Neuroinflammatory diseases of the central nervous system of dogs: A retrospective study of 207 cases (2008–2019)     

Jessica A. Elbert  Wilson Yau  Daniel R. Rissi 《The Canadian veterinary journal. La revue veterinaire canadienne》2022,63(2):178

In this study we describe 207 cases of neuroinflammatory diseases of the central nervous system (CNS) in dogs autopsied at the Athens Veterinary Diagnostic Laboratory (University of Georgia, United States) from 2008 to 2019. Idiopathic and infectious diseases were diagnosed in 111 cases (53.6%) and 96 cases (46.4%), respectively. Idiopathic diseases consisted of granulomatous meningoencephalomyelitis (n = 42; 37.8% of idiopathic cases), nonspecific lymphoplasmacytic meningoencephalomyelitis (n = 39; 35.1%), necrotizing meningoencephalomyelitis (n = 22; 19.8%), presumed steroid-responsive meningitis-arteritis (n = 6; 5.4%), and necrotizing leukoencephalitis (n = 2; 1.8%). Infectious diseases consisted of bacterial infections (n = 49; 51% of infectious cases), viral infections (n = 39; 40.6%), fungal infections (n = 5; 5.2%), and parasitic infections (n = 3; 3.1%). Our study provides an overview of the most frequent neuroinflammatory diseases of the CNS of dogs in our diagnostic routine; indicates that a comprehensive diagnostic approach, including a thorough evaluation of the pathology findings and ancillary laboratory testing results, is important for an adequate diagnosis of neurologic diseases in dogs; and underscores the problems associated with the variability in tissue sample collection methods among cases. The great number of nonspecific lymphoplasmacytic meningoencephalitis also highlights the need for development of molecular laboratory tests to identify potential infectious agents in these cases.  相似文献   

Maize Endosperm Proteins That Contribute to Endosperm Lysine Content     

J. C. Yau  A. J. Bockholt  J. D. Smith  L. W. Rooney  R. D. Waniska 《Cereal Chemistry》1999,76(5):668-672

Nonzein proteins were analyzed using SDS-PAGE to study their relationship to lysine level in mature endosperm of three isogenic lines and 29 inbred lines. The majority of nonzein proteins are glutelins that contain a relatively high lysine content. Trinitrobenzene-sulfonic acid was used to visualize high-lysine proteins on nitrocellulose membrane. Seven intense yellow bands (high-lysine proteins) were found in the glutelin fraction: 35, 43, 48, 52, 84, 92, and 100 kDa. These seven glutelin proteins were also significantly correlated to each other. Lysine content of the triads correlated with three glutelin proteins (35, 43, and 52 kDa), while lysine content of 29 inbred lines correlated with six glutelin proteins (all except 52-kDa protein). Immunoassays based on these higher lysine glutelin proteins provide an effective screening method in the presence of variable zein levels. Retention or improvement of lysine content is possible using this information in a maize improvement program.  相似文献