Water requirements of canine athletes during multi‐day exercise          下载免费PDF全文

Lara Stephens‐Brown  Michael Davis 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):1149-1154

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Serologic and fecal markers to predict response to induction therapy in dogs with idiopathic inflammatory bowel disease          下载免费PDF全文

Cristiane C. Otoni  Romy M. Heilmann  Mercedes García‐Sancho  Angel Sainz  Mark R. Ackermann  Jan S. Suchodolski  Jörg M. Steiner  Albert E. Jergens 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):999-1008

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Immunohistochemical differentiation of reactive from malignant mesothelium as a diagnostic aid in canine pericardial disease          下载免费PDF全文

E. Milne  Y. Martinez Pereira  C. Muir  T. Scase  D. J. Shaw  G. McGregor  L. Oldroyd  E. Scurrell  M. Martin  C. Devine  H. Hodgkiss‐Geere 《The Journal of small animal practice》2018,59(5):261-271

Objectives

To develop a provisional immunohistochemistry panel for distinguishing reactive pericardium, atypical mesothelial proliferation and mesothelioma in dogs.

Materials and Methods

Archived pericardial biopsies were subject to haematoxylin and eosin staining, immunohistochemistry for cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Samples were scored for intensity and number of cells stained.

Results

Ten biopsies of reactive mesothelium, 17 of atypical mesothelial proliferation, 26 of mesothelioma and five of normal pericardium were identified on the basis of haematoxylin and eosin staining. Cytokeratin and vimentin were expressed in all biopsies, confirming mesothelial origin. Normal pericardial samples had the lowest scores for insulin‐like growth factor II mRNA‐binding protein 3, glucose transporter 1 and desmin. Mesothelioma and atypical proliferative samples were similar to each other, with higher scores for insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 than the reactive samples. Desmin staining was variable. Insulin‐like growth factor II mRNA‐binding protein 3 was the best to distinguish between disease groups.

Clinical Significance

An immunohistochemistry panel of cytokeratin, vimentin, insulin‐like growth factor II mRNA‐binding protein 3 and glucose transporter 1 could provide superior information compared with haematoxylin and eosin staining alone in the diagnosis of cases of mesothelial proliferation in canine pericardium, but further validation is warranted.  相似文献   

Shortening of an existing generic online health‐related quality of life instrument for dogs          下载免费PDF全文

J. Reid  L. Wiseman‐Orr  M. Scott 《The Journal of small animal practice》2018,59(6):334-342

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The influence of rapid growth on sodium salicylate pharmacokinetics in male turkeys          下载免费PDF全文

B. Poźniak  K. Motykiewicz‐Pers  T. Grabowski  M. Świtała 《Journal of veterinary pharmacology and therapeutics》2018,41(1):163-165

The aim of this study was to assess the influence of growth on the pharmacokinetics of sodium salicylate (SS) in male turkeys. SS was administered intravenously at a dose of 50 mg/kg. Plasma drug concentrations were assessed by high‐performance liquid chromatography, and pharmacokinetic parameters were calculated by noncompartmental analysis. As the age increased from 6 to 13 weeks (body weight increase from 2.35 to 9.43 kg), median body clearance decreased from 1.34 to 0.87 ml/min/kg. This caused a significant increase in the median mean residence time from 3.42 to 4.44 hr. Elimination phase proved to be biphasic and two elimination half‐lives (T_1/2el) were distinguished. Whereas T_1/2el1 was found to increase with age by 128%, T_1/2el2 represented a later but faster and less age‐dependent phase of elimination (increase by 56% in the respective groups). Volume of distribution decreased with age. These effects may lead to different therapeutic response to SS in turkeys of different age and body weights.  相似文献   

Effects of three-dimensional spheroid culture on equine mesenchymal stem cell plasticity     

Mi Jeong Park  Jienny Lee  Jeong Su Byeon  Da-Un Jeong  Na-Yeon Gu  In-Soo Cho  Sang-Ho Cha 《Veterinary research communications》2018,42(3):171-181

Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was performed to analyze gene expression. As results, expression levels of pluripotent markers were increased depending on spheroid size and the production of PGE₂ was increased in spheroid formation compared to that in monolayer. Ki-67 showed a remarkable increase in the spheroid formed with 2.0?×?10⁵ cells/drop as compared to that in the monolayer. Expression levels of angiogenesis-inducing factors such as VEGF, IL-6, IL-8, and IL-18 were significantly increased in spheroid formation compared to those in the monolayer. Expression levels of bone morphogenesis-inducing factors such as Cox-2 and TGF-β1 were also significantly increased in spheroid formation compared to those in the monolayer. Expression levels of osteocyte-specific markers such as RUNX2, osteocalcin, and differentiation potential were also significantly increased in spheroid formation compared to those in the monolayer. Therefore, spheroid formation of eAD-MSCs through the hanging drop method can increases the expression of angiogenesis-inducing and bone morphogenesis-inducing factors under optimal culture conditions.  相似文献   

Modification of the metal endoprosthesis limb‐salvage procedure for excision of a large distal radial osteosarcoma in a dog: A case report          下载免费PDF全文

Brandan G. Wustefeld‐Janssens BVSc  Diplomate ECVS  Mary Lafferty CVT  Bernard Séguin DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》2018,47(6):802-808

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Incidence and risk factors for neurological signs after attenuation of single congenital portosystemic shunts in 253 dogs          下载免费PDF全文

Rhiannon Strickland BVetMed  Michael S. Tivers BVSc  PhD  DipECVS  Sophie E. Adamantos BVSc  DACVECC  DECVECC  Tom R. Harcourt‐Brown MA  VetMB  DipECVN  Robert C. Fowkes BSc  PhD  Victoria J. Lipscomb MA  VetMB  DipECVS 《Veterinary surgery : VS》2018,47(6):745-755

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Effects of hyperbaric oxygen therapy on uncomplicated incisional and open wound healing in dogs          下载免费PDF全文

Christian R. Latimer DVM  Cassie N. Lux DVM  DACVS‐SA  Sarah Roberts BS  DVM  Marti G. Drum DVM  PhD  DACVSMR  Cheryl Braswell DVM  DACVECC  Mee Ja M. Sula DVM  DACVP 《Veterinary surgery : VS》2018,47(6):827-836

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Laryngeal mask airway and transient hypercapnic hyperpnea for video‐endoscopic assessment of unilateral laryngeal paralysis in dogs          下载免费PDF全文

Daniel M. Sakai MV  DACVAA  Manuel Martin‐Flores MV  DACVAA  Amanda K. Jones PhD  Galina M. Hayes BVSc  PhD  DACVECC  DACVS‐SA  Marina J. McConkey DVM  DACVS‐SA  Jonathan Cheetham VetMB  PhD  DACVS‐LA 《Veterinary surgery : VS》2018,47(4):543-548

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