Effects of various adjuvants on efficacy of a vaccine against Streptococcus bovis and Lactobacillus spp in cattle     

Shu Q  Hillard MA  Bindon BM  Duan E  Xu Y  Bird SH  Rowe JB  Oddy VH  Gill HS 《American journal of veterinary research》2000,61(7):839-843

OBJECTIVE: To determine efficacy of vaccines incorporating QuilA, alum, dextran combined with mineral oil, or Freund adjuvant for immunization of feedlot cattle against Streptococcus bovis and Lactobacillus spp. ANIMALS: 24 steers housed under feedlot conditions. PROCEDURE: Steers were randomly assigned to 4 experimental groups and a control group. Animals in experimental groups were inoculated on days 0 and 26 with vaccines containing Freund adjuvant (FCA), QuilA, dextran combined with mineral oil (Dex), or alum as adjuvant. Serum anti-S bovis and anti-Lactobacillus IgG concentrations were measured, along with fecal pH, ruminal fluid pH, and number of S bovis and Lactobacillus spp in ruminal fluid. RESULTS: Throughout the study, serum anti-S bovis and anti-Lactobacillus IgG concentrations for animals in the Dex, QuilA, and alum groups were similar to or significantly higher than concentrations for animals in the FCA group. Serum anti-S bovis and anti-Lactobacillus IgG concentrations were significantly increased on days 26 through 75 in all 4 experimental groups, and there was a linear relationship between anti-S bovis and anti-Lactobacillus IgG concentrations. For animals in the QuilA and Dex groups, mean pH of feces throughout the period of experiment were significantly higher and numbers of S bovis and Lactobacillus spp in ruminal fluid on day 47 were significantly lower than values for control cattle. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that immunization of feedlot steers against S bovis and Lactobacillus spp with vaccines incorporating Freund adjuvant, QuilA, dextran, or alum as an adjuvant effectively induced high, long-lasting serum anti-S bovis and anti-Lactobacillus IgG concentrations. Of the adjuvants tested, dextran may be the most effective.  相似文献   

尿素缓释技术的研究进展     

段玉  张拴林 《饲料工业》2006,27(21):51-53

早在1900年左右就有人在反刍动物营养中利用尿素作为蛋白质补充料。尿素分解产生氨和二氧化碳，微生物利用氨作为氮源营养，将其和一定量的碳水化合物合成菌体蛋白，最后被消化分解为氨基酸而被机体吸收利用。但如果微生物分解尿素产生氨的速度超过微生物的利用速度时，不仅影响瘤胃微生物的生长繁殖，而且动物会出现氨中毒。尿素直接应用，其特殊的异味以及释放氨的速度太快，利用率低、危险性大。由于这方面的原因，尿素等非蛋白氮（NPN）在畜牧业上的推广应用受到很大的限制。因此，使用尿素时应有适量的、易分解的碳水化合物。为提高尿素产品的利用率和安全性，最理想的方法是在尿素被采食进入瘤胃后，控制和减缓氨的释放速度，使瘤胃内维持最适的氨浓度．从而防止氨中毒和提高尿素利用率闭。这也就是我们所说的尿素缓释技术。目前．尿素缓释技术主要有以下几种。  相似文献   

咪鲜胺原药中微量2,4,6-三氯苯酚测定方法的研究     

段文胜  干丽君  崔新义 《农药科学与管理》2006,27(12)

建立了高效液相色谱法测定咪鲜胺原药中微量2,4,6-三氯苯酚含量的方法。采用Hypersil BDS C18色谱柱,以甲醇/水(72/28 V/V)为流动相(每500mL流动相中含0.5g四丁基溴化铵),检测波长为296nm,2,4,6-三氯苯酚的质量浓度在0～871.2μg/L范围内线性关系良好,其线性相关系数为0.999 8;加标回收率为99.71%～102.02%,变异系数为1.14%,检出限为0.88μg/L。  相似文献   

新型植物激素与蔬菜作物抗逆性关系研究进展   总被引：4，自引：0，他引：4  

康云艳  郭世荣  段九菊 《中国蔬菜》2007,1(5):39-39

综述了近年来水杨酸、茉莉酸类化合物、油菜素内酯、多胺和5-氨基乙酰丙酸等新型植物激素与蔬菜作物抗逆性关系的研究进展,从盐胁迫、低温胁迫、高温胁迫、低氧胁迫、病害胁迫、虫害胁迫等方面阐述和分析了新型植物激素与蔬菜作物抗逆性的相关性,并指出相关研究的有待深入之处。  相似文献   

7种小蠹虫酯酶同工酶的比较研究   总被引：2，自引：0，他引：2       下载免费PDF全文

梁靓  段立清  尹凤民  杨永生 《植物保护》2007,33(6):70-74

检疫性小蠹虫的鉴定,因其虫体小,种间形态特征难以准确把握,给木材的检疫工作带来较大困难。当遇到幼虫时还需饲养为成虫再进行鉴定,增加了工作难度。应用聚丙烯酰胺凝胶电泳技术对俄罗斯进境木材7种小蠹虫幼虫、成虫的酯酶同工酶进行了比较分析。结果表明7种小蠹虫幼虫酶带数目均比成虫多,酶活性也较成虫强。幼虫和成虫之间有多条迁移率接近的酶带,酶带相似性及聚类分析均证明可以将这些酶带作为种的特征性谱带。研究表明,当形态特征作为鉴定标准较难准确掌握时,可用幼虫或成虫同工酶电泳图谱来鉴定其种类。  相似文献   

Promotion of plant growth by ACC deaminase-producing soil bacteria     

Bernard R. Glick  Zhenyu Cheng  Jennifer Czarny  Jin Duan 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(3):329-339

  相似文献   

栽培防病措施对防治小麦白粉病的重要作用   总被引：3，自引：0，他引：3  

盛宝钦  段霞瑜  周益林 《植物保护学报》1998,25(3):218-222

为了解栽培措施对防治小麦白粉病的作用,通过不同栽培措施对病情产生不同影响的大量田间调查,明确了小麦早播、密播,过量水、肥条件下的旺苗和种植高度感病品种,都会加重小麦秋苗带菌量。短期播种、合理密植、视墒灌水、配方施肥、培育小麦壮苗及避免种植高感品种等均能减轻小麦秋苗带菌量。小麦秋苗带菌量大,则翌春病菌越冬菌源量也大。种植高感品种在田间有发病中心的作用。应把栽培防病措施规范化、科学化、制度化,以充分发  相似文献   

大肠杆菌内毒素多克隆抗体的制备与纯化     

李生  吕娟  胡格  索占伟  段慧琴  穆祥 《勤云标准版测试》2007,(11)

为制备大肠杆菌内毒素多克隆抗体,应用大肠杆菌内毒素作为抗原免疫5周龄家兔,辛酸-硫酸铵法和葡聚糖凝胶层析法分离提纯抗血清,十二烷基磺酸钠-聚丙烯酰胺凝胶(SDS-PAGE)电泳方法鉴定多抗纯度,紫外分光光度计测定抗体蛋白含量,ELISA法检测抗血清效价与交叉反应;大肠杆菌内毒素抗体含量和效价分别为6.95mg/ml和1:12800;成功制备出抗大肠杆菌内毒素多克隆抗体,为大肠杆菌内毒素病诊断试剂盒的研制奠定基础。  相似文献   

NrDNA internal transcribed spacer revealed molecular diversity in strains of red seaweed <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> and genetic insights for commercial breeding     

ZiMin Hu  FuLi Liu  ZhanRu Shao  JianTing Yao  DeLin Duan 《Genetic Resources and Crop Evolution》2010,57(5):791-799

Unraveling the cryptic genetic diversity and selective breeding network in various Porphyra strains is of significance for conservation and utilization of economically important nori crops, for both current and future needs. Here, we used nuclear ribosomal spacer (ITS1) region to investigate the genetic variation and intra-specific relatedness of 59 Porphyra yezoensis Ueda specimens worldwide using phylogenetic and parsimony genealogical approaches. 23 nrDNA ITS1 genotypes were revealed and clustering analysis grouped them into two distinct clades. High genetic diversity was detected in wild P. yezoensis strains from Miyagi and Hokkaido Prefectures in Japan, while the cultivated strains from China and South Korea exhibited relatively higher genetic diversity likewise, which provided crucial genetic insights for future commercial breeding of P. yezoensis on a global scale. In addition, phylogenetic study has revealed the genetic relationship of strains with unknown parentage to those with known parentage, and also ITS1 sequence pattern could be correlated with the geographic origin of P. yezoensis specimens. All these pedigree information generated from this research can be used to select parents for inter-specific or intra-specific selective breeding and cross breeding to maximize the preservation of stock resource and sustainable development of nori industry.  相似文献   

油罐汽车事故分析     

段多寿 《油气储运》1998,17(6):58-59

油罐汽车装卸油品及运输途中，事故时有发生，列举了四个典型故事宁例，并分别对其进行分析。工作人员的疏忽或误操作以及设备故障造成冒油泄漏静电放电，是导致事故发生的主要原因：“转换装油”造成的事故也应当引起足够的重视，总结指出必须使用符合技术规范和安全要求的设备，提高操作人员的安全意识，加强安全知识的学习，提高作业水平，从而杜绝油罐汽车重大事故的发生。  相似文献