First discovery of a cave-dwelling Tineid moth （Lepidoptera,Tineidae） from East Asia     

Bong-Kyu Byun  ;Sat-Byul Shin  ;Yang-Seop Bae  ;Do Sung Kim  ;Yong Geun Choi 《林业研究》2014,25(3):647-651

In this study, we report Monopis crocicapitella （Clemens, 1859） （Tineidae）, which was collected from bat guano in a cave in the southern region of Korea, for the first time from East Asia. We briefly redescribe and illustrate the external morphology and genital structures of both sexes. Also, we discuss the local habitat characteristics and some of the ecological information that was observed during our field investi-gation.  相似文献   

Wear minimization of ultra high molecular weight polyethylene by benzophenone-assisted photocrosslinking     

Duek-Won Yun  Jinho Jang 《Fibers and Polymers》2014,15(3):480-486

Ultra high molecular weight polyethylene (UHMWPE) containing benzophenone (BP) is photocrosslinked under continuous UV irradiation. BP addition as low as 0.77 mol% per repeating unit of UHMWPE is enough to increase the gel fraction up to 99.8 %. The crosslinks are formed through the recombination of methine radicals generated from the hydrogen abstraction of UHMWPE by the excited BP. The heat of fusion of UHMWPE reduces substantially and the peak decomposition temperature increases as much as 15.8 oC due to the crosslinked network, coupled with slight decrease in crystallinity. The tensile strength, modulus and toughness of the crosslinked UHMWPE film are significantly improved with increasing crosslinking density. The wear rate of the UHMWPE also remarkably decreases from 10.2 mg/million cycles to 1.9 mg/million cycles, which can minimize the wear of total hip joint anthroplasty bearings.  相似文献   

Mucosa-associated lymphoid tissue lymphoma of the third eyelid conjunctiva in a dog     

Hong IH  Bae SH  Lee SG  Park JK  Ji AR  Ki MR  Han SY  Lee EM  Kim AY  You SY  Kim TH  Jeong KS 《Veterinary ophthalmology》2011,14(1):61-65

A 4-year-old, neutered female Cocker Spaniel was presented to the veterinary clinic for protrusion of the left third eyelid. When the third eyelids from both eyes were everted, lobulated masses were present on the bulbar surface. The left third eyelid had a larger protrusion. There was no apparent associated ocular or systemic involvement. The tumor of left third eyelid was removed and referred for histological examination. Histologically, there were proliferations of lymphoid follicles surrounded by lymphoid cells forming a marginal zone. Those lymphoid cells occasionally infiltrated into conjunctival epithelium. A few apoptotic bodies with karyopyknotic and karyorrhexic nuclei were observed in the germinal center of lymphoid follicles. Mitotic figures were rare. On immunohistochemistry, tumor cells expressed CD79a but not CD3. A diagnosis of extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT) of the third eyelid was established based on the histological and immunophenotypical features. At the 1-year follow-up, there was no evidence of recurrence of the mass at the area of excision of the left third eyelid and the remaining tumor of the right third eyelid was still a similar size. The dog still showed no significant findings, except those of the tumor, and no evidence of systemic involvement. To the authors' knowledge, this is the first reported case of MALT lymphoma of the third eyelid in a dog.  相似文献   

Mechanical properties and water-vapor permeability of soy-protein films affected by calcium salts and glucono-delta-lactone     

Park SK  Rhee CO  Bae DH  Hettiarachchy NS 《Journal of agricultural and food chemistry》2001,49(5):2308-2312

Edible films were prepared from solutions of soy protein with calcium salts and glucono-delta-lactone (GDL). Calcium salts cross-linking interactions with soy-protein isolate (SPI) could result in the formation of films with rigid three-dimensional structure. GDL contributed to the formation of a homogeneous film structure due to increased protein--solvent attraction. Tensile strength (TS) of calcium sulfate treated SPI film (8.6 MPa) was higher than the TS of calcium chloride treated SPI films (6.4 MPa) and the control SPI film (5.5 MPa). Puncture strength (PS) of calcium sulfate treated SPI film (9.8 MPa) was higher than the PS of calcium chloride treated SPI films (8.5 MPa) and the control SPI film (5.9 MPa). SPI film formulated with GDL had larger elongation at break (39.4%) than that of SPI control film (18.2%). Calcium salts and GDL-treated SPI films had lower water-vapor permeability than the SPI control film.  相似文献   

Statistical analysis of interactive cytotoxicity in human epidermal keratinocytes following exposure to a mixture of four metals     

Chris Gennings  Walter H. Carter  Julie A. Campain  Dong-soon Bae  Raymond S. H. Yang 《Journal of Agricultural, Biological & Environmental Statistics》2002,7(1):58-73

Exposure to mixtures of chemicals is an important and relevant environmental issue. Of particular interest is the detection and characterization of departure of biological effects from additivity. Methodology based on the assumption of additivity is used in fitting single-chemicaldata. Interactionsare determined and characterized by making comparisons between the observed and predicted responses at mixtures along a fixed ratio ray of the component substances. Two simultaneous tests are developed for testing for any departure from additivity. Multiple comparisons procedures are used to compare observed responses to that predicted under additivity. A simultaneous confidence band on the predicted responses along the mixture ray is also developed. The methods are illustrated with cytotoxicity data that arise when human epidermal keratinocytes are exposed to a mixture of arsenic, chromium, cadmium, and lead. Synergistic, antagonistic, and additive cytotoxicities were observed at different dose levels of the four-metal mixture.  相似文献   

Antiviral activity of Alpinia katsumadai extracts against rotaviruses     

Kim HH  Kwon HJ  Ryu YB  Chang JS  Cho KO  Hosmillo MD  Rho MC  Park SJ  Lee WS 《Research in veterinary science》2012,92(2):320-323

In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P[7] and porcine G5P[7] rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts [AK-1 (EtOH extract), AK-3 (H(2)O layer), AK-5 (40% methanol fraction), and AK-9-11 (H(2)O extract, polysaccharide fraction, supernatant fraction)] exhibited inhibitory activities against G5P[7] rotavirus with the EC(50) values ranging from 0.7±0.4 to 33.7±6.5 μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P[7] rotavirus, the with EC(50) values of 8.4±2.2 μg/mL, 6.5±0.8 μg/mL and 8.4±5.0 μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11 μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.  相似文献   

Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets   总被引：1，自引：0，他引：1  

Kim HJ  Park JG  Alfajaro MM  Kim DS  Hosmillo M  Son KY  Lee JH  Bae YC  Park SI  Kang MI  Cho KO 《Veterinary microbiology》2012,159(1-2):11-22

Rotaviruses are important human and animal pathogens with high impact on public health and livestock industry. There is little evidence about the cross-species pathogenicity and extra-intestinal infections of animal and human reassortant rotaviruses, particularly based on all 11 genotyping data. In this study, the bovine triple reassortant KJ56-1 strain harboring two bovine-like genome segments, eight porcine-like genome segments, and one human-like genome segment was used to evaluate the cross-species pathogenicity in its parent species, calves and piglets, and to determine its abilities of causing viremia and extra-intestinal tropisms in piglets. The KJ56-1 strain isolated from a calf diarrhea fecal sample replicated without causing diarrhea and severe intestinal pathology in calves. However, piglets inoculated with this strain showed persistent severe diarrhea and marked intestinal pathology. By SYBR Green real-time RT-PCR, viral RNA was detected in the sera, mesenteric lymph node, lung, liver, choroid plexus, and cerebrospinal fluid in the experimental piglets. An immunofluorescence assay confirmed viral replication in these extra-intestinal organs and tissues. These results indicated that the bovine triple reassortant KJ56-1 strain was virulent to piglets but not to calves. Our data also demonstrated that the reassortant rotaviruses had the ability to spread to the bloodstream from the gut, enter and amplify in the mesenteric lymph node, and disseminate to the extra-intestinal organs and tissues.  相似文献   

Mice immune responses to Brucella abortus heat shock proteins. Use of baculovirus recombinant-expressing whole insect cells,purified Brucella abortus recombinant proteins,and a vaccinia virus recombinant as immunogens     

Bae JE  Schurig GG  Toth TE 《Veterinary microbiology》2002,88(2):189-202

Brucella abortus resists the microbicidal mechanisms of macrophages, and the expression of its heat shock proteins (HSPs) such as GroEL, GroES and HtrA may play a role in this resistance. Bacterial HSPs can be very immunogenic, inducing protective immunity in various types of bacterial infections. However, the significance of immune responses directed against B. abortus HSPs in the protection against brucellosis is currently unresolved. To elucidate the role of these proteins in protection against Brucella challenge, individual, divalent or trivalent baculovirus (BV) recombinants of B. abortus GroEL, GroES and/or HtrA were injected into BALB/c mice either as protein-expressing whole cells or as purified proteins. The preparations were given to mice in combination with Freund's or Ribi adjuvant, respectively. In addition, some mice were primed with a vaccinia virus-GroEL recombinant, followed by inoculation with purified GroEL-Ribi adjuvant combination. Antibodies were observed against B. abortus GroEL and HtrA, but not against GroES. Cellular immune response was demonstrated by observing significant IFN-gamma release by lymphocytes of mice immunized with the purified HtrA-Ribi adjuvant combination. However, none of the mice inoculated with individual, divalent or trivalent HSP-expressing cells combined with complete Freund's adjuvant or inoculated with purified B. abortus HSPs combined with Ribi adjuvant, were protected against challenge with B. abortus virulent strain 2308. Priming with vaccinia virus-GroEL recombinant and boosting with GroEL-Ribi combination did not induce protective immunity. Based on the results obtained, we suggest that although humoral and cell-mediated immune responses are induced, but protective immune response is not induced by B. abortus HSPs.  相似文献   

Abundance of biting midge species (Diptera: Ceratopogonidae,Culicoides spp.) on cattle farms in Korea     

Jae-Ku Oem  Joon-Yee Chung  Mee-Soon Kwon  Toh-Kyung Kim  Tae-Uk Lee  You-Chan Bae 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(1):91-94

Culicoides biting midges were collected on three cattle farms weekly using light traps overnight from May to October between 2010 and 2011 in the southern part of Korea. The seasonal and geographical abundance of Culicodes spp. were measured. A total of 16,538 biting midges were collected from 2010 to 2011, including seven species of Culicoides, four of which represented 98.42% of the collected specimens. These four species were Culicodes (C.) punctatus (n = 14,413), C. arakawae (n = 1,120), C. oxystoma (n = 427), and C. maculatus (n = 318). C. punctatus was the predominant species (87.15%).  相似文献   

Amino acid substitutions in gyrA and parC associated with quinolone resistance in nalidixic acid-resistant Salmonella isolates     

Dong Hwa Bae  Ho Jeong Baek  So Jeong Jeong  Young Ju Lee 《Irish veterinary journal》2013,66(1):23

This study was undertaken to identify and characterize amino acid substitutions in gyrA and parC related with quinolone resistance of 27 nalidixic acid-resistant (Na^R) Salmonella isolates collected in poultry slaughterhouses in Korea. A total of 51 Salmonella isolates were detected from 44.8% (47/105) of the total samples from 15 poultry slaughterhouses examined, among which 27 (52.9%) Na^R isolates were detected while ciprofloxacin (Cip) resistance was not present in the isolates. These 27 Na^R isolates of DNA sequencing revealed that it contained three types of gyrA mutations in only D87 codon. Mutations in the D87 codon resulted in substitutions to G in most of the isolates, but D87Y and D87N exchanges were also detected. Although Cip resistance was absent, reduced susceptibility characterized by mutations in gyrA was apparent among Salmonella isolates from poultry slaughterhouses in Korea.  相似文献