the re‐ensiled sorghum silages. The re‐ensiled silage had higher aerobic stability. The inoculant only increased the acetic acid content of the silage. The re‐ensiling of sorghum silage increased effluent loss by 71·2%, and reduced DM digestibility by 5·35%. The use of inoculant did not influence the quality of sorghum silage.  相似文献   
45.
Landscape Ecology - Habitat fragmentation per se (habitat subdivision independent of habitat loss) is a major driver of biodiversity change, potentially due to its impacts on climate. Habitat...  相似文献   
46.
Objective To study the echocardiographic effects of isoflurane at an end‐tidal concentration approximating 1.0 times the minimum alveolar concentration (MAC) in healthy unpremedicated dogs. Study design Prospective experimental trial. Animals Sixteen mature mongrel dogs of either sex weighing 11.06 ± 2.72 kg. Methods After performing a baseline echocardiogram in the awake animal, anesthesia was induced with increasing inspired concentrations of isoflurane via a face mask until tracheal intubation was possible. Following intubation, the end‐tidal concentration was decreased to 1.4% for the rest of the anesthetic period. Serial echocardiograms were recorded at 25, 40, and 55 minutes after the end‐tidal concentration was reached. Results No changes were observed in heart rate. However, significant decreases were seen in left ventricular end‐diastolic diameter (Mean maximal change: 13.8%), interventricular septal thickness during systole (15.2%), interventricular septal thickening fraction (72.2%), left ventricular free wall thickening fraction (63.5%), ejection fraction (39.9%), and fractional shortening (46.7%). In addition, peak flow velocities across mitral, pulmonic, and aortic valves were significantly lower than baseline values. Decreases were also observed in end‐diastolic left ventricular volume index (approximately 32.1% from the awake value), stroke index (58.2%), and cardiac index (55.3%) when compared with awake measurements. Conclusions and clinical relevance Our results indicate that 1 × MAC isoflurane caused significant myocardial depression in healthy dogs. These changes in myocardial function need to be considered carefully when isoflurane is to be used in dogs with poor cardiovascular reserve.  相似文献   
47.

Context

Native vegetation is often used as a proxy for habitat to estimate habitat availability in landscapes. This approach may lead to incorrect estimates of the impacts of habitat loss and fragmentation on species, which have not been thoroughly quantified so far.

Objectives

We quantified to what extent the loss of native vegetation reflect actual habitat loss by native species in landscapes. We tested the hypothesis that habitat availability declines at greater rates than native vegetation and thus is overestimated when it is quantified on the basis of native vegetation.

Methods

Using simulations, we quantified how the loss of native vegetation in artificial and real landscapes affects habitat availability for species with different habitat requirements. We contrasted a generalist species, which uses all native vegetation, with 10 habitat-specialist species classified into three categories (interior, patchy and riparian species).

Results

Habitat availability generally declined at greater rates than native vegetation for all specialist species. This pattern was apparent for different specialist species in a broad range of landscape types. Interior species always lost habitat availability more rapidly than the generalist species. Most riparian species lost habitat availability more rapidly than the generalist species. Responses of patchy species were more complex, depending on their dispersal abilities and landscape structure.

Conclusions

Habitat availability is likely to be overestimated when native vegetation is used as proxy for habitat, because habitat availability will generally decline at greater rates than native vegetation. Therefore, a species-centered approach should be adopted when estimating habitat availability in landscapes.
  相似文献   
48.
49.
Recently, the Tei-index, a noninvasive index that combines systolic and diastolic time intervals, has been proposed to assess global cardiac performance. However, the effects of isoflurane on the Tei-index have not been characterized. This study aimed at studying the effects of 1.0 minimal alveolar concentration isoflurane anesthesia on the pre-ejection period (PEP), left ventricular ejection time (LVET), PEP/LVET ratio, isovolumic relaxation time (IVRT), stroke index (SI), cardiac index (CI), heart rate (HR), and the Tei-index in healthy unpremedicated dogs. We observed significant increases in PEP, PEP/LVET ratio, IVRT, and TEI, whose maximal increases obtained throughout the study were 47%, 48%, 78%, and 56%, respectively. The LVET and HR did not change significantly, whereas the SI and CI decreased during anesthesia (29% and 26%, respectively). In conclusion, isoflurane produced direct effects on the Tei-index. The changes in systolic and diastolic parameters were supportive of this finding and were consistent with an overall impairment of left ventricular function during anesthesia.  相似文献   
50.
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