LC-MS/MS quantification of sulforaphane and indole-3-carbinol metabolites in human plasma and urine after dietary intake of selenium-fortified broccoli     

Hauder J  Winkler S  Bub A  Rüfer CE  Pignitter M  Somoza V 《Journal of agricultural and food chemistry》2011,59(15):8047-8057

This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 μg of total glucosinolates and <0.01 μg of selenium per gram fresh weight) or selenium-fertilized broccoli (589 μg of total glucosinolates and 0.25 μg of selenium per gram fresh weight) was administered daily for 4 weeks. Glucoraphanin and glucobrassicin metabolites quantified in plasma and urine were SFN-glutathione, SFN-cysteine, SFN-cysteinylglycine, SFN-acetylcysteine, and indole-3-carboxaldehyde, indole-3-carboxylic acid, and ascorbigen, respectively. Dietary intake of selenium-fertilized broccoli increased serum selenium concentration analyzed by means of atomic absorption spectroscopy by up to 25% (p < 0.001), but affected neither glucosinolate concentrations in broccoli nor their metabolite concentrations in plasma and urine compared to regular broccoli.  相似文献   

Emergence of classical swine fever virus in Israel in 2009   总被引：1，自引：0，他引：1  

David D  Edri N  Yakobson BA  Bombarov V  King R  Davidson I  Pozzi P  Hadani Y  Bellaiche M  Schmeiser S  Perl S 《Veterinary journal (London, England : 1997)》2011,190(2):e146-e149

Classical swine fever (CSF) re-emerged in Israel in February 2009 after an absence of 62 years. The outbreak occurred on a domestic pig farm in northern Israel and affected domestic pigs and wild boar. On the basis of phylogenetic analysis of a 190 base pair fragment of the E2 glycoprotein gene, the Israeli CSF virus strain belonged to genotype 2.1 and was genetically most similar to a Chinese CSF virus strain.  相似文献   

Ultrasound findings in horses with severe eyelid swelling, and recognition of acute dacryoadenitis: 10 cases (2004-2010)     

Reimer JM  Latimer CS 《Veterinary ophthalmology》2011,14(2):86-92

Objective To report the sonographic findings, treatment, and outcome of horses with severe eyelid swelling, to describe the sonographic appearance of enlarged lacrimal glands, and to describe the clinical features of acute dacryoadenitis. Materials and methods Medical records of all horses with severe eyelid swelling that underwent an ultrasound evaluation of the globe and periorbital structures from 2004–2010 were examined. Cases were limited to those in which the eyelid swelling was so severe that the globe could not be visualized. Results Ten horses met the criteria for inclusion in the study. All cases were unilateral and acute. Marked enlargement of the lacrimal gland was found in five cases, and supported the diagnosis of acute dacryoadenitis. Eyelid abscessation was found in three horses, accompanied by mild to moderate lacrimal gland enlargement in two of the three. Enlarged lacrimal glands were slightly heterogeneous in appearance and could be differentiated from an abscess by imaging the protrusion of the gland from beneath the supraorbital rim. Only two cases of severe eyelid swelling were soley traumatic. Conclusions Severe eyelid swelling was often associated with dacryoadenitis and/or eyelid abscessation. The detection of marked lacrimal gland enlargement in horses with an acute onset of severe painful eyelid swelling is consistent with a diagnosis of acute dacryoadenitis. Ultrasonographic evaluation of eyelid swelling of any degree is warranted to determine if enlargement of the lacrimal gland, etiology notwithstanding, is an underlying or contributing cause.  相似文献   

FTIR-ATR-based prediction and modelling of lignin and energy contents reveals independent intra-specific variation of these traits in bioenergy poplars     

Guanwu Zhou  Gail Taylor  Andrea Polle 《Plant methods》2011,7(1):9

Background  

There is an increasing demand for renewable resources to replace fossil fuels. However, different applications such as the production of secondary biofuels or combustion for energy production require different wood properties. Therefore, high-throughput methods are needed for rapid screening of wood in large scale samples, e.g., to evaluate the outcome of tree breeding or genetic engineering. In this study, we investigated the intra-specific variability of lignin and energy contents in extractive-free wood of hybrid poplar progenies (Populus trichocarpa × deltoides) and tested if the range was sufficient for the development of quantitative prediction models based on Fourier transform infrared spectroscopy (FTIR). Since lignin is a major energy-bearing compound, we expected that the energy content of wood would be positively correlated with the lignin content.  相似文献   

A simple and efficient method for isolating small RNAs from different plant species     

FlordeFátima Rosas-Cárdenas  Noé Durán-Figueroa  Jean-Philippe Vielle-Calzada  Andrés Cruz-Hernández  Nayelli Marsch-Martínez  Stefan de Folter 《Plant methods》2011,7(1):4

Background  

Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging due to the composition of specific tissues of certain plant species. Here we describe a simple and efficient method to isolate small RNAs from different plant species.  相似文献   

Diofenolan: a novel insect growth regulator in common citrus butterfly, <Emphasis Type="Italic">Papilio demoleus</Emphasis>     

Satya Singh  Krishna Kumar 《Phytoparasitica》2011,39(3):205-213

Penultimate and last instar larvae of the common citrus swallowtail butterfly, Papilio demoleus L. (Papilionidae: Lepidoptera), were treated with different doses of a novel juvenoid, diofenolan. Several deformities were observed as a result of topical administration of diofenolan which include delay in larval–larval and larval–pupal ecdysis, ecdysial failure, mortality, severe reduction in pupation, deformed pupae and complete inhibition of adult emergence. The juvenoid diofenolan severely hampers the normal growth, development and metamorphosis of P. demoleus and can be incorporated into integrated pest management programs for successful control of this important pest of citrus all over the world.  相似文献   

Note on <Emphasis Type="Italic">Wahlgreniella nervata</Emphasis> (Gillette, 1908) (Hemiptera: Aphididae): a new pest of Damask rose in Turkey     

Shalva Barjadze  İsmail Karaca  Bülent Yaşar  Nana Gratiashvili 《Phytoparasitica》2011,39(3):239-241

Alate viviparous females of Wahlgreniella nervata (Gillette) were found on a Damask rose (Rosa damascena Mill.) plantation in Isparta Province, Turkey. The aphid is recorded for the first time from Turkey. Besides, Damask rose is a new host for this species. Brief information about this aphid including a key to the alate females of aphid species known to infest Damask rose in Turkey is provided.  相似文献   

Resistance of <Emphasis Type="BoldItalic">Spodoptera exigua</Emphasis> to ten insecticides in Shandong,China     

Chao Zhou  Yongqiang Liu  Weili Yu  Zhaorong Deng  Ming Gao  Feng Liu  Wei Mu 《Phytoparasitica》2011,39(4):315-324

Spodoptera exigua (Hübner) collected from three regions (two scallion-producing regions and one ginger-producing region) in Shandong, China and a laboratory colony of Wuhan (WHLC) were evaluated for their susceptibilities to ten insecticides (emamectin benzoate, chlorfenapyr, indoxacarb, spinosyn, tebufenozide, methoxyfenozide, chlorfluazuron, beta-cypermethrin, chlorpyrifos and methomyl) in 2008, 2009 and 2010 using a leaf-dip bioassay method. The results indicate that the resistance ratios of S. exigua to newer insecticides such as emamectin benzoate, chlorfenapyr and indoxacarb were all below 20-fold, with no obvious change in all 3 years. S. exigua exhibits moderate resistance to spinosyn, and its resistance ratios increased from 1.98–5.31-fold in 2008 to 14.31–64.20-fold in 2010 from three regions as compared with WHLC. S. exigua showed moderate to high resistance to insect growth regulators such as tebufenozide, methoxyfenozide, chlorfluazuron in 2008, 2009 and 2010, in which resistance to chlorfluazuron was rapidly increased from 31.49–88.19-fold in 2008 to 1184.39–2789.67-fold in 2010. Resistance of S. exigua to beta-cypermethrin and chlorpyrifos varied greatly among the three regions, ranging from 95.31–437.97-fold and 25.05–40.64-fold in 2008, 951.81–1304.40-fold and 44.91–186.33-fold in 2009, to 27.27–1095.31-fold and 19.12–267.98-fold in 2010. In contrast, S. exigua showed low resistance to methomyl, and the resistance ratio was below 5-fold in 3 years. There are several reasons accounting for varying degrees of resistance, including selection pressure, cropping structure and migration, in which the migration of S. exigua may play an important role.  相似文献   

Suppressive compost: when plant pathology met microbial ecology     

Yitzhak Hadar 《Phytoparasitica》2011,39(4):311-314

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Erratum to: Note on Wahlgreniella nervata (Gillette, 1908) (Hemiptera: Aphididae): a new pest of Damask rose in Turkey     

Shalva Barjadze  İsmail Karaca  Bülent Yaşar  Nana Gratiashvili 《Phytoparasitica》2011,39(3):309-309

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