Use of cDNA-AFLP for transcript profiling in narrow genetic pools; for example, cucumber (Cucumis sativus L.)   总被引：1，自引：1，他引：1  

K. M. Bae    Y. S. Kwon    I. H. Cho    S. I. Yi 《Plant Breeding》2006,125(5):488-492

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Yield of maize (Zea mays L.) logistically declined with increasing length of the consecutive visible wilting days during flowering     

Seonghyu Shin  Ju Seok Lee  Sang Gon Kim  Tae-Hoon Go  Jiyoung Shon  Shingu Kang  Jin-Seok Lee  Hwan Hee Bae  Jung-Tae Kim  Kang-Bo Shim  Woonho Yang  Mi-Ok Woo 《Journal of Crop Science and Biotechnology》2015,18(4):237-248

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<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> inbred line Bd21 with two binary vectors containing hygromycin resistance and GUS reporter genes     

Man Bo Lee  Woong Bae Jeon  Dae Yeon Kim  Odgerel Bold  Min Jeong Hong  Yong Jin Lee  Jae Han Park  Yong Weon Seo 《Journal of Crop Science and Biotechnology》2011,14(4):233-238

Brachypodium distachyon (Brachypodium) is a novel model plant for structural and functional genomic studies of temperate grasses. Brachypodium as a model plant has many favorable features, such as small size, small genome, short life cycle, self-fertility, and simple growth requirements. The genome sequence of the standard line Bd21 has been released and genomic resources have been developed.It is imperative to develop a method for efficient Agrobacterium-mediated Brachypodium transformation. Yellowish and compact embryogenic calli derived from immature embryos of the Bd21 were transformed with the Agrobacterium strain AGL1. Seven- and nine-week-old calli were used for transformation with Agrobacterium carrying either pCAMBIA 1301 and pCAUGH. Transformation efficiencies were assessed through histochemical GUS assay. The efficiency of transformation with pCAMBIA 1301 (based on the number of callus lines producing GUS-detected plantlets and the number of calli used for transformation) reached 20.1% (7-week-old calli) and 1.7% (9-week-old calli), and with pCAUGH (based on the number of GUS-detected plantlets and the number of regenerants) 90 and 87% for 7- and 9-week-old calli, respectively. High selection pressure was obtained by using pCAUGH, which is preferred for saving labor and time consumption during the callus selection.  相似文献   

H5N1 subtype highly pathogenic avian influenza virus isolated from healthy mallard captured in South Korea     

Kim HR  Kim BS  Bae YC  Moon OK  Oem JK  Kang HM  Choi JG  Lee OS  Lee YJ 《Veterinary microbiology》2011,151(3-4):386-389

On December 7, 2010, H5N1 highly pathogenic avian influenza virus was isolated from a healthy mallard captured at the Mankyung River in South Korea. Phylogenetic analysis showed that this virus was classified into clade 2.3.2 and closely related to H5N1 viruses isolated from wild birds in Mongolia, Russia and China in 2009 and 2010.  相似文献   

Production of conjugated linoleic acid (CLA) by Bifidobacterium breve LMC520 and its compatibility with CLA-producing rumen bacteria     

Park HG  Heo W  Kim SB  Kim HS  Bae GS  Chung SH  Seo HC  Kim YJ 《Journal of agricultural and food chemistry》2011,59(3):984-988

This study was performed to characterize the ability of an active Bifidobacterium strain to produce conjugated linoleic acid (CLA) and to test its possible utilization as a probiotic compatible to the ruminal condition. Bifidobacterium breve LMC520 can actively convert linoleic acid (LA) to cis-9,trans-11-CLA, which is a major isomer derived from microbial conversion. LMC520 showed reasonable tolerance under acidic conditions (pH 2.5 with 1% pepsin) and in the presence of oxgall (0-3%). The growth and CLA production of LMC520 were tested under ruminal conditions and compared with those of Butyrivibrio fibrisolvens A38, which is a major CLA producer in the rumen as an intermediate in the biohydrogenation (BH) process. LMC520 converted 15% of LA to CLA under ruminal conditions, which was 2 times higher activity than that of A38, and there was no decline in CLA level during prolonged incubation of 48 h. The BH activity of LMC520 was comparable to that of A38. When LMC520 was cocultured with A38, even with slight decrease of CLA due to high BH activity by A38, but the level of CLA was maintained by the high CLA-producing activity of LMC520. This comparative study shows the potential of this strain to be applied as a functional probiotic not only for humans but also for ruminants as well as to increase CLA production.  相似文献   

Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis   总被引：3，自引：0，他引：3  

Kim JW  Lee YJ  Han MY  Bae DH  Jung SC  Oh JS  Ha GW  Cho BK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(11):1103-1107

Canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. Diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. The objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ICA) for serodiagnosis of dogs suspected of having brucellosis, and results were compared with those obtained for hemoculture (HC) and the rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT). The all experimentally infected dogs were positive in ICA, HC and 2-ME RSAT from 5 weeks, 7 weeks, and 3 weeks after infection, respectively. Also, among dogs selected from 10 different breed kennels occurred brucellosis, 24.8%, 39.5% and 39.1% of dogs tested were detected as positive with HC, 2-ME RSAT and ICA, respectively. The kappa value between 2-ME RSAT and ICA was 0.89. The results of this study showed that sensitivity and specificity of the ICA are comparable with those obtained using conventional serological and bacteriological test for brucellosis. In conclusion, the ICA kit provides a handy and accurate tool for the rapid serodiagnosis of canine brucellosis.  相似文献   

Dissemination of antimicrobial resistant strains of Campylobacter coli and Campylobacter jejuni among cattle in Washington State and California     

Bae W  Hancock DD  Call DR  Park YH  Berge AC  Finger RM  Sischo WM  Besser TE 《Veterinary microbiology》2007,122(3-4):306-315

The purpose of this study was to investigate the genetic similarity of Campylobacter jejuni and Campylobacter coli with similar antimicrobial resistance phenotypes, isolated from cattle on different farms and at different times, in order to evaluate the possible existence of disseminated antimicrobial resistant clones. PFGE after SmaI and KpnI restriction identified 23 and 16 distinct PFGE patterns among 29 C. jejuni and 66 C. coli isolates, respectively. In C. coli, 51 (77%) of the resistant isolates demonstrated one of the four indistinguishable PFGE patterns, whereas only 24% doxycycline resistant C. jejuni shared one of the two indistinguishable PFGE patterns. The genetic mechanisms of resistance were homogeneous within and between these clonal types. Genetically indistinguishable (clonal) groups of C. coli accounted for most Campylobacter sp. with multiple antimicrobial resistance observed in this study, consistent with a role for clonal dissemination in the epidemiology of resistance in this species.  相似文献   

Freezing and Frost Damage of Potato Plants: a Case Study on Growth Recovery,Yield Response,and Quality Changes     

Dong Chil Chang  Hwang Bae Sohn  Ji Hong Cho  Ju Sung Im  Yong Ik Jin  Gyeong Ran Do  Su Jeong Kim  Hyun Mook Cho  Yong Beom Lee 《Potato Research》2014,57(2):99-110

The relationship between the severity of natural freezing and frost damage (FFD) and the observed growth recovery and field production of Superior potatoes (Solanum tuberosum L.) was investigated under screen house conditions. Potato plants were damaged by accidental freezing and frost during the early phase of shoot growth, 40 days after planting in highland summer crop areas in Korea. The observations of FFD were classified visually into “severe” (>75%), “moderate” (50%), “mild” (<25%), and “no FFD”, based on the percentage of the area of the shoots which was damaged. The early vegetative growth recovery, in terms of groundcover, was reduced as the level of FFD increased. At 28 days after a freezing and frost damage (DAFF), the potato plants with mild or no symptoms had produced more groundcover than the plants with moderate or severe symptoms. At 35 DAFF, groundcover was the same across all levels of severity, at nearly 100%. Despite the slower canopy development, plants with FFD achieved a higher fresh shoot weight and total chlorophyll content, whereas physiological growth indices such as photosynthetic rate and stomatal conductance of leaves were not different among levels of FFD at 60 DAFF. The number of lateral stems and days to tuberization increased significantly as the level of FFD increased; however, the harvest index, the number of tubers per plant, and seed tuber production were all reduced in plants with severe FFD. In addition, the tubers from plants with severe FFD had an increased ratio of length to width and 40% more tuber eyes than tubers from undamaged plants. The elongated tubers also showed an increase in cell division, demonstrated by higher numbers of cells in the cortical zones. The aforementioned measurements were obtained from natural event and led to a deduction that Superior has a capacity to recover from FFD, unless the plants were severely damaged (>75%) early in the season.  相似文献   

Bioactive Compounds,Antioxidant and Binding Activities and Spear Yield of Asparagus officinalis L.     

Jong Won Lee  Jeong Hyun Lee  In Ho Yu  Shela Gorinstein  Jong Hyang Bae  Yang Gyu Ku 《Plant foods for human nutrition (Dordrecht, Netherlands)》2014,69(2):175-181

The aim of this investigation was to find a proper harvesting period and establishing fern number, which effects the spear yield, bioactive compounds and antioxidant activities of Asparagus officinalis L. Spears were harvested at 2, 4, and 6 weeks after sprouting. Control for comparison was used without harvest. Spears and total yield increased with prolonged spear harvest period. In harvest of 6 weeks long optimum spear yield was the highest and fern numbers were 5?~?8. Bioactive compounds (polyphenols, flavonoids, flavanols, tannins and ascorbic acid) and the levels of antioxidant activities by ferric-reducing/antioxidant power (FRAP) and cupric reducing antioxidant capacity (CUPRAC) assays in asparagus ethanol extracts significantly differed in the investigated samples and were the highest at 6 weeks harvest period (P?<?0.05). The first and the second segments from the tip significantly increased with the increase of catalase (CAT). It was interesting to investigate in vitro how human serum albumin (HSA) interacts with polyphenols extracted from investigated vegetables. Therefore the functional properties of asparagus were studied by the interaction of polyphenol ethanol extracts with HSA, using 3D- FL. In conclusion, antioxidant status (bioactive compounds, binding and antioxidant activities) improved with the harvesting period and the first segment from spear tip. Appropriate harvesting is effective for higher asparagus yield and its bioactivity.  相似文献   

A New Benzofuran Glycoside and Indole Alkaloids from a Sponge-Associated Rare Actinomycete,Amycolatopsis sp.     

Yun Kwon  Seong-Hwan Kim  Yoonho Shin  Munhyung Bae  Byung-Yong Kim  Sang Kook Lee  Ki-Bong Oh  Jongheon Shin  Dong-Chan Oh 《Marine drugs》2014,12(4):2326-2340

Three new secondary metabolites, amycofuran (1), amycocyclopiazonic acid (2), and amycolactam (3), were isolated from the sponge-associated rare actinomycete Amycolatopsis sp. Based on combined spectroscopic analyses, the structures of 1–3 were determined to be a new benzofuran glycoside and new indole alkaloids related to cyclopiazonic acids, a class that has previously only been reported in fungi. The absolute configurations of 1 and 3 were deduced by ECD calculations, whereas that of 2 was determined using the modified Mosher method. Amycolactam (3) displayed significant cytotoxicity against the gastric cancer cell line SNU638 and the colon cancer cell line HCT116.  相似文献